ThermoFisher Scientific QuantStudio Series User manual

For Research Use Only. Not for use in diagnostic procedures.
QuantStudio™Design and Analysis v2
USER GUIDE
for use with:
QuantStudio™7 Pro Real-Time PCR System
QuantStudio™6 Pro Real-Time PCR System
QuantStudio™5 Real-Time PCR System
QuantStudio™3 Real-Time PCR System
QuantStudio™1 Real-Time PCR System
Publication Number MAN0018202
Revision F.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision Date Description
F.0 15 April 2020 Changes for v2.4: Added analysis module information; added custom sample attributes; added
restricted editing in plate les and data les; added use analysis settings from a different le
E.0 17 March 2020 Changes for v2.3.3: Update options to edit Cqsettings
D.0 12 December 2019 Changes for v2.3: Add results report; add recommended master mix run method information
C.0 08 October 2019 Changes for v2.2: Add biogroup information; add 7900 le type; minor UI changes
B.0 26 August 2019 Changes for v2.1: Add calibration override information; embed user guide and sample plate
setup les; updated UI elements
A.0 16 April 2019 New document for v2.0
NOTICE TO PURCHASER: DISCLAIMER OF LICENSE: Purchase of this software product alone does not imply any license under any
process, instrument or other apparatus, system, composition, reagent or kit rights under patent claims owned or otherwise controlled
by Thermo Fisher Scientific, either expressly, or by estoppel.
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a
registered trademark of Roche Molecular Systems, Inc., used under permission and license. Excel is a trademark of Microsoft
Corporation.
©2020 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 About the software ........................................ 7
Compatible instruments .......................................................... 7
Compatible data les ............................................................ 7
■CHAPTER 2 Workow ................................................... 9
■CHAPTER 3 Set up a plate le ........................................ 10
Select a system template or existing plate le to set up a new plate le ............... 10
Conrm or edit run method ...................................................... 12
Run method elements ...................................................... 12
Apply the recommended run method for your master mix ....................... 12
Edit temperature ramp rate, temperature, and time length for a step ............. 13
Add or remove a step ...................................................... 13
Add or remove a stage ..................................................... 13
Add, edit, or remove a pause cycle in a PCR stage ............................. 14
Edit the ramp increment for the melt curve dissociation step .................... 15
Enable, edit, or disable Auto Delta ........................................... 15
Enable, edit, or disable VeriFlex™zones ...................................... 16
Conrm or edit lter settings ................................................ 16
Conrm or edit plate setup ...................................................... 17
Select plate wells .......................................................... 17
Add samples and assign to plate wells ....................................... 17
Add targets or SNP assays and assign to plate wells ........................... 21
Edit reagent information .................................................... 25
Select a passive reference .................................................. 25
Set up the standard curve .................................................. 26
Add biogroups and assign samples .......................................... 27
Edit plate le or data le information .............................................. 27
Review the plate le and save ................................................... 28
Export a Plate Setup le ........................................................ 28
QuantStudio™Design and Analysis v2 User Guide 3

■CHAPTER 4 Review and analyze data ................................ 29
Workow: General procedures to review the primary analysis results ................. 29
Open a data le ................................................................ 30
Review results in the Amplication Plot ........................................... 30
Evaluate the overall shape of the curves in the Amplification Plot ................ 30
Review the amplication status for each well .................................. 31
Review or edit threshold settings in the Amplication Plot ....................... 31
Review or edit baseline settings in the Amplication Plot ........................ 33
Optimize display of negative controls in the Amplication Plot ................... 33
Identify and omit outliers from analysis ........................................... 34
Review results in the Multicomponent Plot ........................................ 35
Review results in the Raw Data Plot .............................................. 36
Review results the Melt Curve Plot ............................................... 36
Review QC alerts in the Well Table ............................................... 37
Edit primary analysis settings .................................................... 37
View or edit Cqsettings ..................................................... 37
View or edit QC Alerts settings .............................................. 38
View or edit melt analysis settings ........................................... 39
Use the analysis settings from another le .................................... 39
View instrument calibration results ............................................... 40
Review ROI/Uniformity calibration results ..................................... 40
Review Background calibration results ....................................... 40
Review Dye calibration results ............................................... 41
Override the calibration data .................................................... 41
Perform additional analysis ...................................................... 42
■CHAPTER 5 Export results ............................................ 43
Export the Well Table ........................................................... 43
Export plate layout as an Excel™spreadsheet ..................................... 43
Export plate layout image ....................................................... 43
Export images of plots .......................................................... 43
Export data in the QuantStudio™le format ....................................... 44
Export data in the RDML format ................................................. 44
Generate a report .............................................................. 44
■CHAPTER 6 General procedures to analyze data in the
Quality Check tab ........................................................ 47
View the post-run summary ..................................................... 47
Congure the layout of the Quality Check tab ..................................... 47
Review individual well results in the Well Table .................................... 48
Contents
4QuantStudio™Design and Analysis v2 User Guide

Review Replicate Group results ................................................. 48
Congure general plot settings .................................................. 48
■CHAPTER 7 Manage plate les and data les ....................... 49
About system templates and plate les ........................................... 49
About data les ................................................................ 50
Save a plate le or data le ...................................................... 50
Restrict editing of a plate le or data le .......................................... 50
Add a plate le to My Plate Files ................................................. 51
Search for a plate le or data le ................................................. 51
■CHAPTER 8 Manage analysis modules .............................. 52
About analysis modules ......................................................... 52
Select an analysis module ....................................................... 53
■CHAPTER 9 Manage export settings ................................. 54
About export settings ........................................................... 54
View or edit export settings ..................................................... 54
View les in the Export Settings library ........................................... 55
Download an export settings le ................................................. 55
Import an export settings le .................................................... 56
■CHAPTER 10 About data analysis .................................... 57
About the quantication cycle (Cq)............................................... 57
About melt curve analysis ....................................................... 57
Primary analysis settings overview ............................................... 58
Cqsettings overview ....................................................... 58
Guidelines for manual threshold and baseline settings for Cqanalysis ............ 59
Melt analysis settings overview .............................................. 59
Plots overview ................................................................. 59
Amplification Plot overview ................................................. 59
Raw Data Plot overview .................................................... 60
Multi-component Plot overview .............................................. 60
Melt Curve Plot overview ................................................... 61
Contents
QuantStudio™Design and Analysis v2 User Guide 5

■APPENDIX A Troubleshooting ......................................... 62
■Documentation and support ............................................ 63
Related documentation ......................................................... 63
Customer and technical support ................................................. 63
Limited product warranty ....................................................... 63
Index ....................................................................... 64
Contents
6QuantStudio™Design and Analysis v2 User Guide

About the software
QuantStudio™Design and Analysis v2 is used to set up instrument runs on and
analyze data generated with QuantStudio™real‑time PCR systems (see “Compatible
instruments” on page 7).
Compatible instruments
Use the software to create plate les to run on the following instruments:
•QuantStudio™7 Pro Real-Time PCR System
•QuantStudio™6 Pro Real-Time PCR System
•QuantStudio™5 Real-Time PCR System
•QuantStudio™3 Real-Time PCR System
•QuantStudio™1 Real-Time PCR System
Compatible data files
The software is compatible with data les for the following real-time PCR
instruments:
•QuantStudio™7 Pro Real-Time PCR System
•QuantStudio™6 Pro Real-Time PCR System
•QuantStudio™5 Real-Time PCR System (if the plate le for the instrument run is
created using QuantStudio™Design and Analysis v2)
•QuantStudio™3 Real-Time PCR System (if the plate le for the instrument run is
created using QuantStudio™Design and Analysis v2)
•QuantStudio™1 Real-Time PCR System (if the plate le for the instrument run is
created using QuantStudio™Design and Analysis v2)
Data les for the following instruments are a legacy le format that can be opened in
the software, but can only be saved as the updated le format:
•QuantStudio™5 Real-Time PCR System (if the plate le for the instrument run
was not created using QuantStudio™Design and Analysis v2)
•QuantStudio™3 Real-Time PCR System (if the plate le for the instrument run
was not created using QuantStudio™Design and Analysis v2)
•QuantStudio™1 Real-Time PCR System (if the plate le for the instrument run
was not created using QuantStudio™Design and Analysis v2)
•QuantStudio™6 Flex Real-Time PCR System
•QuantStudio™7 Flex Real-Time PCR System
1
QuantStudio™Design and Analysis v2 User Guide 7

•QuantStudio™12K Flex Real-Time PCR System (except OpenArray™format)
•StepOnePlus™Real-Time PCR System
•ViiA™7 Real-Time PCR System
•7500/7500 Fast Real-Time PCR System
•7900HT Real-Time PCR System
Note: To convert a legacy data le into the updated le format, open the data le,
then click Actions4Save As.
Chapter 1 About the software
Compatible data files
1
8QuantStudio™Design and Analysis v2 User Guide

Workow
Set up a plate file (page 10)
Select a system template or existing plate le to set up a new
plate le (page 10)
▼
Conrm or edit run method (page 12)
▼
Conrm or edit plate setup (page 17)
▼
Review the plate le and save (page 28)
▼
Review and analyze data (page 29)
Review results in the Amplication Plot
(page 30)
▼
Identify and omit outliers from analysis (page 34)
▼
Export results (page 43)
2
QuantStudio™Design and Analysis v2 User Guide 9

Set up a plate le
Select a system template or existing plate file to set up a new plate
file
A new plate le must be created from a system template or a previously created plate
le. For more information about system templates and plate les, see “About system
templates and plate les” on page 49.
1. In the home screen, click Set Up Plate.
The Plate Gallery opens to the System Templates tab.
2. IMPORTANT! You must select a system template or a plate le that
corresponds to your instrument, block, and run mode. These properties are not
editable once the plate le has been created.
In the left pane, select the appropriate options to lter the system template and
plate le lists.
•Instrument
•Block
•Run Mode
•Analysis
Note: Thermal protocol, plate setup, and post-run analysis options are
independent of analysis selection.
3
10 QuantStudio™Design and Analysis v2 User Guide

3. Navigate to, then select a system template or plate le.
Tab Description
System
Templates
Contains system templates, non-editable plate les that are
included with the software.
Select a system template to automatically generate a new plate
le that can be edited, then saved.
My Plate Files Contains plate les that were previously saved to My Plate Files.
plate les that are included with the software.
Select an existing plate le to edit, then save, or to save as a new
plate le.
Recents Contains plate les that were recently opened. Recently opened
plate les from System Templates and My Plate Files do not
populate this tab.
Select an existing plate le to edit, then save, or to save as a new
plate le.
Note: To view all options for opening the plate le, mouse over the plate le,
then click (Actions).
The plate le opens in the Run Method tab.
Chapter 3 Set up a plate file
Select a system template or existing plate file to set up a new plate file 3
QuantStudio™Design and Analysis v2 User Guide 11

Confirm or edit run method
Edit run method elements in the Run Method tab.
2
5
3
1
4
8
7
6
9
1Stage
2Step within a stage
3Temperature ramp rate of a step
4Temperature of a step
5Time length of a step
6Add/remove stage
7Add/remove step
8Number of PCR cycles
9PCR stage pause cycle
Override the current run method with the run method that we recommend for use
with your master mix, instrument, block, and run mode. The recommended run
method can differ depending on the application being used. We recommend that you
conrm the run method in the master mix user guide.
1. On the right side of the Run Method tab, click (Actions)4Select Master
Mix.
2. Search for your master mix by name or catalog number, or select a master mix
from the list.
Run method
elements
Apply the
recommended
run method for
your master
mix
Chapter 3 Set up a plate file
Confirm or edit run method
3
12 QuantStudio™Design and Analysis v2 User Guide

3. (Optional) To add the master mix to the reagents table in the Plate Setup tab,
select Append selected master mix to reagent table.
The master mix is added to the Reagent Table in the Plate Setup tab (see “Edit
reagent information” on page 25).
4. Click Apply.
The run method is updated to the recommended run method for the selected master
mix.
1. In the Run Method tab, in the step of interest, click the temperature ramp rate,
temperature, or time length element.
For more information about elements in the run method, see “Run method
elements” on page 12.
2. Enter the value, then click outside the element to stop editing.
1. In the Run Method tab, hover over the stage for which you want to edit steps to
view the and buttons.
•The buttons at the top of the stage control adding and removing stages.
•The buttons at the bottom of the stage control adding and removing steps.
•For more information about elements in the run method, see “Run method
elements” on page 12.
2. At the insert location, click at the bottom of the stage.
3. (Optional) Edit the temperature ramp rate, temperature, or time length of the new
step (see “Edit temperature ramp rate, temperature, and time length for a step”
on page 13).
4. To remove a step, click at the bottom of the step.
1. In the Run Method tab, hover over where you want to insert the stage to view
the and buttons.
•The buttons at the top of the stage control adding and removing stages.
•The buttons at the bottom of the stage control adding and removing steps.
•For more information about elements in the run method, see “Run method
elements” on page 12.
2. At the insert location, click at the top of the stages.
Edit
temperature
ramp rate,
temperature,
and time length
for a step
Add or remove
a step
Add or remove
a stage
Chapter 3 Set up a plate file
Confirm or edit run method 3
QuantStudio™Design and Analysis v2 User Guide 13

3. Select the type of stage from the list.
Option Description
Hold •Multiple hold stages can be added.
Pre-Read •Only one pre-read stage can be added.
•A pre-read stage can only be added to the beginning of the run
method.
Post-Read •Only one post-read stage can be added.
•A post-read stage can only be added after the nal PCR stage of
the run method.
Infinite •Only one innite hold stage can be added.
•An innite hold stage can only be added to the end of the run
method.
PCR •Multiple PCR stages can be added.
Melt Curve •Multiple melt curve stages can be added.
4. (Optional) Edit the temperature ramp rate, temperature, or time length for steps
of the new stage (see “Edit temperature ramp rate, temperature, and time length
for a step” on page 13).
5. To remove a stage, hover of the stage of interest, then click at the top of the
stage.
1. In the Run Method tab, at the bottom of the PCR stage, click .
For more information about elements in the run method, see “Run method
elements” on page 12.
2. To add a pause to the PCR stage, select Pause Cycle.
3. To edit a pause, enter a pause temperature between 4°C and 99.9°C.
CAUTION! PHYSICAL INJURY HAZARD. During instrument operation, the
plate temperature can reach 100°C. To access the plate during a run pause,
enter room temperature as the pause temperature and allow the plate to cool to
room temperature before handling.
4. Enter the appropriate cycle after which the pause will occur.
5. Click outside of the Pause Setting dialog box to return to the run method.
6. (Optional) To remove a pause, click , then deselect Pause Cycle.
Add, edit, or
remove a
pause cycle in
a PCR stage
Chapter 3 Set up a plate file
Confirm or edit run method
3
14 QuantStudio™Design and Analysis v2 User Guide

1. In the Run Method tab, in the Melt stage, click the name of the ramp increment
method, then select one of the following options:
Option Description
Continuous (default) Continuously increases the temperature by the ramp
increment (°C/sec).
Step and Hold Increases the temperature by the ramp increment (°C),
then holds at that temperature for the specied time.
No. of Data Points per
Degree
Increases the temperature by the ramp increment (°C)
and collects the specied number of data points per
degree increased.
2. Edit the temperature ramp increment.
a. Click the ramp increment element in the Dissociation step.
b. Enter a value or use the up/down arrows (default is 0.15°C/s).
3. (Step and Hold only) Edit the hold time after each temperature increase.
a. Click the time eld next to Step and Hold.
b. Enter a value or use the up/down arrows (default is 5 seconds).
4. (No. of Data Points per Degree only) Edit the number of data points to be
collected with each degree increase.
a. Click the number of data points element in the Dissociation step.
b. Enter a value or use the up/down arrows (default is 10 data points).
Auto Delta enables the incremental increase or decrease of the temperature or hold
time for a step in a PCR stage.
Not all instruments support Auto Delta. For specic information about Auto Delta, see
the instrument documentation.
1. In the Run Method tab, click (Advanced Settings) in a step.
Note: Any changes apply only to the step in which you clicked.
2. In the Auto Delta Settings tab, select Enable Auto Delta.
3. Click Auto Delta Settings.
4. In the Temperature Delta eld, enter the numerical difference in the
temperature. The software indicates the appropriate range.
5. In the Time Delta eld, enter the numerical difference in the time. The software
indicates the appropriate range.
6. In the Starting Cycle eld, enter the rst cycle to which you want Auto Delta
settings to apply.
Edit the ramp
increment for
the melt curve
dissociation
step
Enable, edit, or
disable Auto
Delta
Chapter 3 Set up a plate file
Confirm or edit run method 3
QuantStudio™Design and Analysis v2 User Guide 15

7. Click Save.
A PCR step with an Auto Delta setting applied to it is denoted with .
8. (Optional) To remove Auto Delta, deselect Enable Auto delta.
VeriFlex™Zones enable independent temperature zones ≤5°C of adjacent zones.
•The number of VeriFlex™zones depends on the instrument. For specic
information about VeriFlex™zones, see the instrument documentation.
•VeriFlex™zones are only available for 96‑well blocks.
1. In the Run Method tab, click (Advanced Settings) in a step.
Note: Any changes apply only to the step in which you clicked.
2. In the VeriFlex™Zones tab, select Enable VeriFlex™.
3. In the Temperature Delta (°C) elds, enter the temperature change between
adjacent zones.
4. Click Save.
A step with VeriFlex™Zones applied to it is denoted with .
5. (Optional) To remove VeriFlex™Zones, deselect Enable VeriFlex™.
The need to edit optical lter settings is rare, and it is for advanced or custom uses
only. For more information about instrument supported dyes and their calibration and
optical lter selection, see the instrument documentation.
Use the optical lters settings to select a lter set to match the prole of a custom
dye.
1. On the right side of the Run Method tab, click (Actions)4Filter Settings.
The excitation (x) and emission (m) wavelengths that correspond to each lter
are shown on the screen.
2. Select the check boxes to enable or disable lters.
IMPORTANT! If you select the wrong lters, you cannot correct the selection
and retrieve data after a run has been completed.
3. Click Save.
Enable, edit, or
disable
VeriFlex™
zones
Confirm or edit
filter settings
Chapter 3 Set up a plate file
Confirm or edit run method
3
16 QuantStudio™Design and Analysis v2 User Guide

Confirm or edit plate setup
•Select plate wells in the (Grid View).
To Action
Select a single well Click a well in the plate
Select multiple wells Click-drag in the plate
Select contiguous wells Shift-click wells in the plate
Select non-contiguous
wells
PC: Ctrl-click wells in the plate
Mac: Cmd-click wells in the plate
Select a column of wells Click a column header
Select all wells Click the top-left corner of the plate grid
Select a block of wells Click a well to dene a corner, then shift-click another
well on the opposite corner
•Select plate wells in the (Table View).
To Action
Select a single well Click a row in the table
Select multiple wells Click-drag in the table
Select contiguous wells Shift-click rows in the table
Select non-contiguous
wells
PC: Ctrl-click rows in the table
Mac: Cmd-click rows in the table
Deselect a single well PC: Ctrl-click the selected row
Mac: Cmd-click the selected row
Import a plate setup file
Import a plate setup le that was previously exported from the software (see “Export
a Plate Setup le” on page 28), or a user-created plate setup le. The following le
types can be imported as a plate setup le:
•TXT—text format
•CSV—comma-separated values format
1. In the Plate Setup tab, in the plate layout pane, click (Actions)4Import
Plate Setup.
2. Navigate to, then select the plate setup le.
Select plate
wells
Add samples
and assign to
plate wells
Chapter 3 Set up a plate file
Confirm or edit plate setup 3
QuantStudio™Design and Analysis v2 User Guide 17

Paste samples, targets, or SNP assays in the plate layout
You can copy samples, targets, or SNP assays information from an Excel™
spreadsheet, then paste it into the plate layout grid.
1. Create an Excel™spreadsheet with the plate setup information. Refer to the
plate layout template for the correct format.
See the software Help to download a spreadsheet that includes a plate layout
template.
2. In the spreadsheet, select, then copy the cells of interest.
3. In the Plate Setup tab of the software, in the plate layout pane, click Target or
SNP.
4. In the (Grid View), select one or more wells.
5. Use Ctrl+V (PC) or Cmd+V (Mac) to paste the copied information into the plate
layout.
The copied information populates the plate layout, Samples Table, and Targets
Table or SNP Assays Table.
To complete plate setup, edit the Samples Table, and Targets Table or SNP Assays
Table if needed.
Manually add samples to the Samples table
Each sample name in the Samples table must be unique. Do not add multiple entries
for technical replicates.
1. In the Plate Setup tab, select an option in the upper right corner of the Samples
table.
Option Description
Add a single
sample
Click (Add).
Copy/paste
multiple
samples
1. Copy the sample information from one of the following
sources:
•Previously created plate le or data le—Click
(Actions)4Copy all Samples in the upper-right
corner of Samples table.
•Excel le—Select, then copy data, including column
headers.[1]
•Plate setup le—Select, then copy data, including
column headers.[1]
2. Click (Actions)4Paste Samples
[1] Column headers must match the column headers in the Samples table.
Chapter 3 Set up a plate file
Confirm or edit plate setup
3
18 QuantStudio™Design and Analysis v2 User Guide

2. (Optional) Edit the sample color and sample type (see “Edit sample color and
sample type” on page 20).
Note: The software automatically assigns a task to the target or SNP assay
based on the sample type in a well (see “(Optional) Edit the task assigned to a
target or SNP assay in one or more wells” on page 24).
3. To remove a sample from the table, click (Remove) in the last column.
Manually add or assign a sample to a well
1. In the Plate Setup tab, in the plate layout pane, select one or more wells in the
(Grid View) or the (Table View).
2. Assign a sample to the selected well.
Option Description
(Grid View)
Sample not dened Enter the sample name in the text eld.
Sample previously dened •Start typing the sample name, then
select the sample name from the
autocomplete list.
•Select the checkbox of the sample
in the Samples table.
(Table View)
Sample previously dened Select the checkbox of the sample in the
Samples table.
3. (Optional) Edit the sample color and sample type (see “Edit sample color and
sample type” on page 20).
Note: The software automatically assigns a task to the target or SNP assay
based on the sample type in a well (see “(Optional) Edit the task assigned to a
target or SNP assay in one or more wells” on page 24).
Chapter 3 Set up a plate file
Confirm or edit plate setup 3
QuantStudio™Design and Analysis v2 User Guide 19

Edit sample color and sample type
The software automatically assigns a task to the target or SNP assay based on the
sample type in a well (see “(Optional) Edit the task assigned to a target or SNP assay
in one or more wells” on page 24).
1. In the Plate Setup tab, in the Samples table, select a sample color from the
Color column dropdown list.
2. Select a sample type from the Type column dropdown list.
•Unknown (default)
•Standard
Note: A standard sample requires
that you enter a value in the
Quantity column of the Samples
table. The software uses this value
to populate the Quantity eld for
standard target tasks in the
Targets table.
•Negative Control
•Positive Control
•Positive 1/1—A sample that is
homozygous for allele 1
•Positive 2/2—A sample that is
homozygous for allele 1
•Positive 1/2—A sample that is
heterozygous for allele 1 and allele
2
Add a custom attribute to samples
1. In the Plate Setup tab, in the Samples table, click (Actions)4Add Custom
Attribute.
2. In the Add Custom Attribute window, enter the custom attribute name, then
click Done.
A column for the custom attribute is added to Samples table, and a new tab for
the custom attribute is created.
3. Select an option to dene the choices for the custom attribute:
Option Description
Dene in the Samples
table
In the Samples table, dene the custom attribute in the
appropriate eld in the custom attribute column.
Dene in the custom
attribute tab 1. In the custom attribute tab, click (Add).
2. In the table, click in the eld to edit the custom
attribute name.
The custom attribute options are added to the following locations:
•Samples table, in the dropdown list in the custom attribute column
•Table in the custom attribute tab
4. In the Samples table, for each sample, select the custom attribute from the
dropdown list.
Chapter 3 Set up a plate file
Confirm or edit plate setup
3
20 QuantStudio™Design and Analysis v2 User Guide