Agilent Technologies 1100 Series User manual

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Agilent 1100 Series
Diode Array and
Multiple Wavelength
Detectors
Reference Manual

2 1100 Series DAD and MWD Reference Manual
Notices
© Agilent Technologies, Inc. 1995,
1996-2004
No part of this manual may be reproduced in
any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
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Manual Part Number
G1315-90005
Edition
Edition 05/2004
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Software Revision
This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Diode Array and
Multiple Wavelength Detectors software,
where xx refers to minor revisions of the
software that do not affect the technical
accuracy of this guide.
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ard. It calls attention to an operat-
ing procedure, practice, or the like
that, if not correctly performed or
adhered to, could result in damage
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conditions are fully understood and
met.
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
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notice until the indicated condi-
tions are fully understood and
met.

1100 Series DAD and MWD Reference Manual 3
In This Guide…
This manual contains information for using your Diode Array
and Multiple Wavelength Detectors.
1 Installing the Detector
How to install the detector
2 How to optmize the Detector
How to opimize the detector
3 Troubleshooting and Test Functions
The detector’s built-in troubleshooting and test functions
4 Repairing the Detector
Instructions on how to repair the detector
5 Identifying Parts and Materials
Detailed illustrations and listings for parts and materials
identification for the detector
6 Introduction to the Detector
An introduction to the detector, istrument overview, theory of
operation, external communication and internal connectors
7 Control Module Screens for Agilent 1100 DAD and MWD
This chapter is intended to introduce an operator to the screens
available for operation of the Agilent 1100 diode array detector
(DAD) with the Agilent 1100 control module.
8 Specifications
Performance specifications of the detector
Annex A Safety Information

4 1100 Series DAD and MWD Reference Manual

1100 Series DAD and MWD Reference Manual 5
Contents
1 Installing the Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Damaged Packaging 17
Delivery Checklist 17
Optimizing the Stack Configuration 20
Installing the Detector 22
Flow Connections to the Detector 25
2 How to optmize the Detector
Optimizing the Detector Performance 30
Optimization Overview 31
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 33
Flow Cell Path Length 33
Peak width (response time) 35
Sample and Reference Wavelength and Bandwidth 36
Slit Width 39
Optimizing Spectral Acquisition (DAD only) 42
Margin for Negative Absorbance 43
Optimizing Selectivity 44
Quantifying Coeluting Peaks by Peak Suppression 44
Ratio Qualifiers for Selective Detection of Compound
Classes 46

6 1100 Series DAD and MWD Reference Manual
3 Troubleshooting and Test Functions
Overview of the Detector’s Indicators and Test Functions 50
Status Indicators 51
Power Supply Indicator 51
Detector Status Indicator 51
Error Messages 53
Timeout 54
Shutdown 55
Remote Timeout 56
Synchronization Lost 57
Leak 58
Leak Sensor Open 60
Leak Sensor Short 61
Compensation Sensor Open 62
Compensation Sensor Short 63
Fan Failed 64
Open Cover 65
Cover Violation 66
Visible Lamp Current 67
Visible Lamp Voltage 68
Communication Error 69
Calibration Values Invalid 70
Diode Current Leakage 71
Holmium Oxide Test Failed 72
UV Lamp Current 73
UV Lamp Voltage 74
UV Ignition Failed 75
UV Heater Current 76
New Messages with the G1315B and G1365B detectors 77
Module Type Changed 78
Illegal Value From Temperature Sensor Mounted On The Fan
Assembly 80

1100 Series DAD and MWD Reference Manual 7
Illegal Value From „Air Inlet" Temperature Sensor 81
Heater Failed 82
Heater Power At Limit 83
Wavelength Recalibration 84
Test Functions 85
Holmium Oxide Test 86
Intensity Test 88
Cell Test 90
Dark-Current Test 91
Filter Test 93
4 Repairing the Detector
Introduction into Repairing the Detector 96
Using the ESD Strap 98
Overview of the Repairing of the Detector 99
Simple Repairs 101
Exchanging a Lamp 102
Exchanging a Flow Cell 105
Repairing the Standard or Semi-Micro Flow Cell 108
Repairing the High Pressure Flow Cell 112
Replacing Capillaries on a Standard Flow Cell 114
Replacing Capillaries on a Semi-Micro and High Pressure Flow
Cell 119
Nano Flow Cell - Replacing or Cleaning 122
Cleaning or Exchanging the Holmium Oxide Filter 127
Correcting Leaks 130
Replacing Leak Handling System Parts 131
Cleaning or Replacing Coupling Lens Assembly 132
Cleaning or Replacing Cell Support Window 135
Exchanging Internal Parts 138
Removing the Top Cover and Foam 140

8 1100 Series DAD and MWD Reference Manual
Exchanging the Detector Main Board 143
Entering the Serial Number using the Control Module 146
Entering the Serial Number using the
Agilent ChemStation 147
Exchanging the Fan (G1315A/G1365A) 148
Replacing Fan Parts (G1315B/G1365B) 150
Removing the Optical Unit (G1315A/G1365A) 154
Removing the Optical Unit (G1315B/G1365B) 156
Exchanging the Source Lens (Achromat) Assembly 158
Replacing the Holmium Oxide Filter Motor 162
Exchanging the Power Supply 164
Exchanging the Leak Sensor 167
Replacing Status Light Pipe 170
Installing the Optical Unit (G1315A/G1365A) 171
Installing the Optical Unit (G1315B/G1365B) 173
Replacing the Foam and the Top Cover 175
Assembling the Main Cover 178
Replacing the Interface Board 179
Replacing the Detector’s Firmware 180
5 Identifying Parts and Materials
Overview of Main Assemblies 182
Optical Unit Assembly 184
Standard Flow Cell 186
Semi-Micro Flow Cell 188
High Pressure Flow Cell 190
Nano Flow Cells 192
Fan Assembly Parts 196
Holmium Oxide Filter 197
Coupling Lens Assembly 198

1100 Series DAD and MWD Reference Manual 9
Source Lens (Achromat) Assembly 199
Cell Support Assembly 200
Control Module 201
Sheet Metal Kit (G1315A/G1365A) 202
Sheet Metal Kit (G1315B/G1365B) 203
Plastic Parts 204
Foam Parts 205
Power and Status Light Pipes 206
Leak Parts 207
Accessory Kit 208
Cable Overview 210
Analog Cables 212
Remote Cables 215
BCD Cables 220
Auxiliary Cable 222
CAN Cable 223
External Contact Cable 224
RS-232 Cable Kit 225
LAN Cables 226
6 Introduction to the Detector
Introduction to the Detector 228
Optical System Overview 229
Electrical Connections 232
Instrument Layout 234
Early Maintenance Feedback (EMF) 235
EMF Counters 235

10 1100 Series DAD and MWD Reference Manual
Using the EMF Counters 235
Electronics 237
Detector Main Board (DAM/MWM) 238
Firmware Description 246
Firmware Updates 247
Raw Data Conversion to Absorbance 248
Rawdata File 251
Peak Detector 251
Spectra Acquisition Modes (DAD only) 252
Optional Interface Boards 253
BCD Board 253
LAN Communication Interface Board 255
Interfaces 256
Analog Signal Output 257
GPIB Interface 257
CAN Interface 257
Remote Interface 257
RS-232C 259
Setting the 8-bit Configuration Switch 261
GPIB Default Addresses 262
Communication Settings for RS-232C Communication 263
Forced Cold Start Settings 264
Stay-Resident Settings 265
The Main Power Supply Assembly 266
7 Control Module Screens for Agilent 1100 DAD and MWD
Major keys on the Agilent 1100 Control Module 270
Control Module - Unsupported Module 271
Screens available from the Analysis screen 272

1100 Series DAD and MWD Reference Manual 11
Screens available from the System screen 282
Screens available from the Records screen 284
Diagnostics and Tests 290
8 Specifications
Performance Specifications 296
A Safety Information
General Safety Information 300
Lithium Batteries Information 303
Disposal of Mercury from Deuterium Lamp 304
Radio Interference 305
Sound Emission 306
UV-Radiation 307
Solvent Information 308
Declaration of Conformity for HOX2 Filter 310
Agilent Technologies on Internet 311
Index

12 1100 Series DAD and MWD Reference Manual

13
Agilent 1100 Series Diode Array and Multiple Wavelength Detectors
Reference Manual
Agilent Technologies
1
Installing the Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Optimizing the Stack Configuration 20
Installing the Detector 22
Flow Connections to the Detector 25

14 1100 Series DAD and MWD Reference Manual
1 Installing the Detector
Site Requirements
A suitable environment is important to ensure optimal performance of the
detector.
Power Consideration
The detector power supply has wide ranging capabilities and accepts any line
voltage in the range mentioned in Table 1. Consequently, there is no voltage
selector in the rear of the detector. There are also no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply.
Power Cords
Different power cords are offered as options with the detector. The female end
of all power cords is identical. It plugs into the power-input socket at the rear
of the detector. The male end of each power cord is different and designed to
match the wall socket of a particular country or region.
WARNING To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the power switch on the front panel is turned off.
WARNING Shock hazard or damage of your instrumentation can result, if the devices are
connected to a line voltage higher than specified.
CAUTION Make sure to have easy access to the power cable of the instrument, in order to
disconnect the instrument from line.

1100 Series DAD and MWD Reference Manual 15
Installing the Detector 1
Bench Space
The detector dimensions and weight (see Table 1) allows you to place the
detector on almost any desk or laboratory bench. It needs an additional 2.5 cm
(1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the
rear for air circulation and electric connections.
If the bench should carry a Agilent 1100 Series system, make sure that the
bench is designed to bear the weight of all modules.
The detector should be operated in a horizontal position.
Environment
Your detector will work within the specifications at ambient temperatures and
relative humidity described in Table 1.
ASTM drift tests require a temperature change below 2 °C/hour (3.6 °F/hour)
over one hour period. Our published drift specification (refer also to
“Performance Specifications” on page 296) is based on these conditions.
Larger ambient temperature changes will result in larger drift.
Better drift performance depends on better control of the temperature
fluctuations. To realize the highest performance, minimize the frequency and
the amplitude of the temperature changes to below 1 °C/hour (1.8 °F/hour).
Turbulences around one minute or less can be ignored.
WARNING Never operate your instrumentation from a power outlet that has no ground
connection. Never use a power cord other than the Agilent Technologies power cord
designed for your region.
WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
CAUTION Do not store, ship or use your detector under conditions where temperature
fluctuations could cause condensation within the detector. Condensation will damage
the system electronics. If your detector was shipped in cold weather, leave it in its box
and allow it to warm up slowly to room temperature to avoid condensation.

16 1100 Series DAD and MWD Reference Manual
1 Installing the Detector
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 11.5 kg (26 lbs)
Dimensions
(width × depth × height)
345 × 435 × 140 mm
(13.5 × 17 × 5.5 inches)
Line voltage 100 – 120 or 220 – 240 VAC, ± 10 % Wide-ranging capability
Line frequency 50 or 60 Hz ±5%
Power consumption (G1315/65B) 300 VA / 125 W / 427 BTU Maximum
Ambient operating temperature 0 – 55 °C (32 – 131 °F) .
Ambient non-operating temperature -40 – 70 °C (-4 – 158 °F)
Humidity < 95%, at 25 – 40 °C (77 – 104 °F) Non-condensing
Operating altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the detector
Safety standards: IEC, CSA, UL, EN Installation category II, pollution degree 2

1100 Series DAD and MWD Reference Manual 17
Installing the Detector 1
Unpacking the Detector
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the detector may have been damaged during shipment.
Delivery Checklist
Ensure all parts and materials have been delivered with the detector. The
delivery checklist is shown below. Please report missing or damaged parts to
your local Agilent Technologies sales and service office.
CAUTION If there are signs of damage, please do not attempt to install the detector.
Table 2 Detector Checklist
Description Quantity
Detector 1
Power cable 1
UV-lamp (Part number 5181-1530) 1
CAN cable (Part number 5181-1516) 1
Flow cell As ordered
Reference Manual 1
Accessory kit (see Table 3) 1

18 1100 Series DAD and MWD Reference Manual
1 Installing the Detector
Detector Accessory Kit Contents
Table 3 Accessory Kit Contents (Part Number G1315-68705)
Description Part Number Quantity
Teflon Tubing flexible i.d. 0.8 mm (flow cell to waste),
re-order 5 m
5062-2462 2 m
Corrugated tubing (to waste), re-order 5 m 5062-2463 1.2 m
Fitting male PEEK 0100-1516 2
Capillary column-detector
380 mm lg, 0.17 mm i.d. includes:
G1315-87311 1
Ferrule front SST 0100-0043 2
Ferrule back SST 0100-0044 2
Fitting SST 79814-22406 2
Hex key set 1 – 5 mm 8710-0641 1
Wrench open end 1/4 – 5/16 inch 8710-0510 1
Wrench open end 4 mm 8710-1534 1
ESD wrist strap 9300-1408 1
Figure 1 Waste Tubing Parts
Fitting male
PEEK
Tubing

1100 Series DAD and MWD Reference Manual 19
Installing the Detector 1
Figure 2 Inlet Capillary (Column-Detector) Parts
Ferrule front
This side is
preinstalled
Ferrule back
Fitting male SST
Capillary

20 1100 Series DAD and MWD Reference Manual
1 Installing the Detector
Optimizing the Stack Configuration
If your detector is part of a complete Agilent 1100 Series system, you can ensure
optimum performance by installing the following configuration. This configuration
optimizes the system flow path, ensuring minimum delay volume.
Figure 3 Recommended Stack Configuration (Front View)
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Autosampler
Detector
Control module