Agilent Technologies 1200 Infinity Series User manual

Agilent Technologies
Agilent 1200 Infinity Series
Diode Array Detectors
User Manual

Agilent 1200 Infinity Series DAD User Manual
Notices
© Agilent Technologies, Inc. 2010-2011,
2012, 2013
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Manual Part Number
G4212-90013
Edition
02/2013
Printed in Germany
Agilent Technologies
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Otherwise, it is intended only for gen-
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Agilent 1200 Infinity Series DAD User Manual 3
In This Book
In This Book
This manual covers
•the Agilent 1290 Infinity Diode Array Detector (G4212A) and
•the Agilent 1260 Infinity Diode Array Detector (G4212B).
Find information on other Agilent Diode Array Detectors in separate manuals.
1 Introduction
This chapter gives an introduction to the detector and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the module.
4 Using the Module
This chapter provides information on how to set up the module for an analysis
and explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1200 Infinity Series DAD User Manual
In This Book
8 Test Functions and Calibration
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1260
Infinity/1290 Infinity LC modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1200 Infinity Series DAD User Manual 5
Contents
Contents
1 Introduction 9
Overview of the Module 10
Optical System 11
Bio-inert Materials 17
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Installing the Module 29
Unpacking the Module 30
Optimizing the Stack Configuration 32
Installing the Detector 41
Flow Connections to the Detector 44
Initial Recalibration 48
4 Using the Module 49
Preparing the Detector 50
Setting up the Detector with Agilent ChemStation 51
Main Screens of the Detector with Agilent Instant Pilot (G4208A) 69
Solvent Information 73
5 Optimizing the Detector 77
Introduction 78
Optimization Overview 79
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 85
Optimizing Selectivity 96
Optimizing the Detector Regarding to the System 100
Warm up of the Detector 107

6 Agilent 1200 Infinity Series DAD User Manual
Contents
6 Troubleshooting and Diagnostics 109
Overview of the Module’s Indicators and Test Functions 110
Status Indicators 111
Available Tests vs User Interfaces 113
Agilent Lab Advisor Software 114
Intermittent Issues 115
Board Type does not match when replacing the main board 116
7 Error Information 117
What Are Error Messages 118
General Error Messages 119
Detector Error Messages 126
8 Test Functions and Calibration 133
Introduction 134
Use of Max-Light Cartridge Test Cell 135
Conditions of Detector 136
Failing a Test 137
Self-Test 138
Intensity Test 140
Cell Test 143
Quick Noise Test 145
ASTM Drift and Noise Test 148
Slit Test 151
Wavelength Verification Test 154
Wavelength Calibration 156
D/A Converter (DAC) Test 159
Dark Current Test 161
9 Maintenance 163
Warnings and Cautions 164
Introduction to Maintenance 166
Overview of Maintenance 167
Cleaning the Module 168
Replacing the Deuterium Lamp 169
Replacing the Max-Light Cartridge Cell 173
Cleaning of Max-Light Cartridge Cell 178

Agilent 1200 Infinity Series DAD User Manual 7
Contents
Storage of Max-Light Cartridge Cell 179
Drying the Leak Sensor 180
Replacing Leak Handling System Parts 181
Replacing Module Firmware 183
Information from Module’s Assemblies 185
10 Parts and Materials for Maintenance 187
Overview of Maintenance Parts 188
Kits 190
11 Identifying Cables 191
Cable Overview 192
Analog Cables 194
Remote Cables 196
BCD Cables 199
CAN/LAN Cables 201
RS-232 Cables 202
12 Hardware Information 203
Firmware Description 204
Electrical Connections 207
Interfaces 210
Setting the 8-bit Configuration Switch 217
Instrument Layout 220
Early Maintenance Feedback 221
13 LAN Configuration 223
What You Have to Do First 224
TCP/IP parameter configuration 225
Configuration Switch 226
Initialization mode selection 227
Dynamic Host Configuration Protocol (DHCP) 231
Link configuration selection 234
Automatic Configuration with BootP 235
Manual Configuration 245
PC and Agilent ChemStation Setup 251

8 Agilent 1200 Infinity Series DAD User Manual
Contents
14 Appendix 261
Safety 262
The Waste Electrical and Electronic Equipment Directive 266
Radio Interference 267
Sound Emission 268
Solvent Information 269
Agilent Technologies on Internet 270

9
Agilent 1200 Infinity Series DAD User Manual
Agilent Technologies
1
Introduction
Overview of the Module 10
Optical System 11
Lamp 12
Max-Light Cartridge Flow Cell 13
Slit Assembly 14
Grating and Diode Array 16
Bio-inert Materials 17
This chapter gives an introduction to the detector and an instrument overview.

10 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Overview of the Module
Overview of the Module
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•Maximum of 160 Hz (G4212A) or 80 Hz (G4212B) data acquisition rate.
•Higher sensitivity for conventional LC as well as ultra fast applications by
using next generation optical design.
•Increased sensitivity with 60 mm Max-Light cartridge flow cell.
•Optimized cell geometry for less peak dispersion for narrow bore
applications.
•Max-Light cartridge flow cells for standard and bio-inert applications are
available.
•More reliable and robust peak integration process (automated) due to less
baseline noise/drift/refractive index and thermal effects especially under
ultra fast gradient conditions.
•RFID tracking technology is used for the UV-lamp and the Max-Light
cartridge flow cells.
•Multiple wavelength and full spectral detection at 160 Hz (G4212A)/80 Hz
(G4212B) sampling rate, keeping up with the analysis speed of ultra-fast
LC.
•Programmable 1 – 8 nm slit (G4212A) or fixed 4 nm slit (G4212B) for rapid
optimization of sensitivity, linearity and spectral resolution provides
optimum incident light conditions.
•Improved Electronic temperature control (ETC) provides maximum
baseline stability and practical sensitivity under fluctuating ambient
temperature and humidity conditions.
•Additional diagnostic signals for temperature and lamp voltage monitoring.
•Easy exchange of flow cell by cartridge design.
For specifications, see “Performance Specifications G4212A” on page 24 or
“Performance Specifications G4212B” on page 26.