Accuris SmartDrop L NS1000 User manual

Operation Manual
V1.0
NS1000

Thank you for purchasing the SmartDrop™ L Nano Spectrophotometer. This user
manual details the instrument’s features, specifications, as well as complete
operating instructions; please read it carefully before operation. Keep this user
manual for later use.
Important:
Please keep the box and packaging material for this instrument. If service is
required, the box will be needed to ship the instrument to our Service
Department.
Initial Inspection
Please inspect the instrument as well as all included accessories when you first
open the packaging. If you find anything damaged or missing, please contact
Benchmark Scientific or your local distributor immediately.
BENCHMARK SCIENTIFIC / ACCURIS
INSTRUMENTS
PO Box 709
Edison, NJ 08818
USA
Phone: [phone]
Website: www.benchmarkscientific.com
/ www.accuris-usa.com

Safety Warnings and Guidelines
1. Important information for safe use
Users should understand how to use this instrument before operating.
Please read this manual carefully prior to operation.
Any improper operation may cause injury. Please read this
manual carefully and operate safely according to the guidelines.
2. Operation and Maintenance
The operation and maintenance of the instrument should comply with the basic
guidelines and warnings below. Incorrect operation or maintenance will have
detrimental effects on the life, performance, and safety features of the instrument.
The instrument is a normal indoor instrument which conforms to
classⅠof the GB 4793.1 standard.
This instrument is designed for use in a laboratory
environment. The device must be operated by skilled
laboratory personnel with appropriate training.
To prevent injury or voiding the warranty, the operator should not
attempt to repair the instrument without explicit guidance from
Accuris Instruments. If service is required, please contact Accuris
Instruments or your local distributor for repair.
Before powering on, confirm that the voltage used meets the
electrical requirements of the instrument as stated on the rating
plate. If the electric cord is damaged, replace it with the same type
of cord. Hold the socket firmly before pulling the plug from an outlet.
Do not pull the electric cord.
The instrument should be installed in an environment of standard
room temperature, low dust, low humidity, and away from direct
sunlight, electromagnetic interference, and heat sources. Do not
block the vents on the instrument.

Always power off the instrument when you are finished using it.
Unplug the power cord and cover the instrument with a cloth or
plastic sheet to prevent excessive dust from entering the housing.
Pull the connector plug from the electrical outlet immediately and
contact the vendor in the event of:
•Liquid entering the housing.
•Abnormal operation: such as any abnormal sound or
smell.
•The instrument is dropped or there is any damage to the
housing.
•Any malfunction.
3. Maintenance
The pedestal should be cleaned regularly using a soft cloth dampened with
deionized water. The instrument housing should be cleaned regularly using a soft
cloth dampened with a small amount of alcohol.

Table of Contents
Chapter 1 Introduction ....................................................................................... 1
1.Key Features ............................................................................................... 1
Chapter 2 Specifications .................................................................................... 2
1.Required Installation Environment ............................................................... 2
2.Specifications............................................................................................... 2
Chapter 3 Instrument Overview ......................................................................... 3
1.Structure ...................................................................................................... 3
2.Sample Size Requirements ......................................................................... 4
3.Dispensing Samples onto The Lower Pedestal ........................................... 4
4.OD600 Measurement .................................................................................. 5
Chapter 4 Programming & Operation................................................................ 6
1.Start-up Interface ......................................................................................... 6
2.Main Menu Interface .................................................................................... 6
3.Nucleic Acids Interface ................................................................................ 7
4.Protein A280 Interface ................................................................................11
7.OD600 Interface ........................................................................................ 13
8.System Settings Interface.......................................................................... 15
Chapter 5 Troubleshooting .............................................................................. 17

1
Chapter 1 Introduction
The SmartDrop™ L nano spectrophotometer measures 1.0µL – 2.0µL samples with
high accuracy and reproducibility and employs surface tension to position the sample
for measurement. The system includes a cuvette slot for OD600 readings & a built-in
printer for printing results. The SmartDrop L can measure highly concentrated samples
without dilution (100X concentration of samples measured by a standard cuvette
spectrophotometer).
1. Key Features
➢User-Friendly Input & Operation – Touch screen for programming and operation (a
mouse can also be connected).
➢Multifunctional software for Nucleic Acids, Protein A280, & OD600 measurements.
➢dsDNA detection range from 10ng/µL – 2500ng/µL.
➢Fast & accurate measurements (< 6 seconds).
➢2 cuvettes included (optical glass) for OD600 measurements.

2
Chapter 2 Specifications
1. Required Installation Environment
Environmental Temperature: 5°C～35°C
Relative Humidity: ≤ 70%
Input Voltage: DC 24V, 2A ( Adapter CSA, UL, CE marked)
2. Specifications
Model
SmartDrop™ L (NS1000)
Minimum Sample Size
1.0μL – 2.0μL
Path Length
0.5mm
Light Source / Life
UV LED / 8000h
Detector Type
UV Silicon Photocell
Wavelength Range
260, 280, 600nm
Wavelength Accuracy
± 1 nm
Absorbance Precision
0.005Abs
Conc. Accuracy
±5ng [10,500)
±2% [500,2000)
±3% [2000,2500]
Absorbance Range
0.2-50 (10mm equivalent)
Detection
Concentration Range
10ng/μL dsDNA ～ 2,500ng/μL dsDNA
Detection Time
< 6 seconds
OD600
Abs range
0~4.000 Abs
Abs stability
[0,3）≤0.5% [3,4）≤2%
Abs
repeatability
[0,3）≤0.5%, [3,4）≤2%
Abs Precision
[0,2）≤0.005A, [2,3）≤1%， [3,4）≤2%
Voltage input
DC 24V, 2A
Power
25W
Dimensions（W×D×H）
20.8cm × 28.0cm ×18.6cm / 8.2in x 11.0in x 7.3in
Weight
3.6 kg / 7.9 lbs

3
Chapter 3 Instrument Overview
1. Structure
Front
Fig. 1 Front
Back
Fig. 2 Back
USB ports
Switch
Power Socket
Lower pedestal
Upper pedestal
Display
Cuvette slot
Built-in printer

4
2. Sample Size Requirements
Surface tension is a critical factor in the formation of the sample column for
measurement. The hydrophobic interactions between water molecules in a sample
solution is key in creating & maintaining surface tension. The presence of solutes
(proteins, DNA, RNA, salt ions, detergent molecules) significantly reduces surface
tension and hinders the formation of the sample column. For most samples, a 1μL
sample size is enough; however, to ensure accurate and precise measurements, a 2μL
sample size is recommended to allow the formation of the sample column for
measurement.
To ensure precise and accurate measurements, it is essential that a complete liquid
column forms between the upper pedestal and lower pedestal. It is recommended that
a precision pipettor (0-2μL) be used to dispense samples.
3. Dispensing Samples onto The Lower Pedestal
Lift the upper pedestal and pipette the sample (1.0µL –2.0μL) onto the lower
pedestal (Fig. 3).
Fig. 3 Dispense Sample Fig. 4 Sample Drop
Lower the upper pedestal onto the sample to form the sample column (Fig. 5).
Fig. 5 Sample Column
Note: Please exercise caution when lowering the upper pedestal onto the sample.