General Guidelines
There are four main stages to refreshing the column by replacing the wetted flowpath. The first three stages are based
on the disassembly and reassembling each column sub assembly with new components before the final stage of
rebuilding and testing the complete column.
STAGE 1 –Refresh the Adjuster Assembly of the Evolve®350 Process Column
STAGE 2 –Refresh the Tube Assembly of the Evolve®350 Process Column
STAGE 3 –Refresh the Fixed Assembly of the Evolve®4350 Process Column
STAGE 4 –Rebuild and Test the Evolve®350 Process Column
To disassemble and assemble the columns follow the instructions below. After reassembling the column ensure that the
column does not leak by performing a hydrostatic leakage test.
NOTES: When assembling/dissembling attention should be paid to:-
1. Always use the correct size tools. A comprehensive tool kit was provided with the original column. See Table
below.
2. Ideally components should be at approximately room temperature. If not, take particular care as thermal
expansion or contraction may make components fit more tightly and thus more difficult to disassemble.
3. Dismantled assemblies should be rested on a clean, dry surface.
4. Pay particular care not to damage the edge of the flowcells.
5. When unpacking columns be careful not to scratch the inside of the column tubes.
6. Be careful not to damage stainless steel surfaces by bumping, knocking or scratching.
7. Do not over-tighten components and use correct torque settings as given in this guide.
8. Good engineering practices should always be followed and operations carried out in an approved and safe manner.
9. The components of the Evolve®350 column are heavy and large. Mechanical handling equipment or manual
handling aids should be utilised to safely lift or move the Column or its subassemblies. Provision is made for the use
of a hoist as eye bolts are fitted or supplied. It is essential that ‘good practice’ is adopted regarding the use of
appropriate slings and associated equipment. See original column manual for guidance.
10. All these operations should be performed by two people.
