Metrohm 753 User manual

CH-9101 Herisau/Switzerland
Tel. ++41 71 353 85 85
Fax ++41 71 353 89 01
Internet www.metrohm.ch
E-Mail [email protected]
753 Suppressor Module
Suppressor Module753 MetrohmMetrohm
ON ON
OFFOFF
REMOTE PUMP
POWER
ON
OFF
REMOTE
STEP
SUPPRESSOR
8.753.1003 Instructions for Use
29.11.96 / dö

Table of contents
753 Suppressor Module / 8.753.1003 Instructions for Use I
Table of contents
1 Introduction............................................................................................1
1.1 Instrument description.............................................................................1
1.2 Parts and controls.....................................................................................2
1.3 Information about the Instructions for Use..........................................4
1.3.1 Organization..................................................................................4
1.3.2 Notation and pictograms...............................................................5
1.4 Safety notes ...............................................................................................6
1.4.1 Electrical safety..............................................................................6
1.4.2 General precautionary rules..........................................................6
2 Installation..............................................................................................7
2.1 Setting up the instrument........................................................................7
2.1.1 Packaging......................................................................................7
2.1.2 Check ............................................................................................7
2.1.3 Location.........................................................................................7
2.1.4 Arrangement of the instruments....................................................7
2.2 Mains connection......................................................................................8
2.2.1 Setting the mains voltage..............................................................8
2.2.2 Fuses.............................................................................................9
2.2.3 Mains cable and mains connection..............................................9
2.2.4 Switching the instrument on/off.....................................................9
2.3 Connection to the 690 Ion Chromatograph........................................10
2.3.1 Electrical connection...................................................................10
2.3.2 Connection of the suppressor block...........................................10
2.4 Connection to the 732/733 IC system..................................................15
2.4.1 Electrical connection...................................................................15
2.4.2 Connection of the suppressor block...........................................15
3 Operation................................................................................................19
3.1 Handling the suppressor .......................................................................19
3.2 Manual operation.....................................................................................20
3.3 Operation via 690 Ion Chromatograph................................................21
3.4 Operation via 732 IC Detector...............................................................22
4 Malfunctions – Maintenance...........................................25
4.1 Malfunctions and their rectification.....................................................25
4.2 Maintenance and servicing....................................................................27
4.2.1 Care.............................................................................................27
4.2.2 Maintenance by Metrohm service...............................................27
4.2.3 Shutdown.....................................................................................27
4.2.4 Exchanging the pump tubing......................................................28
4.2.5 Regeneration of the suppressor..................................................29
4.2.6 Cleaning the suppressor.............................................................30
4.2.7 Exchanging the suppressor........................................................32
4.2.8 Braking range adjustment...........................................................33

Table of contents
753 Suppressor Module / 8.753.1003 Instructions for Use
II
5 Appendix .................................................................................................35
5.1 Technical data .........................................................................................35
5.2 Standard equipment...............................................................................37
5.3 Optional accessories..............................................................................38
5.4 Warranty and conformity.......................................................................39
5.4.1 Warranty...................................................................................... 39
5.4.2 EU Declaration of conformity......................................................40
5.4.3 Certificate of conformity and system validation..........................41
5.5 Index..........................................................................................................43
List of figures
Fig. 1:Front of the 753 Suppressor Module ................................................................2
Fig. 2:Rear of the 753 Suppressor Module................................................................. 3
Fig. 3:Suppressor block (1.753.0100).........................................................................4
Fig. 4:Setting the mains voltage..................................................................................9
Fig. 5:Connection 753 Suppressor Module – 690 Ion Chromatograph....................10
Fig. 6:Connections at suppressor.............................................................................11
Fig. 7:Installing pump tubings................................................................................... 13
Fig. 8:Connection 753 Suppressor Module – 732/733 IC system............................ 15
Fig. 9:Assembling the suppressor ............................................................................ 31
Fig. 10:Adjusting the braking range............................................................................34

1.1 Instrument description
753 Suppressor Module / 8.753.1003 Instructions for Use 1
1 Introduction
1.1 Instrument description
The 753 Suppressor Module consists of a suppressor block with its
corresponding control unit; this contains an additional 2-channel peri-
staltic pump.
Ion chromatographic analyses with chemical suppression can be car-
ried out with the suppressor built into the suppressor block. The sup-
pressor consists of a total of 3 suppressor units which are in turn used
for suppression, regenerated with sulfuric acid and rinsed with water. In
order to record each new chromatogram under comparable conditions
a freshly regenerated suppressor unit is normally used. Switching is ei-
ther carried out automatically together with valve switching or manually.
At the control unit the suppressor can either be switched to the next
position manually or by remote control via the remote interface. The
built-in peristaltic pump, which delivers the solutions required for oper-
ating the suppressor, can also be switched on by remote control or
manually.
The 753 Suppressor Module is particularly suitable for retrofitting the
690 Ion Chromatograph for chemical suppression in a simple manner.
This application is described in detail in these instructions for use.
The 753 Suppressor Module can also be used together with the 732 IC
Detector and the 733 IC Separation Center (e.g. for the realization of a
complete two-channel IC system with only one 733 Separation Center).
Of course the 753 Suppressor Module can also be combined with all
other HPLC components currently on the market.

1 Introduction
753 Suppressor Module / 8.753.1003 Instructions for Use
2
1.2 Parts and controls
Suppressor Module753 MetrohmMetrohm
ON ON
OFFOFF
REMOTE PUMP
POWER
11 44 55
9911111212 1010
ON
OFF
REMOTE
STEP
SUPPRESSOR
22 33 66 77 88
Fig. 1: Front of the 753 Suppressor Module
11 Power on lamp
Lights up when instrument is
switched on
77 Suppressor position display
Is lit up when the suppressor is "in
position"
22 Pump Remote ON/OFF
On/Off switch for remote control of
the pump
88 Suppressor step
Switches the suppressor to the next
position (manual operation mode)
33 Pump ON/OFF
On/Off switch for the pump (manual
operation mode)
99 Holding clamp
For locking the tubing cartridge into
place
44 Tubing cartridge
For 6.1826.050 Pump tubing 1010 Snap-action lever For releasing the tubing cartridge
55 Contact pressure lever
For adjusting the contact pressure 1111 Pump drive Roller head with contact rollers
66 Suppressor Remote ON/OFF
On/Off switch for remote control of
the suppressor
1212 Mounting pin
For attaching the tubing cartridge

1.2 Parts and controls
753 Suppressor Module / 8.753.1003 Instructions for Use 3
WARNING - Fire Hazard -
with the same type and rating of fuse
For continued protection replace only
Type 1.753.0010 Nr.
Made by Metrohm Herisau Switzerland
14141313
1919 1515
Remote
110V-120V
220V-240V 0,5A(T) 0,25A(T)
50 - 60 Hz 50 VA
PumpSuppr.
SuppressorBreakpoint
∆∆tACB
2020 161617171818
S
Fig. 2: Rear of the 753 Suppressor Module
1313 Mains switch
For switching the instrument on/off:
I = ON 0 = OFF
1717 Connection for suppressor block
1414 Serial number 1818 Adjustment screw for braking
range
1515 Remote interface for pump
Remote I/O lines for connection of
external devices (e.g. 732 IC Detector)
1919 Mains connection plug
Mains connection, see section 2.2
1616 Remote interface for suppressor
Remote I/O lines for connection of
external devices (e.g. 732 IC Detector)
2020 Fuse holder
Changing the fuses, see section 2.2

1 Introduction
753 Suppressor Module / 8.753.1003 Instructions for Use
4
1
3
2
2121 2222 2323
Fig. 3: Suppressor block (1.753.0100)
1.3 Information about the Instructions for Use
Please read through these Instructions for Use carefully before you put
the 753 Suppressor Module into operation. The Instructions for Use
contain information and warnings to which the user must pay attention
in order to assure safe operation of the instruments.
1.3.1 Organization
These 8.753.1003 Instructions for Use for the 753 Suppressor Mod-
ule provide a comprehensive overview of the startup procedure, opera-
tion, fault rectification and technical specifications of these instruments.
The Instructions for Use are organized as follows:
Section 1 Introduction
General description of instruments, parts and controls
and safety notes
Section 2 Installation
Setting up, mains connection, attachment of accesso-
ries, connection to IC system
Section 3 Operation
Manual operation and operation via 690 Ion Chroma-
tograph or 732 IC Detector
Section 4 Faults – Maintenance
Fault rectification, maintenance
Section 5 Appendix
Technical data, standard equipment, options, warranty,
declarations of conformity, index
To find the required information on the instruments please use either
the Table of contents or the Index at the back.
2121 Suppressor block
2222 Suppressor connection
with permanently fixed inlet and
outlet capillaries
2323 Connection cable
Connection cable to control unit

1.3 Information about the Instructions for Use
753 Suppressor Module / 8.753.1003 Instructions for Use 5
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these In-
structions for Use:
<PUMP> Switch or key
1515 Part or control of 753
5252 Part or control of 690
8989 Part or control of 732/733
"Range"Parameter or entry value
at 732 IC Detector
>PARAM/detector
range: 1.00 mS/cm
Display
Text in display 11 of the 732 IC
Detector
Hazard
This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed cor-
rectly.
Caution
This symbol marks important infor-
mation. First read the associated
directions before you continue.
Comment
This symbol marks additional in-
formation and tips.

1 Introduction
753 Suppressor Module / 8.753.1003 Instructions for Use
6
1.4 Safety notes
1.4.1 Electrical safety
While electrical safety in the handling of the 753 Suppressor Module is
assured in the context of the specifications IEC 1010-1 (protection class
I, degree of protection IP20), the following points should be noted:
• Mains connection
Setting the mains voltage, checking the mains fuse and the mains
connection must be effected in accordance with the instructions in
section 2.2.
• Opening the 753 Suppressor Module
When the 753 Suppressor Module is connected to the power supply
the instrument must neither be opened nor should parts be removed
from it, otherwise there is a danger of coming into contact with com-
ponents which are live. Always disconnect the instrument from all
voltage sources before you open it and ensure that the mains cable
is disconnected from mains connection 1919 !
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 753 Suppressor Module, you should earth yourself and any
tools you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.
1.4.2 General precautionary rules
• Handling of solvents
Check all pump tubings and all input and output lines periodically for
possible leaks. Follow the relevant instructions regarding the handling
of flammable and/or toxic solvents and their disposal.
• Regular exchange of pump tubings
Pump tubings are consumable material and must be replaced from
time to time (see Section 4.2.4). Suitable measures must be taken so
that any leak which might occur in the pump tubing or connections
during unattended operation will cause no damage (placing the
instrument at the bottom, collection device for any liquid which may
leak out).

2.1 Setting up the instrument
753 Suppressor Module / 8.753.1003 Instructions for Use 7
2 Installation
2.1 Setting up the instrument
2.1.1 Packaging
The 753 Suppressor Module is supplied together with the separately
packed accessories in special packagings containing shock-absorbing
foam linings designed to provide excellent protection. The actual in-
strument is packed in an evacuated polyethylene bag to prevent the in-
gress of dust. Please store all these special packagings as only they
can assure damage-free transport of the instrument.
2.1.2 Check
After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of
accessories in section 5.2). In the case of transport damage, see
instructions in section 5.4.1 "Warranty".
2.1.3 Location
Position the instruments in the laboratory at a location convenient for
operation, free from vibrations and protected against a corrosive
atmosphere and contamination by chemicals.
2.1.4 Arrangement of the instruments
The best position for the 753 Suppressor Module is beside the 690 Ion
Chromatograph and below the 709 IC Pump.
The 753 Suppressor Module should always be placed at the bottom
of the instrument stack so that any leaks which may occur in the
pump tubing or connections cannot cause damage to the other
instruments by leakage of corrosive liquids (e.g. H2SO4).

2 Installation
753 Suppressor Module / 8.753.1003 Instructions for Use
8
2.2 Mains connection
Follow the instructions below for connecting to the power supply. If
the instrument is operated with the mains voltage set wrongly and/or
wrong mains fuse there is a danger of fire!
2.2.1 Setting the mains voltage
Before switching on the 753 Suppressor Module for the first time, check
that the mains voltage set on the instrument (see Fig. 2) matches the
local mains voltage. If this is not the case, you must reset the mains
voltage on the instrument as follows:
1Disconnect mains cable
Disconnect mains cable from mains connection plug 1919 of the
753 Suppressor Module.
2Remove fuse holder
Using a screwdriver, loosen fuse holder 2020 below the mains
connection 1919 and take out completely.
3Check fuse
Carefully take the fuse installed for the desired mains voltage out
of fuse holder 2020 and check its specifications (the position of the
fuse in the fuse holder is marked by the white arrow imprinted
next to the mains voltage range):
100……120 V 0.5 A (slow-blow) Metrohm No. U.600.0013
220……240 V 0.25 A (slow-blow) Metrohm No. U.600.0010
4Insert fuse
Change fuse if necessary and reinsert in fuse holder 2020.
5Install fuse holder
Depending on the desired mains voltage, insert fuse holder 2020 in
the 753 Suppressor Module so that the corresponding mains
voltage range can be read normally and the adjacent white
arrow points to the white bar imprinted below the fuse holder
(see Fig. 4).

2.2 Mains connection
753 Suppressor Module / 8.753.1003 Instructions for Use 9
1313
220 -- 240 V
100 -- 120 V
1919
2020
220 -- 240 V
100 -- 120 V
Fig. 4: Setting the mains voltage
2.2.2 Fuses
One of the two fuses 0.5 A/slow-blow for 100…120 V or 0.25 A/slow-
blow for 220…240 V is installed in fuse holder 2020 of the 753 Suppressor
Module as standard.
Ensure that the instrument is never put into operation with fuses of
another type, otherwise there is danger of fire!
For checking or changing fuses, proceed as described in section 2.2.1.
2.2.3 Mains cable and mains connection
Mains cable
The instrument is supplied with one of three mains cables:
• 6.2122.020 with plug SEV 12 (Switzerland, …)
• 6.2122.040 with plug CEE(7), VII (Germany, …)
• 6.2133.070 with plug NEMA 5-15 (USA, …)
which are three-cored and fitted with a plug with an earthing pin. If a
different plug has to be fitted, the yellow/green lead (IEC standard)
must be connected to protective earth (protection class I).
Any break in the earthing inside or outside the instrument can make it
a hazard!
Mains connection
Plug the mains cable into mains connection plug 1919 of the 753 Sup-
pressor Module (see Fig. 4).
2.2.4 Switching the instrument on/off
The 753 Suppressor Module is switched on and off using mains switch
1313. When the instrument is switched on lamp 11 lights up.
100 – 120 V220 – 240 V
1313 Mains switch
1919 Mains connec-
tion plug
2020 Fuse holder

2 Installation
753 Suppressor Module / 8.753.1003 Instructions for Use
10
2.3 Connection to the 690 Ion Chromatograph
2.3.1 Electrical connection
In order to allow remote operation of the suppressor by the 690 Ion
Chromatograph, connection 1717 "Suppressor" of the 753 Suppressor
Module must be connected to the "Integrator Start" connection of the
690 Ion Chromatograph with the 6.2128.090 cable as shown in Fig. 5.
Additionally switch 66 <REMOTE> on the front of the 753 Suppressor
Module must be switched to "ON".
6.2128.090
753
690.0020/30
Fig. 5:Connection 753 Suppressor Module –
690 Ion Chromatograph
2.3.2 Connection of the suppressor block
The 1.753.0100 suppressor block must first be inserted in the 690 Ion
Chromatograph and connected to the 753 Suppressor Module. The in-
let and outlet capillaries mounted on the suppressor are then con-
nected up. Proceed as follows:
1Insert suppressor block
• Push lifting platform 5252 together with the fixed detector block
5454 located inside the 690 Ion Chromatograph down as far as
it will go.
• Place suppressor block 2121 on detector block 5454 in such a
way that suppressor connection 2222 points in the direction of
the door.
Cable of suppressor block

2.3 Connection to the 690 Ion Chromatograph
753 Suppressor Module / 8.753.1003 Instructions for Use 11
2Connect suppressor block
• Remove the plastic stopper from opening 1717 on the rear wall
of the 690 Ion Chromatograph and push cable 2323 mounted
on the suppressor block 2121 through this opening.
• Connect cable 2323 to connection 1717 “Suppressor“ of the 753
Suppressor Module (see Fig. 5).
3Connect column to injector
• Connect separating column 6363 to capillary connection 6565
leading to the injector according to section 3.7 of the 690 In-
structions for Use.
4Rinse column
• Rinse separating column 6363 with eluent for about 10 min
according to section 3.7 of the 690 Instructions for Use.
5Connect column to suppressor
• Screw inlet capillary 2424 marked with "Eluent" at suppressor
connection 2222 (see Fig. 6) to outlet end of separating column
6363 using a 6.2744.010 compression fitting.
6Connect suppressor to detector block
• Screw outlet capillary 2929 marked with "Detector" at suppres-
sor connection 2222 to a 6.2620.060 coupling using a
6.2744.010 compression fitting.
• Screw inlet capillary 5959 of detector block 5454 to the other end
of the 6.2620.060 coupling.
1
3
2
2424
2727
2828
29292525
2626
Fig. 6:Connections at suppressor
2424 Suppressor inlet
capillary for eluent
2525 Suppressor inlet
capillary for H2SO4
2626 Suppressor outlet
capillary for H2SO4
2727 Suppressor outlet
capillary for H2O
2828 Suppressor inlet
capillary for H2O
2929 Suppressor outlet
capillary for eluent
H2O
H2SO4
Waste
Eluent
Detector
Waste

2 Installation
753 Suppressor Module / 8.753.1003 Instructions for Use
12
7Attach pump tubings
• Loosen both tubing cartridges 44 from the holding clamp 99 by
pressing down snap-action lever 1010 and remove from
mounting pin 1212 on the 753 Suppressor Module (see Fig. 1).
• Press contact pressure lever 55 on both tubing cartridges
down as far as it will go.
• Insert a length of pump tubing 3333 (6.1826.050) into each of
the tubing cartridges as shown in Fig. 7. The white-yellow
stopper 3434 must click into the corresponding holder on the
left-hand side of the tubing cartridge.
• Place the tubing cartridges on mounting pin 1212 and press
down on the right-hand side until snap-action lever 1010 clicks
into position on holding clamp 99. Take care that no kinks are
formed in the pump tubing.
8Suppressor connection 2: H2SO4
• Pull the inlet capillary 2525 marked with “H2SO4“ at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 2929 of the 690 Ion Chromatograph or through the
door gap in the right-hand lower corner of the interior door
1313.
• Mount a PEEK compression fitting 3131 (6.2744.010) on the
end of inlet capillary 2525 and screw this on to coupling 3232
(6.2744.030) (see Fig. 7).
• Push coupling 3232 on to the outlet end of the first pump tubing
3333 (see Fig. 7).
• Mount suction tubing: cut a piece of PTFE Tubing 3030
(6.1803.020) to the desired length.
• Mount a PEEK compression fitting 3131 (6.2744.010) on one
end of PTFE tubing 3030 and screw this on to coupling 3232 (see
Fig. 7).
• Push coupling 3232 on to the inlet end of the first pump tubing
3333 (see Fig. 7).
• Immerse the other end of the suction tubing in a vessel
containing regeneration solution (normally 20 mmol/L H2SO4)
and fix in place.
• Pull outlet capillary 2626 marked with "Waste" at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 2929 of the 690 Ion Chromatograph.
• Lead outlet capillary 2626 to a sufficiently large waste container
and fix in place.

2.3 Connection to the 690 Ion Chromatograph
753 Suppressor Module / 8.753.1003 Instructions for Use 13
3030 3232 3333 3434 44 55 3434 3232 25/2825/281010 31313131
Fig. 7: Installing pump tubings
44 Tubing cartridge 3030 PTFE tubing (6.1803.020)
55 Contact pressure lever 3131 PEEK compression fitting
(6.2744.010)
1010 Snap-action lever 3232 Coupling (6.2744.030)
2525 Suppressor inlet capillary
for H2SO4
3333 Pump tubing (6.1826.050)
2828 Suppressor inlet capillary
for H2O3434 Stopper (white-yellow)
9Suppressor connection 3: H2O
• Pull the inlet capillary 2828 marked with “H2O“ at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 2929 of the 690 Ion Chromatograph or through the
door gap in the right-hand lower corner of the interior door
1313.
• Mount a PEEK compression fitting 3131 (6.2744.010) on the
end of inlet capillary 2828 and screw this on to coupling 3232
(6.2744.030) (see Fig. 7).
• Push coupling 3232 on to the outlet end of the second pump
tubing 3333 (see Fig. 7).
• Mount suction tubing: cut a piece of PTFE Tubing 3030
(6.1803.020) to the desired length.
• Mount a PEEK compression fitting 3131 (6.2744.010) on one
end of PTFE tubing 3030 and screw this on to coupling 3232 (see
Fig. 7).
• Push coupling 3232 on to the inlet end of the second pump
tubing 3333 (see Fig. 7).
• Immerse the other end of the suction tubing in a vessel
containing rinsing solution (normally dist. H2O) and fix in
place.

2 Installation
753 Suppressor Module / 8.753.1003 Instructions for Use
14
• Pull outlet capillary 2727 marked with "Waste" at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 2929 of the 690 Ion Chromatograph.
• Lead outlet capillary 2727 to a sufficiently large waste container
and fix in place.
10 Startup of 753 Suppressor Module
• Set switch <REMOTE> 22 on 753 Suppressor Module to
"OFF".
• Switch on 753 Suppressor Module with mains switch 1313 and
set switch <PUMP> 33 to "ON".
• Adjust the contact pressure for both tubing cartridges: press
contact pressure lever 55 upwards until the solutions just start
to be drawn in. Then press the contact pressure lever up-
wards until it clicks once more to obtain optimal contact pres-
sure.
• Before switching the suppressor to the next position for the
first time rinse all three suppressor units with solution for
5min.
• Check all tubing from the storage vessels through the tubing
cartridges and the suppressor up to the waste containers for
leakage of liquids. If liquid escapes anywhere the corre-
sponding compression fitting must be tightened further or
changed.
Pump tubings are consumable material with a lifetime which depends
on the contact pressure. This is why the tubing cartridges should be
raised completely by loosening snap-action lever 1010 on the right-hand
side if the pump is to remain switched off for a considerable length of
time (the set contact pressure remains unchanged).

2.4 Connection to the 732/733 IC system
753 Suppressor Module / 8.753.1003 Instructions for Use 15
2.4 Connection to the 732/733 IC system
2.4.1 Electrical connection
In order to allow remote operation of the suppressor and pump via the
732 IC Detector, connection 1717 of the 753 Suppressor Module must be
connected to the "Remote" connection of the 732 IC Detector by means
of the 6.2143.210 cable (available as an option) according to Fig. 8.
Both switches 22 and 66 <REMOTE> on the front of the 753 Suppressor
Module must additionally be set to "ON" to permit remote operation.
753
Fig. 8:Connection 753 Suppressor Module –
732/733 IC system
2.4.2 Connection of the suppressor block
The 1.753.0100 suppressor block must first be inserted in the 733 IC
Separation Center and connected to the 753 Suppressor Module. The
inlet and outlet capillaries mounted on the suppressor are then con-
nected up. Proceed as follows:
6.2143.210
Cable of
suppressor block

2 Installation
753 Suppressor Module / 8.753.1003 Instructions for Use
16
1Insert suppressor block
• 733.0010 IC Separation Center (1-channel system):
Place suppressor block 2121 to the right-hand side of detector
block 7777 (see Fig. 14 of 732/733 Manual) on the floor of the
inner chamber in such a way that suppressor connection 2222
points in the direction of the door.
• 733.0020 IC Separation Center (two-channel system):
Place suppressor block 2121 on the floor of the inner chamber
(either instead of a pulsation damper 6767 or in front of both
pulsation dampers, see Fig. 14 of 732/733 Manual).
2Connect suppressor block
• 733.0010 IC Separation Center (one-channel system):
Remove the plastic stopper from opening 3131 on the rear
panel of IC Separation Center 733 and push cable 2323
mounted on the suppressor block 2121 through this opening.
• 733.0020 IC Separation Center (two-channel system):
Remove the plastic stopper from opening 4141 or 4343 on the
733 IC Separation Center and push cable 2323 mounted on the
suppressor block 2121 through the opening.
• Connect cable 2323 to connection 1717 “Suppressor“ of the 753
Suppressor Module (see Fig. 8).
3Connect column to injector
• see section 2.8.6 of 732/733 Manual
4Rinse column
• see section 2.8.6 of 732/733 Manual
5Connect column to suppressor
• Screw inlet capillary 2424 marked with "Eluent" at suppressor
connection 2222 (see Fig. 6) to outlet end of separating column
7272 using a 6.2744.010 compression fitting.
6Fix column
• Insert one or two column holders 8080 (6.2027.030, 6.2027.040
or 6.2027.050) in mounting rails 7979 and fasten separating
column in the column holder.
7Connect suppressor to detector block
• Screw outlet capillary 2929 marked with "Detector" at suppres-
sor connection 2222 to coupling 9292 (6.2620.060) using a
6.2744.010 compression fitting.
• Screw inlet capillary 7878 of detector block 7777 to the other end
of the coupling 9292.

2.4 Connection to the 732/733 IC system
753 Suppressor Module / 8.753.1003 Instructions for Use 17
8Fix connection suppressor – detector block
• Insert one of the column holders 8080 (6.2027.030, 6.2027.040
or 6.2027.050) in mounting rail 7979 and fasten coupling 9292 in
the column holder.
9Attach pump tubings
• Loosen both tubing cartridges 44 from the holding clamp 99 by
pressing down snap-action lever 1010 and remove from
mounting pin 1212 on 753 Suppressor Module (see Fig. 1).
• Press contact pressure lever 55 on both tubing cartridges
down as far as it will go.
• Insert a length of pump tubing 3333 (6.1826.050) into each of
the tubing cartridges as shown in Fig. 7. The white-yellow
stopper 3434 must click into the corresponding holder on the
left-hand side of the tubing cartridge.
• Place the tubing cartridges on mounting pin 1212 and press
down on the right-hand side until snap-action lever 1010 clicks
into position on holding clamp 99. Take care that no kinks are
formed in the pump tubing.
10 Suppressor connection 2: H2SO4
• Pull the inlet capillary 2525 marked with “H2SO4“ at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 4141 or 4343 of the 733 IC Separation Center.
• Mount a PEEK compression fitting 3131 (6.2744.010) on the
end of inlet capillary 2525 and screw this on to coupling 3232
(6.2744.030) (see Fig. 7).
• Push coupling 3232 on to the outlet end of the first pump tubing
3333 (see Fig. 7).
• Mount suction tubing: cut a piece of PTFE Tubing 3030
(6.1803.020) to the desired length.
• Mount a PEEK compression fitting 3131 (6.2744.010) on one
end of PTFE tubing 3030 and screw this on to coupling 3232 (see
Fig. 7).
• Push coupling 3232 on to the inlet end of the first pump tubing
3333 (see Fig. 7).
• Immerse the other end of the suction tubing in a vessel
containing regeneration solution (normally 20 mmol/L H2SO4)
and fix in place.
• Pull outlet capillary 2626 marked with "Waste" at suppressor
connection 2222 (see Fig. 6) through one of the rear panel
openings 4141 or 4343 of the 733 IC Separation Center.
• Lead outlet capillary 2626 to a sufficiently large waste container
and fix in place.