Leica CM1520 User manual

Instructions for Use
Leica CM1520, V1.2 – 09/2012, English
SAP: RevC - 14 0491 81101
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Leica CM1520
Cryostat

3
Leica CM1520 – Cryostat
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.leica-microsystems.com
contract provisions agreed between ourselves
and our customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – includ-
ing any electronic systems and media – requires
express prior permission in writing by Leica
Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the nameplate on
the back of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for errone-
ous statements, drawings, technical illustrations
etc. contained in this manual. In particular, no
liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information regarding the contents or technical
details of the present Instructions for Use are not
to be considered warranted characteristics of
our products. These are determined only by the
NOTE

4Instructions for Use V 1.2 – 09/2012
Table of Contents
Table of Contents................................................................................................................................................. 5
1. Important Information ............................................................................................................................. 6
1.1 Symbols in the text and their meanings ................................................................................................... 6
1.2 Qualification of personnel........................................................................................................................... 8
1.3 Intended use of instrument........................................................................................................................ 8
1.4 Instrument type............................................................................................................................................. 8
2. Safety ......................................................................................................................................................... 9
2.1 Safety notes .................................................................................................................................................. 9
2.2 Warnings ....................................................................................................................................................... 9
2.3 Safety devices ............................................................................................................................................ 10
2.3.1 Locking/unlocking the handwheel .......................................................................................................... 10
2.3.2 Knife guard .................................................................................................................................................. 10
2.4 Operating conditions ................................................................................................................................. 11
2.5 Operating the instrument .......................................................................................................................... 12
2.6 Cleaning and disinfection ......................................................................................................................... 12
2.7 Removing the microtome .......................................................................................................................... 13
2.8 Maintenance............................................................................................................................................... 13
3. Technical Data ....................................................................................................................................... 14
4. Setting up the Instrument ..................................................................................................................... 16
4.1 Installation site requirements .................................................................................................................. 16
4.2 Unpacking instructions for the CM1520 cryostat.................................................................................. 17
4.3 Standard delivery ....................................................................................................................................... 20
4.4 Assembling the handwheel ...................................................................................................................... 21
5. Instrument Setup.................................................................................................................................... 22
5.1 Electrical connection................................................................................................................................. 22
5.2 Prior to operation ....................................................................................................................................... 22
5.3 Overview of the Leica CM1520................................................................................................................. 24
5.4 Power switch and circuit breaker........................................................................................................... 26
5.5 Switching on the instrument .................................................................................................................... 26
6. Operating the Instrument...................................................................................................................... 27
6.1 Control panel 1............................................................................................................................................ 27
6.2 Programming the desired values............................................................................................................. 27
6.2.1 Setting the time........................................................................................................................................... 27
6.2.2 Setting the automatic defrost time (cryochamber) .............................................................................. 28
6.2.3 Selecting the cryochamber temperature............................................................................................... 28
6.2.4 Manual defrosting of the quick freeze shelf.......................................................................................... 28
6.2.5 Manual defrosting of the cryochamber.................................................................................................. 29
6.2.6 Setting the section thickness................................................................................................................... 29
6.3 Display lock ................................................................................................................................................. 30
6.4 Control panel 2 - Electric coarse feed .................................................................................................... 30

5
Leica CM1520 – Cryostat
7. Daily Use of the Instrument.................................................................................................................. 31
7.1 Specimen freezing ..................................................................................................................................... 31
7.1.1 Quick freeze shelf....................................................................................................................................... 31
7.1.2 Stationary heat extractor (optional) ............................................................................................ 32
7.2 Specimen discs .......................................................................................................................................... 32
7.2.1 Inserting the specimen discs into the specimen head ........................................................................ 32
7.2.2 Specimen orientation ................................................................................................................................ 32
7.3 Sectioning.................................................................................................................................................... 33
7.3.1 Blade holder CE .......................................................................................................................................... 33
7.3.2 Blade holder CE-TC.................................................................................................................................... 39
7.3.3 Knife holder CN........................................................................................................................................... 40
7.3.4 Knife holder CN-Z with anti-roll guide .................................................................................................... 44
7.3.5 Cleaning the blade and knife holders .................................................................................................... 44
7.3.6 Installing the shelf (movable) (optional) ................................................................................................. 46
7.4 Temperature selection chart (in minus °C) ............................................................................................ 47
7.5 Defrosting .................................................................................................................................................... 48
7.5.1 Automatic defrosting of the cryochamber............................................................................................. 48
7.5.2 Manual defrosting of the cryochamber.................................................................................................. 48
7.5.3 Manual defrosting of the quick freeze shelf.......................................................................................... 49
7.6 Terminating work ....................................................................................................................................... 49
7.6.1 Terminating daily work.............................................................................................................................. 49
7.6.2 Shutdown for a longer period .................................................................................................................. 50
8. Troubleshooting ..................................................................................................................................... 51
8.1 Error messages in the display.................................................................................................................. 51
8.2 Temperature control button ..................................................................................................................... 52
8.3 Possible sources of error, causes and remedies ................................................................................. 53
8.3.1 Changing the battery ................................................................................................................................. 57
9. Cleaning, Disinfection, Maintenance ................................................................................................ 58
9.1 Cleaning ....................................................................................................................................................... 58
9.2 Spray disinfection with Leica Cryofect................................................................................................... 59
9.3 Maintenance............................................................................................................................................... 60
9.3.1 General maintenance instructions.......................................................................................................... 60
9.3.2 Replacing the fuses ................................................................................................................................... 62
9.3.3 Replacing the fluorescent lamp............................................................................................................... 63
10. Ordering Information, Components and Consumables................................................................... 64
10.1 Ordering information.................................................................................................................................. 64
10.2 Mobile heat extractor – application............................................................................................ 89
10.3 Cold extractor – application ....................................................................................................... 89
11. Warranty and Service ........................................................................................................................... 90
12. Decontamination Certificate (master) ............................................................................................... 91
Table of Contents

6Instructions for Use V 1.2 – 09/2012
1.1 Symbols in the text and their meanings
Useful tips,
i.e. important information for the user,
appear in gray boxes and are marked
by an .
Numbers in parentheses refer to item
numbers in illustrations or to the illus-
trations themselves.
(5)
(Fig.5)
1. Important Information
Warnings
appear in a gray box and are marked
by a warning triangle .
REF
SN
Manufacturer
This product fulfills the requirements
of the Council's Directive 98/79/EC
concerning in vitro diagnostics (IVD)
medical devices.
In vitro diagnostics (IVD) medical device
Observe the Instructions for Use
Order No.
Serial number
The CSA test mark means that a prod-
uct has been tested and fulfills the
applicable safety and/or performance
standards, including the relevant stan-
dards defined or administered by the
American National Standards Institute
(ANSI), Underwriters Laboratories (UL),
the Canadian Standards Association
(CSA), the National Sanitation Founda-
tion International (NSF) and others.
Symbol for labeling electrical and electron-
ic equipment in accordance with Section 7
of the German Electrical and Electronic
Equipment Act (ElektroG). ElektroG is the
law regarding the sale, return and environ-
mentally sound disposal of electrical and
electronic equipment.
Environmental protection symbol of the
China RoHS directive. The number in
the symbol indicates the "Environment-
friendly Use Period" of the product. The
symbol is used if a substance restricted
in China is used in excess of the maxi-
mum permitted limit.

7
Leica CM1520 – Cryostat
The package contents are fragile and
must be handled with care.
Indicates the correct upright position of
the package.
The package must be kept in a dry en-
vironment.
Indicates the temperature
range permitted for storing
and handling the package.
Minimum +5 °C
Maximum +50 °C
Indicates the temperature
range permitted for transport-
ing the package.
Minimum -40 °C
Maximum +55 °C
It is not permitted to stack packages,
and no loads may be placed on top of
the package.
1. Important Information
Tip-n-Tell indicator to moni-
tor whether the shipment has
been transported and stored
in upright position according
to your requirements. With a
pitch of 60° or more, the blue
quartz sand flows into the ar-
row-shaped indicator window
and sticks there permanently.
Improper handling of the ship-
ment is immediately detectable
and can be proven definitively.
Example of labeling
in accordance with
IPPC
Package labeling in accor-
dance with German Hazard-
ous Freight Ordinance Road
and Rail (GGVSE)/European
Agreement concerning the
International Carriage of
Dangerous Goods by Road
(ADR) for transporting haz-
ardous goods.
Class 3: "FLAMMABLE
LIQUID"
• IPPCsymbol
• Countrycodeinaccordancewith
ISO 3166, e.g. DE for Germany
• Regionalidentifier,e.g.HEforHessen
• Producer/treatmentprovidercode,
unique assigned number starting
with 49
• Treatmentcode,e.g.HT(heattreat-
ment), MB (methyl bromide), and
possibly DB (debarked)

8Instructions for Use V 1.2 – 09/2012
1. Important Information
1.4 Instrument type
All information contained in these Instructions
for Use applies exclusively to the instrument type
indicated on the title page. A nameplate bearing
the instrument serial number (SN) is attached to
the back of the instrument.
These Instructions for Use include important
instructions and information related to the oper-
ating safety and maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
If additional requirements on accident prevention
and environmental protection apply in the country
of operation, these Instructions for Use must be Fig. 1
1.3 Intended use of instrument
The Leica CM1520 is a high-performance cryostat with disinfection system for routine and research
applications in biology, medicine and industry. The instrument is used to freeze and section speci-
men material quickly. The instrument is not designed for unsupervised storage of specimen material.
The instrument may only be operated within the scope of its designated use as described above and
as per the instructions given in these Instructions for Use. The Leica CM1520 is also suitable for IVD
(in vitro diagnostics).
Any other use of the instrument is considered improper.
In no case does chemical disinfection replace the manual protection as per the applicable lab-
oratory regulations (i.e. safety goggles, gloves, laboratory coat and mask must be worn).
1.2 Qualification of personnel
Only trained laboratory personnel may operate the Leica CM1520. Prior to operating the instrument,
the operator must thoroughly read and understand these Instructions for Use and must familiarize him/
herself with all technical details of the instrument.
The nameplate shown here is intended as an
example only!
supplemented by appropriate instructions to en-
sure compliance with such requirements.
Proper and intended use includes the observance
of all instructions in the Instructions for Use and
compliance with all inspection and maintenance
instructions.

9
Leica CM1520 – Cryostat
2. Safety
2.1 Safety notes
These Instructions for Use include important in-
structions and information related to the operating
safety and maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near the
instrument.
This instrument has been built and tested in ac-
cordance with the safety requirements for elec-
trical equipment for measurement, control, and
laboratory use.
Residual risks
The instrument has been designed and con-
structed with the latest state-of-the-art technol-
ogy and according to recognized standards and
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Operating the instrument safely is, above all, the responsibility of the owner,
as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
regulations with regard to safety technology.
Operating or handling the instrument incorrectly
can place the user or other personnel at risk of
injury or can cause damage to the instrument or
other property. The machine may be used only as
intended and only if all of its safety features are
in proper working condition. Malfunctions that
impede safety must be remedied immediately.
To maintain this condition and ensure safe opera-
tion, the user must observe all notes and warnings
contained in these Instructions for Use.
Only original spare parts and permitted original
accessories may be used.
The CE certificate can be found on the
Internet at this website:
www.leica-microsystems.com
These Instructions for Use must be appropriately supplemented as required by the existing reg-
ulations on accident prevention and environmental safety in the operator's country.
The protective devices on both instrument and components must neither be removed nor modi-
fied. Only service personnel qualified by Leica may repair the instrument and access the instru-
ment's internal components.

10 Instructions for Use V 1.2 – 09/2012
2. Safety
2.3 Safety devices
Personal safety precautions
The instrument is equipped with the following safety devices: Handwheel lock and knife guard
on the knife holder.
The consistent use of these safety features and strict observation of the warnings and cautions
in these Instructions for Use will safeguard the operator from accidents and/or personal injury
to a great extent.
When working with cryostats, personal safety precautions must always be taken. It is
mandatory to wear work safety shoes, safety gloves, a mask and safety goggles.
2.3.2 Knife guard
The CN and CN-Z knife holders feature knife guards; the glass anti-roll plate
of the CE and CE-TC blade holders also serves as a knife guard.
2.3.1 Locking/unlocking the handwheel
12 o'clock
position
6 o'clock
position
1
2
Fig. 2
Fig. 3
Rotate the handwheel only if the refrigeration system is on and the
cryochamber is cold.
To lock the handwheel, move its handle to the 12 or 6 o'clock position. Press
the lever (1) fully outward; gently rock the handwheel back and forth until
the locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (2) on the handwheel toward the
cryostat housing.
Always lock the handwheel prior to making modifications to the
knife or specimen, changing the specimen, or taking a break.
Prior to manipulating the knife and specimen, changing the speci-
men or knife, or taking a break, always lock the handwheel and
cover the cutting edge with the knife guard!

11
Leica CM1520 – Cryostat
2. Safety
2.4 Operating conditions
Transport and installation
• Aftertransporting,donotturntheinstrumentonforaminimumof4hours.
• Donotoperatetheinstrumentinroomswithexplosionhazard.
• Toensureproperfunction,theinstrumentmustbesetupwith
the following minimum clearance to walls and fixtures on all sides:
- rear: 15 cm
- right: 30 cm
- left: 15 cm
Electrical connection
• BeforeconnectingtheinstrumenttoanACpoweroutlet,ensurethatthe
AC power of your laboratory matches the values on the nameplate of the
instrument.
• Duringthestart-upof the refrigeration system, theminimumnominal
voltage must not drop below the values specified in Chapter 3, "Technical
Data".
The compressor draws a starting current between 45 and 50 A.
Therefore, the electric circuit at the place of installation must be inspected
by an electrical engineer to ensure that it meets the requirements for a
smooth operation of the instrument.
A stable power supply in accordance with the instrument's specifications
is essential to its proper functioning.
Failure to comply with the above can damage the instrument.
•This waiting period is necessary to allow the compressor oil, which may
have been displaced during transport, to return to its original position.
Failure to comply with this may cause damage to the instrument.
• Noadditionalextensioncablemaybeused.
Defrosting
• Thequickfreezeshelfcanbecomeveryhotduringthedefrostingprocess.
Therefore, do not touch it!

12 Instructions for Use V 1.2 – 09/2012
2.5 Operating the instrument
2. Safety
2.6 Cleaning and disinfection
• Takecarewhenhandlingmicrotomeknivesanddisposableblades.Thecuttingedgeis
extremely sharp and can cause serious injuries!
• Neverleaveknivesandknifeholderswithanexposedknife/blademountedlyingaround!
• Never place a knife on a table with the cutting edge facing upward!
• Nevertrytocatchafallingknife!
• Alwaysclampthespecimenfirst,thentheknife.
• Beforehandlingthespecimenortheknife,changingthespecimenortakingabreak,lock
the handwheel and ensure that the knife is covered by the knife guard.
• Extendedskincontactwiththecoldpartsoftheinstrumentmaycausefrostbite.
• Toensurereliabledrainageofcondensateandtopreventapotentialcontaminationrisk,the
drain tube (on the side of the instrument, Fig. 52) must be inserted in a suitable catch tank.
At cryochamber temperatures of 0 °C to -30 °C. Spray a MAXIMUM of 10 sprays into the middle
of the cryostat chamber and close the window. After each disinfection, the chamber must be
wiped carefully with a cloth and briefly ventilated!
We strongly recommend defrosting the CM1520 cryostat completely for 24 hours, 1-2x annually
or as needed.
• Itisnotnecessarytoremovethemicrotomeforroutinedisinfection.Spraydisinfectionwith
Leica Cryofect is possible, thanks to the special insulation of the microtome.
We strongly recommend using the safety gloves included with the standard delivery!
Remove section waste after EVERY change of specimen. Do not start disinfection until
you have done this! Each new specimen is a potential source of contamination.
The use of flammable sprays within the cryostat chamber is permitted only with the
Leica Cryofect tested by us.

13
Leica CM1520 – Cryostat
2.7 Removing the microtome
• Themicrotomeisencapsulatedandthereforedoesnotrequireremoval
by the user.
2.8 Maintenance
Replacing the fuses
• Turntheinstrumentoffanddisconnectthepowerplugbeforereplacing
the fuses.
• Useonly the fuse types specified in Chapter 3, "Technical Data". Failure
to comply with this can cause severe damage to the instrument and its
surroundings!
Replacing the fluorescent lamp
• Turntheinstrumentoffanddisconnectthepowerplugbeforereplacing
the lamps. (More details are in Chapter 9, "Cleaning, Disinfection, Main-
tenance.)
2. Safety
• Donotuseorganicsolventsorotheraggressivesubstancesforcleaning.
Use only disinfectants listed in these Instructions for Use, such as Leica
Cryofect (alcohol or other common alcohol-based disinfectants).
For additional detailed information about disinfection, contact
Leica Biosystems.

14 Instructions for Use V 1.2 – 09/2012
3. Technical Data
Type CM1520 CM1520 CM1520 CM1520 CM1520
test symbol - c_CSA_us - - -
Nominal voltage 100 VAC ±10 % 120-127 VAC ±10 % 230 VAC ±10 % 220-230 VAC ±10 % 240 VAC ±10 %
Nominal frequency 50/60 Hz 60 Hz 60 Hz 50 Hz 50 Hz
Power input 1440 VA 1440 VA 1440 VA 1440 VA 1440 VA
Max. start-up current for 5 sec 45 A eff. 45 A eff. 45 A eff. 45 A eff. 45 A eff.
Protective class I I I I I
Pollution degree 2 2 2 2 2
Overvoltage inst. category II II II II II
Heat emission (max.) 1600 J/s 1600 J/s 1600 J/s 1600 J/s 1600 J/s
Refrigeration system 50 Hz/60 Hz
Cryochamber
Temperature range 0 °C to -30 °C (+ 3 K/- 3 K)
Cooling time down to -30 °C max. 6 hrs., at 22 °C ambient temperature
Defrost Automatic hot gas defrost
1 automatic defrost cycle/24 hours, time-controlled (duration 12 min.)
Refrigeration capacity690 W
Safety factor 3
Refrigerant 300g ±5g refrigerant R 404A *
Compressor oil 0.6 l EMKARATE RL-22S, ICI *
Quick-freeze shelf
Maximum temperature - 35 °C (+ 3 K/- 5 K)
Number of freezing stations 10
Defrost Manual hot gas defrost, time-controlled (duration 12 min.)
 according to IEC-1010, UL 3101
 according to CECOMAF Liquid temperature 45 °C, evaporation temperature -25 °C
Operating temperature range (ambient temperature): 18 °C to 35 °C. All specifications related to
temperature of the cooling unit are valid only for an ambient temperature of 22°C and a relative
humidity of no more than 60 %!

15
Leica CM1520 – Cryostat
3. Technical Data
Microtome
Rotary microtome
Section thickness adjustment: 2 - 60 µm
Total specimen feed 25 mm
Vertical stroke 59 mm
Maximum specimen size 55 x 55 mm
or 50 x 80 mm
Specimen orientation 8° (x, y, z-axis)
Electric coarse feed
Slow 600 µm/s
Rapid 900 µm/s
Cryocabinet
Dimensions
Width (w/o handwheel) 600 mm
Width (with handwheel) 730 mm
Depth 730 mm
Height 1140 mm
Weight
(incl. microtome, without specimen cooling) approx. 135 kg
Lamp
50 Hz version: Osram Dulux S 11 W/21
Color: LUMILUX light white
60 Hz version: Osram Dulux S 13 W/21
Color: LUMILUX light white
All CM1520 instrument variants need the following secondary fuses:
F1: T0.25A L250V Type FST 6.3x32 Brand: SCHURTER
F2: T1.6A L250V Type FST 6.3x32 Brand: SCHURTER
F3: T1.0A L250V Type FST 6.3x32 Brand: SCHURTER
F4: T6.25A L250V Type FST 6.3x32 Brand: SCHURTER
Storage conditions
Temperature: 5 - 50°C
Humidity: <80 %, non-condensing
Transport temperature: -40 °C to +55 °C
*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel
only.
Observe item 4.1, "Installation site
requirements".

16 Instructions for Use V 1.2 – 09/2012
4.1 Installation site requirements
4. Setting up the Instrument
The place of installation must meet the following requirements:
- The instrument requires an installation area of approx. 600 x 730 mm.
- Room volume must be at least 8 m3.
- Room temperature consistently 18°C - 35 °C,
- Temperature range during storage: 5°C - 50 °C
- Relative humidity, maximum 60% (non-condensing),
- Elevation: up to a max. of 2000 m above sea level
• Theinstrumentisdesignedforindooruseonly.
• Thepowerplug/circuitbreakermustbefreelyandeasilyaccessible.The power socket may
not be located further than 2.5 m from the instrument.
• Powersupplyatadistancenogreaterthanthelengthofthepowercable–anextension
cable must NOT be used.
• Thesubstratemustbelargelyvibration-freeandhavesufficientloadcapacityandrigidity
for the weight of the instrument.
• Avoidimpacts,directsunlight,andexcessivetemperaturefluctuations.
• TheinstrumentMUSTbeconnectedtoaproperlygroundedsocket.Useonlythepowercable
provided, which is intended for the local power supply.
• Thechemicalstobeusedareeasilyinflammableandhazardoustohealth.Thereforethe
installation location must be well ventilated, and must contain no sources of ignition of any
kind.
• Furthermore,this instrument must NOT be operated directly under the outlet of an air-
conditioning system, since the increased air circulation accelerates icing of the chamber.
• Theinstallationlocationmustbeprotectedagainstelectrostaticcharge.
Noise information: A-weighted noise level: <= 70 dB (A)
Do not operate in rooms with explosion hazard! To ensure proper function of the instru-
ment, it must be set up with a minimum distance on all sides from walls and furniture
(see "Transport and installation").
Condensation water may form in the instrument if there is an extreme difference in tem-
perature between the storage location and the installation site and if air humidity is high
at the same time. If this is the case, wait at least two hours before switching on the in-
strument.
Failure to comply with this may cause damage to the instrument.
Room temperatures and humidity levels in excess of the recommendations above will affect the
cryostat's cooling capacity and the lowest stated temperatures will not be reached.

17
Leica CM1520 – Cryostat
When the instrument is delivered, check the tilt indicators on the
packaging.
If the arrowhead is blue, the shipment was transported laying flat,
was tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for pos-
sible damage.
Fig. 4
4.2 Unpacking instructions for the CM1520 cryostat
4. Setting up the Instrument
• Lifttheoutercartonring(2) upwards and out.
• Removethefoamtransportanchors(3), and then pull the dust
cover from the instrument.
• Removetheaccessorycrate(4).
Caution when
removing the
metal strips!
There is a risk
of injury when
opening them
(the strip has
sharp edges
and is under
tension)!
To remove the metal strips (1), you
need metal shears and suitable
gloves. Stand next to the crate and
cut the strips at the location shown
(see Fig. 5 "").


Fig. 5
1
2
3 3
4
5
Fig. 6

18 Instructions for Use V 1.2 – 09/2012
4. Setting up the instrument
• Liftandremovethewoodenedge(6).
• Removetheramp(5) on the front side of the
pallet (see Fig. 6) and correctly attach it to
the back (see Fig. 8).
6


Fig. 7 Fig. 8
55
• Carefullyrolltheinstrumentbackwardsovertherampfromthepallet.
• Pushtheinstrumenttotheinstallationlocationonthecastors(7).
7
8
Transport
grip points
Do not push the device by its hood (8)!
Use the transport grip points ( , Fig. 8).
The rollers (7) must run over the ramp (5) at the front and rear. Danger of tipping!
The instrument must be transported in an upright position.

19
Leica CM1520 – Cryostat
• Whentransportingtheinstrumentonwheels,
(2) grip the cabinet only at the marked loca-
tions ( ).
• To ensure a secure uprightpositionatits in-
tended location, the instrument must be adjusted.
To do so, unscrew both adjusting feet (1) using
the provided open-end wrench (No. 13).
When subsequently transporting the instru-
ment any further on wheels, screw them back
in all the way.
1
1
2
2
2 (not visible)
Fig. 9
Transport to the site
• First,checkifthelocationmeetstheconditionsspecifiedin'Installation
site requirements'.
• Transporttheinstrumenttothedesiredlocation.
• Observethefollowing:
4. Setting up the Instrument
The instrument must be transported in an upright position or slightly
tilted (max. 30°)!
When tilting the instrument 2 people must counterbalance from the
front side to prevent the instrument from falling down and causing
severe injury, as well as possible severe damage to the instrument
and the surrounding area!

20 Instructions for Use V 1.2 – 09/2012
4.3 Standard delivery
Basic instrument in the respective voltage variant:
1 knob with marking, antibacterial............................................. 14 0491 42558
1 Set of specimen discs............................................................... 14 0470 43550
- 4 specimen discs, 25 mm ....................................................... 14 0416 19275
- 4 specimen discs, 30 mm ....................................................... 14 0370 08587
1 section waste tray .................................................................... 14 0471 30787
1 storage shelf, right .................................................................... 14 0491 46599
1 storage shelf, left ...................................................................... 14 0491 46598
1 brush shelf ................................................................................. 14 0491 46984
1 cover for freeze shelf................................................................ 14 0491 46873
1 rubber plug.................................................................................. 14 3000 00148
1 toolset:......................................................................................... 14 0436 43463
- 1 brush, fine.............................................................................. 14 0183 28642
- 1 "Leica" brush with magnet .................................................. 14 0183 40426
- 1 Allen key, no. 1.5................................................................... 14 0222 10050
- 1 Allen key, no. 2.5................................................................... 14 0222 04137
- 1 Allen key, no. 3.0................................................................... 14 0222 04138
- 1 Allen key, no. 4.0................................................................... 14 0222 04139
- 1 Allen key with spherical head, no. 4.0 .............................. 14 0222 32131
- 1 Allen key, no. 5.0................................................................... 14 0222 04140
- 1 Allen key with handle, no. 5 ............................................... 14 0194 04760
- 1 Allen key, no. 6.0................................................................... 14 0022 04141
- 1 Single-head wrench, No. 13............................................... 14 0330 33149
- 1 Single-head wrench, No. 16............................................... 14 0330 18595
1 bottle of cryostat oil, type 407, 50 ml ...................................... 14 0336 06098
1 bottle of cryocompound
(Jung tissue freezing medium) 125 ml ....................................... 14 0201 08926
1 cut-resistant safety gloves, size M......................................... 14 0340 29011
1 Instructions for Use, D/E (+ language CD) ............................ 14 0491 81001
4. Setting up the instrument
Compare the delivered components with the parts list and your order. Should
you find any discrepancies, please contact your Leica sales office without
delay.
A choice of different knife holders is available for the CM1520.