Agilent Technologies InfinityLab LC Series User manual

Diode Array Detectors
Agilent InfinityLab LC Series
User Manual
Agilent InfinityLab LC Series Diode Array Detectors User Manual

Agilent InfinityLab LC Series Diode Array Detectors User Manual
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EDITION 09/2020
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Agilent InfinityLab LC Series Diode Array Detectors User Manual 3
In This Book
This manual covers the Agilent 1290 Infinity II Diode Array Detectors
•G7117A - 1290 Infinity II Diode Array Detector FS (with fixed slit)
•G7117B - 1290 Infinity II Diode Array Detector (with variable slit)
•G7117C - 1260 Infinity II Diode Array Detector HS (with fixed slit)
Find information on other Agilent Diode Array Detectors in separate manuals.
1 Introduction
This chapter gives an introduction to the detector and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Module
This chapter explains the essential operational parameters of the module.
4 Preparing the Module
This chapter provides information on how to set up the module for an analysis and
explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 4
8 Test Functions and Calibration
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200 Infinity
Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 5
Contents
1 Introduction 9
Overview of the Module 10
Product Description 11
Optical System 14
Leak and Waste Handling 19
Operating Principle 24
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
3 Using the Module 37
Magnets 38
Turn on/off 39
Status Indicators 41
Instrument Configuration 42
Set up the Detector with Agilent Open Lab ChemStation 44
The Detector User Interface 45
Detector Control Settings 47
Method Parameter Settings 48
4 Preparing the Module 52
Leak and Waste Handling 53
Setting up an Analysis 55
Solvent Information 62
Capillary Coding Guide 68
Installing Capillaries 71

Agilent InfinityLab LC Series Diode Array Detectors User Manual 6
5 Optimizing the Detector 75
Introduction 76
Optimization Overview 77
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 83
Optimizing Selectivity 94
Optimizing the Detector Regarding to the System 96
Warm up of the Detector 102
6 Troubleshooting and Diagnostics 104
Available Tests vs User Interfaces 105
Agilent Lab Advisor Software 107
7 Error Information 108
What Are Error Messages 109
General Error Messages 110
Detector Error Messages 116
8 Test Functions and Calibration 121
Introduction 122
Use of Max-Light Cartridge Test Cell 126
Conditions of Detector 127
Failing a Test 127
Self-Test 128
Intensity Test 130
Cell Test 132
Quick Noise Test 134
ASTM Drift and Noise Test 137
Slit Test 140
Wavelength Verification Test 143
Wavelength Calibration 145
D/A Converter (DAC) Test 148
Dark Current Test 150
Other Lab Advisor Functions 152

Agilent InfinityLab LC Series Diode Array Detectors User Manual 7
9 Maintenance 153
Introduction to Maintenance 154
Warnings and Cautions 154
Overview of Maintenance 156
Cleaning the Module 157
Remove and Install Doors 157
Replace the Deuterium Lamp 159
Replace the Max-Light Cartridge Cell 162
Clean the Max-Light Cartridge Cell 165
Storage of Max-Light Cartridge Cell 166
Correcting Leaks 167
Replace Leak Handling System Parts 168
Replace the Module Firmware 170
Information from Module’s Assemblies 171
10 Parts and Materials for Maintenance 172
Overview of Maintenance Parts 173
Accessory Kit 174
11 Identifying Cables 175
Cable Overview 176
Analog Cables 178
Remote Cables 180
CAN/LAN Cables 183
RS-232 Cables 184
USB Cables 184
12 Hardware Information 185
Firmware Description 186
Electrical Connections 189
Interfaces 192
Setting the 6-bit Configuration Switch 199
Instrument Layout 202
Early Maintenance Feedback 203

Agilent InfinityLab LC Series Diode Array Detectors User Manual 8
13 LAN Configuration 205
What You Have to Do First 206
TCP/IP parameter configuration 207
Configuration Switches 208
Initialization Mode Selection 209
Dynamic Host Configuration Protocol (DHCP) 211
Manual Configuration 214
14 Appendix 219
General Safety Information 220
Waste Electrical and Electronic Equipment (WEEE) Directive 226
Radio Interference 227
Sound Emission 227
Solvent Information 228
Agilent Technologies on Internet 229

Agilent InfinityLab LC Series Diode Array Detectors User Manual 9
1 Introduction
Overview of the Module 10
Product Description 11
Product Description G7117A 11
Product Description G7117B 12
Product Description G7117C 13
Optical System 14
Lamp 15
Max-Light Cartridge Flow Cell 15
Slit Assembly 16
Grating and Diode Array 18
Leak and Waste Handling 19
Waste Concept 23
Operating Principle 24
This chapter gives an introduction to the detector and an instrument overview.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 10
1 Introduction
Overview of the Module
Overview of the Module
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•Maximum of 120 Hz (G7117A/G7117C) or 240 Hz (G7117B) data acquisition
rate.
•Higher sensitivity for conventional LC as well as ultra fast applications by
using next generation optical design.
•Increased sensitivity with 60 mm Max-Light cartridge flow cell.
•Optimized cell geometry for less peak dispersion for narrow bore applications.
•Max-Light cartridge flow cells for standard applications are available, see
“Max-Light Cartridge Flow Cell” on page 15.
•More reliable and robust peak integration process (automated) due to less
baseline noise/drift/refractive index and thermal effects especially under ultra
fast gradient conditions.
•RFID tracking technology is used for the UV-lamp and the Max-Light cartridge
flow cells.
•Multiple wavelength and full spectral detection at 120 Hz
(G7117A/G7117C)/240 Hz (G7117B) sampling rate, keeping up with the
analysis speed of ultra-fast LC.
•Programmable 1 – 8 nm slit (G7117B) or fixed 4 nm slit (G7117A/C) for rapid
optimization of sensitivity, linearity and spectral resolution provides optimum
incident light conditions.
•Improved Electronic temperature control (ETC) provides maximum baseline
stability and practical sensitivity under fluctuating ambient temperature and
humidity conditions.
•Additional diagnostic signals for temperature and lamp voltage monitoring.
•Easy exchange of flow cell by cartridge design.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 11
1 Introduction
Product Description
Product Description
Product Description G7117A
The Agilent 1290 Infinity II Diode Array Detector FS (fixed slit) is based on the
Agilent Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100% efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 µAU/cm the 60 mm flow cell gives up
to 10 times higher sensitivity than detectors with conventional flow cells.
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 120 Hz.
Figure 1 Overview of the detector
Status indicator
Lamp housing cover
Cartridge Flow Cell
Power switch
Leak drain
Air inlet

Agilent InfinityLab LC Series Diode Array Detectors User Manual 12
1 Introduction
Product Description
Product Description G7117B
The Agilent 1290 Infinity II Diode Array Detector (DAD) is based on the Agilent
Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100% efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 µAU/cm the 60 mm flow cell gives up
to 10 times higher sensitivity than detectors with conventional flow cells.
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 240 Hz.
Figure 2 Overview of the detector
Status indicator
Lamp housing cover
Cartridge Flow Cell
Power switch
Leak drain
Air inlet

Agilent InfinityLab LC Series Diode Array Detectors User Manual 13
1 Introduction
Product Description
Product Description G7117C
The Agilent 1260 Infinity II Diode Array Detector HS with fixed slit is based on the
Agilent Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100% efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 µAU/cm the 60 mm flow cell gives up
to 10 times higher sensitivity than detectors with conventional flow cells.
Figure 3 Overview of the detector
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 120 Hz.
Status indicator
Lamp housing cover
Cartridge Flow Cell
Power switch
Leak drain
Air inlet

Agilent InfinityLab LC Series Diode Array Detectors User Manual 14
1 Introduction
Optical System
Optical System
The optical system of the detector is shown in Figure 4 on page 14.
Figure 4 Optical System of the Detector
The illumination source is a deuterium-arc-discharge lamp [1] for the ultraviolet
(UV) wavelength range. Its light is focused by a lamp mirror [2] onto the entrance
of the Max-light cartridge flow cell [3] with optofluidic waveguides. The light
leaves the Max-light cartridge flow cell at the other side and is focused by the fold
mirror [4] through the slit assembly [5] onto a holographic grating [6] light being
dispersed onto the diode array [7]. This allows simultaneous access to all
wavelength information.
1 UV-lamp
2 Lamp mirror
3 Flow cell
4 Fold mirror
5 Programmable (G7117B) or Fixed (G7117A/G7117C) slit
6 Grating
7 Array

Agilent InfinityLab LC Series Diode Array Detectors User Manual 15
1 Introduction
Optical System
Lamp
The light source for the UV-wavelength range is a long-life UV-lamp with RFID
tag. As a result of plasma discharge in low-pressure deuterium gas, the lamp
emits light over the 190 nm to approximately 800 nm wavelength range.
Figure 5 UV-Lamp
Max-Light Cartridge Flow Cell
The detector allows easy access to flow cells via a cartridge. A variety of optional
flow cells can be inserted using the same quick, simple mounting system.
Max-Light Cartridge Flow Cells for standard and bio-inert applications are
available. For testing of the detector, a Max-Light Cartridge Test Cell is available.
The optical principle of the Max-Light Cartridge cell is based on opto-fluidic
waveguides. Nearly 100 % light transmission is achieved by utilizing total internal
reflection in a non-coated silica fiber. Compromising refractive index and thermal
effects are almost completely eliminated, resulting in significantly less baseline
drift.
p/n Description
G4212-60008 Max-Light Cartridge Cell (10 mm, V() 1.0 µL)
G4212-60007 Max-Light Cartridge Cell (60 mm, V() 4.0 µL)
G4212-60032 HDR Max-Light Cartridge Cell (3.7 mm, V() 0.4 µL)
G4212-60038 ULD Max-Light Cartridge Cell (10 mm, V() 0.6 µL)
G7117-60020 Max-Light Cartridge Cell LSS (10 mm, V() 1.0 µL)
G4212-60011 Max-Light Cartridge Test Cell

Agilent InfinityLab LC Series Diode Array Detectors User Manual 16
1 Introduction
Optical System
Figure 6 Max-Light Cartridge Flow Cell
Slit Assembly
Programmable Slit (G7117B)
The micro-slit system makes use of the mechanical properties of silicon
combined with the precise structuring capabilities of bulk micro-machining. It
combines the required optical functions — slit and shutter — in a simple and
compact component. The slit width is directly controlled by the micro-processor
of the instrument and can be set as method parameter.
Figure 7 Slit Assembly
The slit width influences the spectral resolution and noise.
NOTE For additional information on the Max-Light Cartridge flow cell refer to “Choosing
a Flow Cell” on page 78 and “Inline Pressure Relief Valve Kit (G4212-68001)” on
page 81.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 17
1 Introduction
Optical System
Figure 8 Influence of slitwidth on resolution and noise level
Fixed Slit (G7117A/G7117C)
The fixed slit combines the required optical functions - slit and shutter - in a
simple and compact component. The slit width is fixed to 4 nm and both
positions (fixed slit and shutter) are directly controlled by the micro- processor of
the instrument.

Agilent InfinityLab LC Series Diode Array Detectors User Manual 18
1 Introduction
Optical System
Grating and Diode Array
The combination of dispersion and spectral imaging is accomplished by using a
concave holographic grating. The grating separates the light beam into all its
component wavelengths and reflects the light onto the photodiode array.
The diode array is a series of 1024 individual photodiodes and control circuits
located on a ceramic carrier. It has a wavelength range from 190 – 640 nm and
the sampling interval is 0.5 nm.
Figure 9 Grating and diode array

Agilent InfinityLab LC Series Diode Array Detectors User Manual 19
1 Introduction
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve

Agilent InfinityLab LC Series Diode Array Detectors User Manual 20
1 Introduction
Leak and Waste Handling
Figure 10 Infinity II Leak Waste Concept (Flex Bench installation)