Agilent Technologies 5185-5991 User manual

5,000 Molecular Weight Cut-Off (MWCO)
4 mL Spin Concentrator, Part No. 5185-5991
5185-5991 5 KDa MWCO 4 mL 5188-2798 5 KDa MWCO 15 mL
5188-5201 30 KDa MWCO 4 mL 5188-2799 30 KDa MWCO 15 mL
5188-5202 50 KDa MWCO 4 mL 5188-2800 50 KDa MWCO 15 mL
Agilent Spin Concentrators for Proteins
Instructions – 4 mL Concentrators
Equipment required 4 mL Spin Concentrators, pack of 25
Centrifuge
Rotor type Swing bucket or Fixed angle
Minimum rotor angle 25°
Rotor cavity To fit 15 mL (17 mm) conical bottom tubes
Minimum effective speed 2,000 g
Recommended speed 5,000 g
Maximum speed 7,500 g
Concentrate recovery
Pipette type Fixed or variable volume
Recommended tip Thin gel loader type
Technical specifications
Concentrator sample volume capacity
Swing bucket rotor 1-4 mL
Fixed angle rotor 1-4 mL
Dimensions
Total Length 122 mm
Width 17 mm
Active Membrane Area 2.0 cm2
Hold-up volume of membrane <10 µL
Dead stop volume 20 µL
pH range pH 1 – 9
Materials of construction
Body Polycarbonate
Filtrate vessel Polypropylene
Concentrator cap Polycarbonate
Membrane Polyethersulfone
Concentrating Proteins with 4 mL Concentrators:
1Fill concentrator with sample within the working volume
capacity range noted in the table above (ensure that the hinged
lid is fully seated).
2Insert assembled concentrator into centrifuge (when fixed
angle rotors are used, angle the concentrators so that the
printed windows face upwards/outwards).
3Centrifuge at recommended speeds, taking care not to exceed
the maximum g force indicated in the table above (20 minutes
at 7,500 g is recommended for 30´concentration of 4 mL at
10°C).
4Once the desired concentration is achieved, remove assembly
and recover sample from the bottom of the concentrate pocket
with a pipette.
Desalting/Buffer Exchange
1Concentrate sample to desired level or at least
5´.
2Empty filtrate container.
3Refill concentrator with an appropriate buffer.
4Concentrate the sample again and repeat the
process until the concentration of the
contaminating species is sufficiently reduced.
Typically three wash cycles will remove 99% of
initial salt content.
See Notes on next page.

30,000 Molecular Weight Cut-Off (MWCO)
15 mL Spin Concentrator, Part No. 5188-2799
05/2004A
Instructions – 15 mL Concentrators
Equipment required 15 mL Spin Concentrators, pack of 10
Centrifuge
Rotor type Swing bucket or Fixed angle
Minimum rotor angle 25°
Rotor cavity To fit 30 x 114 mm conical bottom tubes or
29 x 104 mm round bottom tubes (50mL)
Minimum effective speed 1,000 g
Recommended speed 2,000 g
Maximum speed 3,000 g
Concentrate recovery
Pipette type Fixed or variable volume
Recommended tip Thin gel loader type
Technical specifications
Concentrator sample volume capacity
Swing bucket rotor 2-15 mL
Fixed angle rotor 2-8 mL
Dimensions
Total Length 76 mm
Width 25.5 mm
Active Membrane Area 4 cm2
Hold-up volume of membrane <20 µL
Dead stop volume 50 µL
pH range pH 1 – 9
Materials of construction
Body Polycarbonate
Filtrate vessel Polypropylene
Concentrator cap Polycarbonate
Membrane Polyethersulfone
Concentrating Proteins with 15 mL Concentrators:
1Fill concentrator with sample within the working volume
capacity range noted in the table above, taking care not to
touch the membrane surface, and then close the hinged lid
(ensure that the lid is fully seated).
2Place concentrator into 50 mL centrifuge tube (filtrate
collection vessel – not supplied, user must provide).
3Insert assembled concentrator into centrifuge (when fixed
angle rotors are used, angle the concentrators so that the
printed windows face upwards/outwards). Spin at
recommended speeds, taking care not to exceed the maximum
g force indicated in the table above (20-40 minutes is
common).
4Once the desired concentration is achieved, remove assembly
and recover sample from the bottom of the concentrate pocket
with a pipette.
Desalting/Buffer Exchange
See 4 mL Spin Concentrator instructions on the reverse side.
Notes:
·Spin concentrators are single use disposable
items. Re-use is not recommended.
·For research use only.
·Select the most appropriate membrane cut-off for
your sample. For maximum recovery, select a
MWCO at least 50% smaller than the molecular
size of the species of interest.
·These concentrators are designed for use with
biological fluids or aqueous solutions only, not
for organic solvents or strong acids or bases.
·Flow rates are affected by MWCO, porosity,
sample size, concentration, viscosity, centrifuge
force, and temperature. Adjust centrifuge force
and time accordingly for your samples.
·Do not autoclave. Sterilize with a 70% ethanol
solution.
For more information or technical assistance, Agilen
t
Technologies, Inc.
please call toll free: 1-800-227-9770 or visit 2850 Centerville Road
our web site at: www.agilent.com/chem/bioreagents Wilmington, DE 19808-1610 USA