Becton, Dickinson and Company BD Rhapsody Express Product manual

Doc ID: 76955 Rev. 2.0
08/2018
Becton, Dickinson and Company
BD Biosciences
2350 Qume Drive San Jose, CA 95131 USA
Tel [phone], prompt 2, 2
[e-mail]
BD Rhapsody™ Express
Single-Cell Analysis System
Installation and Maintenance
Guide
For the BD Rhapsody™ Express instrument

Doc ID: 76955 Rev. 2.0
Copyrights/trademarks
Trademarks are the property of their respective owners.
© 2018 BD. BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company.
The information in this guide is subject to change without notice. BD Biosciences reserves the right to change
its products and services at any time to incorporate the latest technological developments. Although this guide
has been prepared with every precaution to ensure accuracy, BD Biosciences assumes no liability for any errors
or omissions, nor for any damages resulting from the application or use of this information. BD Biosciences
welcomes customer input on corrections and suggestions for improvement.
Regulatory information
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Patents
The BD Rhapsody™ Targeted mRNA and AbSeq Reagent Kit is covered by one or more of the following U.S.
patents: 8835358, 9315857, 9290808, 9290809, 9567646, and 9598736.
Revision history
Revision Date Change made
Doc ID: 76955 Rev. 1.0 02/2018 Initial release
Doc ID: 76955 Rev. 2.0 08/2018 —Changed the name of “BD Rhapsody™
Sample Loading Station” to “BD Rhapsody™
Express instrument.”
—Updated cross-references to “BD
Rhapsody™ Express Single-Cell Analysis
System Instrument User Guide (Doc ID:
214063).”

Doc ID: 76955 Rev. 2.0
Contents
Chapter 1: Introduction 5
About this guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Safety symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Safety data sheets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Instrument technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Chapter 2: Installation 11
BD Rhapsody™ Express instrument installation . . . . . . . . . . . . . . . . . . . . . . . . . 12
Chapter 3: Maintenance 15
Maintaining the BD Rhapsody™ Express instrument . . . . . . . . . . . . . . . . . . . . . 16
Returning the BD Rhapsody Express instrument for service . . . . . . . . . . . . . . . . 20
Maintaining the BD Rhapsody™ P1200M and P5000M pipettes . . . . . . . . . . . . 20
Returning the BD Rhapsody P1200M and P5000M pipettes for service . . . . . . . 21
Chapter 4: Troubleshooting 23
BD Rhapsody™ Express instrument troubleshooting . . . . . . . . . . . . . . . . . . . . . 24
Appendix A: Safety 27
General safety and limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Chemical safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Waste disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Glossary 29

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1
Introduction

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide6
About this guide
Introduction This guide provides instructions on installing and maintaining the
BD Rhapsody™ Express instrument.
Intended use This guide is intended for users of the BD Rhapsody Express
instrument. Read this guide prior to installing and maintaining the
instruments.
For intended use of the BD Rhapsody™ Single-Cell Analysis
system, see the BD Rhapsody™ Express Single-Cell Analysis
System Instrument User Guide (Doc ID: 214063).
For detailed site requirements and technical specifications, see the
BD Rhapsody™ Express Single-Cell Analysis System Site
Preparation Guide (Doc ID: 75750).
Genomics technical publications are available for download from
the BD Genomics Resource Library at bd.com/genomics-resources.
Safety symbols
Introduction This topic describes the safety symbols used in this guide.
Safety symbols The following table lists the safety symbols used in this guide to
alert you to potential hazards.

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Chapter 1: Introduction 7
Symbol Meaning
Caution. Indicates the need for the user to consult the
instructions for use for important cautionary information,
such as warnings and precautions that cannot, for a variety
of reasons, be presented on the device itself.
Biological hazard. All surfaces that come in contact with
biological specimens can transmit potentially fatal disease.
Use universal precautions when cleaning surfaces. Wear
suitable protective clothing, eyewear, and gloves.
Lifting hazard

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide8
Safety data sheets
Introduction This topic describes how to obtain safety data sheets (SDSs).
Obtaining SDSs Before handling chemicals, read and understand the SDSs. To
obtain SDSs for chemicals ordered from BD Biosciences, contact
BD Biosciences technical support at [email protected].
Instrument technical support
Introduction This topic describes how to get technical support for the
BD Rhapsody Express instrument.
Contacting
technical support
If technical assistance is required, contact
[e-mail] or 1.877.232.8995, prompt 2, 2 or
+353.61.307734. You can contact technical support in Europe at
help.bio[email protected]d.com or at these telephone numbers:
Location Telephone number Location Telephone number
Worldwide [phone] —
Austria 01 92 80 465 Netherlands 010 71 14 800
Belgium 02 40 17 093 Norway 800 18 530
Denmark 80 88 21 93 Portugal 800 86 01 76
Finland 800 11 63 17 South
Africa
0800 98 10 08
France 01 70 70 81 93 Spain 91 41 46 250

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Chapter 1: Introduction 9
Before contacting BD Biosciences, have the following information
available:
• Product name, model number, and serial number on the rear of
the instrument.
• Details of recent system performance
Germany 069 22 22 25 60 Sweden 08 50 69 21 54
Greece 00800 12 75 06 Switzerland 044 58 04 373
Italy 02 36 00 36 85 United
Kingdom
0207 07 53 226
Location
(continued)
Telephone number Location Telephone number

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2
Installation

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide12
BD Rhapsody™ Express instrument installation
Preparing the site Prepare the site for the Express instrument. See the
BD Rhapsody™ Express Single-Cell Analysis System Site
Preparation Guide (Doc ID: 75750).
Installation Contact BD Biosciences technical support at
[e-mail].
Lifting the BD
Rhapsody Express
instrument
Lift the BD Rhapsody Express instrument by firmly grasping the
cutout on each side of the instrument:

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Chapter 2: Installation 13
Installing the drip
tray and splash
shield
1. Move the front slider to OPEN:
2. Review the location of parts:
OPEN WASTE BEADS
Insert drip tray here
Drip tray Splash guard

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide14
3. Orient the white drip tray so that its larger compartment faces
upwards and is positioned towards the interior of the
instrument. The compartments of the drip tray correspond to
the tube sizes above it.
4. Insert the drip tray fully until it stops.
5. Move front slider to BEADS:
6. Slide the splash shield fully, underneath the drip tray, until it
stops.
OPEN WASTE BEADS

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3
Maintenance

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide16
Maintaining the BD Rhapsody™ Express
instrument
Introduction This section describes how to maintain and decontaminate the
BD Rhapsody Express instrument.
General
maintenance
guidelines
Use 10% (v/v) bleach, 70% (v/v) ethyl alcohol, or 70% (v/v)
isopropyl alcohol to clean the BD Rhapsody Express instrument.
Cleaning the drip
tray and tube
receptacles
1. During cleaning, wear suitable protective clothing, eyewear,
and gloves.
2. After each use, move the front slider to OPEN:
OPEN WASTE BEADS

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Chapter 3: Maintenance 17
3. Remove the white drip tray (red oval) by lifting it slightly and
pulling it out:
4. If necessary, appropriately dispose of the liquids in the drip
tray.
5. Use pre-moistened cleaning wipes with 10% bleach or 70%
ethyl alcohol to wipe the sides, top, and bottom of the drip
tray and the tube receptacles for the Waste Collection
Container (Cat. No. 650000090) and Bead Retrieval Tube
(5 mL LoBind Tube).
6. Use a dry lint-free wiper to dry the components.
7. Orient the white drip tray so that its larger compartment faces
upwards and is positioned towards the interior of the
instrument, and then reinsert the drip tray fully until it stops.
The compartments of the drip tray correspond to the tube sizes
above it.
8. Move the front slider with the correct collection tubes to the
appropriate position to BEADS or WASTE.
9. Dispose of the used cleaning materials in the biohazard
containers.

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide18
Cleaning the splash
shield
1. Move the front slider to BEADS for access to the splash shield
tab:
OPEN WASTE BEADS

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Chapter 3: Maintenance 19
2. On the left side of the BD Rhapsody Express instrument, grasp
the bottom tab, and pull out the splash shield (red oval). To
avoid spills, do not tilt the tray:
3. Appropriately dispose of liquid in the splash shield.
4. Wash the splash shield with 10% bleach or 70% ethyl alcohol.
5. Use a dry lint-free wiper to dry the splash shield.
6. Slide the splash shield fully into the left opening of the
instrument until it stops.
7. Dispose of the used cleaning materials in biohazard containers.
Cleaning the
cartridge tray
1. Remove the BD Rhapsody™ Cartridge, if present.
2. Use a dry lint-free wiper to dry the cartridge tray, and then use
a lint-free wiper moistened with 10% bleach or 70% ethyl
alcohol to clean the tray.
3. Dispose of the used cleaning materials in biohazard containers.
Tab

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BD Rhapsody Express Single-Cell Analysis System Installation and Maintenance Guide20
Returning the BD Rhapsody Express instrument
for service
1. Before returning the BD Rhapsody Express instrument to
BD Biosciences, follow the cleaning protocol. See Maintaining
the BD Rhapsody™ Express instrument (page 16).
2. Complete the decontamination label provided by
BD Biosciences.
3. Contact BD Biosciences at [e-mail] for
return authorization.
4. Complete the return shipping label.
5. Ship the BD Rhapsody Express instrument to BD Biosciences
according to shipping instructions on the label.
Maintaining the BD Rhapsody™ P1200M and
P5000M pipettes
Introduction This section describes how to maintain and decontaminate the
BD Rhapsody P1200M and P5000M pipettes.
General
maintenance
guidelines
• Wipe the pipettes with pre-moistened cleaning wipes
containing 70% (v/v) ethyl alcohol or 70% (v/v) isopropyl
alcohol.
• Charge the pipettes between experiments.