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Apostle MagTouch 1000 User manual

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Apostle MagTouch
1000
Nucleic Acids Extraction Automation System
USER MANUAL
Fast and
efficient
Apostle Inc is a biotechnology company located in San Jose, CA, providing cutting-
edge technologies and services for public health and life sciences. Apostle develops
innovative technologies in the space of liquid biopsy - the sampling and analysis of
non-solid biological tissue, primarily blood, often utilizing circulating free DNA
(cfDNA) as a biomarker. Apostle’s innovations include Apostle MiniMax, a new
scalable and automatable method to efficiently capture cfDNA from a standard
blood draw; Apostle MagTouch, a nucleic acids isolation automation system, and
Apostle MiniEnrich, a high-resolution DNA size enrichment technology using a
magnetic nano-platform. Apostle responds to the COVID-19 pandemic to help our
community fight together with a high quality, low cost, fast, automated, Apostle
COVID-19 Viral RNA Isolation System that has been clinically validated and has
processed over 1 million clinical samples. Apostle is a Stanford University StartX
Company, and a 2018 BioSpace Top 20 Life Sciences Startup Company.
Contents
Who We Are
03
Installation
04
Operation
1 1
Reagent Prep
13
Maintenance
15
Safety Guidelines
Contact Us
Phone: 1-888-992-7678
Email: info@apostlebio.com
www.apostlebio.com
16
Troubleshooting
4.
Use a 2.5 hex screwdriver to remove the
transportation fixing screw.
Keep the
transportation fixing screw.
(Figure 3)
03
3. The chamber of the MagTouch can be accessed
by sliding up the right-side window. Turn the knob
on the bottom right of the window to stabilize the
window in place.
(Figure 2)
Who We Are
Installation
1. Select an area for your MagTouch that is clean,
stable and has an electrical outlet nearby. Remove
your MagTouch and power cord from the shipping
box and place on the designated area.
2. Remove and keep all the tapes and foam
cushions used for transportation.
(Figure 1)
Do
NOT
connect power before removing the
transportation fixing screw.
5.
After removing the transportation fixing screw,
connect the power cord to the electricity and
turn on the machine.
6.
Once powered on, the Apostle MagTouch
system will automatically go through the
initialization process and examine the hardware
and software. Wait for the machine to pass the
initialization self-test. If any part of the self-test
fails, fix it according to the information shown
on the screen.
(Figure 4)
Figure 1
Figure 2
Figure 3
Figure 4
>
>
>
>
Who We Are
1. Before each run, fill in the Log book with
information including sample plate ID, time of
sample plate received, time of machine run and
machine number.
2. Get the prepared plates in hand and be ready
to start the program. (Sample Plate, two 80%
EtOH Plates, Elution Plate, Empty Plate with
Comb)
10. Make sure all the wells of Sample Plate (also
referred to as Lysis/Binding plate) are wide open
and no aluminum foil pieces are stuck out. If not
wide open, use pipette tip to open the wells.
(Figure 4)
[refer to operation checkpoint 1]
3. Press
[Initialization]
button on the top left
corner to initialize the machine.
5. Wait for the machine to pass the initialization
self-test. If any part fails, contact the lab director.
Operation
4. Press
[YES]
button to perform the initialization.
6. Press the
[Start]
button for
[viral RNA 2.0]
program on the screen.
(Figure 1)
7. Press the next step button on the top right
corner.
>
8. Open the plate-loading window gently.
(Figure
2)
Figure 2
Figure 3
Figure 4
9. Press the
[Plate position 1]
button on the
screen.
(Figure 3)
Figure 1
>
04
>
>
Who We Are
13. Take out two Apostle EtOH plates.
11. Place the Sample Plate on the
plate holder 1
in
the instrument through the plate-loading window.
Make sure that the plate is placed steadily in the
correct orientation. Ensure that the A1 well is in
the upper right corner of the plate holder on
turntable.
(Figure 5)
12. Press the
[Plate position 2]
button on the
screen.
15. Place one Apostle EtOH Plate on
plate holder
2
in the instrument through the plate-loading
window. Ensure A1 well is in the upper right corner.
14. Peel off the protection film on one Apostle
EtOH Plate.
(Figure 6)
16. Press the
[Plate position 3]
button on the
screen.
17. Peel off the protection film on the other
Apostle EtOH Plate.
Figure 5
18. Place the Apostle EtOH Plate on
plate holder
3
in the instrument through the plate-loading
window. Ensure A1 well is in the upper right corner.
Figure 7
19. Press the
[Plate position 4]
button on the
screen.
05
>
Figure 6
>
20. Take out the Apostle Elution Plate.
21. Stick the sample barcode onto the Elution
Plate.
(Figure 7)
>
29. Press the next step button on the top right
corner.
Figure 8
Figure 11
06
>
Figure 9
>
>
>
22. Peel off the protection film on the Apostle
Elution Plate.
23. Place the Apostle Elution Plate on
plate
holder 4
in the instrument through the plate-
loading window. Ensure A1 well is in the upper
right corner.
24. Press the
[Plate position 5]
button on the
screen.
25. Take out one Apostle Comb and an empty
plate. Don't touch the bottom columns of the
comb, grab the edge of the comb.
(Figure 8)
26. Place the comb into the empty plate carefully.
Ensure the comb is fully incorporated into the
plate.
(Figure 9)
27. Place the comb and empty plate on the
plate
holder 5
in the instrument through the plate-
loading window. Ensure A1 well is in the upper
right corner.
28. Close the plate-loading window gently.
30. Press the
[YES]
button to start the program.
31. After starting the program, check if the comb
gripper successfully grips the comb during [Grip
Comb] process on
position 5
. If gripping fails,
press the pause button on the screen and
immediately contact the lab director for hardware
adjustment.
(Figure 10)
[refer to operation
checkpoint 2]
32. Once the turntable moves to
position 1
,
observe if the comb smoothly inserts into the
plates and mixes the solution without moving
together with the plate.
(Figure 11)
[refer to
operation checkpoint 2]
Figure 10
35. Press the next step button on the top right
corner.
47. Press the next button on the top right
corner. The instrument will go back to the initial
interface and is ready for the next run.
Figure 12
Figure 15
07
>
Figure 13
>
>
>
Figure 14
33. If the program begins successfully, let the
instrument run the remaining steps. It will take
approximately
40 minutes
to finish the whole
program. If any issues, refer to the "Operation
Checkpoints" section of this manual.
34. After approximately 40 minutes, the program
will end and display
[Running status: Finish]
on
the screen and play a prompt tone.
36. Press the
[Plate position 4]
button on the
screen. (
Figure 12)
37. Open the plate-loading window gently.
38. Take out the Elution Plate from the plate-
loading window.
39. Seal the Elution Plate with protection film.
(Figure 13)
40. If you forget to stick the sample barcode on
the Elution Plate before running the program, stick
the sample barcode on now.
41. Check the Elution Plate with its sample
information sheet.
42. Place the Elution Plate together with its
sample information sheet into the
4 C
refrigerator.
(Figure 14)
43. Go back to the instrument and press the
[Plate position 1]
button on the screen.
44. Take out the Sample Plate from the plate-
loading window.
(Figure 15)
45. Repeat step 43-44 to take out all other plates
and comb from the instrument.
46. Dump all the used plates and comb into the
biohazard trash bin.
o
Checkpoint
Operation Checkpoints
When loading sample
plate, examine if all the
wells are wide open and
no aluminum foil pieces
are stuck out.
If it is not
wide open as shown
(Figure 1)
, use pipette tip
to make sure the wells are
wide open.
08
1
Figure 1
Figure 2
Checkpoint 2
After the start of the
program, check if the comb
gripper
successfully grips
comb
during [Grip Comb]
process on position 6.
(Figure 3)
If the gripping
fails, please pause the
program and immediately
contact lab director for
hardware adjustment.
Figure 3
Checkpoint 3 Once the plate is in the lifted position,
open the window and move the plate
forward, backward, left and right, so that
the comb can make the wells open. Then,
carefully separate the plate from the comb
and place the plate back to position 1
(Figure 6)
. Close the window, and resume
the program. If the plate is still gripped by
the comb and lifted from the turntable,
repeat this adjustment process. Note, if
the comb and plate are stuck together and
not lifted, manual movement of the plate
will be limited and does not open up the
wells.
Only adjust the plate when it is
lifted up from the turntable. Otherwise
the turntable may be damaged.
Operation Checkpoints
Once the turntable moves to
position 1, observe if the comb
smoothly inserts into the plates
and
mixes the solution without
moving together with the plates
as shown in
(Figure 4)
. If the
comb and the plate move together
(plate lifted up by the comb),
during [Binding] process when the
plate is gripped by the comb and
lifted from the turntable (usually
within 2 minutes into the program)
as shown in
(Figure 5)
, pause the
program.
09
Figure 4 Figure 5
Figure 6
Operation Checkpoints
10
If
Checkpoints 1-3
are missed, it may lead to a situation where the
comb grips the plate during [Binding] process and moves together to
position 2. If this happens, immediately turn off the MagTouch 1000
and contact the lab director.
Open the window and take out plates on position 3-6.
Lift the comb gripper and lysis/binding plate manually to give
space for wash plate, then take out wash plate on position 2.
Hold the lysis/binding plate and manually open the gripper to
release the comb and plate from the gripper.
Take out the lysis/binding plate and comb, then move the plate
forward, backward, to the left and right, so that the comb can
make the wells wide open.
Move the comb up and down several times and gently mix with the
solution in the sample plate to resuspend the beads back into the
solution. Then, carefully discard the comb without cross-
contaminating the wells.
Close the window and turn on the MagTouch, and check if the
initialization has passed successfully. If not, contact the lab
director immediately.
After initialization has completed, restart the program using the
retrieved plates and a new comb.
Make a note on the worksheet to notify other parties in case re-run
is needed.
To rescue the samples in this situation:
1.
2.
3.
4.
5.
6.
7.
8.