BAS C3 User manual

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C3
April, 2001
Part MF-9085 INSTRUCTION MANUAL
C3 Cell Stand
Bioanalytical
Systems, Inc.
2701 Kent Avenue
West Lafayette
Indiana 47906
www.bioanalytical.com
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Copyright 1997, 2001
Bioanalytical Systems, Inc.
www.bioanalytical.com
This instrument, either wholly or in part, is manufactured for research purposes only. Use for medical diagnosis is not intended,
implied, or recommended by the manufacturer. Use for this purpose and accountability for same rest entirely with the user.
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Table of Contents
Section 1. Introduction . . . ............................................ 1
Features . . ................................................... . 1
Specifications . . ................................................ 1
Section 2. General Information ......................................... 2
Technical Changes ............................................... 2
Shipping Damage . ............................................... 2
Limited Warranty . . .............................................. 2
Service....................................................... 3
Section 3. Installation . . ............................................. 4
Inspection of Shipment . . ......................................... 4
Parts of C3 Cell Stand ............................................ 4
Location of C3 Cell Stand ......................................... 4
Power Requirements ............................................. 5
Back Panel Controls and Connections ................................. 6
Connections ................................................... 7
Section 4. Operation ................................................ 12
Polarity of Remote Stir Control . . .................................... 12
Front Panel Controls and Connections . . . .............................. 12
Remote Control . . . .............................................. 14
Gas Purge ..................................................... 14
Stir.......................................................... 14
Faraday Cage . . ................................................. 14
Removal of Cell Lead Box . . ........................................ 14
Section 5. Maintenance .............................................. 15
General Maintenance ............................................. 15
Troubleshooting . . . .............................................. 15
Service Procedure ................................................ 15
Table of Contents
i
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Section 1. Introduction
Finite current electrochemistry is commonly used for analytical purposes. The BAS C3 Cell
Stand is specifically designed to conveniently use the variety of solid electrodes and cell
accessories available from BAS.
The C3 Cell Stand interfaces directly with epsilon, BAS 100 series Electrochemical
Analyzers, the CV-50W and other BAS Voltammographs. The cell can be enclosed in a
Faraday Cage to minimize electrical interference. A built-in gas control allows purging or
blanketing of the sample prior to or during analysis. The magnetic stirrer allows for
controlled agitation of the sample for experiments requiring convective mass transport of
analyte to the electrode surface. The cell arm with detachable cell top allows easy access to
the electrodes for rinsing, cleaning, or replacement. The stir motor/cell vial base pivots for
easy removal and replacement of the cell vial.
The C3 Cell Stand is part of an Electrochemical Accessories Package. The package also
includes voltammetry cells, reference electrodes (silver/silver chloride), working electrodes
(glassy carbon and platinum), an electrode polishing kit, and connecting tubing.
Features • Complete package
• “Quick-connect” input gas line connector
• Manual or remote control of gas purge/blanket
• Manual or remote on-off control of magnetic stirrer
• Manual 16-position adjustment of magnetic stirrer rate
• Small volume glass cell vials
• Mounted cell top compatible with all BAS solid working electrodes
and reference electrodes
• Cell lead connects directly to all BAS electrochemical controllers
(Voltammographs or Electrochemical Analyzers)
• Standard addition port
• Open architecture for easy access to cell
• Faraday Cage available for low current measurements
Specifications Power: 100-240 VAC, 50/60 Hz, V-A 10 Watts
Main Fuse: 2.0 A Slow Blow
Inlet Gas Pressure: 5 psi maximum
Size: 7.25" (18 cm) wide x 9.25" (23 cm) deep x 11.5" (29 cm) high
Weight: 10.5 lbs (5 kg)
Section 1. Introduction
1
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Section 2. General Information
User Updates To receive product update news and valuable information related to this and other BAS
products, fill out and return the BAS Warranty Enrollment Card shipped with your C3 Cell
Stand. We would like to know who you are and how we can meet your electrochemical
analysis needs.
Technical Changes BAS may make technical changes to improve the instrument. Improvements affecting use or
maintenance will be described in supplementary pages to this manual.
Shipping Damage Damage to any part of the C3 Cell Stand during shipping should be reported immediately to
the freight handler and BAS Customer Service. Unless other arrangements have been made,
the freight handler (shipper) is responsible for all damage or breakage to the instrument and
parts. Retain the original packing box and contents for inspection by the freight handler.
BAS will replace any new instrument damaged in shipping with an identical product as
quickly as possible after the claim filing date. Claims not filed within seven (7) days after
receipt of shipment may be invalid.
Do not return damaged goods to BAS without first contacting Customer Service for a Return
Authorization Number (RA#). When a defective part is returned to BAS, the RA# immediately
identifies you as the sender and describes the item being returned. BAS refuses all
unauthorized return shipments.
Limited Warranty BAS warrants equipment manufactured by the company to be free from defects in material
and workmanship for a period of one year from the date of shipment, except as provided
hereinafter. This warranty assumes normal usage under commonly accepted operating
parameters. BAS agrees to either repair or replace, at its sole option and free of part charges
to the buyer, any parts of instrumentation which, under proper and normal conditions of use,
prove to be defective within one year from the date of shipment. Electrochemical cells and
working electrodes are warranted for 60 days. Expendable items including but not limited to
carbon paste, reference electrodes, source lights, panel lights, fuses, etc., are excluded from
the warranty. This warranty and remedy are given expressly and in lieu of all other
warranties, expressed or implied, including but not limited to warranties of merchantability
and fitness for particular purpose, and constitute the only warranty made by BAS.
BAS neither assumes nor authorizes any person to assume for it any other liability in
connection with the sale, installation, service, or use of its instruments.
All products manufactured by BAS are tested and inspected prior to shipment. Upon prompt
notification by the Buyer, BAS will correct any defects in warranted equipment of its
manufacture either (at our option) by return of the item to our factory or shipment of a
Section 2. General Information
2
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repaired or replacement part. BAS is not obliged, however, to replace or repair any piece of
equipment which has been abused, improperly installed, altered, damaged, or repaired by
others. Defects in equipment do not include decomposition, wear, or damage by chemical
action or corrosion. Shipping charges under warranty are covered only in one direction. The
Buyer is responsible for the cost of shipping to the factory if return of the part is required.
BAS shall have no liability whatsoever for special, consequential, or punitive damages of
any kind arising from the sale, installation, use, or servicing of its instruments.
This instrument is manufactured for research purposes only. Use in medical diagnosis is not
intended, implied, or recommended by the manufacturer. Use for this purpose and
accountability for the same rest entirely with the user.
Service BAS provides a skilled service staff to solve your equipment-oriented problems. For further
details, call Customer Service at [phone] or e-mail [e-mail].
Following discussion of your specific difficulties, an appropriate course of action will be
described and the problem resolved accordingly. Do not return any products for service until
you have a return authorization number (RA#). Turn-around time on service can be
estimated at the time your RA# is issued, but actual service required cannot be determined
until we have received your unit and verified the problem. All correspondence and
shipments should be sent to:
RA#_______, Service Department
BAS
2701 Kent Avenue
West Lafayette, IN 47906
Section 2. General Information
3
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Section 3. Installation
Inspection of Shipment After carefully unpacking the instrument, check the contents of the packages and inspect for
damage. Parts of the C3 Cell Stand are listed below. (This list is subject to change.) Please
refer to the packing slip with your instrument to verify the parts. Retain the shipping box and
packing material until you have fully tested the unit to be certain that no damage was
incurred during shipment.
If you discover a shortage in parts, call BAS Customer Service and describe the shortage.
Replacement part(s) will be sent immediately, subject to stock availability.
Parts of C3 Cell Stand C3 Cell Stand with Faraday Cage
Stir bar
Glassy carbon voltammetry electrode
Platinum voltammetry electrode
Voltammetry cell vials
Polishing kit
Ag/AgCl reference electrodes (3)
Platinum wire auxiliary electrode
Port plug
Gas connection tubing
Cables
Instruction manual
Location of C3 Cell Stand 1. Provide a surge-free power source. Other laboratory equipment such as ovens, vortex mix-
ers, centrifuges, and large motors may cause spikes in the power supply.
2. Ensure that all components of the system share the same ground circuit. This can best be
accomplished by plugging all components into a multi-outlet power strip. Plugging the
components into independent outlets can produce ground loops (current that flows be-
tween ground circuits at slightly different potentials), which can produce baseline noise.
Section 3. Installation
4
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3. Locate the C3 Cell Stand on a stable bench. Vibrations can cause noise.
4. Select a room where temperature remains stable throughout the day. Avoid installing the
C3 near windows, air ducts, ovens, and refrigerators. Diffusion coefficients can change
by 3%/°C. A water-jacketed cell may be required for very precise work.
5. Place the C3 away from busy, congested areas. Remote, isolated areas are best for high-
sensitivity work.
6. Avoid very dry areas and areas that are carpeted. Static electricity can affect instrument per-
formance. Anti-static floormats and benchmats are useful if spiking caused by static
charge is a problem.
7. Avoid areas where radio-frequency interference is likely. Beeper-type paging devices can
be a problem in some installations.
Power Requirements The C3 Cell Stand contains a fused, self-sensing power supply which can be powered by
100-240 V (50-60 Hz). It uses two 2 Amp fuses. Power requirements are shown on the back
panel of the C3 (see Figure 2).
Figure 1. Fuse Placement
Section 3. Installation
5
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Back Panel Controls and
Connections
1. Cell Lead Connector.
2. Remote Connector. 25-pin D connector for remote control of C3
3. Gas Inlet Connector for Cell Purging. Bulkhead fitting providing connection to
external gas source. NOTE: 5 psi maximum.
4. Thumb screws
5. Fuse Holder.
6. Power On/Off Switch.
7. Power Cord. Be sure that the 3-prong power cord is connected to a grounded circuit.
Figure 2. Back Panel
2.0 Amp Slow Blow
100-240 VAC 50/60 Hz
FUSES:
C3 Cell Stand
Bioanalytical Systems, Inc.
Made in USA
REMOTE
CELL
Disconnect Power Before Removing Fuses.
No User Serviceable Parts Inside. DO NOT
Remove Cover. Refer Servicing To Factory
Personnel.
CAUTION:
GAS INLET
5 psi max
5
4
6
7
1
2
3
Section 3. Installation
6
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Making Connections Power Cord Connection
Push the socketed (female) end of the power cord into the port located in the back panel.
Before making this connection, make certain that the power on/off switch is in the off
position. This switch is labeled with a 0 and a 1. When 0 is pushed, the power is off and
when 1 is pushed, the power is on.
C3 to BAS 100 Series Electrochemical Analyzer
Two cables are required to connect the C3 to the BAS 100 series. Stirring and purging can
be automatically controlled by the BAS 100 series. The control cable is a 25-line ribbon
cable (ER-9515) between the REMOTE connector (25-pin D, male) on the back of the C3
and the CELL STAND connector (25-pin D, female) on the back of the BAS 100 series. See
Figure 3.
Connection of the cell lead cable is also shown in Figure 2. The cell lead cable has a
stainless steel LEMO connector at each end. One end is inserted into the CELL socket on
the back of the C3. The other end is inserted into the CELL socket in the back of the BAS
100 series. The ends are identical; therefore, it does not matter which end is connected to
the BAS 100 series or the C3.
Figure 3. Connection of C3 to BAS 100 Electrochemical Workstation
POWER ENTRY
MODULE
GAS INLET
REMOTE
CELL
Cell
Cell Stand
Section 3. Installation
7
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C3 to epsilon EC
Two cables are required to connect the C3 to epsilon EC. Stirring and purging can be
automatically controlled by the epsilon. The control cable is a 25-line ribbon cable
(ER-9515) between the REMOTE connector (25-pin D, male) on the back of the C3 and the
CELL STAND connector (25-pin D, female) on the back of the epsilon. See Figure 4.
Connection of the cell lead cable is also shown in Figure 4. The cell lead cable has a
stainless steel LEMO connector at each end. One end is inserted into the CELL socket on
the back of the C3. The other end is inserted into the CELL socket on the back of the
epsilon. The ends are identical; therefore, it does not matter which end is connected to the
epsilon or the C3.
Figure 4. Connection of C3 to epsilon
C3 to CV-1B Voltammograph
A single cable connects the C3 to the CV-1B. Stirring and purging are manually controlled.
The cell lead cable has a stainless steel LEMO connector at each end. One end is inserted
into the CELL socket on the back of the CV-1B. The other end is inserted into the CELL
socket on the back of the C3. The ends are identical; therefore, it does not matter which end
is connected to the CV-1B or the C3.
POWER ENTRY
MODULE
GAS INLET
REMOTE
Cell
Cell Stand
CELL
Section 3. Installation
8
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C3 to CV-27 Voltammograph
A single cable connects the C3 to the CV-27. Stirring and purging are manually controlled.
The connection of the cell lead cable is shown in Figure 5. The cell lead cable has a
stainless steel LEMO connector at each end. One end is inserted into the CELL socket on
the back of the C3. The other end is inserted into the CELL socket on the back of the CV-27.
The ends are identical; therefore, it does not matter which end is connected to the CV-27 or
the C3.
Figure 5. Connection of C3 to CV-27 Voltammograph
C3 to Non-BAS Instruments
The C3 should work, at least in the manual mode, with virtually any instrument. Cables
must be custom made by the user. The CELL cable is modified by cutting off one of the
LEMO connectors and attaching the appropriate connector for the particular instrument. The
color code for internal wires is red (auxiliary electrode lead), white (reference electrode
lead), black (working electrode lead), and bare (ground). Alternatively, if the instrument has
a cell lead with alligator clips, disconnect the cell leads that are in the front panel of the C3
and connect the alligator clips directly to the electrodes. If automatic control is desired, see
Section 4 for information on the pin outs of the REMOTE connector for construction of the
proper cable.
Use with Low Current Module and Impedance Module
The C3 is designed to accommodate Low Current Module and Impedance Module
accessories. Remove the cell lead box by loosening the two thumb screws (see Figure 2)
from the back panel and sliding the cell lead box out. Slide in the appropriate module and
tighten the thumb screws. It is important that the thumb screws be tight to ensure proper
grounding of the case. Connection and operation of the Low Current Module and Impedance
Module are described in their respective Instruction Manuals.
POWER ENTRY
MODULE
GAS INLET
REMOTE
Cell
CELL
Section 3. Installation
9
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Gas Inlet Connection
The C3 package contains 0.25" OD tygon tubing and a gas line fitting. One end of the gas
line fitting is barbed to fit inside the tygon tubing. Push the barbed end into the tubing. The
other end of the fitting connects to the GAS INLET port on the rear panel of the C3. To attach
the line, simply push the connector into the port. A retaining clip will snap into place. The
open end of the tygon tube is connected to a regulated gas supply. The inlet gas pressure
must not exceed 5 psi. To remove the tube from the C3, squeeze the retaining ring tab
against the connector, then pull the tube and connector away from the C3.
Figure 6. Gas Line Connection to C3
Cell Placement
The C3 is designed for easy cell replacement and to accommodate both regular cells and
water-jacketed cells. The cell is held in place by the stir motor. Follow these instructions for
initial cell placement:
1. Pivot the stir motor to the right.
2. Bring the cell up from underneath, around the electrodes, and seat on the cell top.
3. Pivot the stir motor back under the cell.
4. If required, the cell top height can be adjusted by loosening the cell height
adjustment knob, sliding the cell top assembly to the desired position, and
tightening the knob.
5. Four 0.25" holes are in the cell top. These holes are for the working electrode,
reference electrode, auxiliary electrode, and standard addition port. Place the
electrodes and port plug in the holes which are the most convenient.
Purging/Blanketing Lines
The C3 has the ability to purge or blanket the sample solution with an inert gas. The purge
removes oxygen by bubbling an inert gas, typically nitrogen or argon, through the solution.
The blanketing function is to maintain an inert atmosphere above the sample to keep oxygen
from re-entering the sample solution.
The purge/blanket lines are the two plastic tubes coming out of the cell top support block.
These two lines should be pressed into the Teflon cell top. The blanket line should extend to
just beyond the bottom of the cell top. The depth of the purge tube should be near the
bottom of the cell vial.
GAS INLET
5 psi max
Section 3. Installation
10
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Electrode Leads
The electrode lead wires extend through the front panel. The connectors are the
spring-loaded, press-on type. Simply push the connector over the corresponding pin in the
electrode to make the connection (Figure 7). Each wire is color coded to the electrode it
attaches. The code is:
Black .........Working Electrode
White ........ Reference Electrode
Red..........Auxiliary Electrode
Alligator clips with the appropriate pin and colored boot are included for connection to
electrodes that cannot be connected to the sockets on the cell lead.
Figure 7. Electrode Lead Connections
CONNECT TO BLACK
(WORK) LEAD
CONNECT TO
RED (AUX) LEAD
CONNECT TO WHITE
(REF) LEAD
Section 3. Installation
11
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Section 4. Operation
Polarity of Remote Stir
Control
The factory-set polarity for control of the remote stir line is active low (e.g., low signal turns
stir ON, high signal turns stir OFF). If opposite stir control polarity is needed (e.g., active
high), then remove the jumper on the C3 printed circuit board (Figure 8) and place it on
only one pin. The jumper is accessible after removing the side panel near the switches on
the C3.
Figure 8. Location of Remote Stir Control Polarity Jumper
Front Panel Controls and
Connections
1. Power LED. LED is lit when C3 is powered (on/off switch is located on back panel).
2. Stir-LED. LED is lit when stir is turned on, either manually or remotely.
3. Stir Control. Switch to manually control stirrer or to select remote control.
4. Stir Rate Switch. Switch for selection of one of 16 available stir rates.
5. Gas Purge LED. LED is lit when gas purge is turned on for sample vial, either
manually or remotely. When LED is off the gas flows through the blanket line.
6. Gas Purge Control. Switch to manually control gas flow to purge or blanket lines or
to select for remote control.
7. Gas Purge Flow Control. Needle valve control of gas flow to purge and blanket lines.
8. Latch/Ground Socket for Faraday Cage.
9. Reference Electrode Lead. White lead connected to reference electrode.
10. Gas Purge and Blanket Lines.
11. Working Electrode Lead. Black lead connected to working electrode.
12. Auxiliary Electrode Lead. Red lead connected to platinum wire auxiliary electrode.
13. Standard Addition Port.
14. Cell Top Height Adjustment Knob.
15. Stir Motor.
JMP1
Section 4. Operation
12
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Figure 9. Front Panel
Cell Stand
POWER
PURGE
ON
Remote
Blanket
Remote
OFF
ON
STIR
50
100
150
200
250
300
350
400 450
500
550
600
650
700
750
800
RPM
C3
11
12
13
9
14
15
10
8
1
5
7
6
4
2
3
Section 4. Operation
13
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Remote Control The remote connector of the C3 allows the stirring and gas purge functions to be controlled
by an external unit. The cell stand was specifically designed to be controlled by the
appropriate commands from the BAS 100 Series Electrochemical Analyzer and CV-50W.
These functions can be activated by either a TTL signal or from any controller providing a
contact closure to the ground. All lines are active low (negative edge triggered) and the pin
designations of the remote connector are as follows:
Pin Description
4 digital ground
7 digital ground
8STIR
21 PURGE
Operation of these lines is also dependent on the setting of the stir and purge switches on
the front panel.
Gas Purge The flow rate of the gas and whether it is directed to the purge or blanket lines is controlled
by a front panel knob and switch. When toggled to the ON position, the gas flow is directed
to the purge line and cannot be changed by remote control. When switched to the
REMOTE/BLANKET position, the gas is directed to the blanket line. It must be in this
position to externally control PURGE via the REMOTE connector on the back panel. The LED
is lit when the purge function is on, either manually or remotely.
Stir The C3 has a precisely controlled magnetic stirrer. The stirrer can be controlled either by the
front panel switches or remotely. When the stir switch is toggled to the ON position, the
stirrer is on at the rate selected by the rate switch (50-800 RPM) and cannot be remotely
controlled. The REMOTE position is required for remote control of the stirrer. The LED is lit
when the stir function is turned on, either manually or remotely.
Faraday Cage The C3 is supplied with an easily removed Faraday Cage. The Faraday Cage pivots on
lift-off hinges. The latch grounds the Faraday Cage; thus, the door must be completely
closed for it to be effective. For many experiments, the signal generated at the electrode is
large enough that the Faraday Cage is not necessary. When small signals must be
measured, the Faraday Cage will shield the cell from many electrical interferences.
Unconnected wires and water lines for temperature control passing through the wall of the
Faraday Cage may act as antennas and be sources of line frequency interference for small
signals. For best results, remove any unconnected wires and properly ground water lines.
Removal of Cell Lead Box Remove the cell lead box by loosening the two thumb screws from the back panel (see
Figure 2) and sliding the cell lead box out. When replacing the thumb screws, tighten well
to ensure proper grounding of the case.
Section 4. Operation
14
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Section 5. Maintenance
General Maintenance The C3 is a very rugged instrument and, with proper care, should give years of service.
Following is a brief list of cautions and general maintenance considerations that will extend
the lifetime of the instrument.
• Follow customary, good laboratory practices.
• Clean all spills, especially salt solutions, on or near the cabinet immediately.
• Avoid placing the unit in a corrosive atmosphere.
• Avoid dropping, shaking, or other forms of mechanical abuse to prevent
loosening of components or subassemblies.
• Clean gas lines (rinse and wipe dry) after each use.
• Do not bend the auxiliary electrode (platinum wire) when removing or placing the
cell vial. Repeated bending will cause the wire to break.
Troubleshooting Symptom Cause Solution
saturated signal reference tip not in lower reference
contact with solution
noisy signal clogged reference frit replace reference
air bubble in “flick” reference until bubbles
reference tip are at top
vibrations place on pad to dampen vibration
electrical line use Faraday Cage
frequency interference
spikes in power line power from a different circuit
variation in temperature variation use water-jacketed cell
peak height
Service Procedure There are no user-serviceable electronic parts in this unit and all service requests should be
referred to BAS service personnel. In certain cases, BAS will provide electronic schematics
and service procedures to qualified electronic maintenance facilities, but only upon written
request and then only with the approval of the Service Coordinator.
If a problem arises and appears equipment oriented, call BAS at [phone] and ask for
Customer Service, or e-mail [e-mail].
Section 5. Maintenance
15
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Artisan Technology Group is your source for quality
new and certied-used/pre-owned equipment
• FAST SHIPPING AND
DELIVERY
• TENS OF THOUSANDS OF
IN-STOCK ITEMS
• EQUIPMENT DEMOS
• HUNDREDS OF
MANUFACTURERS
SUPPORTED
• LEASING/MONTHLY
RENTALS
• ITAR CERTIFIED
SECURE ASSET SOLUTIONS
SERVICE CENTER REPAIRS
Experienced engineers and technicians on staff
at our full-service, in-house repair center
WE BUY USED EQUIPMENT
Sell your excess, underutilized, and idle used equipment
We also offer credit for buy-backs and trade-ins
www.artisantg.com/WeBuyEquipment
REMOTE INSPECTION
Remotely inspect equipment before purchasing with
our interactive website at www.instraview.com
LOOKING FOR MORE INFORMATION?
Visit us on the web at www.artisantg.com for more
information on price quotations, drivers, technical
specications, manuals, and documentation
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