CAMAG AMD 2 User manual

INSTRUCTION MANUAL
AMD 2

Introduction
Instruction Manual, Nov-18 1
Content
1Introduction.................................................................................................................................... 2
1.1 Precaution................................................................................................................................. 2
1.2 Parts supplied............................................................................................................................ 4
1.3 Further accessories.................................................................................................................... 4
Edwards vacuum pump..................................................................................................................... 4
Vacuum connection kit ..................................................................................................................... 5
Additional metal vacuum hose.......................................................................................................... 5
Spare bottle set for AMD 2 ............................................................................................................... 5
2Unpacking/Installation .................................................................................................................. 5
3Getting started ............................................................................................................................... 7
3.1 The AMD 2 ............................................................................................................................... 7
Display and function of keys ............................................................................................................. 7
3.2 Filling the bottles ...................................................................................................................... 9
Conditioning bottle (A) ..................................................................................................................... 9
Waste bottle (B) ................................................................................................................................ 9
Solvent bottles (1-5).......................................................................................................................... 9
3.3 Initial rinsing ............................................................................................................................. 9
3.4 Insert a plate ........................................................................................................................... 10
4Application hints.......................................................................................................................... 11
4.1 Universal gradient ................................................................................................................... 11
4.2 Gradient optimization ............................................................................................................. 13
4.3 Remarks.................................................................................................................................. 13
5Maintenance and Service ............................................................................................................ 15
5.1 Cleaning ................................................................................................................................. 15
5.2 Decontamination .................................................................................................................... 15
5.3 User maintenance ................................................................................................................... 16
5.4 Service Maintenance ............................................................................................................... 17
5.5 Maintenance data sheet.......................................................................................................... 17
5.6 Troubleshooting...................................................................................................................... 18
6Technical data............................................................................................................................... 19
Declaration of Conformity (DoC)

Introduction
Instruction Manual, Nov-18 2
1Introduction
1.1 Precaution
•Please read this operating manual before starting the installation! This manual
contains information and warnings the user has to follow to ensure reliable
operation of the instrument
•If the instrument is used in a manner not specified in this manual, the protection
provided by the equipment may be impaired
•Some interior parts of the instrument are under AC power. Careless and improper
use can cause injury. Unauthorized manipulations can cause damage
•This sign indicates (on instrument and in this manual) that failure to take note of
the accompanying information may result in damage of the instrument
•The instrument is manufactured and tested in accordance with the respective
European safety publications shown on the Declaration of Conformity (DoC). The
instrument complies with safety class 1 and has been designed for indoor use only
(IP 20). Further, this device has passed the CAMAG Quality Assurance tests and has
been delivered in safe operation condition. For detailed instrument data see chapter
technical data
•Attention: For safety reasons the instrument may only be used for the purposes
described in the operating manual
•To avoid injury use adequate safety equipment (protective goggles, gloves etc. if
applicable) when working with the instrument
•Before first operation, check whether the voltage shown on the instrument
matches your local mains voltage. The power cord may only be connected to a
grounded, fused (not higher than 16 A) outlet. Do not use extension cords without
ground contact
•When working with the fluids of the instrument, be sure to take the appropriate
caution (protect your eyes from direct contact with liquid)
•The instrument may be used only by properly trained laboratory staff
•The instrument may not be used in rooms with danger of explosions
•The instrument contains highly sophisticated electronics and optical parts. It may be
operated only in a non-condensing atmosphere in the temperature range outlined
in the chapter "Technical Data". Before installation and use, the instrument should
be acclimated properly
•Use a damp lint free cloth for cleaning the instrument surface. Do not employ
aggressive detergents
•Protect yourself and the instrument from electrostatic shock which can cause
damage to the electronic parts

Introduction
Instruction Manual, Nov-18 3
•Only authorized personnel may open the instrument. Service and repair is only to
be performed by trained specialists. Use spare parts and consumables supplied by
CAMAG only. The warranty is voided if parts from other sources are used. Check
the service manual before you start service to reduce product-specific risks
•The power cord has to be removed before the instrument is opened. It is not
permitted to work on an instrument that has been opened and is connected to the
power supply
•Spare fuses must be of the type specified by the instrument manufacturer. It is
forbidden to short-circuit or manipulate fuses
•Use only the original, with the instrument delivered power cord type
•If the instrument is found to be defective, it must be switched off and steps must
be taken to ensure that it cannot be switched on by mistake
•If liquids penetrate the inside of the instrument, the power has to be disconnected
immediately. Small amounts of liquid can be wiped off and/or dried by means of a
hairdryer, with larger amounts of liquid a service technician has to be called. A test
of functionality has to be performed in all cases
•Carry out all safety checks and the preventive maintenance as recommended by the
manufacturer in order to assure your personal safety and the full functionality of
the instrument. Have an authorized service specialist perform any service not
described by this manual
•See original manufacturers' manuals for further safety data on third party
equipment supplied with the system
•Lift/move/transport the system with the necessary care and with sufficient
manpower (install the transport security devices if applicable, transport it only in the
original packaging)
•The safety of any system incorporate with the equipment is the responsibility of the
assembler of the system
•This symbol indicates that this equipment must not be disposed of as unsorted
municipal waste but is to be collected separately as electrical and electronic
equipment (WEEE-Directive 2002/96/EC). To properly recycle the instrument or
parts of it you are requested to send the equipment back to the distributor,
producer or an adequate collection system at the end of its life. This will have
potential effects on the environment and human health
•Some parts in contact with the solvent are made of anodized aluminium. Do not
use solvents or mixtures that may corrode this material (like strong acids or strong
bases) or leave residuals when evaporated. The anodized aluminium is stable
against solvents with pH between 4 and 8, provided no F-, Cl-, Br-, I- or heavy
metal ions are present

Introduction
Instruction Manual, Nov-18 4
•Not every vacuum pump is suitable for the AMD2 system. Make sure the capacity is
high enough and no oil (fume) can be drawn into the AMD2 system after the
pump has been turned off
•The screw caps of the bottles have to be carefully tightened by hand to prevent
stripping the nipple threads
•The waste air of the vacuum pump has to be connected to a fume hood or other
exhaust
1.2 Parts supplied
Part no Description
140.8860-1
1
AMD 2 accessory kit
140.0450-1
1
Pneumatic connection set II
296.0028
1
Connecting plug for vacuum pump
362.0006
2
Fuse 1 AT 5.0x20
660.0048
2
O-ring 31, 42x2, 62 FEP/MQ
662.1011
2
Lid gasket for AMD 2 chamber
663.1528
2
Silicon gasket for conditioning bottle, Ø27xØ43x5
666.1550
4
PTFE flange for conditioning bottle
700.0028
1
Spirit level, Ø 60 mm, 1°
705.0009
1
Allen key, 3 mm, chromed
705.0014
Allen key, 2,5 mm, chromed
115.8879
1
HPTLC plate positioning device
125.1028
1
Control cable, RS232 9/25Pin
B.022.8860E
1
Instruction manual
700.1011
1
CCD-Test plate ADC 2/AMD 2
115.8878
1
Plate holder
1
Power cable
1.3 Further accessories
Edwards vacuum pump
Optionally a kit containing an Edwards oil sealed rotary vane vacuum pump RV3 (2-phase
220V, operating voltage 100-120V or 220-240V selectable, 50/60 Hz) CAMAG order
#022.8880 can be supplied including following parts:
Part no Description
343.2100
1
Oil sealed rotary vane vacuum pump RV3
952.2100
1
Oil, ultra grade 15, 1 L
343.2101
1
Oil vapour filter EMF10
343.2102
1
Gas ballast/oil return kit
666.2101
1
Reducer DN25/10KF
672.3102
1
Flexible vacuum hose DN10KF, 1 m, metal
664.2101
2
Centring ring DN10KF
672.3101
2
Clamp ring DN10/16KF
666.2100
1
Tube adapter DN25KF Ø 12 mm
664.2100
3
Centring ring DN25KF
672.3100
3
Clamp ring DN20/25KF

Unpacking/Installation
Instruction Manual, Nov-18 5
Vacuum connection kit
Accessories for connecting a hose to other vacuum pumps can be supplied under the
CAMAG order #140.8885 and contains:
Part no Description
666.2102
1
Elbow (90 degrees, DN10KF)
666.2103
1
Hose connector
672.0028
1
Vacuum tube, 2m red Ø 10/20 mm
664.2101
2
Centring ring DN10KF
672.3101
2
Clamp ring DN10/16KF
Additional metal vacuum hose
Additional metal vacuum hose can be supplied under the CAMAG order #140.8880 and
contains:
Part no Description
672.3102
1
Flexible vacuum hose DN10KF, 1 m, metal
664.2101
1
Centring ring DN10KF
672.3101
1
Clamp ring DN10/16KF
Spare bottle set for AMD 2
A set of spare bottles can be supplied under the CAMAG order
#140.8865 and contains:
Part no Description
960.0064
5
250 mL bottle GL45, coated, with ETFE
pouring ring
960.0062
2
500 mL bottle GL45, coated, with ETFE
pouring ring
960.0070
7
Screw-cap GL45, blue
2Unpacking/Installation
•Take the instrument carefully out of its packaging and check the items supplied
•For installation select a smooth horizontal surface within convenient distance of the
gas supply and the vacuum pump, preferably near a fume hood. Avoid influence of
direct sunshine, heat and vibrations
•Check the position with the spirit level placed on the top of the chamber lid, when
the lid is closed. Level the instrument by turning the 4 vertically adjustable feet at
the bottom of the instrument
•Place one solvent safety trough into the recess in the rear part of the instrument lid
and slide the other in behind the lower edge of the splash protection sheet
Provide:
•External supply of compressed nitrogen or clean air, e.g. a gas cylinder with
reduction valve (4,5-6 bar, consumption about 1 L/step)

Unpacking/Installation
Instruction Manual, Nov-18 6
•A vacuum pump with sufficient capacity. If the recommended Edwards oil sealed
rotary vane vacuum pump RV3 has been supplied by CAMAG, all necessary
connections are provided. If the AMD system is to be operated with a different type
vacuum pump, note that the minimum average pumping capacity of 3 m3/h
according to PNEUROP and a final pressure of <10mbar are required. You may
need to order the additional vacuum connection kit (CAMAG order #140.8885)
Connections
•Connect the reduction valve of the gas cylinder to the intake at the back of the
instrument. Set the gas pressure to 6 bars (87psi) and open the valve of the
pressure regulator
•Remove the stoppers from the vacuum pump connections. Place the centring ring
DN25KF and the reducer DN25/10KF on the intake nipple of the vacuum pump. Fix
it with the clamp ring DN20/25KF
•Connect the flexible vacuum hose to the gas inlet of the vacuum pump by means
of the centring ring DN10KF and fix the connection with a clamp ring DN10/16KF
•Connect the other end of the flexible vacuum hose to the AMD2 vacuum
connection at the rear of the instrument. Use a centring ring DN10KF and a clamp
ring DN10/16KF
•Install the oil vapour filter EMF10 with a centring ring DN25KF and a clamp ring
DN20/25KF on the gas outlet connection of the vacuum pump
•The same centring ring and clamp can be used to fit the tube adapter DN25KF
inner Ø12 mm to the outlet side of the filter. Use a tube with an interior diameter
of at least 10 mm to connect the oil vapour filter with your fume hood
•Connect the drain-cock from the oil vapour filter with the gas ballast/oil return
using the 8 mm diameter silicon rubber tube supplied. Fix it with the plastic clamp
•Connect the tubing at the back of the AMD2 device with the respective glass
bottle. The tube marked with blue stripes is to be connected with the conditioning
bottle (A), blue connector and the tube marked yellow with the yellow connector
•Tighten all connector firmly
•Screw the red cap firmly onto waste bottle C.
•After checking that the voltage on the back of the AMD2 instrument matches your
power supply, connect the instrument to the power outlet using the power cable
supplied.
•Connect the AMD2 device to your PC via a serial port.

Getting started
Instruction Manual, Nov-18 7
3Getting started
3.1 The AMD 2
The AMD 2 instrument
The actual developing chamber is situated in the front part of the housing. Five solvent
bottles (1-5) are arranged in front. A conditioning bottle (A) and a solvent waste bottle (B)
are integrated in the hood. All valves, the gradient mixer, connecting elements and the
electronics are accommodated inside the housing.
The developing module can also operate "stand alone", i.e. the developing program last
used can be repeated as often as desired.
Display and function of keys
Control lamps
The control lamps in the display indicate the following status:
•POWER ON: this lamp is lit when the instrument is switched on.
•ON LINE: this lamp indicates a communication to the PC.
Keys
The following keys can be used:
DIALOG
Switches to the SETUP mode in which basic parameters, e.g. Baud-rate, language or LCD
contrast can be edited, and the firmware version can be displayed.
ARROW KEYS, like = upwards and = downwards
OPERATING mode: Show the current pressure and temperature.
SETUP mode: Select the parameters in the dialog.
ENTER

Getting started
Instruction Manual, Nov-18 8
OPERATING mode: Shows values of the last used method, like number of steps and used
time.
SETUP mode: Switches to the next parameter.
END
Interrupts the current development, performs a drying step and ends the program.
Pressing the END key twice interrupts also the drying step and ends the program
immediately.
In the SETUP mode this key is not active.
RESET
SETUP mode: Ends the dialog.
OPERATING mode: The running program is aborted, but the parameters remain saved in
the instrument.
In the chamber the solvent trough may remain filled with solvent. To remove the solvent
and dry the chamber, press the END key.
RUN
Display
Line1 Line 2 Description
<RUN> STARTS
AMD 2 PROGRAM
System ready message.
WAITING FOR
SYSTEM PRESSURE CLOSE
LID
System is waiting for N2-pressure or
closing of the lid.
PLATE CHECK
AND
CCD ADJUSTMENT
A brightness adjustment is per-formed
on the plate.
SYSTEM
DRAINING
The chamber is emptied; solvents are
disposed into the waste bottle.
OUTLET
OPENED
TIME
Solvent outlet is opened for the time
shown.
CANCELING
VACUUM
Chamber is equilibrated with
atmospheric pressure.
GRADIENT IS
BEING BUILT BEING
MIXED
Gradient solvents are filled into the
syringe and mixed.
VENTILATE
TIME
The chamber is equilibrated with
atmospheric pressure during the time
shown.
PRECONDITION
ING
TIME
The chamber is equilibrated with the gas
phase of the conditioning bottle during
the time shown.
PRES. TEMP.
1015 mb 25.4 °C
Shows the pressure and temperature of
the current development step or before
starting a run (after pressing the arrow
keys).
PRES. TIME
2.3 mb 2:00
Shows the pressure and drying time of
the current vacuum drying.
FRONT STEP
TIME
27.4 mm 6 8:30
Shows the current state of the
development step.
STEPS TIME
25 4:12:30
Shows the number of development steps
and the time of the last used method
(after pressing <ENTER>)
DRYING
-
Chamber is dried with vacuum.

Getting started
Instruction Manual, Nov-18 9
RINSING PASS
NUMBER
Chamber is rinsed; the number of rinsing
processes is indicated.
VACUUM TEST
-
Vacuum test is performed (before each
start).
PAUSE
TIME
Appears by pressing <RUN> during the
development. Pressing <RUN> again
continues the program.
OPERATION
COMPLETED
System ready message at the end of a
gradient.
3.2 Filling the bottles
Conditioning bottle (A)
If the AMD procedure calls for conditioning liquid, fill the conditioning bottle in position A
with at least 200 mL of the respective solvent.
The two connections marked with blue and yellow stripes should always
be fixed very tight. If you by some reason have to loosen them you must
always loosen the blue connector first! Otherwise the conditioning liquid
may be pushed up into the gas inlet pipe by the slight overpressure in the
bottle.
Waste bottle (B)
This bottle collects the waste and protects the vacuum pump for excessive solvent. Make
sure this bottle is empty before starting a new AMD 2 run.
Solvent bottles (1-5)
The solvent used to form the gradient is entered in these bottles. Depending on the
purpose of the solvent in the gradient, it can be pure or contain a mixture of several
solvents.
If acid or base has to be used in the gradient, this has to be mixed into the
individual solvent bottles and not added by the gradient mixer from one
separate bottle containing this pure acid or base.
3.3 Initial rinsing
The AMD 2 is a powerful tool for determining small amounts of impurities. Handle the
instrument, solvents and plates carefully in order to avoid trouble with false peaks
(blanks).
To avoid blanks in the chromatogram you should rinse the instrument carefully before you
run the very first gradient.