Becton dickinson Sero-fuge 2000 series User manual

SERO-FUGE®

2000

Series

Centrifuges

Operator's

Manual

OPERATORS

MAL.

.

REF./RIF./

BEST.NR.:

SERO

2608

42035101

R/P

QTY.

1

BECTON

SERO-FUGE?

2000

Series

Centrifuges

Operator's

Manual

CAUTION

A

Potential

hazards

exist.

Read

and

understand

this

manual

thoroughly

before

using

equipment.

If

the

equipment

is

used

in

a

manner

not

specified,

the

protection

provided

by

the

equipment

may

be

impaired.

DANGER

Explosion

Hazard

Do

not

use

this

equipment

in

the

presence

of

flammable

anesthetics,

where

explosive

materials

are

used,

or

with

materials

which

chemically

interact

vigorously.

BECTON

DICKINSON

PRIMARY

CARE

DIAGNOSTICS

BECTON

DICKINSON

AND

COMPANY

7

Loveton

Circle

Sparks,

Maryland

21152-0370

Я

1-800-631-8064

CONTENTS

Section

1

—

Introduction

Symbol

Definitions

…

Notes,

Cautions,

and

Section

2

—

Intended

Use

|

(DOVBIVIBWI

ciao

SERO-FUGE®

2001

ЗЕВО-РИСЕ®

2002

._....................-

онлаин

|

Use

of

the

SERO-FUGE®

2000

Series

Centrifuges

È

Agglutination

Tests..

Incubation........

Cell

Washing

...

Section

3

-

Operating

Procedures

....

Installation

Site

Preparation

..

ROOS

3

Load

Balancing

Applying

Power

Rotors

and

Accessories

..

ROSS

tcicncaa

Accessories

..

Line

Cords

Manuals

...

PowerReguiremenis.............................................

00000

늬

10

SERO-FUGE®

2001

(115

VAC,50-60

Hz)

Са09

№:

420351

................

ο

ος

10

SERO-FUGE®

2001

(230

VAC,

50-60

Hz)

Са169

№0420354...........

тульское

аня

10

SERO-FUGE®

2002

(115

VAC,

50-60

Hz)

Catalog

No.

420352

Performance

and

Specifications..

Speeds

115

VAC

SERO-FUGE®

2000

Series

Centrifuges.

|

230

VAC

SERO-FUGE®

2000

Series

Centrifuges.

|

Timer

Specifications...

o

Centrifuge

Weights

an

ensio

Operating

Clearance

Envelope

..

Environmental

Specifications

..

Operating

Precautions...

Controls

and

Indicators..

iii

OPERATOR'S

MANUAL

Speed

Verification....

Cleanliness.....

Operator

Training..

Hazards.

Section

4—

Maintenance

and

Service

Cleaning

and

Decontamination

of

Spills..

Periodic

Inspection...

a

Fuse

Replacement...

Fuse

Replacement

for

115

VAC

SERO-FUGE®

2001/2002.

Fuse

Replacement

for

230

VAC

SERO-FUGE®

2001...

Transportation

Section

5—

SERO-FUGE“

2000

Series

Centrifuge

Warranty

.......

26

AppendixA-ServiceCenters...............................................

28

ーー

2000

SERIES

CENTRIFUGES

SECTION

1

—

INTRODUCTION

II

This

manual

provides

operating

instructions

for

the

Clay

Adams®

2000

Series

Centrifuges

—

the

single

speed

2001

and

the

two

speed

2002.

Symbol

Definitions

The

following

is

an

explanation

of

the

symbols

found

throughout

the

manual

and

located

on

the

centrifuge

units.

A

si

x

CAUTION

—

Risk

of

Personal

Injury

This

symbol

is

used

in

both

Caution

and

Warning

messages

to

attract

the

user's

attention

to

potential

hazards

when

using

the

centrifuge.

CAUTION

—

Risk

of

Electrical

Shock

This

symbol

is

shown

on

the

instrument

to

warn

the

user

of

possible

electrical

hazards.

DANGER

-

Explosion

Hazard

Do

not

use

in

the

presence

of

flammable

anesthetics

where

explosive

materials

are

used

or

with

materials

which

chemically

interact

vigorously.

Notes,

Cautions,

and

Warnings

The

following

is

an

explanation

of

Note,

Caution,

and

Warning

boxes

contained

throughout

this

manual.

Important

information

about

system

use

worthy

of

special

NOTE

attention.

Information

on

an

activity

that

potentially

could

cause

damage

to

the

system.

CAUTION

A

OPERATOR’S

MANUAL

WARNING

A

Information

on

an

activity

which

potentially

could

cause

injury

to

the

user.

SECTION

2

—

INTENDED

USE

Overview

SERO-FUGE®

2001

and

SERO-FUGE

2002

Centrifuges

are

compact,

highly

versatile

machines

for

use

in

blood

banks

and

clinical

laboratories.

They

are

specifically

designed

to

simplify

many

basic

test

procedures,

such

as

blood

typing,

manual

cell

washing,

cross-matching,

genotyping,

Coombs

testing,

and

Anti-Rh

titers.

Both

models

feature

a

lid

safety

latch

that

performs

the

following

functions:

+

Prevents

the

unit

from

spinning

while

the

lid

is

open

+

Automatically

locks

the

lid

when

the

lid

is

closed

+

Prevents

lid

opening

until

the

rotor

has

stopped

spinning

Both

models

allow

for

complete

manual

control

by

the

operator

over

the

time

of

the

spin

cycle.

SERO-FUGE®

2001

The

SERO-FUGE®

2001

is

a

single

speed

machine

designed

to

minimize

centrifugation

time

in

the

laboratory.

It

incorporates

the

following

features:

+

A

safety

lid

lock

that

automatically

locks

when

the

lid

is

closed

+

Anend

of

cycle

alarm

to

inform

the

operator

that

the

cycle

is

completed

+ A

solid

state

digital

timer

providing

precise

cycle

times

|

H

i

v

O

er

as

co

2000

SERIES

CENTRIFUGES

+

A

dynamic

brake

to

shorten

the

rotor

deceleration

time

after

a

completed

cycle.

SERO-FUGE®

2002

The

SERO-FUGE

2002

is

a

dual

speed

machine.

The

low

speed

setting

is

useful

when

reagent

manufacturers

recommend

low

speed

spins.

This

unit

incorporates

the

same

features

as

the

SERO-FUGE

2001.

This

unit

is

not

available

for

use

at

230

VAC.

Use

of

the

SERO-FUGE"

2000

Series

Centrifuges

SERO-FUGE

2000

Series

Centrifuges

have been

designed

to

facilitate

blood

testing

procedures

involving

centrifugation,

incubation, and

cell

washing,

with

a

minimum

of

tube

handling.

Many

procedures

can

be

performed

entirely

without

removing

tubes

from

the

centrifuge

rotor,

thus

reducing

the

possibility

of

errors

in

transferring

tubes.

Tubes

can

be

centrifuged

and

incubated

without

removal

from

the

rotor.

The

following

notes

on

use

of

the

SERO-FUGE

2000

Series

Centrifuges

in

the

more

common

test

procedures

are

intended

to

set

practical

guidelines

for

the

technologist.

Many

standard

texts

and

handbooks

provide

recommendations

regarding

time

periods

for

various

centrifugations.

AGGLUTINATION

TESTS

Whenever

possible,

it

is

important

that

the

anti-serum

manufacturer's

instructions

be

followed

on

recommended

time

periods

for

centrifuging

cells

with

their

sera

in

the

2000

Series

Centrifuges.

NOTE

It

is

recommended

that

the

optimum

centrifugation

period

be

determined

by

controlled

reactions

using

known

cells.

This

method

is

the

easiest

way

to

determine

the

time

necessary

to

produce

the

desired

tightness

of

agglutination.

INCUBATION

Many

blood

testing

procedures

require

incubation

at

37°C

before

and

after

centrifugation.

The

SERO-FUGE®

2000

Series

Centrifuge

rotors

OPERATOR’S

MANUAL

have

been

designated

to

allow

the

incubation

of

these

reactions

without

removing

the

tubes

from

the

rotor.

You

may

lower

the

entire

rotor

into

a

water

bath

for

the

test

being

performed.

When

the

rotor

is

removed,

water

will

drain

through

the

perforations

in

its

base.

Allow

the

water

to

drain

completely

prior

to

re-centrifugation

or

transporting

the

tubes.

CELL

WASHING

The

12-place Centrifuge

Rotor,

Catalog

No.

420545, may

be

conveniently

used

for

many

tests

that

require

single

or

multiple

blood

cell

washings.

Because

the

tubes

are

maintained

at

an

angle

of

45°

during

centrifugation,

the

centrifuge

is

highly

efficient

in

washing

cells.

The

cells

are

deposited

quickly

after

sliding

down

the

angled

tubes.

After

the

button

is

formed,

the

supernatant

solution

can

be readily

poured

off

all

tubes

simultaneously.

This

is

done

by

removing

the

rotor

from

the

drive

spindle

immediately

after

it

stops

spinning,

placing

the

retainer

ring

around

the

tubes,

and

inverting

the

rotor.

SECTION

3

—

OPERATING

PROCEDURES

The

following

paragraphs

describe

Installation,

Load

Balancing,

Controls

and

Indicators,

and

Operation

of

the

SERO-FUGE

2000

Series

Centrifuges.

WARNING

A

“UNIVERSAL

PRECAUTIONS”'

SHOULD

BE

FOLLOWED

IN

HANDLING

ALL

ITEMS

CONTAMINATED

WITH

BLOOD

OR

OTHER

BODY

FLUIDS.

‘Recommendations

for

Prevention

of

HIV

Transmission

in

Health

Care

Settings.

MMWR

1987;

36

(Supplement

#2S):

(Inclusive

Page

Numbers).

KER

2000

SERIES

CENTRIFUGES

installation

SITE

PREPARATION

Place

the

centrifuge

on

a

clean

level

surface.

The

user

should

mark

a

boundary

no

less

than

300mm

(11.8”)

around

the

centrifuge

and

ensure

that

no

person

or

any

hazardous

materials

are

within

the

boundary

while

the

centrifuge

is

operating.

The

centrifuge

should

be

located

so

that

the

ambient

air

can

readily

circulate

around

the

unit.

The

SERO-FUGE®

2000

Series

Centrifuge

is

a

table

top

unit

and

need

not

be

secured

to

the

table.

ROTORS

The

SERO-FUGE

2000

Series

Centrifuges

are

shipped

fully

assembled

and

ready

for

use.

Before

operating

the

machine,

the

desired

rotor

must

be

installed

and locked

into

position.

A

12-place

rotor

is

shipped

with

the

unit.

This

rotor

is

interchangeable

with

8

and

6-place

rotors

which

are

also

available

for

use

with

the

SERO-FUGE

2000

Series

Centrifuges.

All

rotors

are

installed

in

an

identical

manner

by

rotating

them

on

the

drive

spindle

until

the

rotor

drops

over

the

and

locks

into

place.

NOTE

Inspect

all

rotors

regularly

for

integrity

and

discontinue

use

if

cracks

or

other signs

of

damage

are

visible.

OPERATOR’S

MANUAL

LOAD

BALANCING

For

smooth

centrifuge

operation

and

extended

equipment

life,

it

is

essential

that

loads

be

balanced

as

equally

as

possible.

Use

of

a

tube

balance

is

recommended

for

best

results.

CAUTION

A

Before

operating

any

centrifuge,

the

load

must

be

balanced.

Never

attempt

to

balance

the

unit

by

adding

weights,

mercury,

or

shot

to

the

bottom

of

a

tube.

APPLYING

POWER

To

apply

power

to

the

centrifuge,

simply

plug

the

unit into

the

proper

AC

receptacle.

See

the

section

below

—

Power

Specifications

—

for

power

requirements

and

precautions.

CAUTION

A

It

is

recommended

that

the

centrifuge

be

wired

to

a

remote

emergency

cutoff

switch

so

power

can

be

disconnected

from

outside

the

room.

2000

SERIES

CENTRIFUGES

Rotors

and

Accessories

ROTORS

SERO-FUGE®

2000

Series

Centrifuges

can use three

types

of

rotors.

They

are

as

follows:

12-Place

Rotor,

Catalog

No.

420545

The

12-place

rotor

can

accommodate

the

following

tubes:

75

mm

x

13

mm

without

rubber

stopper

75

mm

x

10

mm

(3”

x

3/8”)

Sero-Tubes

75

mm

x

12

mm

(3”

x

1/2”)

Kahn

Tubes

(6)

75

mm

x

13

mm

tubes

with

rubber

stopper

+ + + +

Any

number

of

test

tubes

(except

one,

five,

seven

or

eleven)

can

be

centrifuged

without

unbalancing

the

rotor,

provided

they

are

placed

symmetrically

about

the

spindle.

Tubes

swing

out

to

an

angle

of

45°

when

the

rotor

is

spinning

and

return

to

a

vertical

position

at

rest.

A

retaining

ring

is

provided

in

the

rotor

to

hold

the

tubes

firmly

in

place.

This

enables

the rotor

to

be

inverted

to

pour

off

supernatant

liquid.

8-Place

Rotor,

Catalog

No.

420224

The

8-place

rotor

can

accommodate

the

following

tubes:

+

75mm

x

10mm

(3”

x

3/8”)

Sero-Tubes

75

mm

x

12

mm

(3”

x

1/2”)

Kahn

Tubes

64

mm

x

10.25

mm

tubes

with

rubber

stopper

82

mm

x

10.25

mm

tubes

with

rubber

stopper

103

mm

x

10.25

mm

tubes

with

rubber

stopper

75

mm

x

13

mm

tubes

with

or

without

rubber

stopper

+ + +

+

+ è

75

mm

x

13

mm

Vacutainer®

Blood

Collection

Tubes

with

Hemogard®

closures

+

100

mm

x

13

mm

tubes

with

or

without

rubber

stopper

100

mm

x

13

mm

Vacutainer®

Blood

Collection

Tubes

with

Hemogard®

Closures

The

rotor

has

a

fixed

angle

of

45°.

OPERATOR

'S

MANUAL

6-Place

Rotor,

Catalog

No.

420526

The

6-place

rotor

can

accommodate

the

following

tubes:

100

mm

x

13

mm

glass

with

rubber

stopper

103

mm

x

10.25

mm

glass

with

rubber

stopper

100

mm

x

16

mm

glass

with

rubber

stopper

+ + + +

100

mm

x

16

mm

Vacutainer®

Blood

Collection

Tubes

with

rubber

stopper

(Serum

Separation

Tubes)

The

rotor

has a

fixed

angle

of

45°.

WARNING

A

USE

ONLY

ROTORS

MANUFACTURED

OR

SUPPLIED

BY

CLAY

ADAMS®

SPECIFICALLY

FOR

USE

IN

THE

SERO-FUGE®

2000

SERIES

CENTRIFUGES.

USE

OF

OTHER

ROTORS

MAY

BE

HAZARDOUS.

NOTE

Each

of

the

SERO-FUGE

rotors

is

rated

for

safe

operation

at

speeds

up

to

3600

RPM

with

the

tubes

fully

loaded

with

fluid

not

exceeding

a

density

of

1.10 g/cc

(i.e.,

whole

blood).

ACCESSORIES

Line

Cords

North

American

Line

Cord

(NEMA

5-15)

Catalog

No.

42432612

Commonly

used

in

the

United

States,

Canada,

Mexico,

Central

America, Colombia,

Venezuela,

Ecuador,

and

elsewhere.

2000

SERIES

CENTRIFUGES

British

Standard

(BS

1363)

with Integral

Fuse

Catalog

No.

42251015

Commonly

used

in

the

United

Kingdom,

Ireland,

Hong

Kong,

Malaysia,

Singapore,

and

elsewhere.

Continental

European

“Schuko”

(CEE

7/7)

Catalog

No.

42251012

Commonly

used

in

Germany,

Austria,

The

Netherlands,

Sweden,

Norway,

Finland,

and

elsewhere.

May

be

plugged

into

French

and

Belgian

receptacles.

Fuses

115VAC

SERO-FUGE®

2001/2002

REPLACEMENT

FUSES

Catalog

No.

42035102

UL

listed

and

CSA

certified

5mm

x

20mm,

Type

T,

time

lag—fuses

rated

at

4

amps,

125VAC.

230VAC

SERO-FUGE®

2001

Replacement

Fuses

Catalog

No.

42035103

SEMKO,

BSI,

or

VDE

approved

5mm

x

20mm,

Type

T,

time-lag

fuses

rated

at

3

amps,

250VAC.

MANUALS

SERO-FUGE®

2000

Series

Centrifuge

Operator's

Manual

Catalog

No.

42035101

OPERATOR'S

MANUAL

Power

Requirements

SERO-FUGE®

2001

(115

VAC,50-60

Hz)

CATALOG

No.

420351

Connect

to

a

3-wire

grounded

AC

receptacle

rated

at

115

volts,

50—

60

Hz.

The

centrifuge

will

operate

within

a

voltage

range

of

103

to

132

VAC.

SERO-FUGE®

2001

(230

VAC,

50-60

Hz)

CATALOG

No

420354

Connect

to

a

3-wire

grounded

AC

receptacle

rated

at

230

volts,

60

Hz.

The

centrifuge

will

operate

within

a

voltage

range

of

198

to

264

VAC.

SERO-FUGE®

2002

(115

VAC,

50-60

Hz)

CATALOG

No.

420352

Connect

to

a

3-wire

grounded

AC

receptacle

rated

at

115

volts,

either

50

or

60

Hz.

The

centrifuge

will

operate

within

a

voltage

range

of

103

to

132

VAC.

Performance

and

Specifications

SPEEDS*

Under

full

load:

12-,

8-,

or

6-place

rotor:

Speed

at

the

following

Electrical

Specifications:

MODEL

103

192

VAC

103-132

VAC

198

:

264

VAC

198-264

VAC

SERO-FUGE

2001

3100

-

3550

rpm

2550

-

2950

rpm

3100

-

3550

rpm

2550

-

2950

rpm

Ее

(High

| 3100-3550

rpm

|

2550-2950

rpm

МА МА

—

nos

(Low

|

2350-2450rpm

|

2350-2450

rpm

N/A

”Note:

Full

load

is

defined

as

any

SERO-FUGE

2000

Series

Centrifuge

rotor

loaded

to

capacity

with

any

tube

described

in

the

"Rotors

and

Accessories”

section,

filled

to

thier

maximum

volume

with

a

fluid

not

exceeding

a

density

of

1.10 g/cc

(e.g.,

whole

blood).

10

2000

SERIES

CENTRIFUGES

115

VAC

SERO-FUGE®

2000

CENTRIFUGES

Nominal

Line

Voltage:

115

VAC

Maximum

Line

Voltage:

132

VAC

*

Minimum

Line

Voltage:

103

VAC

*

Frequency:

50-60

Hz

Single

Cycle

Motor

Inrush

Current:

6.5

Amps

Steady

State

Current

(Motor

Running):

1.4

Amps

Idle

Current

(Motor

Not

Running):

0.05

Amp

Power

(Motor

Running):

66

Watts

Power

(Motor

Not

Running):

1.5

Watts

Thermal

Generation

Motor

Running:

175

BTUs/

Hr

Motor

Not

Running:

5

BTUs/

Hr

Motor

Braking

Time

(Rotor

Fully

Loaded):

30

Sec

*Note:

All

specifications

are

typical

with

the

exception

of

the

maximum

and

minimum

line

voltages.

230

VAC

SERO-FUGE®

2000

CENTRIFUGES

Nominal

Line

Voltage:

230

VAC

Maximum

Line

Voltage: 264

VAC

**

Minimum

Line

Voltage:

198

VAC

**

Frequency:

50-60

Hz

Single

Cycle

Motor

Inrush

Current

(230

VAC,

50

Hz):

4

Amps

Steady

State

Current

(Motor

Running)

(230

VAC,

50

Hz):

0.7

Amp

Idle

Current

(Motor

Not

Running)

(230

VAC,

50

Hz):

0.04

Amp

Power

(Motor

Running)

(230

VAC,

50

Hz):

78

Watts

Power

(Motor

Not

Running)

(230

VAC,

50

Hz):

3

Watts

Thermal

Generation

Motor

Running(230

VAC,

50

Hz):

212

BTUs/

Hr

Motor

Not

Running(230

VAC,

50

Hz):

10

BTUs/

Hr

Motor

Braking

Time

(Rotor

Fully

Loaded):

30

Sec

**Note:

All

specifications

are typical with

the

exception

of

the

maximum

and

minimum

line

voltages.

11

OPERATOR'S

MANUAL

TIMER

S

PECIFICATIONS

Maximum

Cycle Time:

99

minutes

and

99

seconds

Minimum

Cycle

Time:

1

second

Timer

Accuracy:

+1

second

/

30

minutes

CENTRIFUGE

WEIGHTS

AND

DIMENSIONS

Closed

unit,

both

models:

Width/Length/Height

απ

κο

κο”

Net

Weight

28

lbs

OPERATING

CLEARANCE

ENVELOPE

Piacement

of

the

centrifuge

must

include

a

clearance

envelope

of

no

less

than

300

mm.

ENVIRONMENTAL

SPECIFICATIONS

The

SERO-FUGE®

2000

Series

Centrifuges

are

designed

as

follows:

@

Indoor

Use

Only

Ambient

Room

Temperature

Range

of

5°C

—

40°C*

Relative

Humidity

of

20%

—

90%,

non-condensing

Elevations

Up

to

2000m

Above

Sea

Level

+ + +

è

IEC

664

Pollution

Degree

Becton Dickinson SERO-FUGE 2000 Series User manual

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