Benchmark Scientific MR-9600 User manual

SmartReader
MR-9600
User Manual
Version 3.4

Thank you for purchasing the MR-9600 microplate reader. This user manual
describes the instrument’s features, specifications, as well as complete operating
instructions, please read carefully before operation. Keep this user manual for later
use.
Please keep the box and packaging material for your microplate reader. In the event
that service is required, the box will be needed for shipping the instrument to our
Service Department.
Initial Inspection
Please check the instrument as well as all included accessories when you first open
the packaging. If you find anything damaged or missing, please contact Benchmark
Scientific or your local distributor immediately.
BENCHMARK SCIENTIFIC
PO Box 709
Edison, NJ 08818
USA
Phone: [phone]
Website: www.benchmarkscientific.com / www.accuris-usa.com
Email: [e-mail] / in[email protected]

Safety warnings and guidelines
1Important information for safe use
Users should have a clear understanding of how to use this instrument before operation, please
read this user manual carefully prior to operation.
Improper operation of this instrument may cause injury or electric shock.
Please read the manual carefully and operate safely according to the guidelines.
This instrument is for research use only!
2Operation and Maintenance
The operation and maintenance of the instrument should comply with the basic guidelines and
warnings below. Incorrect operation or maintenance will have effect on the useful life,
performance, and safety features of the instrument.
This product is a normal indoor Instrument which conforms to Standard B style- I
type- GB9706.1.
Note: Possibility of Biological Hazards. All samples for test, quality control, and
calibration should be considered infectious, and any parts of the instrument or
consumables that contact samples may also be infectious. Always wear gloves when
operating this device.
Before using the device, read the Manual carefully. These units are designed for use
in laboratory environments. The device must be used by experienced personnel
with the appropriate training.
Notice: Avoid injury. Keep your body and hands away from the motorized drawer
and any other moving parts during operation.

The operator should not open or repair the Instrument by himself. This may void
the warranty and cause damage to the instrument. If service is required, please
contact Benchmark Scientific or your local distributor to arrange repair.
Before powering on, confirm that the electrical supply available matches the voltage
required for the instrument as specified on the serial number plate. Also confirm that
the rated load of the electrical outlet is not lower than required.
If the electric line is damaged, replace it with the same type.
The Instrument should be installed in an area put in an area with minimal dust, away
from wet areas, and away from direct sunlight. In additional the installation location
should have sufficient ventilation, but away from electromagnetic interference and
heat sources. The vent on the Instrument is designed for proper cooling of the
internal components. Do not cover the vent, or place the instrument in a location
where the vent can be blocked.
Power off the instrument when not in use. If the instrument will not be used for a
long period of time, cover it with a cloth or plastic to protect it from dust.
Disconnect the power cord from the jack at once in the following cases, and contact
Benchmark or your local distributor:
⚫Liquid enters the Instrument;
⚫Abnormal operation: such as abnormal sound or smell.
⚫Instrument dropped or outer housing damaged.
⚫The function has obviously changed.
3Maintenance of the Instrument
The microplate holder should be cleaned using a cloth damp with a small amount of alcohol.
Other parts and the outer housing of the instrument can be cleaned as needed with a cloth damp
with water or a mild detergent.

Contents
Safety warnings and guidelines................................................................................................................ II
Contents..................................................................................................................................................IV
Chapter 1 Introduction........................................................................................................................ 1
Chapter 2 Features.............................................................................................................................. 2
Chapter 3 Instrument Overview.......................................................................................................... 3
Chapter 4 Installation.......................................................................................................................... 5
1. Initial check .................................................................................................................................. 5
2. Installation.................................................................................................................................... 5
3. Installation steps .......................................................................................................................... 5
Chapter 5 Operation guide.................................................................................................................. 6
Section 1 Instrument self-check.................................................................................................. 6
Section 2 Protocol interface........................................................................................................ 7
1. Keyboard function and operation ................................................................................................ 7
1.1 Keyboard function...................................................................................................................... 7
1.2 Input Buttons............................................................................................................................ 11
1.3 Running a Protocol................................................................................................................... 12
2. Protocol setting .......................................................................................................................... 13
2.1 Measure settings...................................................................................................................... 13
2.2 Layout....................................................................................................................................... 16
2.2.1 Layout............................................................................................................................ 16
2.2.2 Standard concentration................................................................................................. 18
2.3 Shake setting ............................................................................................................................ 19
2.4 Calculate................................................................................................................................... 22
2.4.1 Kinetic setting................................................................................................................ 22
2.4.2 Preprocess setting (double wavelength)....................................................................... 23
2.4.3 Curves fitting ................................................................................................................. 24
2.5 Interpret ................................................................................................................................... 25
2.6 Quality Control ......................................................................................................................... 25
Section 3 Results ....................................................................................................................... 27
1. Results interface......................................................................................................................... 27
1.1 Raw data........................................................................................................................... 27
1.2 Interpret Result ................................................................................................................ 29
1.3 Analysis............................................................................................................................. 29
1.4 Curve ................................................................................................................................ 30
1.5 QC Result.......................................................................................................................... 30
2. Kinetic Results interface............................................................................................................. 31
2.1 Kinetic Raw data............................................................................................................... 31
2.2 Kinetic Analysis................................................................................................................. 32
2.3 Kinetic Curve .................................................................................................................... 32
Section 4 Settings...................................................................................................................... 34
1. Language .................................................................................................................................... 34
2. Filter ........................................................................................................................................... 35

3. Maintenance .............................................................................................................................. 36
4. Date and Time............................................................................................................................ 37
Section 5 Help ........................................................................................................................... 38
Chapter 6 Maintenance .................................................................................................................... 39
Chapter 7 Trouble shooting............................................................................................................... 43
Chapter 8 Accessories....................................................................................................................... 44

MR-9600 Microplate Reader User Manual Chapter 1 Introduction
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Chapter 1 Introduction
This MR-9600 microplate reader is an instrument for EIA test, measuring concentration,
absorbance, measuring positive or negative reaction between antibody and antigen in samples
by reading enzymatic color change –Enzyme Linked Immunosorbent Assay (ELISA).
Key Features and Benefits:
1) Easy-to-use controls: 7 inch, color touch screen and 3 external keys.
2) Operating system allows acquisition, editing and saving of data.
3) Can be used independently from a computer, data can be saved and transferred by USB
drive.
4) 8 position optical filter wheel; 4 standard optical filters are included, additional optional
filters are available.
5) 8 channel vertical optical path, zero dispersion single mode fiber measurement system,
automatic plate well center position function.
6) 96-well visual layout allows easy setting of blank, sample, positive/negative control, quality
control and multi-value control.
7) Multi-choice tests on single plate.
8) Single or double wavelength measurement.
9) Self-checking optical path, top reading, mechanical motion.
10) Adjustable Plate shaking function, time and speed.
11) Quartz Halogen, energy saving light source.
12) Multiple ports for data export.
13) Measurement results can be exported as .CSV file, compatible with MS Excel.

MR-9600 Microplate Reader User Manual Chapter 2 Features
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Chapter 2 Features
Operating conditions
Ambient operating temperature: 4C~45C
Relative humidity: ≤70%
Input Power: AC100-240V, 50-60Hz, 2A
Specifications
Model
Parameter
MR-9600
Light source
6V, 10W, Quartz-Halogen lamp
Light source est. life
300 hours
Wavelength
340～750nm
Optical filter
Four standard filters included: 405,450,492,630nm. Filter wheel
holds up to 8 filters.
Detector Type
Silicon photodetector
Read-out range
0.000-4.000 Abs
Resolution
0.001Abs
Linearity
±1% ( 0~2.000A) , ±2% (2.000~4.000A)
Precision
CV≤0.2% (0-3Abs); CV≤1% (3-4Abs)
Accuracy (450nm)
±0.005Abs (0 ~2.0 Abs)，±1% (2.0~3.0 Abs)，±1.5% (3.0~4.0 Abs)
Channel deviation
<0.01Abs at 450nm
Measurement Speed
Single wavelength <15s/96well plate, double wavelength
<28s/96well plate (Normal speed setting)
Dimension (WXDXH)
295x440x225mm / 11.5x17x9 inches
Weight (kg)
10kg

MR-9600 Microplate Reader User Manual Chapter 3 Instrument Overview
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Chapter 3 Instrument Overview
Before first use of this instrument, please read this chapter carefully.
结构示意
There are three input buttons: “Start” ”Stop” and “Plate in/out”
Structure
Touch screen
Top front panel
Control button bar
Top rear panel
Housing
Input Buttons
Bottom plate
Plate holder
Front door
Rear panel door
USB Ports
Data port
Ethernet port
Rear door knob
Power Inlet and
Main Power Switch

MR-9600 Microplate Reader User Manual Chapter 3 Instrument Overview
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Plate holder: Holds one standard footprint, 96-well microplate.
USB Ports: For connection of keyboard, mouse and USB drive. (the upper port is for printer
connection)
Data port: for PC connection. (contact Benchmark Scientific for updated software and features)
Ethernet port: for PC connection. (contact Benchmark Scientific for updated software and features)
Rear door panel: can opened to replace light source and access optical filter wheel.
Knob: To lock and unlock the rear door.

MR-9600 Microplate Reader User Manual Chapter 4 Installation
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Chapter 4 Installation
1. Initial check
Each MR-9600 is thoroughly tested before shipping, but please check again when you receive the
instrument and contact your local distributor or manufacturer if:
⚫The outer package is damaged
⚫The outer package has any obvious moisture stains
⚫The outer package has marks of impact
⚫The outer package has signs of being opened
After opening, please check the instrument and box contents.
⚫Confirm that all ordered accessories have been included.
⚫Check the instrument’s appearance for any damage.
2. Installation
⚫Locate the instrument on a flat dry and clean work table, keeping the front side with enough space
for the plate holder to move in and out, also keep 15cm space for back, left and right side for
ventilation.
⚫Operating environment requirements:
a. The environment should be free of corrosive steam or smoke.
b. Ambient temperature should be within +4°C to +45°C.
c. Do not install in a high humidity area (Relative humidity should be from 10% to 80%).
Note: KEEP THE INSTRUMENT AWAY FROM CORROSIVE GASSES AND LIQUID!
3. Installation steps
1) Place the instrument on a stable and level surface.
2) Connect the instrument to an appropriate power outlet using the provided power cord.
Warning: Don’t connect instrument to power socket without ground wire.
3) Switch on the main power using the switch on the rear of the Instrument. The front panel will
cycle through a start-up and self-test screen.

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Chapter 5 Operation guide
Section 1 Instrument self-check
This chapter introduces default protocol operation, beginning with self-check after power on.
Refer to the picture below:
Fig 1: Start up screen

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Section 2 Protocol interface
After the self-check, the software enters into the main Protocol interface.
There are 4 tabs in the software: Protocol, Results, Settings and Help.
You can choose the tabs using the touch screen, or using a connected mouse.
Fig 2
You can create, save, measure, set parameters. Refer to Fig 2.
1. Keyboard function and operation
1.1 Keyboard function
①Current protocol name:
As shown in Fig 2, the current protocol is default Demo_1. The upper left box shows the current
protocol name to use.
②Click on to create a new protocol
Input a new protocol name, click “OK” or “return”. (Fig. 3)

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Fig 3
③Save current protocol
After creating a new name and protocol settings, click ”Save”, and the protocol will be saved to
memory.
Note: If a new protocol is used to read a plate, and data is saved, the main parameters will be
grayed out and cannot be changed. If new protocol is not used, the parameters can
be changed.
④Save as a new named protocol
After opening a current protocol, you can modify it and save it as a new one. After saving,
⑤Click to open an existing protocol.
Click “File List” button to view the list (Fig 4) of all the protocol files. Choose the protocol file and
click the “Open” button, you can view the specific information of the protocol.
Click the “”or“”button to sort the protocol files.
Click the“”,“”,“”,“”to toggle through pages.

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Fig 4
⑥Click “Batch OP”(Fig 5) to enter the interface for batch deleting, importing and exporting
protocol files.
delete the chosen protocol file (Fig 7).
Click “USB Drive”to exchange protocol files between internal disk drive
and external USB Drive (see Fig 6 to 8 ).
Export results from the internal disk to the external USB drive, files can be
exported individually or in batches.
Export the protocol files from the internal disk to external USB drive, files can be
exported individually or in batches.
Import files from the external USB drive to internal disk drive.
Select all protocol files on the internal disk or the USB drive, the button will change to
“”.
Go back to the main protocol interface.
Note: Protocol files on the external USB drive cannot be deleted using the instrument.

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Fig 5
Fig 6

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Fig 7
Fig 8
1.2 Input Buttons
Click to run a protocol
Click to stop a running protocol
Click to open or close the plate drawer

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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1.3 Running a Protocol
Measurement:
①Choose a Demo protocol, set parameters as need, or press to choose an existing a
protocol or press to set up a new protocol (see below for creating a new protocol).
②Click to open the plate holder drawer, and place the plate onto the holder.
Make sure that A1 on the plate aligns with A1 on plate holder. (see Fig 9.)
Fig 9
③Click to begin the reading of the plate. A box will appear to enter the name for the
results file.
Fig 10

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Note: During a reading, a dialog box will indicate “measuring now”, and all buttons are
disabled, except for “Stop” which can be used to interrupt the measurement.
④Measurement will take approximately 15 seconds, and upon completion the display will switch
to the “Report” interface to display measurement results (see Fig 11).
Fig 11
Results will depend on the parameters set. See section 3 on “Report Interface” for additional
information.
2. Protocol setting
After clicking or choosing one of the demo protocols, one can create a new protocol
protocol by setting the parameters (wavelength, shaking settings, layout, data calculations, etc) and the
protocol can be saved.
2.1 Measure settings
Filter (Wavelength) and mode (reading speed) can be set in the Measure settings.
①On interface “Protocol”, click on “Measure” to set the parameters. See Fig 12.

MR-9600 Microplate Reader User Manual Chapter 5 Operation guide
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Fig 12
②Filter 1 setting
Click to the right side of “Filter 1” to choose the filter wavelength. See Fig 13.
Fig 13
There are 4 standard filter included: 405nm, 450nm, 492nm, 630nm, choose a filter for the Filter 1
wavelength.
③Filter 2 Setting:
This instrument can measure at a single wavelength or two wavelengths. Click to the right side of
“Filter 2” to choose a wavelength (choose a different wavelength than Filter 1), see figure 14.