Amersham AKTA UPC-900 User manual

User Manual
18-1125-55
Monitor UPC-900

Terms and Conditions of Sale
Unless otherwise agreed in writing, all goods and serv-
ices are sold subject to the terms and conditions of
sale of the company within the Amersham Biosciences
group which supplies them. A copy of these terms and
conditions is available on request.
Should you have any comments on this product, we
will be pleased to receive them at:
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Trademarks
Drop Design, ÄKTA, ÄKTAFPLC, and UNICORN are
trademarks of Amersham Biosciences Limited.
Amersham and Amersham Biosciences are trademarks
of Amersham plc.
Microsoft, Windows and Windows 2000 are either
registered trademarks or trademarks of Microsoft
Corporation in the United States and/or other coun-
tries.
Office Addresses
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Amersham Biosciences UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Biosciences Corp.
800 Centennial Avenue
P.O. Box 1327
Piscataway, N.J. 08855
USA
Amersham Biosciences Europe Gmbh
Munzinger Strasse 9
D-79111 Freiburg
Germany
Amersham Biosciences KK
Sanken Building
3-25-1 Hyakunincho, Shinjuku-ku
Tokyo 169–0073
Japan
© Copyright Amersham Biosciences AB 2002
- All rights reserved
Important user information
All users must read this entire manual to fully under-
stand the safe use of ÄKTA™monitor UPC-900
Workstation.
Safety symbols
The following Warning symbols highlights instruc-
tions that must be strictly followed in order to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated con-
ditions are met.
WARNING! Read the instruction to avoid
hazardous conditions.
WARNING! Avoid exposure to hazardous laser
radiation.
Caution notices
Caution! The Caution sign highlights instructions or
conditions that must be followed to avoid damage to
the product or other equipment. Be sure not to pro-
ceed until the instructions are clearly understood and
all stated conditions are met.
Notes
Note: The Note sign is used to indicate information
important for trouble-free and optimal use of the pro-
duct.
CE Certifying
This product meets all requirements of applicable CE-
directives. A copy of the corresponding Declaration of
Conformity is available on request.
The CE symbol and corresponding declaration of con-
formity, is valid for the instrument when it is:
– used as a stand-alone unit, or
– connected to other CE-marked Amersham
Biosciences instruments, or
– connected to other products recommended or
described in this manual, and
– used in the same state as it was delivered from
Amersham Biosciences except for
alterations described in this manual.
WARNING!
This is a Class A product. In a domestic environment
this product may cause radio interference in which
case the user may be required to take adequate
measures.


Contents
1 Introduction
1.1 General ..................................................................................................................1
1.2 Safety.....................................................................................................................2
2 Installation
2.1 Unpacking..............................................................................................................3
2.2 General precautions ...............................................................................................3
2.3 Installing the optical unit .......................................................................................4
2.4 Installing the conductivity flow cell .......................................................................6
2.5 Installing the pH flow cell and electrode................................................................6
2.6 Connecting electrical signal cables .........................................................................8
2.7 Connecting to a communication link .....................................................................8
2.8 Connecting to supply voltage.................................................................................9
2.9 Preparing the module for use .................................................................................9
2.10 Installing optical filters (optional)........................................................................10
3 Operation
3.1 On/off..................................................................................................................11
3.2 Menu selection and settings .................................................................................11
3.3 Main menu overview ...........................................................................................13
3.4 Controlling the UV lamp .....................................................................................13
3.5 Reading the UV absorbance value .......................................................................14
3.6 Autozeroing UV...................................................................................................14
3.7 Creating eventmarks ............................................................................................14
3.8 Setting Cond, pH and UV analogue outputs........................................................14
3.9 Calibrating conductivity ......................................................................................16
3.10 Calibrating the pH electrode................................................................................16
3.11 Filtering noise in the UV signal............................................................................19
3.12 Changing UV flow cell.........................................................................................19
3.13 Reading pH, temperature and conductivity values...............................................20
3.14 Storage and shut-down ........................................................................................20
3.15 Restart after power failure...................................................................................21
4 Maintenance
4.1 Periodic maintenance ...........................................................................................22
4.2 Cleaning and checking the module ......................................................................22
4.3 Cleaning the UV flow cell in-place.......................................................................23
4.4 Cleaning the UV flow cell off-line........................................................................23
4.5 Cleaning the conductivity flow cell in-place.........................................................24
4.6 Cleaning the conductivity flow cell off-line..........................................................24
4.7 Changing the conductivity flow cell.....................................................................24
Monitor UPC-900
User Manual 18-1125-55 Edition AE i
Contents

4.8 Cleaning the pH electrode ...................................................................................25
4.9 Changing the pH electrode ..................................................................................26
5 Trouble-shooting
5.1 General ................................................................................................................27
5.2 Faults and actions................................................................................................27
5.3 Error messages.....................................................................................................31
Reference information
A Description ..........................................................................................................34
A.1 Module .....................................................................................................34
A.2 UV optcal unit ..........................................................................................35
A.3 pH eletrode ...............................................................................................36
A.4 Condutivity cell.........................................................................................36
B Menu ...................................................................................................................37
B.1 Check menus.............................................................................................37
B.1.1 Checking autozero level............................................................37
B.1.2 Checking lamp run time ...........................................................37
B.1.3 Checking lamp intensity............................................................37
B.1.4 Checking recorder.....................................................................37
B.1.5 Checking service mode..............................................................37
B.2 Setup menus..............................................................................................38
B.2.1 Selecting setup sub menus.........................................................38
B.2.2 Setup adjust temperature ..........................................................38
B.2.3 Setup show temperature............................................................39
B.2.4 Setup conductivity temperature compensation..........................39
B.2.5 Setup conductivity reference temperature .................................39
B.2.6 Setup adjust conductivity..........................................................39
B.2.7 Setup adjust cell constant..........................................................41
B.2.8 Setup show conductivity ...........................................................41
B.2.9 Setup pH temperature compensation ........................................42
B.2.10 Setup show pH .........................................................................42
B.2.11 Setup UV averaging filter constant............................................42
B.2.12 Setup lamp run time .................................................................43
B.2.13 Setup show UV .........................................................................43
B.2.14 Setup language..........................................................................43
B.2.15 Setup unit number ....................................................................44
B.2.16 Setup display angle ...................................................................44
B.3 Setting and using the alarm timer..............................................................45
B.4 Service displays .........................................................................................45
B.5 Menu overview ....................................................................................46-47
C Technical specifications .......................................................................................48
C.1 Operating data..........................................................................................48
C.2 Physical data .............................................................................................49
D Accessories and Consumables ..............................................................................51
Monitor UPC-900
User Manual 18-1125-55 Edition AE
ii
Contents

Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1 – 5)
and a reference part (sections A – D).
Sections 1 – 5 contain the necessary information for operating the
instrument.
Monitor UPC-900
User Manual 18-1125-55 Edition AE iii
Contents

Monitor UPC-900
User Manual 18-1125-55 Edition AE
iv
Contents

1 Introduction
1.1 General
Monitor UPC-900 is a high precision on-line monitor for the
combined measurement of UV absorption, pH and conductivity in
liquid chromatography. The UPC-900 features:
• Fixed wavelengths of 254, 280 nm (Hg lamp), or 214 nm (Zn
lamp).
• Other wavelengths of 313, 365, 405, 436 and 546 nm through
filter change.
• Two alternative flow cells with pathlengths of 2 mm and 5 mm.
• Fast response
• High accuracy and reproducibility
• Flow cells with low dead volume
• Accurate and reliable monitoring through self-test and
self-calibration
• Flow cells can be connected close together, minimising band
broadening and time delay between detectors.
The monitor and the flow cells are separate units. All flow cells are
connected to the rear of Monitor UPC-900.
Monitor UPC-900
User Manual 18-1125-55 Edition AE 1
Introduction 1

1.2 Safety
• The module is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
• Operate in accordance with local safety instructions.
WARNING! Hg lamps contain small amounts of mercury and
must be handled with care and disposed of according to national
or local environmental regulations.
WARNING! HCl and NaOH are injurious to health. Avoid
spillage.
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
WARNING! The module uses high intensity ultra-violet light. Do
not remove the UV lamp while the monitor is running.
Before changing a UV lamp, ensure that the lamp cable is
disconnected from the monitor to prevent injury to eyes.
If the mercury lamp is broken, make sure that all mercury is
removed and disposed of according to national and local
WARNING! When lamp power is on, the lamp socket carries a
dangerous voltage. Do not connect/disconnect with the monitor
switched on.
WARNING! The module must not be opened by the user. It
contains high voltage circuits that can deliver a lethal electric
shock.
WARNING! Always disconnect the power supply before
attempting to replace any item on the module during
maintenance.
WARNING! The module must be connected to a grounded mains
socket.
Monitor UPC-900
User Manual 18-1125-55 Edition AE
2
1 Introduction

2 Installation
2.1 Unpacking
Unpack the module and check the items against the supplied packing
list. Inspect the items for obvious damage that may have occurred
during transportation.
Note: It is important that the filters, flow cells and lamps are not
handled during unpacking. For protection of these items, they should
remain in their packing materials until required for use.
We recommend that all packing materials be retained if onward
transport of the module is expected.
Note: pH measurement is optional in ÄKTAFPLC.
2.2 General precautions
The module should be located in a place of low temperature
variations, away from heat sources, draughts and direct sunlight.
The module may be operated at normal ambient temperatures in
the range +4 to 40 °C.
The module should be installed on a stable laboratory bench or
in ÄKTA FPLC™system rack. To ensure correct ventilation, a free
space of 0.1 m is required behind and in front of the module. Do not
place soft material under the module to ensure that the ventilation
inlet below the front is not blocked.
UPC-900
CAUTION! Read the following information carefully to ensure
that the module is installed correctly.
Monitor UPC-900
User Manual 18-1125-55 Edition AE 3
Installation 2

2.3 Installing the optical unit
Installing the holder
1 Hook the holder into the slot on the
right hand side of the module.
Secure it by pushing up the slide
clamp.
Flow cell installation
There is one analytical (5 mm) and one preparative (2 mm) flow cell
available (stand-alone units only include the preparative flow cell).
Both cells are installed in the same way, as described below:
1 Remove the red protective plugs from
the detector housing and the flow cell.
2 Insert the flow cell into the detector
housing from above.
Note: The flow cell can only be placed in
one correct position.
3 Secure the flow cell by turning the
locking nut until the stop position.
Note: If the locking nut is not tightened sufficiently, the monitor
will function poorly (e.g. drifting base-line).
4 Place the protective cover around the flow
cell to protect the electronics inside the
optical unit from liquid spillage.
Note: Avoid spillage for prolonged monitor
lifetime.
5 To remove the flow cell, reverse the
procedure.
Note: Ensure that the Hg lamp position and
the filter is selected according to the wave length to be used. This is
described in the Changing lamp assembly section below.
Connecting the optical unit to the module
1 Place the optical unit in the
holder, or in a suitable location
as close to the column as
possible. The optical unit can be
placed up to 1.5 m from the
monitor housing.
2 Secure it by tightening the screw in the holder.
Monitor UPC-900
User Manual 18-1125-55 Edition AE
4
2 Installation
Protective cover
for flow cell
Protective plug
for detector aperture
2 mm
5 mm
O-ring
Detector
housing
Locking
nut
Conductivity cell
Optical unit
UPC-900 Holder
Holder
Screw
Optical
unit

3 Connect the lamp cable to the socket Lamp on the rear panel of
the module.
4 Connect the signal cable to the socket Optical unit on the rear
panel of the module.
Changing lamp assembly
1 Use a Philips screwdriver to detach the end plate by removing
one and loosening the other of the two holding screws on the
lamp housing to be removed.
2 Slide the lamp housing off the filter housing.
3 Detach the end plate, as in step 1 above, from the lamp housing
to be fitted to the optical unit.
4 Slide the lamp housing onto the filter housing. The lamp and
signal cables should be on the same side. As you slide the lamp
housing into position, depress the two pressure pads on the
filter housing in sequence to facilitate the installation.
5 Refit the lamp housing end plate.
6 Slide the lamp housing firmly into place. There will be a faint
click when the housing is positioned correctly. The Hg lamp
housing can take up two positions, one for 280 nm, marked
by on the filter housing, and the other marked by for
all other wavelengths. The Zn lamp housing has only one
position.
7 Set the wavelength to be used by selecting lamp position
(indicated by a dot on the lamp housing) in combination with
the appropriate filter, i.e. the dot on the lamp housing should be
adjacent to the symbol on the filter housing corresponding to
the symbol on the filter wheel for the filter to be used. A click
will indicate that the filter is in position.
Note: The wavelength and the flow cell type should also be entered
in the Questions menu in UNICORN.
Filter change
The Hg optics with 254 and 280 nm filters and the Zn optics with
the 214 nm filter are delivered with filters installed. If other filters
are to be used, install the new filters as described in 2.10 Installing
optical filters (optional).
WARNING! The module uses high intensity ultra-violet light. Do
not remove the UV lamp while the monitor is running.
Before changing a defective lamp, ensure that the lamp cable is
disconnected from the monitor to prevent injury to eyes.
If the mercury lamp is broken, make sure that all mercury is
removed and disposed of according to national and local
environmental regulations.
Monitor UPC-900
User Manual 18-1125-55 Edition AE 5
Installation 2
Lamp housing end plate
Dot on lamp housing
Symbols on
filter housing
Symbol on
filter wheel
below lid

Monitor UPC-900
User Manual 18-1125-55 Edition AE
6
2 Installation
Connection to the column
1 Fix the optical unit directly under the column if possible.
Note: Always position the optical unit with the filter wheel cover
facing upwards.
2a Connect the column outlet tubing
directly onto the top (a) of the
optical unit for the 5 mm flow
cell using a fingertight connector.
2b Connect the column outlet tubing
onto the bottom (b) of the optical
unit for the 2 mm flow cell using
a fingertight connector.
Note: The inlet port of the 5 mm UV
cell is above the optical unit. The inlet
port of the 2 mm UV cell is below the
optical unit.
3 Screw to fingertightness.
4 Connect the optical unit outlet tubing onto the opposite hole in
the flow cell. Use fingertight connectors.
If no outlet tubing exists, cut a piece of PEEK tubing (i.d. 0.5
mm, o.d. 1/16”). The length should be 170 mm when using the
5 mm flow cell, and 230 mm when using the 2 mm flow cell.
5Connect the other end of the tubing to the conductivity flow cell
or to another appropriate unit.
2.4 Installing the conductivity flow cell
1 Place the conductivity cell in its cell holder, or
in a suitable location, as close to the optical
unit/column as possible. The cell can be
placed up to 1.5 m from the monitor housing.
Secure the cell with the clamp.
Note: When the conductivity flow cell is used
in conjunction with the pH electrode, place
the conductivity flow cell and select its flow
direction so that the screw head end of the flow cell faces the
pH flow cell.
2 Connect the conductivity cell to the socket Conductivity Flow Cell
on the rear panel of the module.
3 Connect the tubing with fingertight connectors.
Clamp
Holder
Conductivity
cell
b)
a)
2 mm
flow cell
5 mm
flow cell
Protective cover

2.5 Installing the pH flow cell and electrode
Mounting the flow cell holder
In ÄKTAFPLC systems, the pH electrode is optional.
1 Hook the flow cell holder on the right hand side of the housing.
Secure it with the slide clamp.
If the flow cell holder is not used, the flow cell must
still be installed at an angle of 30° from the vertical
with the outlet placed higher than the inlet to
prevent air bubbles being trapped in the cell.
The flow direction is marked on the flow cell.
2 Connect the tubing with finger-tight connectors.
Inserting the pH electrode
Note: Handle the pH electrode with care.
1 Unpack the pH electrode. Ensure that it is not broken or dry.
2 Before using the electrode, remove the electrode end cover and
immerse the glass bulb in buffer for 30 minutes.
3 Remove the dummy electrode from
the flow cell and store it in the
flow cell holder.
4 Carefully insert the electrode in the
flow cell. Tighten the nut by hand
to secure the electrode.
Note: If the flow cell is full of
liquid, it is not possible to insert
the electrode. If so, loosen the inlet
connection while inserting the
electrode to allow the liquid to run
out from the flow cell. Remember
to re-tighten the connector.
Note: If the electrode is not fully
inserted, the system will leak and a
dead volume will occur in the holder.
5 Connect the pH electrode cable to the socket pH Probe on the
rear of the module.
CAUTION! The tip of the pH electrode consists of a thin glass
membrane. Protect it from breakage, contamination and drying
out or the electrode will be destroyed. Always store the electrode
with the end cover filled with a 1:1 mixture of pH 4 buffer and
2 M KNO3. Do NOT store in water only.
Monitor UPC-900
User Manual 18-1125-55 Edition AE 7
Installation 2
End
cover
Electrode
To module
“pH probe”
socket
Flow direction
Flow cell
holder
Flow cell
Dummy

Monitor UPC-900
User Manual 18-1125-55 Edition AE
8
2 Installation
2.6 Connecting electrical signal cables
The sockets for electrical signals are located on the rear panel.
Connecting to chart recorder (if used)
The external chart recorder outputs for UV, pH and conductivity
from the monitor are 0–1 V.
1 Connect the chart recorder to the mini-DIN-socket Analogue
out 0-1 V using the cable supplied. Pin designations for the
signals are as follows:
Pin no. Signal Range Value (full scale)
1 UV 0-1 V 0.001-5 AU
2 signal ground 0 V -
3 conductivity 0-1 V 0.1 mS/cm-999.9 mS/cm
4 signal ground 0 V -
5 pH 0-1 V 0-14
6 signal ground 0 V -
Note: The signal cable is delivered with protective covers on each
wire. Do not remove the protective covers from unused connections
as a short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale.
2.7 Connecting to a communication link
The monitor can be used as a stand-alone module or in ÄKTAFPLC.
As a stand-alone module, it is controlled from the front dial and
display. In ÄKTAFPLC,the monitor is controlled from a PC running
UNICORN™version 3.0 or higher via UniNet 1 cables, or manually
from the front dial and display.
CAUTION! The mains power to ÄKTAFPLC must be switched
OFF before connecting the module to the UniNet 1 link.
1
2
3
4
5
6
Voltage Frequency Power, max
100-200 V- 50-60 Hz 30 VA
Conductivity
Flow Cell
pH-Ground pH-Probe
Optical
Unit
Analogue
out 0-1 V UniNet 1
Mains
Lamp

Connect two UniNet 1 cables to the UniNet 1 connectors. The
module can be connected in series anywhere between the PC and a
termination plug. The UniNet 1 link connects, in series, the PC with
Pump P-920, Monitor UPC-900 and the Frac-900. The termination
plug is connected to the last module in the chain.
2.8 Connecting to supply voltage
1 Make sure the on/off switch is in the OFF-position (0).
2 Connect the supplied mains cable between the module and a
grounded mains socket. Any voltage from 100–240 V AC,
50–60 Hz can be used.
Note: The module contains no user-replaceable fuse.
2.9 Preparing the module for use
Before performing the following procedures, we recommend you
read sections 3.1–3.3.
• Calibrate the pH electrode, see section 3.6.
• Set the conductivity cell constant, see section B.2.3.
(Only required when the flow cell has been replaced.)
• Calibrate the temperature sensor, see section B.2.6.
(Only required if the monitor is to be used for high accuracy
measurements, or if the flow cell has been replaced.)
Note: The conductivity cell constant is shown on the packaging.
Retain the packaging in case the conductivity cell constant needs to
be re-entered.
Note: The measured temperature is the temperature in the
conductivity flow cell, which can differ from the ambient
temperature in ÄKTAdesign systems.
Note: When running chromatography using organic solvents, we
recommend that the pH electrode is removed and the dummy
electrode inserted in its place as organic solvents will cause pH
electrode degeneration.
WARNING! The module must be connected to a grounded
mains socket.
Monitor UPC-900
User Manual 18-1125-55 Edition AE 9
Installation 2

Monitor UPC-900
User Manual 18-1125-55 Edition AE
10
2 Installation
2.10 Installing optical filters (optional)
The Hg optics with 254 and 280 nm filters and the Zn optics with
the 214 nm filter are delivered with filters installed. If other filters
are to be used, install them as follows:
1 If the Zn lamp is attached, remove
the lamp housing as described in section
Changing the UV lamp.
2 Remove the four screws in the filter
housing. Separate the filter housing from
the detector housing.
3 Carefully remove the filter wheel from the filter housing.
4 If necessary, remove the filter(s) from the filter wheel by
pressing it(them) out, e.g. with a small screwdriver.
Note: Filters are sensitive optical components. Never touch the
optical surfaces or expose them to temperatures above 60 oC.
Clean them with dry lens cleaning tissue and store them, when
not in use, in the box in which they were supplied. Heavy
contamination may be removed by using a lens tissue dipped in
ethanol.
5 Insert the filter(s) of choice into the filter wheel (maximum 3
filters) with the correct orientation (the mirror side facing
upwards) and position over one of the three triangular
apertures. The filters snap in by pressing them quite firmly. Do
not touch the filter surface.
6 Remove the circular plastic band showing the wavelength(s).
7 Remove labels from the band if necessary.
8 Place the correct labels on the band with the label designation
facing outwards. Ensure that the label position corresponds to
the filter position, i.e. the label should be placed opposite the
filter.
9 Reassemble the circular plastic band with the filter wheel peg
fitting into the band notch.
10 Check that all filters are clean. Insert the filter wheel back into
the filter housing.
Note: The filter wheel can be placed only in correct position.
11 Reassemble the filter housing with the detector housing by
fastening the four screws.
o214
o254
o280
o313
o365
o405
o436
o546
o214
o254
o280
o313
o365
o405
o436
o546
280
o
Triangular
aperture

3 Operation
3.1 On/off
Switch on the module at the mains switch on the rear panel. At
switch-on, the module performs a selftest. Several beeps are heard
during this process. If an error is detected, an error message is
shown.
Name and software version number is shown for 2 seconds.
All parameters are set to factory default values.
The selftest takes approx. 1 minute. When start-up is completed
with no errors, the display shows the main menu 1.
The monitor can be used immediately but the full specifications are
not obtained until after 1 hour of lamp warm–up.
3.2 Menu selection and settings
Menu selection
A specific menu is selected by turning the front selection dial
clockwise or counter-clockwise. When the required menu is visible,
the menu or selection is accepted by pressing the OK-button.
Menu selection OK-button ESC-button
Go down one menu Return one menu
level, or accept setting level
Select sub-menu Return one menu level
If a menu has sub levels, the sub menu is displayed by pressing OK.
Pressing ESC moves back one menu level.
AU Cond% pH
Calibrating
UPC-900
<version no.>
Selftest
Please wait...
Calibration ok
Monitor UPC-900
User Manual 18-1125-55 Edition AE 11
Operation 3

Return to main menu
Pressing ESC repeatedly always returns to the main menu 1, which is
the main operating menu.
Select value
A cursor below a text or numerical value shows what is affected by
the dial. To increase a numerical value, turn the dial clockwise. To
decrease the value, turn the dial counter-clockwise.
To simplify entering large numerical values, the cursor moves to next
digit if the dial is turned quickly in one direction. The cursor moves
back one place to the right every two seconds if the dial is not
turned. The text or numerical value displayed is accepted by pressing
OK. To cancel, press ESC.
Main menu 1
Main menu 2 ESC ESC ESC ESC
ESC
Main menu 1
Main menu 2
Main menus Sub menus
Sub menu 2.3.1
Sub menu 2.3.2
Submenu 2.1
Sub menu 2.2
Sub menu 2.3
Sub menu 2.1.1
ESC
OK
ESC
OK
ESC
OK
ESC or 3 click
CCW with the dial
3 click CW
with the dial
Monitor UPC-900
User Manual 18-1125-55 Edition AE
12
3 Operation
Setup Cond Ref Temp
Parameter
Current value New value to be set

3.3 Main menu overview
Main menu 1. This menu is accessed from all positions by
pressing ESC.
Lamp control. This menu is accessed by turning the dial one step
counter-clockwise from main menu 1. It is used to manually switch
the lamp on/off.
Autozero. Used to zero the UV signal from the monitor.
Eventmark. Used to set eventmarks on the UV curve.
Main menu 2. Used to display pH, temperature (in the conductivity
flow cell) and conductivity in mS/cm as well as in percentage of full
range.
Parameter menus. Used to set measurement parameters for Cond, pH
and UV.
Check menus. Checking internal operating values.
See Reference information section B.1.
Setup menus. Setting up language, unit number, etc.
See Reference information section B.2.
Alarm/Timer menus. Setting different timer options.
See Reference information section B.3.
3.4 Controlling the UV lamp
The Lamp menu is accessed by turning the dial one step
counter-clockwise from main menu 1, and then pressing OK. We
recommend the lamp be switched off to conserve lamp operating
time when no measurement is being made. A warm-up time of 60
minutes is required to achieve full specifications. However, in most
cases, a warm-up time of 15 minutes is sufficient.
Switching the lamp on/off
1 Switch the lamp on/off by moving the cursor with the dial, and
then pressing OK.
Lamp
(on) on off
Lamp
(on)
Alarm/Timer 00:22:24
Setup
Check
Set Parameters
pH 12.50 22.4
Eventmark
Autozero UV
Lamp
(on)
AU Cond%Tc pH
Monitor UPC-900
User Manual 18-1125-55 Edition AE 13
Operation 3
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