Appleton appZAP User manual

appZAP
UV Pipette Carousel
Instruction Manual
Distributed by
Appleton Woods Limited
New Lindon House
Catesby Park, Kings Norton
Birmingham B38 8SE
United Kingdom
Sales & Technical Support
Telephone: [phone]
[e-mail]
appleton-scientific.com
Product Diagram
Figure 1
Correct positioning
of pipettes
Knob for rotating
carousel
Start button Decontamination
chamber

Introduction
Thank you for your purchase of the appZAP UV Pipette
Carousel. The appZAP is designed to protect sensitive
assays and cell lines from contamination caused by carry
over on pipette shafts. The 254nm germicidal lamp in
the base of the appZAP is reflected by a highly mirrored
surface to expose the bottom part of the pipette shaft
to 360º of UV light. In one 28 minute exposure, UV light
breaks the bonds in DNA, causing thymine dimers to form.
This results in microorganisms being unable to grow or
replicate, and, ultimately, in cell death.
Before operating the appZAP Pipette Carousel, please
read this instruction manual carefully.
Technical Data
Capacity: 6 single channel pipettes
Lamp: 25nm, UV-C, non-ozone, 450µw
Lamp Life: Approximately 2,000 hours
Cycle Time: 28 minutes
Housing: UV-resistant polycarbonate
Reflector: Mirrored polyester film
Dimensions: 17cm diameter, 33cm height
Electrical: 100-240V, 50/60 Hz, 240W, 1A
Warranty: 2 years
Setting up the appZAP
Carefully remove the appZAP Pipette Carousel from the
packaging then place on a flat, stable surface away from
direct sunlight, water sources and heat sources. Keep the
packaging until the unit is shown to be in proper working
order.
Ensure the proper power cord has been received for the
local power supply. Plug the power cord into the back of
the appZAP and then plug the other end into a properly
grounded wall socket.
The appZAP is now ready for use.
Operation
The appZAP Pipette Carousel will accommodate 6 single
pipettes in the universal holsters. Pipettes should be
placed in the carousel with the tip ejector to the outside as
shown in Figure 1.
Note: Use with UV resistant pipette brands only,
e.g appPETTE, Gilson®, HTL®(the shafts on some
inexpensive pipettes will yellow and become brittle
over time from UV exposure). If in doubt, check with
your pipette’s manufacturer. Most reputable brands
are UV resistant.
Remove the pipette tip before placing the pipette into the
holster. For best results, the shaft of the pipette should be
wiped with ethanol and a soft cloth before placing in the
carousel. It is also recommended that steel tip ejectors be
occasionally autoclaved.
To begin the Auto-Decon™ cycle, press the “Start” button
on the base of the unit as show in Figure 2, below.
The Auto-Decon cycle will start immediately and take
28 minutes to
complete. The
chamber of the
appZAP will glow
slightly when the
UV light source
is activated.
Figure 2:
Position of Start button on the base
Once the cycle is complete, the pipettes can be removed
from the carousel and used immediately. There is no
waiting for pipettes to dry or cool and no recalibration is
necessary. Alternatively, pipettes may be stored in the
carousel for future use.
While the appZAP has been designed for user safety,
looking in to an empty pipette position may expose the
user to UV light. UV light can cause damage to the eyes
and should be avoided.
The carousel can be rotated for easier access to the pipettes.
Rotate the carousel by the knob at the top of the unit only.
Care and Maintenance
The exterior of the appZAP should be wiped down
periodically with a soft cloth. Do not immerse the unit in
water or place anything other than a pipette in the holsters.
Do not attempt to clean the mirrored surface. Should
cleaning be required, please contact Appleton Woods.
Take care not to let any particulate matter
enter the appZAP as this will reduce the
effectiveness of the germicidal lamp. Do not
place pipette tips in the appZAP.
If the appZAP will not be used for an
extended period of time, unplug the unit
and cover the carousel to prevent dust from
entering the UV chamber.
DO NOT ATTEMPT TO OPEN THE appZAP.
There are no user serviceable parts inside.
Opening the unit can result in injury and will
void the warranty. Touching the light source
can leave residues that will cause serious
damage to the light source.
The lamp in the appZAP contains mercury and must be
recycled. The lamp has an expected lifetime of 2,000
hours. Should replacement be required, please contact
Appleton Woods on 0121 458 7740 or email
[e-mail].
Warranty
AppZAP UV Pipette Carousel is covered
by the Appleton Promise which means
that should any faults arise within the
2 year warranty period it will be
replaced free of charge. Damage from
neglect, abuse, misuse or dropping the
unit will not be covered under the warranty.
Gilson and HTL and registered trademarks of their respective companies.
Never look down in to the pipette chamber
when the Auto-Decon cycle is running
promise