Agilent Technologies 1100 Series User manual

A
Agilent 1100 Series
Diode Array and
Multiple Wavelength
Detectors
Reference Manual

Notices
© Agilent Technologies, Inc. 1995, 1996-
2004
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translation into a foreign language)
without prior agreement and written
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international copyright laws.
Manual Part Number
G1315-90005
Edition
Edition 05/2004
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Software Revision
This guide is valid for A.01.xx revisions of
the Agilent 1100 Series Diode Array and
Multiple Wavelength Detectors software,
where xx refers to minor revisions of the
software that do not affect the technical
accuracy of this guide.
2
Warranty
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douet is proided as is, ad is
subject to being changed, without
notice, in future editions. Further, to
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in damage to the product or
loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated
conditions are fully understood
and met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING
notice until the indicated
conditions are fully understood
and met.
FAR 12.211 (Technical Data) and FAR 12.212
(Computer Software) and, for Department of
Defense purchases, DFARS 252.227-7015

1100 Series DAD and MWD Reference Manual 3
(Technical Data - Commercial Items) and
DFARS 227.7202-3 (Rights in Commercial
C
o
m
p
u
t
er Software or Computer Software
Documentation). If a federal government
or other public sector Customer has a
need for
1100 Series DAD and MWD Reference Manual
I This Guide…
This manual contains information for using your Diode Array and
Multiple Wavelength Detectors.
1Installing the Detector
How to install the detector
2How to optmize the Detector
How to opimize the detector
3Troubleshooting and Test Functions
The detector’s built-in troubleshooting and test functions
4Repairing the Detector
Instructions on how to repair the detector
5Identifying Parts and Materials
Detailed illustrations and listings for parts and materials identification
for the detector
6Introduction to the Detector
An introduction to the detector, istrument overview, theory of
operation, external communication and internal connectors 7 Control
Module Screens for Agilent 1100 DAD and MWD

This chapter is intended to introduce an operator to the screens available
for operation of the Agilent 1100 diode array detector (DAD) with the
Agilent 1100 control module.
8 Specifications
Performance specifications of the detector
Annex A Safety Information
4 1100 Series DAD and MWD Reference Manual
Contents
1Installing the Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Damaged Packaging 17
Delivery Checklist 17
Optimizing the Stack Configuration 20
Installing the Detector 22
Flow Connections to the Detector 25
2How to optmize the Detector
Optimizing the Detector Performance 30
Optimization Overview 31
Optimizing for Sensitivity, Selectivity, Linearity and Dispersion
33
Flow Cell Path Length 33
Peak width (response time) 35

1100 Series DAD and MWD Reference Manual 5
Sample and Reference Wavelength and Bandwidth 36
Slit Width 39
Optimizing Spectral Acquisition (DAD only) 42
Margin for Negative Absorbance 43
Optimizing Selectivity 44
Quantifying Coeluting Peaks by Peak Suppression 44
Ratio Qualifiers for Selective Detection of Compound
Classes 46

6 1100 Series DAD and MWD Reference Manual
3 Troubleshooting and Test Functions
Oeie of the Deteto’s Idiatos ad Test Futios 50
Status Indicators 51
Power Supply Indicator 51
Detector Status Indicator 51
Error Messages 53
Timeout 54
Shutdown55
Remote Timeout 56
Synchronization Lost 57
Leak 58
Leak Sensor Open 60
Leak Sensor Short 61
Compensation Sensor
Open
62
Compensation Sensor
Short
Fan Failed 64
Open Cover 65
Cover Violation 66
Visible Lamp Current 67
Visible Lamp Voltage 68
Communication Error 69
63
Calibration Values Invalid 70
Diode Current Leakage 71
Holmium Oxide Test Failed 72

1100 Series DAD and MWD Reference Manual 7
UV Lamp Current 73
UV Lamp Voltage 74
UV Ignition Failed 75
UV Heater Current 76
New Messages with the G1315B and G1365B detectors
77 Module Type Changed 78
Illegal Value From Temperature Sensor Mounted On The
Fan
Assembly 80
Illegal Value Fo „Ai Ilet" Tepeatue Seso 81
Heater Failed 82
Heater Power At Limit 83
Wavelength Recalibration 84
Test Functions 85
Holmium Oxide Test 86
Intensity Test 88
Cell Test 90
Dark-Current Test 91
Filter Test 93
4 Repairing the Detector
Introduction into Repairing the Detector 96
Using the ESD Strap 98
Overview of the Repairing of the Detector 99
Simple Repairs 101
Exchanging a Lamp 102

8 1100 Series DAD and MWD Reference Manual
Exchanging a Flow Cell 105
Repairing the Standard or Semi-Micro Flow Cell 108
Repairing the High Pressure Flow Cell 112
Replacing Capillaries on a Standard Flow Cell 114
Replacing Capillaries on a Semi-Micro and High Pressure
Flow
Cell 119
Nano Flow Cell - Replacing or Cleaning 122
Cleaning or Exchanging the Holmium Oxide Filter
127
Correcting Leaks 130
Replacing Leak Handling System Parts 131
Cleaning or Replacing Coupling Lens Assembly
132 Cleaning or Replacing Cell Support Window
135
Exchanging Internal Parts 138
Removing the Top Cover and Foam 140
Exchanging the Detector Main Board 143
Entering the Serial Number using the Control Module
146
Entering the Serial Number using the
Agilent ChemStation 147
Exchanging the Fan (G1315A/G1365A) 148
Replacing Fan Parts (G1315B/G1365B) 150
Removing the Optical Unit (G1315A/G1365A) 154
Removing the Optical Unit (G1315B/G1365B) 156
Exchanging the Source Lens (Achromat) Assembly 158
Replacing the Holmium Oxide Filter Motor162

1100 Series DAD and MWD Reference Manual 9
Exchanging the Power Supply 164
Exchanging the Leak Sensor 167
Replacing Status Light Pipe 170
Installing the Optical Unit (G1315A/G1365A) 171
Installing the Optical Unit (G1315B/G1365B) 173
Replacing the Foam and the Top Cover 175
Assembling the Main Cover 178
Replacing the Interface Board 179
Replaig the Deteto’s Fiae 180
5 Identifying Parts and Materials
Overview of Main Assemblies 182
Optical Unit Assembly 184
Standard Flow Cell 186
Semi-Micro Flow Cell 188
High Pressure Flow Cell 190
Nano Flow Cells 192
Fan Assembly Parts 196
Holmium Oxide Filter197
Coupling Lens Assembly 198
Source Lens (Achromat) Assembly 199
Cell Support Assembly 200
Control Module 201
Sheet Metal Kit (G1315A/G1365A) 202

10 1100 Series DAD and MWD Reference Manual
Sheet Metal Kit (G1315B/G1365B) 203
Plastic Parts 204
Foam Parts 205
Power and Status Light Pipes 206
Leak Parts 207
Accessory Kit 208
Cable Overview 210
Analog Cables 212
Remote Cables 215
BCD Cables 220
Auxiliary Cable 222
CAN Cable 223
External Contact Cable 224
RS-232 Cable Kit 225
LAN Cables 226
6 Introduction to the Detector
Introduction to the Detector 228
Optical System Overview 229 Electrical
Connections 232
Instrument Layout 234
Early Maintenance Feedback (EMF) 235
EMF Counters 235
Using the EMF Counters235

1100 Series DAD and MWD Reference Manual 11
Electronics 237
Detector Main Board (DAM/MWM) 238
Firmware Description 246
Firmware Updates 247
Raw Data Conversion to Absorbance 248
Rawdata File 251
Peak Detector 251
Spectra Acquisition Modes (DAD only) 252
Optional Interface Boards 253
BCD Board 253
LAN Communication Interface Board 255
Interfaces 256
Analog Signal Output 257
GPIB Interface 257
CAN Interface 257
Remote Interface 257 RS-232C
259
Setting the 8-bit Configuration Switch 261
GPIB Default Addresses 262
Communication Settings for RS-232C Communication
263
Forced Cold Start Settings 264 Stay-Resident
Settings 265
The Main Power Supply Assembly 266

12 1100 Series DAD and MWD Reference Manual
7 Control Module Screens for Agilent 1100 DAD and MWD
Major keys on the Agilent 1100 Control Module 270
Control Module - Unsupported Module 271
Screens available from the Analysis screen 272
Screens available from the System screen
282
Screens available from the Records screen
Diagnostics and Tests 290
284
8
Specifications
Performance Specifications 296
A
Safety Information
General Safety Information 300
Lithium Batteries Information 303
Disposal of Mercury from Deuterium
Lamp
Radio Interference 305
Sound Emission 306
UV-Radiation 307
Solvent Information 308
304
Declaration of Conformity for HOX2 Filter
310
Agilent Technologies on Internet 311
Index

12 1100 Series DAD and MWD Reference Manual Agilent 1100 Series Diode Array and Multiple
Wavelength Detectors
Reference Manual
1
Installing the Detector
Site Requirements 14
Physical Specifications 16
Unpacking the Detector 17
Optimizing the Stack Configuration 20
Installing the Detector 22
Flow Connections to the Detector
25

1 Installing the Detector
14 1100 Series DAD and MWD Reference Manual
Agilent Technologies
Site Requirements
A suitable environment is important to ensure optimal performance of the detector.
Power Consideration
The detector power supply has wide ranging capabilities and accepts any line voltage in
the range mentioned in Table 1. Consequently, there is no voltage selector in the rear of
the detector. There are also no externally accessible fuses, because automatic electronic
fuses are implemented in the power supply.
To disconnect the detector from line, unplug the power cord. The power supply still
uses some power, even if the power switch on the front panel is turned off.
Shock hazard or damage of your instrumentation can result, if the devices are connected to a line
voltage higher than specified.
Make sure to have easy access to the power cable of the instrument, in order to disconnect the
instrument from line.
Power Cords
Different power cords are offered as options with the detector. The female end of all
power cords is identical. It plugs into the power-input socket at the rear of the detector.
The male end of each power cord is different and designed to match the wall socket of a
particular country or region.
WARNING
WARNING
CAUTION

Installing the Detector 1
1100 Series DAD and MWD Reference Manual 15
Never operate your instrumentation from a power outlet that has no ground connection. Never use
a power cord other than the Agilent Technologies power cord designed for your
region.
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
Bench Space
The detector dimensions and weight (see Table 1) allows you to place the detector on
almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space
on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and
electric connections.
If the bench should carry a Agilent 1100 Series system, make sure that the bench is
designed to bear the weight of all modules.
The detector should be operated in a horizontal position.
Environment
Your detector will work within the specifications at ambient temperatures and relative
humidity described in Table 1.
ASTM drift tests require a temperature change below 2 °C/hour (3.6 °F/hour) over one
hour period. Our published drift specification (refer also to “Performance
Specifications” on page 296) is based on these conditions. Larger ambient temperature
changes will result in larger drift.
Better drift performance depends on better control of the temperature fluctuations. To
realize the highest performance, minimize the frequency and the amplitude of the
temperature changes to below 1 °C/hour (1.8 °F/hour). Turbulences around one minute
or less can be ignored.
WARNING
WARNING
CAUTION

1 Installing the Detector
16 1100 Series DAD and MWD Reference Manual
Do not store, ship or use your detector under conditions where temperature fluctuations could cause
condensation within the detector. Condensation will damage the system electronics. If your detector
was shipped in cold weather, leave it in its box and allow it to warm up slowly to room temperature to
avoid condensation.
Physical Specifications
Table 1 Physical Specifications
Type
Specification
Comments
Weight
11.5 kg (26 lbs)
Dimensions
(width × depth × height)
345 × 435 × 140 mm
(13.5 × 17 × 5.5 inches)
Line voltage
100 –120 or 220 –240 VAC, ± 10 %
Wide-ranging capability
Line frequency
50 or 60 Hz ± 5 %
Power consumption (G1315/65B)
300 VA / 125 W / 427 BTU
Maximum
Ambient operating temperature
0 –55 °C (32 –131 °F)
.
Ambient non-operating temperature
-40 –70 °C (-4 –158 °F)
Humidity
< 95%, at 25 –40 °C (77 –104 °F)
Non-condensing
Operating altitude
Up to 2000 m (6500 ft)
Non-operating altitude
Up to 4600 m (14950 ft)
For storing the detector
Safety standards: IEC, CSA, UL, EN
Installation category II, pollution degree 2

Installing the Detector 1
1100 Series DAD and MWD Reference Manual 17
Unpacking the Detector
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your Agilent
Technologies sales and service office immediately. Inform your service representative
that the detector may have been damaged during shipment.
If there are signs of damage, please do not attempt to install the detector.
Delivery Checklist
Ensure all parts and materials have been delivered with the detector. The delivery
checklist is shown below. Please report missing or damaged parts to your local Agilent
Technologies sales and service office.
Table 2 Detector Checklist
Description
Quantity
Detector
1
Power cable
1
UV-lamp (Part number 5181-1530)
1
CAN cable (Part number 5181-1516)
1
Flow cell
As ordered
Reference Manual
1
CAUTION

1 Installing the Detector
18 1100 Series DAD and MWD Reference Manual
Accessory kit (see Table 3)
1
Detector Accessory Kit Contents
Table 3 Accessory Kit Contents (Part Number G1315-68705)
Description
Part Number
Quantity
Teflon Tubing flexible i.d. 0.8 mm (flow cell to waste),
re-order 5 m
5062-2462
2 m
Corrugated tubing (to waste), re-order 5 m
5062-2463
1.2 m
Fitting male PEEK
0100-1516
2
Capillary column-detector
380 mm lg, 0.17 mm i.d. includes:
G1315-87311
1
Ferrule front SST
0100-0043
2
Ferrule back SST
0100-0044
2
Fitting SST
79814-22406
2
Hex key set 1 –5 mm
8710-0641
1
Wrench open end 1/4 –5/16 inch
8710-0510
1
Wrench open end 4 mm
8710-1534
1
ESD wrist strap
9300-1408
1
Figure 1
Waste Tubing Parts
Fitting male
PEEK
Tubing

Installing the Detector 1
1100 Series DAD and MWD Reference Manual 19
Figure 2
Inlet Capillary (Col
umn-Detector) Parts
Ferrule front
This side is
preinstalled
Ferrule back
Fitting male SST
Capillary

1 Installing the Detector
20 1100 Series DAD and MWD Reference Manual
Optimizing the Stack Configuration
If your detector is part of a complete Agilent 1100 Series system, you can ensure
optimum performance by installing the following configuration. This configuration
optimizes the system flow path, ensuring minimum delay volume.
Figure 3
Recommended Stack Configuration (Front View)
Solvent cabinet
Vacuum degasser
Pump
Column compartment
Autosampler
Detector
Control module