Astrea Evolve User manual

Evolve®350mm Column
Instructions for Replacing the Wetted
Flowpath using the Column Refresh Kit

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
2
Table of Contents
Replacing the Wetted Flowpath ........................................................................................ 3
General Guidelines ........................................................................................................ 4
STAGE 1 –Refresh the Adjuster Assembly ............................................................................ 6
Step 1-Disassembly of Adjuster Assembly............................................................................. 6
Step 2-Reassembly and Refresh the Adjuster Assembly ............................................................ 8
STAGE 2 –Refresh the Tube Unit Assembly..........................................................................10
Step 1-Disassembly of the Tube unit ................................................................................. 10
Step 2-Reassembly and Refresh the Tube unit ...................................................................... 12
STAGE 3 –Refresh the Fixed End Assembly..........................................................................13
Step 1-Disassembly of the Fixed End Assembly ..................................................................... 13
Step 2-Reassembly and Refresh of the Fixed Assembly ............................................................ 14
STAGE 4 - Column Reassembly and Preparation For Use..........................................................15
Step 1 –Assemble Tube Unit onto the Column base and Bottom flowcell. ..................................... 16
Preparing for Column Use ...............................................................................................18
Step 1 - Hydrostatic Leak Test ........................................................................................ 18
Step 2 - Levelling the 350mm Columns .............................................................................. 18

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Replacing the Wetted Flowpath
This manual describes the procedures necessary to replace the wetted flowpath components in the Evolve®350 column
using the Column Refresh Kit AA35CRK3.
Description
QTY
Flow Tube Adaptor 10mm Bore
1
Adjuster Flow Tube with seals 10mm Bore
1
Flow Tube Seal,10mm Bore
3
Bed Support retaining ring
1
Bed support retaining bolt
2
Adjuster Seal, 350mm Column
1
Adjuster bed support
1
Fixed bed support
1
Fixed bed support seal /Fixed Tube Seal
3
Fixed cell seal
1
Pipespool Elbow
1
Flow Tube Seal, Ladish 10mm Bore
3
Click Clamp
3
Blanking caps
2
Adjuster Cell 350 assembly including flowtube
adaptor, bolts and inserts and gasket
1
Fixed cell assembly including flowtube adaptor,
bolts, inserts and gasket
1
Fixed Flange
1
Locking Flowcell Nut
1
Tube, 350 X 530
1
Buffer Ring, 350
2
Scale Label, 350
1
Serial Number Label
1
Rating plate
1
Alignment Indicators
2
Evolve Label
1
Fitting Instructions
1
Figure 1 List of Refresh Kit components and Column
schematic. Parts shown in RED are the wetted
flowpath components.

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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General Guidelines
There are four main stages to refreshing the column by replacing the wetted flowpath. The first three stages are based
on the disassembly and reassembling each column sub assembly with new components before the final stage of
rebuilding and testing the complete column.
STAGE 1 –Refresh the Adjuster Assembly of the Evolve®350 Process Column
STAGE 2 –Refresh the Tube Assembly of the Evolve®350 Process Column
STAGE 3 –Refresh the Fixed Assembly of the Evolve®4350 Process Column
STAGE 4 –Rebuild and Test the Evolve®350 Process Column
To disassemble and assemble the columns follow the instructions below. After reassembling the column ensure that the
column does not leak by performing a hydrostatic leakage test.
NOTES: When assembling/dissembling attention should be paid to:-
1. Always use the correct size tools. A comprehensive tool kit was provided with the original column. See Table
below.
2. Ideally components should be at approximately room temperature. If not, take particular care as thermal
expansion or contraction may make components fit more tightly and thus more difficult to disassemble.
3. Dismantled assemblies should be rested on a clean, dry surface.
4. Pay particular care not to damage the edge of the flowcells.
5. When unpacking columns be careful not to scratch the inside of the column tubes.
6. Be careful not to damage stainless steel surfaces by bumping, knocking or scratching.
7. Do not over-tighten components and use correct torque settings as given in this guide.
8. Good engineering practices should always be followed and operations carried out in an approved and safe manner.
9. The components of the Evolve®350 column are heavy and large. Mechanical handling equipment or manual
handling aids should be utilised to safely lift or move the Column or its subassemblies. Provision is made for the use
of a hoist as eye bolts are fitted or supplied. It is essential that ‘good practice’ is adopted regarding the use of
appropriate slings and associated equipment. See original column manual for guidance.
10. All these operations should be performed by two people.
CAUTION!
THESE PROCEDURES MUST BE PERFORMED USING AN OVERHEAD HOIST

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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The following tool kit was provided with the original column.
Tool
Where used
Allen Key Set
Various locations
Torque Wrench and 14mm Socket
Tie Bar fixing Bolts
Spanner 17mm/13mm AF
Flowtube adaptor, Adjuster Flowtube, Column Feet
Spanner 19mm/24mm
Flanges bolts Adjuster, Fixed and locking flowcell nut
Spirit Level
Used to level the column
S/Driver with Wide Blade
Removal of bed support retaining ring
Eye Bolt
Additional eye bolts to fit to tube unit during
maintenance procedures
Alignment indicators are attached to the top flange, tube flanges, bottom flowcell and column base. These assist with
reassembly of the column and ensure correct orientation of the column components.
Figure 2 Photograph of Evolve®350 showing alignment indicators.
Alignment Indicators

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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STAGE 1 –Refresh the Adjuster Assembly
Step 1-Disassembly of Adjuster Assembly.
1. Detach any pipework from the top sanitary clamp adapter.
2. Remove flange fixing bolts from the top column tube flange. Retain bolts, nuts
and washers.
3. Check that adjuster seal is released by turning the seal actuation nut counter
clockwise.
4. If necessary, remove the handles from the seal actuation nut if they interfere
with the hoist slings.
5. Using a hoist lift adjuster unit out of tube unit.
6. Move the adjuster along the hoist rail or move the remaining column unit so
access to the lifted adjuster unit is possible.
7. Place the adjuster assembly so that the flowcell face is on the floor with the
hoist still attached.
Identify the parts as shown in the schematic and photograph below.
CAUTION!
Adjuster units are very heavy. Lifting should only be done using appropriate lifting aids.
Figure 3 Photograph of
Evolve®350 Adjuster unit
removal from column tube
Figure 4 Adjuster Assembly Evolve®350 column

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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a) b) c) d)
Figure 5 Disassembly stages of the Adjuster Assembly
8. Remove sanitary clamp adaptor using the 17mm spanner and flowtube seal.
9. Remove the seal actuation nut and spacer (see a) of Figure 5).
10. Slowly, using the hoist, lift the adjuster flange. This will cause the flowtube, seal setting tube and flowcell to slide
out of the threaded rod (see b) Figure 5).
11. Stand the flowcell on a flat surface (see c) Figure 5.
12. Using “flats” on undo the seal setting tube and slide it upwards and retain.
13. Using correct spanner to remove the flowtube and discard.
14. Discard the adjuster bed support, flowcell, bed support retaining ring and bed support retaining bolt (see d)
Figure 5).
15. Lower the remaining components of adjuster assembly onto a flat surface.
16. Disconnect the hoist.
17. Refer to the table below and ensure the correct components are retained and discarded.
ADJUSTER ASSEMBLY
Wetted Flowpath components
DISCARDED
Non-wetted components
RETAINED
Sanitary Clamp Adaptor
Seal Actuation Nut
Flowtube seals x2
Spacer
Flowtube
Stainless steel Seal Adjust tube
Adjuster bed support
Threaded central height adjust rod
Adjuster Flowcell assembly, inc flowcell inlet, flowcell
locking nut and gasket seal
Height adjust handle assembly
Adjuster seal
Adjuster Flange
Bed support retaining ring
Seal setting ring
Bed support retaining bolt
Backing Plate

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Step 2-Reassembly and Refresh the Adjuster Assembly.
The adjuster flowcell is supplied as an assembled unit which simplifies adjuster assembly protocols. Refer to
photographs below to provide visual guidance through the procedure.
a)
Figure 6 Assembly stages of the Adjuster Assembly
1. Place the adjuster flowcell on a flat surface (see a) Figure 6) and check the flowcell locking nut is located against
the flowcell (see b) Figure 6).
2. Locate the flowtube seal and fit the flowtube. Use the correctly sized spanner to tighten (see a) Figure 7).
a) b) c) d)
Figure 7 Assembly stages of the Adjuster Assembly –Connection to back of Adjuster Flowcell
3. Slide the stainless steel central seal setting tube over the flow tube and connect to the flowcell inlet ensure that
the tube is fully engaged and no manual rotation can be achieved (see b) Figure 7).
4. Move the flowcell locking nut upwards so it contacts the end of the seal setting tube (see c) Figure 7).
5. Tighten the seal setting tube against the locking nut (see d) Figure 7).
6. Fit the adjuster seal around the perimeter of the top flowcell.
7. Ensure eye bolts are fitted to the adjuster flange.
8. Using appropriate lifting slings attach the hoist to the adjuster assembly and lift the unit high enough to allow the
flowcell assembly to be fitted.
b)
CAUTION!
Do not over tighten as this may lead to failure of the component

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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a)
Figure 8 Reassembly of Adjuster Assembly
9. Move the adjuster assembly over the flowcell and lower so the flowcell assembly slides into the threaded height
adjust rod/seal setting ring assembly (see a) Figure 8) and ensure the adjuster seal is not trapped beneath the seal
setting ring (see b) Figure 8).
10. Locate the spacer and fit the seal actuation nut (see c) Figure 8).
11. Locate the flowtube seal and fit the sanitary clamp adapter.
12. The refresh of the adjuster assembly is now complete. Ensure the unit is firmly seated on the floor and detach the
hoist.
b)
c)
CAUTION!
Do not over tighten as this may lead to failure of the component

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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STAGE 2 –Refresh the Tube Unit
Assembly
Step 1-Disassembly of the Tube unit.
1. Attach the spare set of eye bolts provided with the original column, to the top tube flange.
2. Using appropriate lifting slings or equipment attach the hoist to the eye bolts.
3. Identify the components on the schematic shown in Figure 9.
Figure 9 Column Tube unit
TUBE UNIT ASSEMBLY
Wetted Flowpath components
DISCARDED
Non-wetted components
RETAINED
Tube (complete with scale label, Id labels)
Top column flange
Buffer seals
Tie bars and bolts
Bottom column flange
Bottom flange bolts, washers and nuts
4. Using the correct spanners, remove and retain the column tube bottom flange bolts, washers and bolts.
5. Lift up the column tube unit and when suspended loosen the tie bar fixing bolts that are located in the bottom
column flange.
6. Place the tube unit on a flat surface.

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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7. Remove and retain the tie bar fixing bolts from the top flange and remove the top flange.
Figure 11 Disassembly of the Column Tube Unit
8. Remove tie bars to allow the tube to be lifted out of the bottom tube flange. If tube is difficult to remove from
flange remove the lower buffer seal.
9. Discard the tube and buffer seals.
Figure 10 Removal of tube unit
from Column Base

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Step 2-Reassembly and Refresh the Tube unit.
In the column refresh kit, the tube unit is partially assembled and is supplied with scale label and buffer seals in
position.
1. Identify the replacement bottom tube flange. Turn and support on its side to allow the attachment of the tube
support rods using the correct bolts and allen key.
2. When all rods are located and fixed place the flange on a clean flat surface.
3. Locate the column tube seal into the groove in the flange.
4. Ensure the tube is orientated correctly, the orientation of the tube is shown by the fitted scale label.
5. The buffer seals should be in place on the outside diameter of the tube at a distance approximately 2cm from the
end of the tube.
6. Two Person Operation Locate the acrylic tube in the bottom column flange. Ensure it is located centrally and push
down to ensure correctly seated.
7. Lift the top column flange and place so that the tube support rod bolts can be engaged. Ensure alignment
indicators are aligned.
Figure 12 Reassembly of Column Tube Unit
8. Lower the top flange and secure the bolts by hand but do not tighten the bolts.
9. Locate the upper and lower buffer seals into the bottom flange and underside of the top column flange.
10. Using the correct Allen key tighten the tube support rod bolts located in the top column flange.
11. Use the hoist to lift the tube unit ready to assemble to column bottom fixed assembly.
12. Check the tube support rod bolts in the bottom and top flange and tighten if necessary. The torque wrench and a
setting of 50Nm is recommended.

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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STAGE 3 –Refresh the Fixed End
Assembly
Step 1-Disassembly of the Fixed End Assembly.
1. To assist it is recommended that column feet are lowered to provide a stable base.
2. Identify the components on the schematic shown in Figure 13.
Figure 13 Fixed Assembly Evolve®350 column
3. Using the correct size allen key, remove and retain the pipespool clamp top.
4. Remove the pipespool elbow from the flowcell assembly.
5. Undo, remove and retain bottom flowcell bolts.
6. Remove the bottom flowcell from the backing plate and discard.
ADJUSTER ASSEMBLY
Wetted Flowpath components
DISCARDED
Non-wetted components
RETAINED
Sanitary Clamp Adaptor
Bottom Cell fixing screw
Flowtube seal
Pipespool clamp and fixing bolts
Flowtube
Backing Plate
Bottom Bed Support assembly
Column Levelling feet
Bottom Flowcell
Casters
Pipespool elbow
Bottom Column Flange
Santoprene Ladish Seal

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Step 2-Reassembly and Refresh of the fixed assembly.
The fixed end flowcell is supplied complete with the process port in place.
1. Place the bottom flowcell on the backing plate using the alignment indicators to ensure flow cell is in the correct
orientation.
2. Secure the flow cell to the column base plate using the bottom flowcell bolts.
3. Locate the bed support seal into the inner most groove on the flowcell.
4. Locate the fixed flange seal into the outer most groove on the flowcell.
5. Locate the bed support with the smooth side uppermost and secure using the bed support retaining bolt. Do not
overtighten this bolt.
Note: Always use clean dry gloves when handling bed supports.
6. Connect the pipespool elbow to the flowcell and secure in place by refitting the pipespool clamp top.
Figure 14 Reassembly of Column Bottom Flowcell and Fixed end

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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STAGE 4 - Column Reassembly and
Preparation For Use
Note: Reassembly of the column is the reverse of disassembly and starts from column bottom upwards. Each sub
assembly is reassembled before attached to the column.
The column is supplied with alignment indicators , these are attached to the top flange, tube flanges, bottom flange
and base plate. These assist with the reassembly of the column and ensure correct orientation of components.
Figure 15 Alignment Indicators on Evolve® 350 column
Alignment Indicators

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Step 1 –Assemble Tube Unit onto the Column base and Bottom flowcell.
1. Attach appropriate lifting slings to the eye bolts located on the tube unit flange. Lift the tube unit.
2. Move the tube unit over the fixed end assembly.
Figure 16 Reassembly of Column - tube unit to fixed end
3. Check that the O-rings seal are in the correct positions.
4. Align the tube unit using the alignment indicators.
5. Replace the bottom column flange bolts/washers in the tube unit.
6. Slowly lower the tube unit and use the column bottom flange bolts to align the tube unit onto the bottom flange.
7. When tube unit is in place on column base, locate the column bottom flange nuts, initially, hand tighten, working
diametrically opposite to ensure equal loading on the seal. Then using the correct spanner working diametrically
opposite to tighten.
8. Remove the eye bolts from the top flange and store with other column tools.
9. Attach appropriate lifting slings to the eye bolts located on the adjuster assembly. Lift the adjuster assembly.

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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10. Move the assembly over the column unit. Use alignment indicators to line up the adjuster. Slowly, position the
adjuster unit into the column tube. Alignment pins located in the top flange are provided to assist positioning.
Figure 17 Location of Adjuster Assembly completing Column Refresh Procedure
11. Locate the top flange fixing bolts/washers, initially, hand tighten, working diametrically opposite to ensure equal
loading. Then using the correct spanner working diametrically opposite to tighten fully.

Evolve®350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit
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Preparing for Column Use
Step 1 - Hydrostatic Leak Test
The purpose of this test is to check that there are no leaks from the column and the column is ready to use.
1. Fill the column with WFI (water for injection) or high quality water and position the adjuster at the operating bed
height.
2. Ensure the adjuster seal is wetted with buffer or other suitable liquid prior to assembly. This helps ‘lubricate’ the
seal to ensure correct seating.
3. Activate the adjuster seal.
4. Ensure all air is removed from the column and any connections.
5. Attach a calibrated pressure gauge to the top inlet of the column.
6. Open the valve and increase the pressure until 2.0bar (29psi) and close bottom inlet valve. Wait 15mins for the
pressure to stabilize.
7. Release the pressure in the column.
8. Open the valve and increase the pressure until 4bar (58psi) and close bottom inlet valve. Wait 15mins for the
pressure to stabilize.
9. Check the pressure and ensure pressure is 4 bar (58psi).
Due the nature of materials it is possible there will be a small pressure drop, however, this should not exceed 0.2 bar.
Step 2 - Levelling the 350mm Columns
The 350mm columns are fitted with height adjustable feet and a spirit level is provided. This enables the column to be
leveled or to increase ground clearance.
1. Ensure the column is in the final process position and the column has been disconnected from any overhead hoist.
2. Place the spirit level on the top surface on the column.
3. Locate the flat portion on the column feet bolt and using the correct spanner adjust so that the bubble is located
centrally on the spirit level.
4. Turn the spirit level by 90 degrees and repeat.
CAUTION!
Due to the materials of fabrication, when the column is initially exposed to pressure the column components will
flex. It is important that the hydrostatic leak test is performed as outlined below following a multiple step operation.

Issue date: 22Oct2021
CCR number: CCR-1073
Rebranded by: T Hayward
Technically reviewed by: R Dodd
QA issued by: R Hawkins
The content in this document was provided by Delta Precision Ltd.
Evolve is a registered trademark of Delta Precision Ltd. Evolve is a registered trademark in the EU, UK and US.
All other trademarks, trade names, trade dress, product names and logos appearing on this document are the property of Astrea Bioseparations Ltd.