Agilent Technologies 1200 Infinity Series User manual

Agilent Technologies
Agilent 1200 Infinity Series
Variable Wavelength
Detectors
User Manual

Agilent 1200 Infinity Series VWD - User Manual
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Manual Part Number
G1314-90034 Rev B
Edition
11/2013
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Agilent 1200 Infinity Series VWD - User Manual 3
In This Guide
In This Guide
This manual covers
•the Agilent 1290 Infinity Variable Wavelength Detector (G1314E),
•the Agilent 1260 Infinity Variable Wavelength Detector (G1314F) and
•the Agilent 1200 series Variable Wavelength Detector (G1314D)
(obsolete).
Find information on other Agilent Variable Wavelength Detectors in
separate manuals.
1 Introduction to the Variable Wavelength Detector
This chapter gives an introduction to the detector, instrument overview
and internal connectors.
2 Site Requirements and Specifications
This chapter gives information on environmental requirements, physical
and performance specifications.
3 Installing the Detector
This chapter describes the installation of the detector.
4 Using the Detector
This chapter provides information on how to set up the detector for an
analysis and explains the basic settings.
5 How to optimize the detector
This chapter gives hints on how to select the detector parameters and the
flow cell.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.

4 Agilent 1200 Infinity Series VWD - User Manual
In This Guide
7 Error Information
This chapter describes the meaning of detector error messages, and
provides information on probable causes and suggested actions how to
recover from error conditions.
8TestFunctions
This chapter describes the detector’s built in test functions.
9 Maintenance and Repair
This chapter provides general information on maintenance and repair of
the detector.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the detector to the
Agilent ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1200 Infinity Series VWD - User Manual 5
Contents
Contents
1 Introduction to the Variable Wavelength Detector 9
Introduction to the Detector 10
Optical System Overview 11
System Overview 16
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications G1314D 24
Performance Specifications G1314E 27
Performance Specifications G1314F 30
3 Installing the Detector 33
Unpacking the Detector 34
Optimizing the Stack Configuration 36
Installation Information on Leak and Waste Handling 45
Installing the Detector 49
Flow Connections to the Detector 52
4 Using the Detector 55
Leak and Waste Handling 56
Setting up an Analysis 57
Special Settings of the Detector 71
5 How to optimize the detector 83
Optimizing the Detector Performance 84
Match the Flow Cell to the Column 85
Set the Detector Parameters 88

6 Agilent 1200 Infinity Series VWD - User Manual
Contents
6 Troubleshooting and Diagnostics 89
Overview of the Detector’s Indicators and Test Functions 90
Status Indicators 91
Available Tests versus Interfaces 93
Agilent Lab Advisor Software 94
7 Error Information 95
What Are Error Messages 97
General Error Messages 98
Detector Error Messages 104
8 Test Functions 115
Intensity Test 116
Cell Test 118
Wavelength Verification-Calibration 120
ASTM Drift and Noise Test 122
Quick Noise Test 123
Dark Current Test 124
Holmium Oxide Test 126
9 Maintenance and Repair 129
Introduction to Maintenance 130
Warnings and Cautions 131
Overview of Maintenance 133
Cleaning the Module 134
Exchanging a Lamp 135
Exchanging a Flow Cell 138
Repairing the Flow Cells 140
Using the Cuvette Holder 143
Correcting Leaks 145
Replacing Leak Handling System Parts 146
Replacing the Module’s Firmware 147

Agilent 1200 Infinity Series VWD - User Manual 7
Contents
10 Parts and Materials for Maintenance 149
Overview of Maintenance Parts 150
Standard Flow Cell 10 mm / 14 µL 152
Micro Flow Cell 3 mm / 2 µL 154
Semi-micro Flow Cell 6 mm / 5 µL 156
High Pressure Flow Cell 10 mm / 14 µL 158
Cuvette Holder 160
Kits 151
Leak Parts 161
11 Identifying Cables 163
Cable Overview 164
Analog Cables 166
Remote Cables 168
BCD Cables 171
CAN/LAN Cables 173
RS-232 Cables 174
12 Hardware Information 175
Firmware Description 176
Electrical Connections 179
Interfaces 182
Setting the 8-bit Configuration Switch 189
Instrument Layout 193
Early Maintenance Feedback (EMF) 194
13 LAN Configuration 197
What you have to do first 198
TCP-IP parameter configuration 199
Configuration Switches 200
Initialization mode selection 201
Dynamic Host Configuration Protocol (DHCP) 205
Link configuration selection 208
Automatic configuration with Bootp 209
Manual Configuration 219
PC and User Interface Software Setup 225

8 Agilent 1200 Infinity Series VWD - User Manual
Contents
14 Appendix 227
General Safety Information 228
Radio Interference 231
Sound Emission 232
UV Radiation 233
Solvent Information 234
Declaration of Conformity for HOX2 Filter 236
Agilent Technologies on Internet 237

9
Agilent 1200 Infinity Series VWD - User Manual
Agilent Technologies
1
Introduction to the Variable Wavelength
Detector
Introduction to the Detector 10
Optical System Overview 11
Flow Cell 12
Lamp 13
Source Lens Assembly 13
Entrance Slit Assembly 13
Filter Assembly 14
Mirror Assemblies M1 and M2 15
Grating Assembly 15
Beam Splitter Assembly 15
Photo Diodes Assemblies 15
Photo Diode ADC (analog-to-digital converter) 15
System Overview 16
Leak and Waste Handling 16
This chapter gives an introduction to the detector, instrument overview and
internal connectors.

10 Agilent 1200 Infinity Series VWD - User Manual
1Introduction to the Variable Wavelength Detector
Introduction to the Detector
Introduction to the Detector
The Agilent variable wavelength detectors described in this manual are
designed for highest optical performance, GLP compliance and easy
maintenance with:
•data rate up to 20 Hz for standard- HPLC (G1314D), see Table 10 on
page 78
•higher data rate up to 80 Hz for fast- HPLC (G1314F), see Table 11 on
page 78
•higher data rate up to 160 Hz for ultra- fast- HPLC (G1314E), see
Table 12 on page 79
•data recovery card DRC (G1314E) gives unique "data- never- lost"
insurance “Run Recovery Settings” on page 80,
•deuterium lamp for highest intensity and lowest detection limit over a
wavelength range of 190 to 600 nm,
•optional flow- cell cartridges (standard 10 mm, 14 µL; high pressure
10 mm, 14 µL; micro 3 mm, 2 µL; semi- micro 6 mm, 5 µL) are
available and can be used depending on the application needs (other
types may be introduced later),
•easy front access to lamp and flow cell for fast replacement,
•electronic identification of flow cell and lamp with RFID (Radio
Frequency Identification) tag for unambiguous identification,
•lamp information: part number, serial number, production date,
ignitions, burn time
•cell information: part number, serial number, production date,
nominal path length, volume, maximum pressure
•built- in electronic temperature control (ETC) for improved baseline
stability, and
•built- in holmium oxide filter for fast wavelength accuracy verification.
For specifications refer to Table 3 on page 24.

Agilent 1200 Infinity Series VWD - User Manual 11
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Optical System Overview
The optical system of the detector is shown in the figure below. Its
radiation source is a deuterium- arc discharge lamp for the ultraviolet (UV)
wavelength range from 190 to 600 nm. The light beam from the deuterium
lamp passes through a lens, a filter assembly, an entrance slit, a spherical
mirror (M1), a grating, a second spherical mirror (M2), a beam splitter,
and finally through a flow cell to the sample diode. The beam through the
flow cell is absorbed depending on the solutions in the cell, in which UV
absorption takes place, and the intensity is converted to an electrical
signal by means of the sample photodiode. Part of the light is directed to
the reference photodiode by the beam splitter to obtain a reference signal
for compensation of intensity fluctuation of the light source. A slit in front
of the reference photodiode cuts out light of the sample bandwidth.
Wavelength selection is made by rotating the grating, which is driven
directly by a stepper motor. This configuration allows fast change of the
wavelength. The cutoff filter is moved into the lightpath above 370 nm to
reduce higher order light.
Figure 1 Optical Path of the Variable Wavelength Detector
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12 Agilent 1200 Infinity Series VWD - User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Flow Cell
A variety of flow- cell cartridges can be inserted using the same quick and
simple mounting system.
The flow cells have an integrated RFID tag that contains the flow cell
specific information (e.g. part number, cell volume, path length, ...). A
RFID tag reader reads out this information and transfers it to the user
interface.
Figure 2 Flow Cell with RFID tag
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Table 1 Flow Cell Data
STD Semi-micro Micro High Pressure
Maximum pressure 40 (4) 40 (4) 120 (12) 400 (40) bar
Path length 10 (conical) 6 (conical) 3 (conical) 10 (conical) mm
Volume 14 5 2 14 µL
Inlet i.d. 0.25 0.17 0.12 0.25 mm
Inlet length 750 250 310 750 mm
Outlet i.d. 0.30 0.17 0.17 0.17 mm
Outlet length 120 120 120 120 mm
Total volume 60.77 14.49 14.00 60.77 µL
Materials in contact
with solvent
SST, quartz, PTFE,
PEEK
SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton

Agilent 1200 Infinity Series VWD - User Manual 13
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Lamp
The light source for the UV wavelength range is a deuterium lamp. As a
result of plasma discharge in a low pressure deuterium gas, the lamp
emits light over the 190 – 600 nm wavelength range.
The lamp has an integrated RFID tag that contains the lamp specific
information (e.g. part number, burn time, ...). A RFID tag reader reads out
this information and transfers it to the user interface.
Source Lens Assembly
The source lens receives the light from the deuterium lamp and focuses it
onto the entrance slit.
Entrance Slit Assembly
The entrance slit assembly has an exchangeable slit. The standard one has
a 1- mm slit. For replacement and calibration purposes to optimize the
alignment, a slit with a hole is needed.

14 Agilent 1200 Infinity Series VWD - User Manual
1Introduction to the Variable Wavelength Detector
Optical System Overview
Filter Assembly
The filter assembly is electromechanically actuated. During wavelength
calibrations it moves into the light path.
Figure 3 Filter Assemby
The filter assembly has two filters installed and is processor- controlled.
A photo sensor determines the correct position.
OPEN nothing in light path at λ<370nm
CUTOFF cut off filter in light path at λ>370nm
HOLMIU
M
holmium oxide filter for wavelength check
SHUTTER for measurement of dark current of photo
diodes
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Agilent 1200 Infinity Series VWD - User Manual 15
Introduction to the Variable Wavelength Detector 1
Optical System Overview
Mirror Assemblies M1 and M2
The instrument contains two spherical mirrors (M1 and M2). The beam
adjustable is vertically and horizontally. Both mirrors are identical.
Grating Assembly
The grating separates the light beam into all its component wavelengths
and reflects the light onto mirror #2.
The stepper motor reference position is determined by a plate fitted onto
the motor shaft, interrupting the beam of a photo sensor. The wavelength
calibration of the grating is done at the zero order light position and at
656 nm, which is the emission line of the deuterium lamp.
Beam Splitter Assembly
The beam splitter splits the light beam. One part goes directly to the
sample diode. The other part of the light beam goes to the reference
diode.
Photo Diodes Assemblies
Two photo diode assemblies are installed in the optical unit. The sample
diode assembly is located on the left side of the optical unit. The
reference diode assembly is located in the front of the optical unit.
Photo Diode ADC (analog-to-digital converter)
The photo diode current is directly converted to digital data direct photo
current digitalization. The data is transferred to the detector main board .
The photo diode ADC boards are located close to the photo diodes.

16 Agilent 1200 Infinity Series VWD - User Manual
1Introduction to the Variable Wavelength Detector
System Overview
System Overview
Leak and Waste Handling
The 1200 Infinity Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.

Agilent 1200 Infinity Series VWD - User Manual 17
Introduction to the Variable Wavelength Detector 1
System Overview
Figure 4 Leak and waste handling concept (overview - typical stack configuration as an
example)
The solvent cabinet (1) is designed to store a maximum volume of 6 L
solvent. The maximum volume for an individual bottle stored in the
solvent cabinet should not exceed 2.5 L. For details, see the usage
guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed
copy of the guideline has been shipped with the solvent cabinet, electronic
copies are available on the Internet).
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18 Agilent 1200 Infinity Series VWD - User Manual
1Introduction to the Variable Wavelength Detector
System Overview
The leak pan (2) (individually designed in each module) guides solvents to
the front of the module. The concept covers also leakages on internal
parts (e.g. the detector’s flow cell). The leak sensor in the leak pan stops
the running system as soon as the leak detection level is reached.
The leak pan's outlet port (3, A) guides excessive overfill from one module
to the next, as the solvent flows into the next module’s leak funnel (3, B)
and the connected corrugated waste tube (3, C). The corrugated waste
tube guides the solvent to the next lower positioned module’s leak tray
and sensor.
The waste tube of the sampler’s needle wash port (4) guides solvents to
waste.
The condense drain outlet of the autosampler cooler (5) guides condensate
to waste.
The waste tube of the purge valve (6) guides solvents to waste.
The waste tube connected to the leak pan outlet on each of the bottom
instruments (7) guides the solvent to a suitable waste container.

19
Agilent 1200 Infinity Series VWD - User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 20
Physical Specifications 23
Performance Specifications G1314D 24
Performance Specifications G1314E 27
Performance Specifications G1314F 30
This chapter gives information on environmental requirements, physical and
performance specifications.

20 Agilent 1200 Infinity Series VWD - User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Consideration
The detector power supply has wide ranging capabilities, see “Physical
Specifications” on page 23. It accepts any line voltage in the above
mentioned range. Consequently, there is no voltage selector in the rear of
the detector. There are also no externally accessible fuses, because
automatic electronic fuses are implemented in the power supply.
WARNING
Instrument is partially energized when switched off
The power supply still uses some power, even when the power switch on the front
panel is turned OFF. Repair work at the detector can lead to personal injuries, e. g.
shock hazard, when the detector cover is opened and the instrument is connected to
power.
➔To disconnect the detector from the power line, unplug the power cord.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
CAUTION
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.