Agilent Technologies 1200 Infinity Series User manual

Agilent Technologies
Agilent 1200 Infinity Series
Diode Array Detectors
User Manual

Agilent 1200 Infinity Series DAD User Manual
Notices
© Agilent Technologies, Inc. 2010-2011,
2012, 2013
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Manual Part Number
G4212-90013
Edition
02/2013
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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stood and met.

Agilent 1200 Infinity Series DAD User Manual 3
In This Book
In This Book
This manual covers
•the Agilent 1290 Infinity Diode Array Detector (G4212A) and
•the Agilent 1260 Infinity Diode Array Detector (G4212B).
Find information on other Agilent Diode Array Detectors in separate manuals.
1 Introduction
This chapter gives an introduction to the detector and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter provides information on unpacking, checking on completeness,
stack considerations and installation of the module.
4 Using the Module
This chapter provides information on how to set up the module for an analysis
and explains the basic settings.
5 Optimizing the Detector
This chapter provides information on how to optimize the detector.
6 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent 1200 Infinity Series DAD User Manual
In This Book
8 Test Functions and Calibration
This chapter describes the tests for the module.
9 Maintenance
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
11 Identifying Cables
This chapter provides information on cables used with the Agilent 1260
Infinity/1290 Infinity LC modules.
12 Hardware Information
This chapter describes the detector in more detail on hardware and
electronics.
13 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
14 Appendix
This chapter provides addition information on safety, legal and web.

Agilent 1200 Infinity Series DAD User Manual 5
Contents
Contents
1 Introduction 9
Overview of the Module 10
Optical System 11
Bio-inert Materials 17
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3 Installing the Module 29
Unpacking the Module 30
Optimizing the Stack Configuration 32
Installing the Detector 41
Flow Connections to the Detector 44
Initial Recalibration 48
4 Using the Module 49
Preparing the Detector 50
Setting up the Detector with Agilent ChemStation 51
Main Screens of the Detector with Agilent Instant Pilot (G4208A) 69
Solvent Information 73
5 Optimizing the Detector 77
Introduction 78
Optimization Overview 79
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 85
Optimizing Selectivity 96
Optimizing the Detector Regarding to the System 100
Warm up of the Detector 107

6 Agilent 1200 Infinity Series DAD User Manual
Contents
6 Troubleshooting and Diagnostics 109
Overview of the Module’s Indicators and Test Functions 110
Status Indicators 111
Available Tests vs User Interfaces 113
Agilent Lab Advisor Software 114
Intermittent Issues 115
Board Type does not match when replacing the main board 116
7 Error Information 117
What Are Error Messages 118
General Error Messages 119
Detector Error Messages 126
8 Test Functions and Calibration 133
Introduction 134
Use of Max-Light Cartridge Test Cell 135
Conditions of Detector 136
Failing a Test 137
Self-Test 138
Intensity Test 140
Cell Test 143
Quick Noise Test 145
ASTM Drift and Noise Test 148
Slit Test 151
Wavelength Verification Test 154
Wavelength Calibration 156
D/A Converter (DAC) Test 159
Dark Current Test 161
9 Maintenance 163
Warnings and Cautions 164
Introduction to Maintenance 166
Overview of Maintenance 167
Cleaning the Module 168
Replacing the Deuterium Lamp 169
Replacing the Max-Light Cartridge Cell 173
Cleaning of Max-Light Cartridge Cell 178

Agilent 1200 Infinity Series DAD User Manual 7
Contents
Storage of Max-Light Cartridge Cell 179
Drying the Leak Sensor 180
Replacing Leak Handling System Parts 181
Replacing Module Firmware 183
Information from Module’s Assemblies 185
10 Parts and Materials for Maintenance 187
Overview of Maintenance Parts 188
Kits 190
11 Identifying Cables 191
Cable Overview 192
Analog Cables 194
Remote Cables 196
BCD Cables 199
CAN/LAN Cables 201
RS-232 Cables 202
12 Hardware Information 203
Firmware Description 204
Electrical Connections 207
Interfaces 210
Setting the 8-bit Configuration Switch 217
Instrument Layout 220
Early Maintenance Feedback 221
13 LAN Configuration 223
What You Have to Do First 224
TCP/IP parameter configuration 225
Configuration Switch 226
Initialization mode selection 227
Dynamic Host Configuration Protocol (DHCP) 231
Link configuration selection 234
Automatic Configuration with BootP 235
Manual Configuration 245
PC and Agilent ChemStation Setup 251

8 Agilent 1200 Infinity Series DAD User Manual
Contents
14 Appendix 261
Safety 262
The Waste Electrical and Electronic Equipment Directive 266
Radio Interference 267
Sound Emission 268
Solvent Information 269
Agilent Technologies on Internet 270

9
Agilent 1200 Infinity Series DAD User Manual
Agilent Technologies
1
Introduction
Overview of the Module 10
Optical System 11
Lamp 12
Max-Light Cartridge Flow Cell 13
Slit Assembly 14
Grating and Diode Array 16
Bio-inert Materials 17
This chapter gives an introduction to the detector and an instrument overview.

10 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Overview of the Module
Overview of the Module
The detector is designed for highest optical performance, GLP compliance and
easy maintenance. It includes the following features:
•Maximum of 160 Hz (G4212A) or 80 Hz (G4212B) data acquisition rate.
•Higher sensitivity for conventional LC as well as ultra fast applications by
using next generation optical design.
•Increased sensitivity with 60 mm Max-Light cartridge flow cell.
•Optimized cell geometry for less peak dispersion for narrow bore
applications.
•Max-Light cartridge flow cells for standard and bio-inert applications are
available.
•More reliable and robust peak integration process (automated) due to less
baseline noise/drift/refractive index and thermal effects especially under
ultra fast gradient conditions.
•RFID tracking technology is used for the UV-lamp and the Max-Light
cartridge flow cells.
•Multiple wavelength and full spectral detection at 160 Hz (G4212A)/80 Hz
(G4212B) sampling rate, keeping up with the analysis speed of ultra-fast
LC.
•Programmable 1 – 8 nm slit (G4212A) or fixed 4 nm slit (G4212B) for rapid
optimization of sensitivity, linearity and spectral resolution provides
optimum incident light conditions.
•Improved Electronic temperature control (ETC) provides maximum
baseline stability and practical sensitivity under fluctuating ambient
temperature and humidity conditions.
•Additional diagnostic signals for temperature and lamp voltage monitoring.
•Easy exchange of flow cell by cartridge design.
For specifications, see “Performance Specifications G4212A” on page 24 or
“Performance Specifications G4212B” on page 26.

Agilent 1200 Infinity Series DAD User Manual 11
Introduction 1
Optical System
Optical System
The optical system of the detector is shown in Figure 1 on page 11.
Figure 1 Optical System of the Detector
The illumination source is a deuterium-arc-discharge lamp [1] for the
ultraviolet (UV) wavelength range. Its light is focused by a lamp mirror [2]
onto the entrance of the Max-light cartridge flow cell [3] with optofluidic
waveguides. The light leaves the Max-light cartridge flow cell at the other side
and is focused by the fold mirror [4] through the slit assembly [5] onto a
holographic grating [6] light being dispersed onto the diode array [7]. This
allows simultaneous access to all wavelength information.
1UV-lamp
2 Lamp mirror
3Flowcell
4 Fold mirror
5 Programmable (G4212A) or Fixed (G4212B) slit
6Grating
7 Array

12 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Optical System
Lamp
The light source for the UV-wavelength range is a long-life UV-lamp with RFID
tag. As a result of plasma discharge in low-pressure deuterium gas, the lamp
emits light over the 190 nm to approximately 800 nm wavelength range.
Figure 2 UV-Lamp

Agilent 1200 Infinity Series DAD User Manual 13
Introduction 1
Optical System
Max-Light Cartridge Flow Cell
The detector allows easy access to flow cells via a cartridge. A variety of
optional flow cells can be inserted using the same quick, simple mounting
system.
Max-Light Cartridge Flow Cells for standard and bio-inert applications are
available. For testing of the detector, a Max-Light Cartridge Test Cell is
available.
The optical principle of the Max-Light Cartridge cell is based on opto-fluidic
waveguides. Nearly 100 % light transmission is achieved by utilizing total
internal reflection in a non-coated silica fiber. Compromising refractive index
and thermal effects are almost completely eliminated, resulting in significantly
less baseline drift.
Figure 3 Max-Light Cartridge Flow Cell
p/n Description
G4212-60008 Max-Light Cartridge Cell (10 mm, V(σ) 1.0 µL)
G4212-60007 Max-Light Cartridge Cell (60 mm, V(σ) 4.0 µL)
G5615-60018 Max-Light Cartridge Cell Bio-inert (10 mm, V(σ) 1.0 µL)
includes Peek Capillary 1.5 m i.d. 0.18 mm (0890-1763) and PEEK Fittings 10/PK (5063-6591)
G5615-60017 Max-Light Cartridge Cell Bio-inert (60 mm, V(σ) 4.0 µL)
includes Peek Capillary 1.5 m i.d. 0.18 mm (0890-1763) and PEEK Fittings 10/PK (5063-6591)
G4212-60032 HDR Max-Light Cartridge Cell (3.7 mm, V(σ) 0.4 µL)
G4212-60038 ULD Max-Light Cartridge Cell (10 mm, V(σ) 0.6 µL)
G4212-60011 Max-Light Cartridge Test Cell
NOTE
For additional information on the Max-Light Cartridge flow cell refer to “Choosing a Flow
Cell” on page 80 and “Inline Pressure Relief Valve Kit (G4212-68001)” on page 82.

14 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Optical System
Slit Assembly
Programmable Slit (G4212A)
The micro-slit system makes use of the mechanical properties of silicon
combined with the precise structuring capabilities of bulk micro-machining. It
combines the required optical functions — slit and shutter — in a simple and
compact component. The slit width is directly controlled by the
micro-processor of the instrument and can be set as method parameter.
Figure 4 Slit Assembly
The slit width influences the spectral resolution and noise.

Agilent 1200 Infinity Series DAD User Manual 15
Introduction 1
Optical System
Figure 5 Influence of slitwidth on resolution and noise level
Fixed Slit (G4212B)
The fixed slit combines the required optical functions - slit and shutter - in a
simple and compact component. The slit width is directly controlled by the
micro-processor of the instrument and is fixed to 4 nm.
NOTE
In March 2011 the optical unit type of the G4212B DAD changed from "Programmable Slit
(like in the G4212A) fixed to 4 nm" to a real "Fixed Slit 4 nm". First serial number was
DEAA301100.

16 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Optical System
Grating and Diode Array
The combination of dispersion and spectral imaging is accomplished by using
a concave holographic grating. The grating separates the light beam into all its
component wavelengths and reflects the light onto the photodiode array.
The diode array is a series of 1024 individual photodiodes and control circuits
located on a ceramic carrier. It has a wavelength range from 190 – 640 nm and
the sampling interval is ∼0.5 nm.
Figure 6 Grating and diode array

Agilent 1200 Infinity Series DAD User Manual 17
Introduction 1
Bio-inert Materials
Bio-inert Materials
For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses
highest quality materials in the flow path (also referred to as wetted parts),
which are widely accepted by life scientists, as they are known for optimum
inertness to biological samples and ensure best compatibility with common
samples and solvents over a wide pH range. Explicitly, the complete flow path
is free of stainless steel and free of other alloys containing metals such as iron,
nickel, cobalt, chromium, molybdenum or copper, which can interfere with
biological samples. The flow downstream of the sample introduction contains
no metals whatsoever.
Max-Light Cartridge Cell Bio-inert ( 60 mm, V(σ) 4.0 μL) (G5615-60017) and
Max-Light Cartridge Cell Bio-inert ( 10 mm, V(σ) 1.0 μL) (G5615-60018) offer
highest sensitivity for bio-inert reverse phase applications. Please note that at
low salt SEC or ion exchange chromatography potentially peak tailing might
occur, and therefore for these applications the universal Bio-inert DAD
(G1315C or D) or MWD (G1365C or D) is recommended.
Table 1 Bio-inert materials used in Agilent 1260 Infinity Systems
Module Materials
Agilent 1260 Infinity Bio-inert Quaternary Pump
(G5611A)
Titanium, gold, platinum-iridium,
ceramic, ruby, PTFE, PEEK
Agilent 1260 Infinity Bio-inert High-Performance Autosampler
(G5667A)
Upstream of sample introduction:
•Titanium, gold, PTFE, PEEK,
ceramic
Downstream of sample
introduction:
•PEEK, ceramic
Agilent 1260 Infinity Bio-inert Manual Injector
(G5628A)
PEEK, ceramic
Agilent 1260 Infinity Bio-inert Analytical Fraction Collector
(G5664A)
PEEK, ceramic, PTFE

18 Agilent 1200 Infinity Series DAD User Manual
1Introduction
Bio-inert Materials
Bio-inert Flow Cells:
Standard flow cell bio-inert, 10 mm, 13 µL, 120 bar ( 12 MPa)
for MWD/DAD, includes Capillary Kit Flow Cells BIO
(p/n G5615-68755) (G5615-60022)
(for Agilent 1260 Infinity Diode Array Detectors DAD
G1315C/D)
PEEK, ceramic, sapphire, PTFE
Max-Light Cartridge Cell Bio-inert ( 10 mm, V(σ) 1.0 µL)
(G5615-60018) and
Max-Light Cartridge Cell Bio-inert ( 60 mm, V(σ) 4.0 µL)
(G5615-60017)
(for Agilent 1200 Infinity Series Diode Array Detectors DAD
G4212A/B)
PEEK, fused silica
Bio-inert flow cell, 8 µL, 20 bar (pH 1–12) includes Capillary Kit
Flow Cells BIO (p/n G5615-68755) (G5615-60005)
(for Agilent 1260 Infinity Fluorescence Detector FLD G1321B)
PEEK, fused silica, PTFE
Bio-inert heat-exchanger G5616-60050
(for Agilent 1290 Infinity Thermostatted Column Compartment
G1316C)
PEEK (steel-cladded)
Bio-inert Valve heads G4235A, G5631A, G5639A: PEEK,
ceramic (Al2O3based)
Bio-inert Connection capillaries Upstream of sample introduction:
•Titanium
Downstream of sample
introduction:
•Agilent uses
stainless-steel-cladded PEEK
capillaries, which keep the flow
path free of steel and provide
pressure stability to more than
600 bar.
Table 1 Bio-inert materials used in Agilent 1260 Infinity Systems
Module Materials
NOTE
To ensure optimum bio-compatibility of your Agilent 1260 Infinity Bio-inert LC system, do
not include non-inert standard modules or parts to the flow path. Do not use any parts that
are not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see
“Solvent information for parts of the 1260 Infinity Bio-inert LC system” on page 73.

19
Agilent 1200 Infinity Series DAD User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
Specifications 24
Specification Conditions 28
This chapter provides information on environmental requirements, physical and
performance specifications.

20 Agilent 1200 Infinity Series DAD User Manual
2Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
module.
Power Consideration
The module power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 2 on page 23. Consequently, there is
no voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. shock hazard, when the
cover is opened and the module is connected to power.
➔Make sure that it is always possible to access the power plug.
➔Remove the power cable from the instrument before opening the cover.
➔Do not connect the power cable to the Instrument while the covers are removed.
WARNING
Incorrect line voltage at the module
Shock hazard or damage of your instrument can result if the devices are connected
to line voltage higher than specified.
➔Connect your module to the specified line voltage.
CAUTION
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the power
line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.