Benchmark Scientific Accuris User manual

Automatic Cell Counter
Instruction manual
Accuris Instruments
A division of Benchmark Scientific
PO Box 709, Edison, NJ 08818
Tel: [phone]
E-mail: [e-mail]
Website www.accuris-usa.com
Copyright © 2020, Benchmark Scientific.
All rights reserved.

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Package contents
QuadCount™ Automatic cell counter package includes the following items.
Item
Quantity
QuadCount™ main device
1
USB Memory Stick
1
Quick manual (PDF on Memory Stick)
1
Instruction manual (PDF on Memory Stick)
1
Main Power Cable
1
QuadCount™ Slides (Optional)
50 ea. per box
Keypad (Optional)
1
Barcode scanner (Optional)
1
Thermal printer (Optional)
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When receiving the package,
• Check that all items listed above are included in your package.
• Examine the device carefully for any damage during shipping.
• Contact your local distributor or [email protected] if any items are missing or damaged.
• Any loss or damage claims must be filed with the carrier.

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Safety instruction
READ ALL INSTRUCTION BEFORE USE
Caution
• Check the power supply input voltage and make sure it matches the wall outlet voltage.
• Check that the power cable is connected to a grounded, 3-pin wall outlet.
• Check that the power cable is properly grounded to avoid potential electrical shock.
• Check that the main power switch is off when plugging in the power cable to the wall outlet or
when unplugging the power cable.
• Power on using the main switch on the rear panel, wait about 2-3 minutes for the device to reboot.
• Do not insert any metallic objects into the device through backside air vent to avoid electrical shock
causing personal injury or device damage.
• Place the device in an area where there is 10 cm clearance from other objects to allow for proper
air-cooling.
• Do not disassemble the device. If service is needed, contact Accuris Instruments or an authorized
distributor.
• Use authorized accessories only.
• Operator should have the general knowledge of laboratory techniques and cell counting
procedures as well as safe handling of biological samples.
• Operate the device carefully as described in this manual.
Warning
• Battery
There is a Lithium battery is inside the device. Replacing it with an incorrect type can cause risk of
explosion. This battery should not be replaced by the user; contact Accuris an authorized service
center if required.
• Sample handling
Samples may contain infectious biohazardous substances. Operator should wear gloves while
handling all samples.
• Waste
Dispose used QuadCount™ Slides as biohazardous waste and do not reuse them.

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Product specifications
QuadCountTM
Voltage
AC 100~240 V, 50~60 Hz
Current
Max. 1.0 A, 50 W
Objective lens
4 x
Light source
4 W Green LED
Camera
5Mega pixels high resolution
monochrome CMOS image
sensor
Weight
5 Kg
Size (W × L × H)
163 × 293 × 216 mm
Measuring
concentration range
1 x 104 ~ 1 x 107 cells/mL
Detectable cell
diameter
5 ~ 60µm
Measuring speed*
Quick mode: ≈ 20s per test
Normal mode: ≈ 30s per test
Precise mode: ≈ 100s per test
Counting area
Quick mode: ≈ 0.15 µL
Normal mode: ≈ 0.9 µL
Precise mode: ≈ 3.6 µL
QuadSlides™
(Cat. No.
E7500-S1
(Order
separately)
Quantity
50 slides per box (for 200 tests)
Sample loading
volume
20 µL
Accessories
Power cable
1.5 m
USB memory stick
Supports USB 2.0
(Optional)
Keypad, Barcode
scanner, thermal
printer
USB type
*Cell Counting time can vary depending on cell type and concentration.

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Device overview
Front view
• Slide holder door – Slide holder is ejected from / inserted into the device.
• Touch LCD display – Preview, automatic cell counting processes and the results are displayed.
• 3 control buttons
Slide holder door
Touch screen LCD display
Button 3(Setting screen)
Button 1(Insert/Eject slide holder)
Button 2(Home screen)

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Rear view
• 3 USB ports – Keypad, Barcode scanner, Thermal printer (optional), or USB memory are
connected to these ports.
• Ethernet port – LAN cable is connected to this port for PC interface.
• Power switch – Main device power ON/OFF control.
• Power cable socket – Power cable is connected to this socket.
USB Port
- Keypad
- Barcode Scanner
- Thermal Printer
- USB Memory
Ethernet Port
- PC Interface
Power Switch
Power Cable Socket

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Table of contents
Package contents 3
Safety instruction 4
Product specifications 5
Device overview 6
Introduction
QuadCount™– Automatic cell counter 10
QuadSlides™ (50 slides for 200 tests per a box, Cat. No. E5750-S1)
Getting started
Pre-requirements 12
Basic installation 13
Power up and initial display 14
General operation
Sample preparation 15
Basic operation 16
Preview prior to counting 18
Stopping while counting
Set counting option 21
A. Changing user group 22
B. Setting Count mode 23
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C. Creating a Preset 24
D. Editing a Preset 27
E. Selecting Channels
F. Entering a Channel ID 30
The result screen
A. Analyzing by Histogram 36
B. View results images
C. Printout Cell Count Result using Thermal Printer 40
D. Exporting the Report to a USB memory stick
E. Exporting Data (all history) to a USB memory stick 43
F. Showing channel ID names
Setting screen
A. Checking Firmware information and Updating Firmware 48
B. Bead Quality Control 50
C. Setting Date and Time
Maintenance and cleaning
Appendix
A. Trouble shooting 54
B. Examples of errors and inaccurate results
C. The contents of result data exported as a .csv file
D. Example and explanation of PDF report 58
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Introduction
QuadCount™– Automatic Cell Counter
The QuadCount™ is a fully automated cell counting system based on a brightfield microscopy
technique for mammalian cell counting. The QuadCount™ utilizes a high-powered LED light source,
CMOS image detection (5 Mega pixels), precise X-Y-Z stages and on-slide image processing
technologies for fast and accurate cell analysis.
Cell counting using the QuadCount™ requires 3 main steps, (1) cell staining, (2) loading
sample slide, and (3) counting. Cells are mixed with trypan blue dye to distinguish between live and
dead cells. The stained sample is pipetted into the disposable plastic slide (4 tests per slide) and the
slide is loaded into the QuadCount Instrument. After loading the slide, the optic system
automatically focuses on the slide and the instrument acquires and analyzes the images
automatically. The precise X-Y-Z stages move through the preset routes to take multiple images for
each channel. A highly sensitive CMOS sensor acquires bright-field microscopy images and sends
them to the integrated system for image processing and analysis. The whole counting process takes
2 minutes (in Normal mode) and the counting results are displayed on the LCD touch screen panel
on front of the instrument.

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QuadSlides™ Slides (50 slides for 200 tests per a box, Cat. No. E7500-S1)
The QuadSlide™ is a disposable plastic hemocytometer that includes 4 sample channels engraved
with Neubauer Improved pattern. Each channel has an enclosed structure of 100um depth and a
hydrophilic surface. The precise capacity and diffusible surface ensures that cells are
evenly distributed and this ensures accurate analysis. QuadSlides™ can be used for mammalian
cell counting with the QuadCount Instrument, but can also be used for manual counting methods.
The measuring range of cell concentrations is 1 x 104 ~ 1 x 107 per mL when used with the QuadCount
Instrument.
Cell Counting: prepare a cell suspension for counting and mix the cell suspension with trypan
blue at a one to one ratio. Each channel of a QuadSlide™ is filled with 20 μL of mixture and is then
loaded into the QuadCount™ Instrument. After the analysis is complete, the results will be displayed.
Keep QuadSlide™ boxes upright and at room temperature. Each individual slide should be
used immediately after opening the individual sealed package. Follow the exact procedure detailed
in the Instructions for Use section.

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Getting started
Pre-requirements
For normal and stable operation of the device, the following environmental conditions should be
met.
• Room temperature between 20 ~ 35 °C (68 to 95 °F)
It is not recommended to operate the device at low temperature condition (below 10 °C)
In cold conditions, warm up the device for a minimum of 10 minutes before use.
• Relative humidity between 0 ~ 95 %.
• Install in a location free of corrosive gases or other corrosive substances.
• Install in an area free of dust or other airborne particles.
• Avoid direct sunlight, vibration, and close proximity to magnetic or electromagnetic fields.
• Do not place any heavy objects on the top of the device.

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Basic installation
1. Unbox the QuadCount™ and place the
device on a flat, level and dry surface.
2. Plug accompanying power cable into the
power cable socket.
3. Connect any optional peripherals (keypad,
barcode scanner, or thermal printer) to the
USB port if desired.
4. Plug the power cable into an appropriate
rated wall outlet and press the power switch
to ON.
Check that the main power switch is in I (ON)
position.

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Power up and initial display
1. Once the main power is switched on, the
boot image is displayed on the LCD touch
screen. When booting is completed, the
initializing process starts and internal
motorized stages start moving.
2. Initializing progress is displayed while
processing.
3. When initializing is finished, the slide holder
is ejected, and Home screen is displayed on the
LCD touch screen.
4. After loading a slide with sample, the device
is ready to count.

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General Operation
Sample Preparation
Required materials: Cell suspension, 0.4% trypan blue, micro tube 1.5ml, pipette, tips, and
QuadSlides™. Preparation should be done in a clean area to avoid dust contamination (dust on the
slides or in the samples will greatly reduce counting accuracy).
STEP 1. Prepare necessary items.
STEP 2. Place 20 μL of trypan blue in the micro tube and add an equal volume of the cell
suspension.
NOTE: Before sampling the cell suspension, gently resuspend the cells at least 6 times
(pay attention to avoid bubbles and check if there are any cell clumps or agglomerates)
The sampling should be in middle of the cell suspension, not on the surface or the bottom.
STEP 3. Mix the sample in the micro tube by pipetting the vial 3~5 times gently.
NOTE: Be careful not to create bubbles.
STEP 4. Load 20 μL of the stained cell sample into each channel of a QuadSlide™.
NOTE: The samples should be from the middle of the cell suspension, not from the surface or the
bottom, and ensure that no bubbles enter the slide channesl.

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Basic Operation
STEP 1. Insert a QuadSlide™ loaded with the samples into the slide holder.
NOTE: Make sure the arrow on the slide points toward the instrument.
STEP 2. Press Start button to start counting procedures. The slide holder will retract
automatically, and auto-focusing is performed prior to counting each sample.

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STEP 3. The counting progress is indicated as shown in following image. For completion of each
sample, the count results (unit: x104/mL) are displayed.
STEP 4. Once counting is complete, the slide holder is ejected automatically. Remove the QuadSlide™
from the slide holder.
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1
Home
260 40 15
340 140 41
500 420 84
200 100 50

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Preview prior to counting
On the screen where you can see the cells, tap the screen twice to make the icons disappear.
To get icons back again, tap the screen twice.
STEP 1. Load a slide and press Review button.
STEP 2. Select a channel to preview.
STEP 3. Positioning and Autofocusing happens automatically

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STEP 4. See the cell image of the selected channel.
STEP 5. Press Mark , and the detection mark is displayed. Live/Dead definition can be modified
at this stage.
STEP 6. Counting
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Stopping While Counting
STEP 1. To stop the instrument during counting, Press the STOP button.
STEP 2. A confirmation message box is displayed as shown the following image.
Press the Continue button to confirm stopping.
STEP 3. Once stop counting is confirmed, all remaining processes are stopped, and the slide holder
is ejected automatically.
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