Benchmark Scientific BeadBlaster 24 User manual

Instruction Manual
BeadBlaster™ 24
D2400
Homogenizer

Foreword
Thank you for purchasing a BeadBlaster™24 Homogenizer. This manual
contains instructions for the proper operation and care of this instrument.
Please read it carefully before operation and keep it available for future use.
Prior to initial operation:
Please check the instrument and the accessories against the packing list
when you first open the shipping carton. Report any damage, discrepancy,
or missing items to the supplier immediately.

Safety Warnings and Guidelines
1. Important operation information:
Read this manual carefully before attempting to operate the instrument.
2. Safety:
3. The operation, maintenance and repair of the instrument should comply with the
basic guidelines and safety warnings below. Noncompliance may interfere with
the useable life of the instrument and safety protection and may void the warranty.
Use this product indoors. Do not operate in a damp or wet
environment.
Before using the instrument, read the manual carefully. This instrument
is designed for use in laboratory environments and must be operated by
skilled personnel with the appropriate training.
Do not attempt to open the casing or repair the instrument. Disassembly
will void the warranty. If there is a service issue with the instrument,
please contact the supplier.
Read this manual prior to powering up the instrument. Pay special
attention to the guidelines and directions below and be familiar with
all safety warnings and guidelines.
Before operation, check to see that the voltage rating on the instrument
matches your local voltage. The rated electrical load of the outlet
should not be lower than that of the instrument's demand. If the power
cord is damaged, it should be replaced. Do not place the power cord
where it will have objects placed on it or where it will be walked on.
When unplugging the instrument, do not pull the plug out of the socket
by the cord.

4. Maintenance
The tube holder should be cleaned with a cloth dampened with a small amount of
alcohol. The outside of the unit can be cleaned with a damp cloth or mild
detergent if required.
The power switch is located on the rear of the instrument. After use,
the power to the instrument should be turned off. If the instrument will
not be used for a long period of time, it should also be unplugged and
covered with a cloth or plastic to keep it free of dust.
The instrument should be unplugged immediately and the supplier
contacted in the event of:
Liquid leaking into the instrument
Smoke or fire
Abnormal operation - ie sound or smell
The instrument has been dropped or outer housing damaged
Instrument function changes
Install the instrument in a cool, dry location free from dust, direct
sunlight or strong lights. The instrument should be placed away from
corrosive gasses, strong magnetic fields, and heat sources. Allow at
least 4 inches on all sides of the instrument for proper air circulation.
Be sure that the air vent at the back of the instrument is not blocked. If
using two instruments, they should be placed at least 3 feet apart.
If samples are processed for an extended time period, many
cycles or without a pause between cycles, thermal breakdown of
the sample tube may occur. Be sure to follow manufacturers
guidelines for lysing kits and tubes.
Always disconnect power to the unit before any cleaning procedure.
Do not apply cleaning solutions directly to the tube holder or chamber.
Do not use corrosive agents while cleaning. Do not submerge the
instrument.

Contents
CHAPTER 1 INTRODUCTION ------------------------------------------------------------------------------ 1
CHAPTER 2 SPECIFICATIONS ---------------------------------------------------------------------------- 2
1. NORMAL OPERATION CONDITIONS: --------------------------------------------------------------- 2
2. THE BASIC PARAMETERS AND CHARACTERISTICS----------------------------------------- 2
CHAPTER 3 BASIC INSTRUCTIONS--------------------------------------------------------------------- 3
1. STRUCTURE OVERVIEW-------------------------------------------------------------------------------- 3
2. OPERATION PANEL--------------------------------------------------------------------------------------- 4
3. DISPLAY------------------------------------------------------------------------------------------------------- 4
CHAPTER 4 OPERATION GUIDE------------------------------------------------------------------------ 5
1. LOADING TUBES------------------------------------------------------------------------------------------- 5
2. PROGRAM SELECTION---------------------------------------------------------------------------------- 7
3. PROGRAM SETTING-------------------------------------------------------------------------------------- 7
4. RUNNING AND STOPPING PROGRAMMED OPERATION------------------------------------ 8
5. MOTOR OVER TEMPERATURE PROTECTION--------------------------------------------------- 8
6. BEAD SELECTION----------------------------------------------------------------------------------------- 9
7. SAMPLE PREPARATION--------------------------------------------------------------------------------10
CHAPTER 5 TROUBLESHOOTING GUIDE ------------------------------------------------------------V

D2400 Homogenizer Manual Chapter 1 Introduction
─1─
Chapter 1 Introduction
The BeadBlaster™24 is a homogenizer for biological samples. It features high speed,
three-dimensional vibration and has a capacity for 24 samples.
Features:
Can break and crack most biological samples (animal and plant tissue,
soils, yeast, spores, microorganisms, etc.)
Processes 24 samples in a minimal amount of time.
On board memory for 50 programmed runs.
Run stops automatically if the cover is opened.

D2400 Homogenizer Manual Chapter 2 Specifications
─2─
Chapter 2 Specifications
1. Normal operating conditions:
Ambient temperature：5C 35C
The relative humidity：≤70%
Power：AC100-240V～50-60Hz
2. The basic parameters and characteristics
Type
Parameter
D2400
Display
OLED
Speed
4.00m/s~7.00m/s, in 0.05m/s increments
Cycle duration
1s~ 1min 30s, in 1s increments
Pause
1s~ 2min, in 1s increments
Number of Cycles
10
Programs
50
Sample Size
24 X 2.0ml tubes
Acceleration time
＜2s
Deceleration time
＜2s
Noise
＜68db
Power
600W
Dimension(WXDXH)
280mm × 360mm × 385mm
Weight（kg）
25.0kg

D2400 Homogenizer Manual Chapter 3 Basic instructions
─3─
Chapter 3 Basic instructions
This chapter focuses on the structure, operation keys and display of the instrument, as well as
preparatory work before starting. Please read this chapter before initial operation.
1. Structure overview
Cover Latch
On/Off button
Power supply plug
Operation panel
Tube retainer
Display screen
Protective cover
Air opening

D2400 Homogenizer Manual Chapter 3 Basic instructions
─4─
2. Operation panel
3. Display
Prog. Use in conjunction with  to select the desired program.
Set Set speed, run time, cycle and pause time for the current program.
 Modify parameters
Start Run program
Stop Stop program
PROG：01 28ºC
6.50 03
0:30 0:30
SPEED
M/S
M:S
TIME
CYCLE
INTER..
M:S

D2400 Homogenizer Manual Chapter 4 Operation Guide
─5─
Chapter 4 Operation Guide
1. Loading Tubes
1.1 Disengage the locking latch (as shown below), turn the knob counter-clockwise and remove
the tube retainer.
1.2 The tube holder may be filled in or out of the instrument. To remove, simply lift off the shaft.
Place sample tubes into the tube holder and press down until seated. If less than 24
samples are used, they should be evenly distributed in the tube holder. If removed, the tube
holder can then be replaced, making sure the two bumps on the holder line up with the
indentations on the shaft.
Knob
Tube retainer
Locking latch
Sample tube
Tube holder

D2400 Homogenizer Manual Chapter 4 Operation Guide
─6─
1.3 Place tube retainer on top of the tube holder. Tighten the knob clockwise and push the
locking latch inwards. Aclick will indicate that the latch is in place. If the latch cannot be pushed
inwards, turn the knob 5 degrees and try again.
Note：The locking latch must be pushed in the “LOCK” position.
1.4 Close the cover and engage the cover latch. Operation can then begin.
DO NOT ATTEMPT TO OPERATE THE UNIT WITHOUT THE TUBE RETAINER IN PLACE!
Tighten the knob clockwise, and
push the locking latch inwards to
lock the tube retainer in place

D2400 Homogenizer Manual Chapter 4 Operation Guide
─7─
2. Program selection
50 onboard programs are available. To access the programs, press and hold the “Prog.” key, and
then use “”or “”keys to scroll through the programs. To change m/s to rpm, press “”and
“”key simultaneously.
3. Program setting
Step 1: Press “Set” key once, SPEED in display flashes, then press “” or “” to adjust the
speed.
Step 2: press “Set” key again, CYCLE in display flashes, then press “” or “” to set the number
of cycles.
Step 3: press “Set” key again, TIME in display flashes, then press “” or “” to set the run time.
Step 4: press “Set” key again, INTER in display flashes，then press “” or “” to set the pause
time.
Values will be automatically be saved after 3 seconds.
PROG：01 28ºC
6.50 03
0:30 0:30
SPEED
M/S
M:S
TIME
CYCLE
INTER..
M:S

D2400 Homogenizer Manual Chapter 4 Operation Guide
─8─
4. Running and stopping programmed operation
Press “Start” to begin operation.
Press “Stop” to end operation.
Note：Before beginning operation, be sure that the tube retainer is in the proper
position and locked in place.
If there is abnormal noise during operation, press “Stop” at once to stop running, or
turn off using the On/Off switch on the back of the instrument directly.
5. Motor over-temperature protection
An over temperature sensor protects the motor against damage. The display shows the
temperature of the motor. If the temperature goes above 65º, the unit will shut down until the
temperature has dropped below 60ºC. The display of the motor temperature can be turned on or
off. Press the "Prog" and "Set" keys simultaneously to display the following screen:
Press “” or “”, to toggle between ON and OFF. Press start to confirm the selection. If set to
"ON" the temperature of the motor will appear in the screen.
Sensor: on
Press run to return
Stop
On/Off button

D2400 Homogenizer Manual Chapter 4 Operation Guide
─9─
6. Bead selection
Triple-Pure Zirconium
Stainless Steel
0.1
0.5
1
1.5
3
2.8
Tissue:
Skin
•
Hair
•
•
Nail
•
•
Bone
•
•
Teeth
•
•
Lung
•
•
Muscle
•
•
Liver
•
•
Brain
•
Heart
•
•
Kidney
•
Pancreas
•
•
Fly
•
•
Tick
•
•
Gonad
•
•
Artery
•
•
Intestine
•
•
Cornea
•
•
Tail
•
•
Spleen
•
•
Ear
•
•
Microbial:
Gram +/Gram -
•
•
Yeast
•
•
Fungi
•
•
Bacteria
•
•
Spores
•
•
Mold
•
•
Plant:
Soft Plant Tissue
•
•
Tough Plant Tissue
•
•
Leaves/Stems
•
•
Arabidopsis
•
•
Seeds/Corn/Nuts
•
•
Rice
•
•
Wheat
•
•
Fecal:
Stool:
•
•
Scat
•
•
Soil:
Soil
•
•
Coral
•
•

D2400 Homogenizer Manual Chapter 4 Operation Guide
─10 ─
Bead Ordering Information
All beads are sold in prefilled, 2.0ml tubes, packs of 50
Standard Glass Beads, acid washed
D1031-01 0.1mm
D1031-05 0.5mm
D1031-10 1.0mm
Triple pure Zirconium Beads, acid washed, heat treated and tested to be nuclease free
D1032-01 0.1mm
D1032-05 0.5mm
D1032-10 1.0mm
D1032-15 1.5mm
D1032-30 3.0mm
D1032-SK Starter kit containing 10 tubes each of 0.1mm, 0.5mm, 1.0mm, 1.5mm and 3.0mm
Standard Stainless Beads, acid washed
D0133-28 2.8mm
7. Sample preparation
As heat generated by grinding process can cause damage to the sample, it is recommended that
the samples be added to the tube containing the beads and then stored for 12 hours at low
temperature (below -60℃), to achieve the best results

D2400 Homogenizer Manual Chapter 5 Troubleshooting Guide
─11 ─
Chapter 5 Troubleshooting Guide
Problems and actions
No.
Common problem
Possible cause
Action(s)
1
No display on the screen
No power
Check power supply and that
unit is plugged in properly
On/Off switch broken
Change switch
Faulty fuse
Change fuse（5X20 250V 10A）
Others
Contact supplier
2
“Error: lid”displayed,
audible alert
Faulty button -
protection cover
Contact supplier
3
“open”displayed,
audible alert
Motor over temp
protection- open circuit
Contact supplier
4
Instrument does not run
Motor temp above 65º
Wait for temp to drop below 60º
Faulty Controller
Contact supplier
Faulty Motor
5
Abnormal running noise
Tube retainer not locked
in place
Place tube retainer in place and
lock down properly
Faulty bearing
Contact supplier
6
Keys don’t work
Plug connector loose
Contact supplier
Faulty key
Contact supplier

Memo