Benchmark Scientific Bioclave User manual

Operations Manual
PH: 908-769-5555 EM: [email protected]
WEB: www.BenchmarkScientific.com
For Research Use Only

Thank you for choosing the BioClave Benchtop Sterilizer.
Prior to operating this instrument, please read the operations manual
carefully and follow all installation instructions.
TABLE OF CONTENTS
Instructions manual
Instructions manual
1.GENERAL ------------------------------------------------------- 1
2.TECHNICAL SPECIFICATIONS --------------------------------- 2
3.PACKING CONTENT ------------------------------------------ 3
4.INSTALLATION ----------------------------------------------- 4
5.OPERATION ----------------------------------------------------- 5
5.1 SETUP -------------------------------------------------------- 5
5.2 PREPARATION OF STERILIZATION MATERIALS ----- 6
5.3 SELECTING THE STERILIZATION PROGRAM --------- 7
5.4 RUNNING THE STERILIZATION PROGRAM ------------ 8
6. ADVANCED SETTINGS --------------------------------------- 11
7. MAINTENANCE ----------------------------------------------- 15
8. TRANSPORT AND STORAGE ------------------------------- 18
9. ERROR CODES ------------------------------------------------ 19
10. SAFETY DEVICES ------------------------------------------- 20
1.WATER PROPERTIES/CHARACTERISTICS ---------- 21
2. DIAGRAMS OF THE STERILIZATION PROGRAMS ----- 22
3.WIRING DIAGRAMS ----------------------------------- 25
4. HYDRAULIC DRAWING --------------------------------------- 26
APPENDIX

The sterilizer described in this manual is intended for the sterilization
of research tools. It operates automatically with 134°C and 121°C
sterilization temperatures. This sterilizer is in compliance with the
European Directive 93/42/CEE and it has been produced in accordance
with the EN 13060. In addition the chamber has been ASME certified.
1 General
4
3
2
1
1 Distilled water tank
2 LED screen
3 Control panel
4 Main Power switch
5 Drain connector of distilled water tank
6 Door handle
7 Drain connector of used water tank
8 USB port (optional)
9 Printer port
10 Printer power
11 Bacteriological filter
12 Safety valve
13 Condenser ventilation
14 Circuit breaker
15 Power supply cord
16 Rating plate
Security Notice
For safe operation, please pay close attention to the alert symbols
below which cab be found throughout this manual. Please
carefully read and understand the contents of this manual prior to
operating this instrument.
This symbol represents an electrical caution - ground protection
HOT SURFACE.
This symbol represents a warning of a potential hot surface.
Important safety information.
This symbol represents a warning for extra caution
5
6
7
11
13
15
12
16
8
9
10
1
14
2 Technical Specifications
Chamber
Rated Voltage
Main Fuses
Sterilization Temperature
Capacity of the distilled
water tank
Operation temperature
Exterior Dimensions
Noise Level
Relative Humidity
Atmospheric pressure
Item
16
Φ230mmX360mm
110V-130V or 220V-240V, AC, 50-60Hz
F25A/250V for 110V
121°C/134°C (250°F/274°F)
Approx 2.5L (water at level MAX)
Approx 0.5L (water at level MIN)
5 - 40℃
445mm(width)X 410mm(height)X 605mm(depth)
76kPa -106kPa
max. 80%, non condensing
<70dB
45kg
2
Nominal power
1900VA
Weight
99 lbs
Instructions manual
Instructions manual

3 Packing Content
Quantity
Item
No.
2
3
4
5
6
7
Instrument tray
Instrument tray rack
Instrument tray handle
Door adjustment tool
Draining hose
Instructions manual
2
1
1
1
2
1
1
1Steam sterilizer
1
3
Door seal
8
4 Installation
4
Instructions manual
Instructions manual
605
Ensure that the sterilizer is installed with 2.5in. (10cm)
ventilation space on all sides of the sterilizer, and 5 in. (20cm)
on top side. The clearance required to open the door is 15.5in.
(40cm).
The sterilizer should be placed on a level worktable.
Do not cover or block the door, ventilation or radiation
openings on the sterilizer.
Do not install the sterilizer near a sink or in a location where it
is likely tobe splashed.
Do not install the sterilizer nearby a heat source.

Distilled water tank is requires water.
Distilled water tank is full
Used water tank is full.
Door locked
information output to USB port
Printer is connected
Notice: Before using the sterilizer or at any time the low water
icon blinks, it is necessary to fill the distilled water
tank with distilled water.
5.1 Setup
5.1.1 Open the door and remove all of the inner contents for unpacking.
5.1.2 Connect the power cord to an outlet of the appropriate voltage
5.1.3 Power on
The switch is located underneath the control panel
on the front side of the machine .
After switching on, the machine turns on the LCD
and shows the door position, water level,
working program, date, time and etc.
5 Operation
Program
Alarm code
process curve
Temperature
Pressure
Setting
Time
Date
5
5.1.4 Filling the distilled water
Remove the cover, and fill the tank with distilled water.
When you hear a beep signal, it means the water level exceeds the
max. level. The will be displayed. Please stop filling immediately.
5.2 Preparation of sterilization materials
For the most effective sterilization and to preserve the sample, please
follow below:
* Arrange the samples of different material on different trays or with at least
2in. of space between them.
* Always insert a sterilization paper or cloth between the tray and sample,
to avoid direct contact between the different materials.
6
The water level should not
exceeded this port.
Instructions manual
Instructions manual
F
F
F

5.3 Selecting the sterilization program
5.3.1 LCD
The panel displays the cycle
temperature, pressure, error code,
sterilization state and program.
5.3.2 Temperature button
Press this button to toggle
between 121°C and 134°C
5.3.3 Program button
Press this button to toggle between
available sterilization cycles (see below)
5.3.4 START/STOP button
Press this button to start the sterilization cycle. To stop a cycle, press
and hold this button for 3 seconds.
Notice: Button will be “locked” for the initial 10 seconds after power
up for system initialization.
78
5.4 Running the sterilization program.
After selecting program, the materials
to be sterilized can now be placed on the
tray and the tray placed inside the
chamber using the tray handle
5.4.1 After the instruments are loaded, you
may close and lock the door by turning
the door handle clockwise until it stops.
The icon will be lightened.
Caution You must turn the door handle to the maximum position,：
otherwise the machine will alarm and prevent
completing the cycle.
Instructions manual
Instructions manual
UNWRAPPED
WRAPPED
EXTENSIVE
LIQUID
DRYING
274 F
F

Caution: When you press the START button but the door has not been
fully closed. You will see the blinking on the screen.
A cycle can not be started until you close the door to the max.
position and press the "Start" button again.
9
5.4.2 Start the sterilization program.
Press START button, the machine will begin the cycle automatically.
It will take 30-75 minutes. (See Appendix 2)
total time or
count down until
completion
After a cycle is completed, the printer will be activated and print out a
report of the cycle settings (if the optional printer has been connected).
After the pressure returns to 0, the door is unlocked and the materials
can be removed.
5.4.3 Sterilization cycle completion
Always use the tray handle to load or unload the tray into the
autoclave. Failure to do so can result in burning.
10
If you need to interrupt a cycle and remove materials urgently.
you may hold the START button for 3 seconds after completing
sterilization time to skip the dry cycle.
This will result in the program skipping directly to the last step
and eliminate the drying stage. After one minute the cycle
will end.
Instructions manual
Instructions manual
F
F

6 Advanced Setting
6.1 Enter the setting
6.1.1 Power on the machine while Holding the
START button and hold for 5seconds. Thiw
will enter into the advanced settings mode.
6.1.2 Select the state (State 1 thru. 3) by pressing
the Program button. Press the START
button to enter the setting.
6.2 S1 state
If you select the S1. You may change
the unit of temperature and pressure,
and adjust time and date.
6.2.1 The first option is to select the unit of
temperature. Press temperature button
to select or℃.
The unit you selected will be lighted. Press
the program button to the next item.
6.2.2 You may select the unit of pressure in the same manner.
6.2.3 Then press program button to the next item to adjust the time and
date. After the last word of the date or time is set , then the data is
permitted to be saved. If you want to finish the setting you shall press
START. It will return to the screen of selecting states.
The Machine No. And cycle No can not be set by the operator.
6.3 S2 state
6.3.1 You may check the count of sterilization
cycle. It can not be changed by operator.
6.3.2 Set the parameter for high altitude.
If you have trouble completing a cycle in a
location of high altitude (above 2.0 km or
atmospheric pressure is below 80kPa)
you may need to adjust this parameter.
6.3.3 Language set:
00 English 01 German 02 Spanish
03 Polish 04 French 05 Magyar
06 Romanian 07 Dutch 08 Lithuanian 09 Latvian
cycle No
machine No
Altitude set
Language
set
1211 Instructions manual
Instructions manual
F
6.5 Printer (Optional)
6.5.1 Connect the printer cable.
6.5.2 Connect the printer power.
holding time
drying time
Notice: the default sterilization parameters have been chosen to
provide optimal sterilization results. We do not suggest adjusting
these parameters unless it is necessary.
6.4 S3 state
6.4.1 Adjust the length of sterilization and drying time.
Press program button to select the program.( )
Press temperature button to select the temperature of program.
Then press START to adjust the drying time and holding time.
6.4.2 First to adjust the holding time.
Press temperature button to adjust the data.
Press the program button to select
the items.
6.4.3 Press START to save .
6.4.4 Drying time is 0-19.
Holding time of 250 is 1-59.
Holding time of 274 is 1-19.
F
F
274 F

13
6.7 Retrieve information from a prior cycle
Press PROGRAM repeatidly until you enter
the prior program storage screen.
This will show the cycle No.
Press the TEMP button to toggle between
different cycles.
To print or send details to the USB drive,
press the START button. The most recent
20 records are stored.
When vieing printed data records, refer to the diagram below:
14
Instructions manual
Instructions manual
A USB drive can be used as a method of storing a report of the
cycle. To do so, insert the USB drive to the slot on the right side of
the instrument.
The information will automatically output directly to the USB
after the cycle has completed.
The name of the file is determined by the serial number of
the machine and the cycle number.
:For example
The serial number is E00001 The cycle number is 00012..
10 . .The file name in the USB stick is 0 0 1200 txt
.The first two numbers represent machine number
.The middle four numbers represent cycle number
.The last two numbers represent error code
:;:00 no error 01 error E01
6.6 USB Flash memory (Optional)
12
t1T3t5
t8
t2t4
t7
ts
P
t
t6
=========================
Program: WRAPPED
Temperature: 274 F
Pressure:30.5 psi
Dry Time: 03Min
Ster Time: 4.0Min
-------------------------------------
time temp. pressure
Start 15:24:20 107.6 F
T1: 15:32:11 158.0 F 07.71psi
T2: 15:36:08 167.5 F 01.42psi
T3: 15:39:21 194.5 F 07.30psi
T4: 15:44:32 094.3 F 01.39psi
T5: 15:47:12 201.7 F 14.91psi
T6: 16:00:11 230.3 F 01.35psi
TS: 274.6 F 32.14psi
Max.Temperature:275.2 F
Min.Temperature:274.1 F
Max.Pressure:33.42psi
Min.Pressure:30.88psi
T7: 16:04:02 275.0 32.42psiF
T8: 16:06:32 274.6 F 31.05psi
End 16:14:12 172.8 F
--------------------------------------
Cycle No: 0005
Ster Value: Success
Date: 2011-01-18
SN:E00001
Operator:
=======================
=========================
Program: WRAPPED
Temperature: 274 F
Pressure:30.5 psi
Dry Time: 03Min
Ster Time: 4.0Min
-------------------------------------
time temp. pressure
Start 17:34:20 179.6 F
T1: 17:42:11 194.0 F 07.57psi
T2: 17:46:08 185.5 F 01.41psi
T3: 17:49:21 226.9 F 07.75psi
T4: 17:54:32 212.5 F 01.39psi
T5: 00:00:00 000.0 F 000.0psi
T6: 00:00:00 000.0 F 000.0psi
TS: 000.0 F 000.0psi
MAX.Temperature:000.0 F
MIN.Temperature:000.0 F
MAX.Pressure:000.0psi
MIN.Pressure:000.0psi
T7: 00:00:00 000.0 F 000.0psi
T8: 00:00:00 000.0 F 000.0psi
End 17:54:42 212.4 F 01.46psi
--------------------------------------
Cycle No: 0007
Ster Value: Failure E01
Date: 2011-01-18
SN:E00001
Operator:
=======================

15 16
Instructions manual
Instructions manual
Clean the external surface
Maintenance Operation
Clean the door seal
Clean the distilled water tank
Clean the sterilization chamber
Replace the door seal
7.1 Clean the distilled water tank every
week with isopropyl alcohol or a
medical disinfectant.
7.2 Clean the chamber weekly.
7.2.1 Remove all trays and the tray rack
from the chamber.
7.2.2 Clean the chamber with a
smooth cloth saturated with
distilled water.
7.2.3 Apply the same procedure for
the trays and rack.
Frequency
7. Maintenance
7 Maintenance
Weekly
Every year
Daily
7.4 Clean the door seal
Clean the door seal weekly, with a smooth cloth saturated with the
distilled water.
Fig 1 Fig 2
Caution: Never adjust the chamber door while the door is closed.
7.5 Door adjustment
On normal circumstance the chamber door does not require adjustments.
However, if the seal fails (resulting in steam leaking from the front of
the chamber), you may use the included tool to tighten the door seal.
7.5.1 Open the door
7.5.2 Insert the spanner tool in the gap beneath the plastic cover; use the spanner
to grip the adjusting nut (Fig 1). Turn the nut counter clockwise as
the figure below (Fig 2). This will tighten the sealing plate.
7.5.3 Turn the nut until the sealing plate is tight. If the door knob is too tight,
you may also turn the nut clockwise to loosen it.

17
7.6 Replacement of the door seal
7.6.1 Open the chamber door.
7.6.2 Remove the door seal ring carefully by hand.
7.6.3 Clean the door seal ring carefully with a smooth cloth
saturated with distilled water.
7.6.4 Moisten the new seal with medical disinfectant or
isopropyl alcohol.
7.6.5 Insert the new seal and press in sequence as follows:
Caution: Please ensure the chamber and the door have cooled
prior to replacing the seal ring.
1). Press in the top and bottom
of the seal ring.
2). Press in the left and right sides
of the seal ring.
3). Press the remaining sections
of the seal ring.
4). Press all areas of the seal
surface for a smooth finish.
18
8 Transportation and Storage
8.1 Switch off the sterilizer before transportation or storage. Pull out the
plug to let the machine cool down.
8.2 Drain the distilled water tank and the used water tank
8.3 Conditions for transportation and storage:
Temperature: -20 ~ +55
Relative humidity: 85%≥
Atmospheric pressure: 50kPa~106kPa
7.7 The drain valve
1.Press the included hose on to the
drain valve firmly.
2.Set the drain valve to the open
position by turning it counter
clockwise.
3.Pull the drain valve outward, the
tank will begin to drain.
4.After finish draining the tank, push
the drain valve inward and turn
clockwise into the closed position .
Instructions manual
Instructions manual

19
9 Error codes
Code
E1
E2
E3
E4
E5
E6
Description Proposed solution
Steam generator temperature
sensor error
Temperature sensor of chamber
wall error
Inner temperature sensor error
Fail to rise temperature
Fail to release the pressure
Door has opened during the
cycle
Failure to hold temperature
Program manually interrupted
Power off & run a new cycle
Contact your Supplier if error persists
Power off & run a new cycle
Contact your Supplier if error persists
Carefully ensure that the chamber wall
is heated. If not contact your supplier
Check to ensure that the used water
valve is fully closed.
Power off & run a new cycle
Contact your Supplier if error persists
Make sure you have turned the door handle
to the max. Position or check the door switch
Shut off the power and restart the
power
Ensure the distilled water tank isn't empty
Check the inner temperature sensor
Check the door for leaking
E20
E9
Failure to preheat the chamber
E12
E11 Failure to preheat the steam
generator
Check the steam generator heater
Check the steam generator protector
Check the chamber heater
Check chamber protector
20
(1)Main fuses
Protect the instrument against possible failures of the
heating resistor .
Action: Interruption of the electric power supply.
(2)Thermal cutouts on the main transformer windings
Protection against possible short circuit and main transformer primary
winding overheating .
Action: Temporary interruption of the winding.
(3)Safety valve
Protection against possible sterilization chamber over-pressure .
Action: release of the steam and restoration of the safely pressure.
(4)Safety micro-switch for the door status
Comparison for the correct closing position of the door .
Action: signal of wrong position of the door.
(5)Door safety lock
Protection against accidental opening of the door.
Action: Impediment of the accidental opening of the door during the program.
(6)Self-leveling hydraulic system
Hydraulic system for the natural pressure levelling in case of manual cycle
interruption, Alarm or black-out .
Action: automatic restoration of the atmospheric pressure inside chamber.
10 Safety devices
Instructions manual
Instructions manual

21
APPENDIX 1
Water Properties/Characteristics
DESCRIPTION FEED WATER CONDENSATE
Evaporate residue
Silicium oxide sio2
Iron
Cadmium
Lead
Rest of heavy metals, excluding
iron, cadmium, lead
Chloride
≥10 mg/l
≥1 mg/l
≥0.2 mg/l
≥0.005 mg/l
≥0.05 mg/l
≥0.1 mg/l
≥2 mg/l
≥1.0 mg/kg
≥0.1 mg/kg
≥0.1 mg/kg
≥0.05 mg/kg
≥0.1 mg/kg
≥0.1 mg/kg
≥0.1 mg/l
Phosphates
Conductivity (at 20 )℃
pH value
Appearance
Hardness ≥0.02 mmol/l
Colorless, clean,
without sediments
575-.
≥μ15 s/cm
≥0.5 mg/l ≥0.1 mg/l
≥μ3 s/cm
57-
≥0.02 mmol/l
Colorless, clean,
without sediments
22
DIAGRAMS OF THE STERILIZATION PROGRAMS
APPENDIX 2
Instructions manual
Instructions manual
The max. temperature of the 274 F sterilization cycle is 279 F
The max. temperature of the 0 F sterilization cycle is 256 F
PROGRAM
UNWRAPPED
LIQUID
EXTENSIVE
DRYING
Temperature
()F
274 30.5 4 14 30-
274 30 5 18 35 55.-
274 30.5 10 30 55-
250 30 35 60- 16.0
250 16.0 20 30 45-
。
Pressure
()psi
Holding time
()min
Total time
()min
120-
Unwrapped porous material
Single wrapped porous-
material
Single wrapped hollow-
material
Dual wrapped porous-
material
Dual wrapped solid and-
hollow material
Unwrapped solid
material
TYPE
Liquid
300 100..
200 075..
100 030..
075 020..
050 015..
Max Load.
400 120..
120 040..
()kg
Max Load.
per tray kg()
274 30.5 4 30 50-
250 20 35 55- 16.0
WRAPPED
Unwrapped porous material
Single wrapped solid or-
hollow material
4 00 1 20..
3.00 1 00.

23
-.08 -.08
-.08 -.08
00.00.
00.00.
10.10.
10.10.
20.20.
20.20.
UNWRAPPED
Pressure(bar) Pressure(bar)
Pressure(bar) Pressure(bar)
Time(min) Time(min)
Time(min) Time(min)
24
WRAPPED
DRYING
EXTENSIVE
DRYING
LIQUID
Instructions manual
Instructions manual

25
WIRING DIAGRAM
TANK MAX. LEVEL
PRESSURE SENSOR
V4
V1
PRINTER
POWER
KEYBOARD
DATA LINE
DOOR CLOSE
Tp1
Tp2
Tp3
～230V(120V)
VACUUM
PUMP
～9V(2.5A)
～9V(0.2A)
～21.5V
～0V
FAN
WATER
PUMP
STEAM
HEATER
THERMAL
PROTECTOR
CHAMBER
HEATER
THERMAL
PROTECTOR
PUBLIC
TANK MIN. LEVEL
PRINTER
OUTPUT
USB DATA
OUTPUT
USED WATER TANK
26
TP1: Steam generator temperature sensor
TP2: Inner temperature sensor
TP3: Temperature sensor of chamber wall
V1: Air release valve
V4: Water release valve
230V (120)
-
+
Instructions manual
Instructions manual
APPENDIX 4
HYDRAULIC DRAWING
V1: Air release valve
V4: Water release valve
Chamber
Pressure
sensor
Safety valve
Pump
Condenser
Used water tank
Distilled water tank
APPENDIX 3