Benchmark Scientific Accuris MyGel InstaView User manual

Operating Manual
Version 1.0
MyGel™ InstaView
Electrophoresis Tank

Thank you for purchasing theAccuris MyGel™ InstaView Electrophoresis
Tank.
This operating manual includes a product introduction,and operating and
safety information. Before using the MyGel InstaView, please read this
manual in its entirety and be sure to fully understand the features and
methods for proper operation. Keep this manual for future reference.
Please check the list of included components in section 1.1, and if there are
any parts missing, damaged, or incorrect, please contact your distributor or
Benchmark Scientific’s Customer Service Department at 908-769-5555 or by
email at [e-mail].
Contents
1. Introduction………………………………………....................2
1.1 Included Component List…….………….…………….……..3
1.2 Diagrams of Included Components...................................4
1.3 Specifications.………………………….….............................6
2. Installation….…………………………………..……………….7
3. Warnings……………..………………………..........................7
4. Gel Preparation.……………………………….………….……8
5. Gel Tank Set Up ………………………………………………10
6. Electrophoresis ..………………………………….....…...….12
7. Viewing Electrophoresis Progress…….……….…………12
8. Imaging using a smart phone…..…….……….…..……….13
9. Maintenance……………………………………….…………..13
10. Troubleshooting………………….…..…..............................14
2
1. Introduction

The MyGel InstaView Gel Tank is a horizontal electrophoresis system that
includes a gel running tank, safety lid with viewing filter, blue LED
transilluminator, and a gel casting set. The system is designed to connect to
an appropriate electrophoresis power supply for separating nucleic acids in
agarose gels, and allows viewing the stained DNA bands during
electrophoresis without removing the gel from the tank.Accessories are
included for casting the agarose gels.
The blue LED transilluminator and viewing filter are compatible with green
fluorescent stains such as Accuris SmartGlow PS, SmartGlow LD, SYBR®
Green, and other similar stains that fluoresce under 465nm blue light.
Before use, please read this operating manual in its entirety.
1.1 Included Components
Gel Running Tank Assembly 1pc
Blue Light LED Illuminator 1 pc
12VDC Power Supply (for LED Illuminator) 1 pc
Gel Casting Stand 1 pc
Gel Casting Divider 1 pc
Long Gel Tray (10.5 x 10cm) 1 pcs
Short Gel Tray (10.5 x 6cm) 2 pcs
Double Sided Comb (12/22 teeth) 2 pcs
User Manual 1 pc
3

Safety Lid with Integral Viewing
Filter and Power Leads
Gel Running Tank
Blue LED Transilluminator
12V Power Supply
4
Smart phone imaging
enclosure with photo filter
1.2 Diagrams of Included Components

Diagram of Included Components (Gel Casting Set)
Double Sided Comb (12/22 teeth)
Long Gel Tray (10.5 x 10cm)
Short Gel Trays (10.5 x 6cm)
Gel Casting Divider
Gel Casting Stand
5

1.3 Product Specifications
Migration Tank
Dimension 120mm×110mm×45mm（inner dimension）
Buffer volume 200ml-225ml
Electrodes Platinum wire
Blue LED Transilluminator
Viewing area
dimension 10.5cm x 10.5cm
Wavelength 465nm
Input voltage 12VDC (Power Adapter included for 100VAC to
240VAC input)
Gel Casting Set
Gel casting stand Accommodates 10.5cm x 11cm and
10.5cm×6cm trays (Divider included)
Comb
specifications
Teeth width x Teeth thickness x Teeth no.）
3mm×1mm×22
5.6mm×1mm×12
Gel trays 10.5cm x 11cm and 10.5cm×6cm
6

3. Warnings
This product is capable of delivering potentially lethal voltage when
connected to a high powered power supply, and should be operated
only by qualified and trained personnel.
Please carefully read this instruction manual before operation to avoid
any personal injury. Only trained laboratory personnel should operate
the system.
Do not attempt to open or repair any parts of the system. Contact
your distributor or Benchmark Scientific for service.
Always use the MyGel InstaView in an environment with low humidity
and low dust, also keep away from water, direct sunlight / strong light,
corrosive gas, high magnetic fields, heaters, fires and other heat
sources.
Do not detach the power supply cords from the safety lid and do not
remove the safety lid when the attached power supply is running.
Attempting to do so can cause damage, and can also create a shock
hazard.
7
2. Installation
Place the electrophoresis tank on a smooth, level surface. Confirm that the power
supply to be used is suitable for the voltage and amperage requirements for DNA
electrophoresis and that the output jacks on the power supply can accept the
banana plugs on the myGel InstView power cords. The Accuris myVolt Touch and
myVolt Mini are recommended as compatible power supplies.
Use only appropriate power supplies that are designed for
electrophoresis applications, and have safety features such as no load
and over load detection.
When operating, do not move or bump the system, do not put your finger or
any other objects into the migration tank when connected to a running power
supply.
The blue light transilluminator produces a high intensity light, and users
should not stare directly at the light.

4. Gel Preparation
Place the gel casting stand on a level surface,
and place the divider and desired gel trays
into the proper positions in the stand.
Either one long gel tray, or two short trays can
be inserted into the casting stand (see
diagrams on right).
Note: If the gel tray is not level, the thickness
of the gel will not be uniform and DNA
migration may be uneven.
Insert the comb(s) into the gel casting
stand as per image on the right. Multiple
combs can be inserted depending on the
number of samples and desired run
distance.
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Note: when using the fine toothed
comb, the flat side of the well comb
should be facing the direction of
DNA travel. See figure below.

Pour the agaose gel/DNA stain
solution into the gel tray to make a
gel approximately 4mm thick.
Each 6x10.5cm tray requires
about 25ml of agarose.
Note:Accuris SmartGlow PS is
recommended for gel stain.
After the gel has solidified
(approximately 20 minutes) hold the
two sides of comb and gently lift it out
of the gel. The wells should be
straight and undamaged.
Carefully remove the gel tray with the
gel from the stand and place it into
the migration tank in the correct
orientation. (DNA samples will
migrate from the –to + electrode).
Note: After mixing and heating
agarose solution, let it cool to
approximately 60°C before
pouring into the gel trays
9

Note: Make sure that the MyGel System is
installed on a stable and level surface.
This will ensure even sample migration.
Place a prepared gel, together with the gel
tray onto the gel bed inside the gel tank.
10
Carefully pour an appropriate buffer solution to
a level of about 2 mm above the surface of the
gel. Typically, 200ml of buffer is required.
It is recommended to use Accuris QuickSilver
TAE or TBE at a concentration of 0.5X, or
QuickSilver Fast Running buffer at a
concentration of 1X for best results.
5. Gel Tank Set Up

Add DNA samples to the gel
Use an appropriate pipette to
carefully dispense samples into the
wells of the gel.
Note: Gel loading buffer should be
mixed with the DNA samples so they
will sink to the bottom of the wells.
Carefully lower the safety cover onto the
gel tank. The orange viewing filter should
fully contact the surface of the buffer so
there are no bubbles visible. If the buffer
level is too low to contact the orange
filter, remove the cover, and add more
buffer to the tank.
Slide the blue LED illuminator under
the gel tank.
11

Connect the
12V power
supply to the
LED
transilluminator
12
6. Electrophoresis
7. Viewing Electrophoresis Progress
To visualize the DNA separation in
the gel during electrophoresis, turn
on the blue LED illuminator using
the ON/OFF switch.
It may be necessary to dim or turn
off ambient lights for best viewing
results.
The LED illuminator has a built in
timer, so the light will automatically
switch off after 5 minutes.
Connect the positive (red) and negative
(black) power leads from the myGel
safety cover to the + and –outputs of a
power supply.
Set the power supply output voltage
appropriately for the application.
Generally 100V with constant voltage
setting is acceptable for most
applications. If using QuickSilver Fast
Running buffer, the voltage can be
increased to 300V for fast separation.
Connect the included 12V DC power
supply to the inlet on the InstView
transilluminator.

8. Imaging using a smart phone
To take a picture of the gel using a
smart phone camera, fit the imaging
enclosure onto the lid of the gel
running tank.
Insert the included orange photo filter
into the top platform of the enclosure.
Turn on the smart phone’s camera
application, and align the camera lens
with the photo filter.
Zoom and focus as necessary to
optimize the image, and take the
picture.
13
9. Maintenance
Always disconnect the 12V power supply when cleaning the
transilluminator. When cleaning the surfaces of the
instrument and LED transilluminator, use a damp cloth with
mild detergent if needed. Do not use any corrosive solutions
that could damage plastic.
When cleaning the gel tank, use caution when cleaning the areas
near the platinum electrodes at the bottom edges of the gel tank.
Clean gel casting stands, gel tray and combs with water or
a neutral cleaner.
Do not submerge the LED transilluminator in water.

10. Troubleshooting
Problem Causes Resolutions
No Migration
Power supply not
operating or not
correctly connected or
gel inserted backwards
Check the proper operation of the
power supply and proper connection
of the power leads to + and - outputs.
Check that the gel is properly oriented
(DNA will migrate towards + electrode)
Abnormal
Electrophoresis
speed.
Incorrect voltage
setting, incorrectly
prepared buffer.
Check power supply output, check
that buffer is the proper type and
appropriate concentration.
Abnormal heating of
buffer or gels
Buffer concentration
too high, voltage too
high, or buffer has
been reused too many
times.
Use 0.5X TAE/TBE buffer, 1X
QuickSilver Fast Running Buffer, or
reduce running voltage. If buffer has
been used in previous runs, replace
with freshly prepared buffer.
14

15
Additional Electrophoresis Products from Accuris:
QuickSilver
™ Powdered Buffer Packs
EB1000
QuickSilver TAE Buffer Powder, 100 pouches
EB1001
QuickSilver TBE Buffer Powder, 50 pouches
EB1002
QuickSilver
Fast Running Buffer Powder, 100 pouches
Benchmark Agarose LE (Molecular Biology Grade)
A1705
Benchmark Agarose LE, 500g
A1701
Benchmark Agarose LE, 100g
A1700
Benchmark Agarose LE, 25g
SmartGlow
™ Reagents
E4500
-LD
SmartGlow™ Loading Dye with Safe Green Stain, 1.0ml
E4500
-PS
SmartGlow
™ Safe Green Pre Stain, 1.0ml
Please contact your Accuris distributor for pricing and to order
myGel InstaView Accessories and Power Supplies:
Gel Casting Accessories
E1200
-CS1
Extra casting set, includes casting stand, divider, 2 trays for 10.5x6cm
gels, one tray for 10.5x10cm gels, 2 double sided combs (12/22 teeth)
E1101
-
COMB1
Extra double sided combs, 12/22 teeth, pack of 2
myVolt
™ Power Supplies
E2100
myVolt™ Mini power supply, 115V
E2100
-E
myVolt™ Mini power supply, 230V
E2301
myVolt™ Touch power supply, 115V
E2301
-E
myVolt
™ Touch power supply, 230V
Blue Light Viewing Accessories
E4000
-VG1
SmartBlue
Viewing Glasses
E1200
-OC
Orange viewing cover for
InstaView Mini Transilluminator

a division of
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