Hygiena DUP-1000 User manual

Model DUP-1000
User Guide

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transmitted in any form, or by any information storage and retrieval system, without the prior agreement and written
permission of Hygiena.
Information in this document is subject to change without notice. Hygiena assumes no responsibility for any errors that
may appear in this document. This document is believed to be complete and accurate at the time of publication. In no
event shall Hygiena be liable for incidental, special, multiple or consequential damages in connection with or arising from
the use of this document.
Part Number 2C-075.1-0314
TRADEMARKS
The Hygiena logo and BAX®are registered trademarks of Hygiena or its affiliates.
All other trademarks are the property of their holders.
FIELD OF USE
The Automated Thermal Block is manufactured by Torrey Pines Scientific for use with the BAX System. Please see BAX
System documentation for details on Field of Use. Please read the Limitation of Warranty and Liability before using the
product.
WARRANTY
This product is warranted by Torrey Pines Scientific to be free from defects in material and workmanship for a period of
one year from the date of purchase. If repair or adjustment is necessary and has not been the result of abuse or misuse
within the one-year period, correction of the defect will be made without charge.
For warranty and repair issues, contact Torrey Pines Scientific at [phone].
TECHNICAL SUPPORT
If you have any questions or comments on the Automated Thermal Block, please contact your distributor or Hygiena at
800-863-6842 or email [e-mail] for technical assistance.

TABLE OF CONTENTS
Instrument Overview........................................................................................................... 1
Supplied Components ........................................................................................................ 1
Specifications and Requirements....................................................................................... 2
Safety Symbols and Precautions........................................................................................ 2
Connecting the Thermal Block ........................................................................................... 3
Front Panel Display............................................................................................................. 4
Program Menu .................................................................................................................... 5
Turning the Thermal Block On and Off............................................................................... 5
Running the Gram Positive Program.................................................................................. 6
Running the Gram Negative Program................................................................................ 8
Running the 24E Program ................................................................................................ 10
Running the RT Listeria Program..................................................................................... 14
Cleaning and Decontaminating the Thermal Block.......................................................... 16
Calibrating the Thermal Block .......................................................................................... 16
Tips and Troubleshooting ................................................................................................. 22
Aborting a Program.................................................................................................. 22
Error Messages........................................................................................................ 23

User Guide 1
www.hygiena.com INS2051 Rev A
Instrument Overview
The Automated Thermal Block is a Peltier-driven heating and cooling device pre-programmed to
perform the lysis steps of the BAX System protocols. It has been designed as a solid-state device
with minimal moving parts to help ensure quality performance over time. The temperature of the
plate is sensed by a platinum resistance temperature device mounted under the plate. The
computer in the unit compares the plate temperature with the target temperature and instructs the
module to heat or chill the plate as required.
The block automatically provides the sequential heating and cooling conditions for lysis protocols,
eliminating the need to transfer samples between separate heating and cooling blocks. Once the
lysis program is complete, the block holds the samples at 4 °C until they are removed.
This model has been optimized for use with the cluster tubes and metal tube holder supplied with
the BAX System. Other tubes and holders are not recommended.
Supplied Components
The purchase of the thermal block includes the following components:
1. Thermal block unit
2. Metal tube holders (2)
3. Power supply and cord
4. User documentation

2 Automated Thermal Block
Specifications and Requirements
Item Specification
Dimensions (with lid) 6.5″W x 8.75″D x 8″H (16.5 x 22.25 x 20.3 cm)
Weight Approximately 8 lbs. (3.6 kg)
Power usage 12 volts, 8.4 amps
Power requirements 90 to 265 volts AC, 50/60Hz
Thermal range -10 °C to 110 °C (14 to 230 °F)
Thermal uniformity ±1 °C as measured at the block surface
Thermal accuracy ±1 °C as measured at the block surface
Sample throughput 1-96 samples per cycle
Room environment Temperature between 65 and 89 °F (18 and 32 °C)
Safety Symbols and Precautions
Safety Symbols
Symbol Description
Indicates that you should consult the manual for further information and proceed with
appropriate caution.
Indicates the presence of a hot surface or other high-temperature hazard and
proceed with appropriate caution.
Indicates the presence of an electrical shock hazard and proceed with appropriate
caution.
General Safety Information
Extreme Temperatures
The thermal block is capable of reaching 110 ºC (230 ºF), which can burn
the skin if touched.
To prevent injury, remember the following:
•Use extreme caution around the block at all times.
•Never leave the block accessible to others when it is hot.
•Do not touch the plate surface unless you are sure it has cooled.
•Always keep the lid in place when the block is not in use.
Power Requirements
Always use the supplied AC power cord when connecting the heating
block to a power source. Use the normal care and precaution that one would
use with any electrical appliance.

User Guide 3
www.hygiena.com INS2051 Rev A
Connecting the Thermal Block
The Automated Thermal Block is pre-programmed to perform the sequential heating and cooling
steps needed for BAX System assays. No calibration or software installation is required when the
thermal block is used as specified.
Follow these steps to connect the thermal block:
1. Place the thermal block unit on a level, dry laboratory workbench. Allow at least 4 inches
(10 cm) on all sides of the unit for ventilation.
2. Insert one end of the provided power cord into the power supply and plug the other end
of the power cord into a 3-wire outlet.
Note: Ensure the outlet is properly grounded and runs the appropriate voltage (see specifications
on the previous page).
3. Insert the power supply jack into the power input port on the back panel of the thermal
block.
4. Seat the metal tube holder over the metal plate surface on the top of the thermal block
unit.

4 Automated Thermal Block
Front Panel Display
The front panel of the Automated Thermal Block contains the following items:
1. LCD display – shows the program menu, program selection, settings and instructions.
2. SCROLL button – changes the selected menu item in the LCD display. An audible beep
sounds when the button is pressed.
3. SELECT/CONTINUE button – sets the menu item in the LCD display as the selected
program and is also used to confirm required actions. An audible beep sounds when the
button is pressed.
4. Blue ACTION NEEDED LED – indicates that the user must perform an action before the
program can continue. This notification is accompanied by an audible alarm.
5. Green POWER LED – indicates that the thermal block is turned on.

User Guide 5
www.hygiena.com INS2051 Rev A
Program Menu
The Automated Thermal Block is pre-programmed with four lysis protocols:
Gram positive – use with Gram-positive bacteria, including Staphylococcus and Listeria*
*Note: For Listeria 24E assays, use the “24E” program. For real-time Listeria assays, use the “RT Listeria” program.
Gram negative – use with Gram-negative bacteria, including Salmonella, E. coli,
Campylobacter, Vibrio and Cronobacter
24E – use with BAX System 24E assays for Listeria and L. monocytogenes
RT Listeria – use with BAX System real-time assays for Listeria and L. monocytogenes
Turning the Thermal Block On and Off
ON Turn on the thermal block by toggling
the on/off switch on the back panel of
the unit.
At start-up, the LCD automatically
displays the following series of
messages:
The first screen shows the version of
software currently installed on the
thermal block.
The second screen shows the seq file
version number and the checksum
number for validation purposes.
Note: A change in either of these values
indicates a modification to the thermal
block software. If this occurs, any
validation previously performed on the
instrument may no longer apply.
The start-up sequence is complete
when the LCD display shows the
program menu.
OFF After finishing a run, you can either
select another program or turn off the
power to the unit by toggling the on/off
switch on the back panel of the unit.
Hygiena Thermal
Block v3.2
seq file ver: 02
checksum: EE
Gram Positive
Gram Negative

6 Automated Thermal Block
Running the Gram-Positive Program
Samples are heated to 55 °C for 60 minutes, then 95 °C for 10 minutes, then held at 4 °C for at
least 5 minutes or until removed.
1 Turn on the thermal block, then press the
SCROLL button until the arrow

is next to the
Gram Positive option.
Press the SELECT/CONTINUE button.
2 The unit beeps once and the LCD display changes
as the thermal block begins equilibrating to 55 °C.
Note: Do not load samples into the thermal block
until the load prompt appears on the LCD display.
3 When the thermal block has reached 55 °C, the
unit sounds two audible beeps and the blue
ACTION NEEDED LED activates. The LCD
display changes to “Load Samples”.
4 After the “Load Samples” prompt appears, place a
rack of prepared samples into the metal tube
holder.
Note: See the BAX System User Guide for information
on preparing samples.
Press the SELECT/CONTINUE button.
5 The LCD display changes to “GmPos Lysis 55C”.
A timer in the bottom right corner counts down
from 60:00 and shows the number of minutes
remaining in this step.
6 After lysis at 55 °C is complete, the LCD display
changes to “Heating to 95C”. A timer in the bottom
right corner counts up from 0:00 during the
temperature change. No user action is required.
7 After the thermal block reaches 95 °C, the LCD
display changes to “Denaturing @ 95C”. A timer in
the bottom right corner counts down from 10:00
and shows the number of minutes remaining in
this step. No user action is required.
Gram Positive
Gram Negative
Equilibr.@ 55C
GmPos
Load Samples
GmPos
GmPos Lysis 55C
GmPos 57:08
Heating to 95C
GmPos 4:39
Denaturing @95C
GmPos 7:22

User Guide 7
www.hygiena.com INS2051 Rev A
8 After denaturation at 95 °C is complete, the LCD
display changes to “Cooling to 4C”, and the
thermal block sounds two audible beeps to signal
the change. A timer in the bottom right corner
counts up from 0:00 during the temperature
change. No user action is required.
9 After the samples have cooled for 5 minutes, the
LCD display changes to “Sample PCR Ready”, the
blue ACTION NEEDED LED activates and the
thermal block sounds four audible beeps to signal
that samples can be removed.
Press the SELECT/CONTINUE button.
Note: A timer in the bottom right corner
counts up from 0:00 to show any
additional time that samples are held at
4 °C.
10 The LCD display changes to “Completed” and the
thermal block sounds an audible beep to signal
that the program has been completed. The timer
continues to show any additional time that
samples are held at 4 °C.
11 Follow the instructions in the BAX System User
Guide to hydrate the PCR tablets with these
lysates.
Note: Lysates must be maintained at 4 °C
during tablet hydration.
12 After the PCR tablets have been hydrated, remove
the rack of cluster tubes from the metal tube
holder.
Press the SELECT/CONTINUE button.
13 The LCD display changes to “Gram Positive
Finished” and the thermal block sounds an audible
beep to signal that the program has finished.
14 Press the SELECT/CONTINUE button to exit from
the program. The LCD changes to display the
program menu.
Cooling to 4C
GmPos 1:45
Sample PCR Ready
GmPos 1:06
Completed
GmPos 6:25
Gram Positive
**Finished**
Gram Positive
Gram Negative