Agilent Technologies 8453 User manual

s1
Multicell Transport for
Agilent 8453 UV-visible
Spectrophotometer
Operator’s Manual

Agilent Technologies Deutschland GmbH
Hewlett-Packard-Strasse 8
76337 Waldbronn
Germany
Copyright Agilent
Technologies 2000
All rights reserved.
Reproduction, adaption,
or translation without
prior written permission
is prohibited, except as
allowed under the
copyright laws.
Part No. G1120-90001
Edition 02/00
Printed in Germany
Warranty
The information
contained in this
document is subject to
change without notice.
Agilent Technologies
makes no warranty of
any kind with regard to
this material,
including, but not
limited to, the implied
warranties or
merchantability and
fitness for a particular
purpose.
Agilent Technologies
shall not be liable for
errors contained herein
or for incidental or
consequential damages
in connection with the
furnishing, performance,
or use of this material.
WARNING
For details of safety,
see Safety Information
on page 48.
Warning Symbols Used
In This Book
The apparatus is marked
with this symbol when
the user should refer to
the instruction manual
in order to protect the
apparatus against
damage.
!

Operator’s Manual
Multicell Transport for Agilent 8453 UV-visible
Spectrophotometer

4
In This Book
This handbook contains specifications of the eight-position multicell
transport (order number G1120A) and describes how install this accessory in
an Agilent 8453 UV-visible spectrophotometer. This handbook also includes
operating guidelines for checking out and using the multicell transport. For
details of how to operate the multicell transport through the Agilent
ChemStation, refer to the help system.
For service personnel there is additional information on maintenance,
adjustments, troubleshooting and repair. Part numbers are given for
replacement and exchange parts with an exploded view for better
identification of the individual part.
The eight-position multicell transport is not compatible with older Hewlett-
Packard spectrophotometers such as the HP 8450, HP 8451 and HP 8452A.
To control the multicell transport through the Agilent ChemStation, the
software must be revision A.05.xx or higher.
• Chapter 1 “Description and Specifications” gives you specifications of the
multicell transport.
•Chapter 2 “Installing and Operating Your Multicell Transport”describes
how to install the multicell transport in your spectrophotometer and how
to do an an operational checkout.
•Chapter 3 “Diagnostics and Troubleshooting”for some hints about
cleaning the multicell transport and for troubleshooting.
•Chapter 4 “Maintenance and Repair”describes simple maintenance tasks,
repair procedures, and the adjustments that are necessary after you have
made a repair.
•Chapter 5 “Parts and Supplies”helps you to locate parts and find out their
part numbers.

Contents
5
1 Description and Specifications
An overview of the multicell transport accessory and its
specifications 7
Description 8
Specifications 10
2 Installing and Operating Your Multicell Transport
How to install, operate, and do an operational checkout 11
Site Requirements for Your Multicell Transport 13
Unpacking Your Multicell Transport 14
Installing Your Multicell Transport 15
Installing the Stirring Module 18
Installing the Filter Kit 20
Operating Your Multicell Transport 23
Removing the Multicell Transport from the
Spectrophotometer 25
To Remove the Cell Holder 26
3 Diagnostics and Troubleshooting
Descriptions of error messages, general troubleshooting hints,
and how to check the electronics 27
Error Messages 28
General Troubleshooting Hints 29
Checking the Electronics 31

6
Contents
4 Maintenance and Repair
Simple maintenance tasks, repair procedures, and adjust-
ments that are necessary after you have made a repair 33
Simple Maintenance 35
Replacing the Plastic Cover 36
Adjusting the Belt Tension 38
5 Parts and Supplies
Figures and tables to help you identify part numbers of the
multicell transport and related supplies 39

1
1Description and
Specifications
An overview of the multicell transport accessory
and its specifications

8
Description and Specifications
Description
Description
The eight-position multicell transport (order number G1120A) is an
accessory for the Agilent 8453 UV-visible spectrophotometer. It is a
multiple-sample holder with multiple-position capability, see Figure 1. The
multicell transport cell holder holds eight cells in a rack which can be held at
a constant temperature with water circulated from a thermostatic bath
(provided by the user and not supplied by Agilent Technologies).
Figure 1 The Eight-position Multicell Transport
The holder is attached to the multicell transport base which contains a
stepper motor to move the designated cell into the optical path. A
water-driven magnetic stirrer can be fitted under the holder in one of two
positions—either under the cell in the optical path or under the cell to the left
of the cell in the optical path.
The multicell transport can be controlled by any Agilent Technologies
UV-visible operating software, having revision A.05.xx or higher, through the
accessory interface of the spectrophotometer.
NOTE The eight-position multicell transport can not be controlled by Agilent
Technologies UV-visible operating software with revision A.02.05 or lower.
Cell Holder
Tubing Clamp
Stirring module with
cell-stirring water-supply
(optional)
Connection for
water bath

9
Description and Specifications
Description
Cell Stirring Module
The cell stirring module is mounted in the multicell transport base to provide
magnetic-induction stirring in the cell at or adjacent to the measurement
position. The cell stirring module contains a plastic-cased magnetic impeller
that may be driven by pressurized water. When a magnetic stirring bar (part
number 9301-1161) has been inserted in a solvent-filled, standard 1 cm
cuvette the stirring bar rotates in tandem with the impeller magnet to
produce solution stirring.
Cell Holder
The cell holder has a capacity for eight cells. The outside dimensions of
individual cells must be 12.5 ×12.5 mm and the height from base of the cell to
the sample area 15 mm.
During an analysis, the position of individual cells is maintained by a flat
spring inside each of the cell compartments. The spring alone fixes the cell.
No additional fixing mechanism is needed, see Figure 1.
Optical Filter Kit
An optional optical filter kit (part number G1120-68707) may be used with the
cell holder to modify the incident light at the sample during a measurement in
case of highly photosensitive samples.

10
Description and Specifications
Specifications
Specifications
The multicell transport generally operates under the same environmental
conditions as those specified in the Reference Handbook for the Agilent 8453
spectrophotometer. A maximum water pressure of approximately 3.5 bar
(50 psi) can be supplied to the thermostatted cell holder and to the
water-driven stirrer.
Table 1 Multicell Transport Performance Specifications
Item Specification Comment
Time to move between adjacent cell
positions
< 1 second
Position repeatability ± 0.1 mm Linear position error in moving to
a cell position within 1 hour
Step resolution 0.060 mm
Temperature range for thermostatting
(cell holder only)
5–90 °C
Temperature difference between any
two cell positions
0.3 °C With a water bath at 37 °C