BestScope BS-2036F2B(LED) User manual

Fluorescent Biological Microscope
BS-2036F2B(LED)/F2T(LED)
Instruction Manual
BS-2036F2B(LED) BS-2036F2T(LED)
This manual is written for BS-2036F2B(LED)/F2T(LED) Series LED fluorescent biological microscope.
To ensure the safety, obtain optimum performance and to familiarize you fully with the use of this
microscope, it is recommended strongly that you study this manual thoroughly before using the
microscope and retain this manual in an easily accessible place near the work desk for future
reference.

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Contents
Microscope Body Part.....................................................................................................３
1. Pars Name ............................................................................................................３
2. Specifications .......................................................................................................４
3. Installation ...........................................................................................................５
4. Operation .............................................................................................................５
5. Image Collection ..................................................................................................６
6. Maintenance........................................................................................................７
Fluorescent Part..............................................................................................................８
1.Applications ..........................................................................................................８
2.Principle ................................................................................................................８
3.Instruction.............................................................................................................９
4.Installation ............................................................................................................９
5. Fluorescence Device Operation .......................................................................１１
Trouble shooting .........................................................................................................１３
Outfits .........................................................................................................................１４

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Attentions！！
1. Purpose.
This series microscopes are used only for microscopic observation, not available for
other purpose, otherwise result in equipment damage.
2. Disassembly only by the professionals.
The microscope has been adjusted before shipping, Unprofessional-person should not
disassemble and remove any other parts. Disassemble and remove any other parts
will result in equipment damage.
If you have any questions, please contact with manufacturer or local distributor.
3. Note the input voltage if correspond.
This instrument designed for wide input voltage (100V~240V, 50/60HZ), applicable to
most areas. If the supply voltage exceeds this range, the instrument will be seriously
damaged.
4. Prevent Burns and Fire.
When using power equipment, bulbs and collecting mirror and other nearby parts of
the set will rise sharply in temperature until it reaches a thermal equilibrium state.
Pay attention to anti-hot logo, they should be careful not be burn when in use.
Alcohol, gasoline, paper and other flammable materials can’t near the lamp in case
of fire.
5. Notes on Replacing the Bulb.
Replacement should be based on the identity of the instrument using the same
specifications of the bulb, otherwise it may cause equipment damage.
The power supply must be cut off before bulb replacement, the bulb must be cooled
off completely before proceeding! !
6. Carry.
Power must be cut off before moving. Be careful not to crush your finger when
placed.
This instrument is a precision instrument, and it should be handle with care, severe
shock can cause serious damage to related parts.
7. Installation.
Please refer to the installation instruction in order to avoid to damage the
instrument.
8. Operation Environment.
The required available environment for using of the equipment:
Indoor temperature: 0 °C ~ 40 °C , maximum relative humidity: 85%
High temperature or high humidity may cause mildew, fog or dew of the optical
components, and make the instrument not work.
9. Packing Waste Disposal
For the protection of the environment, please properly handle the microscope
packing waste or send to salvage station (such as: cardboard, foam, etc. )!
10. Statement
Our company reserves the right to improve product design and outfits.

BestScope International Limited
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Microscope Body Part
BS-2036F2B(LED)/F2T(LED) series LED fluorescent biological microscopes are designed for college
teaching, medical and clinical identification. The microscope with modern design, steady structure,
convenient operation and clear image is suitable for observing various biological specimens, they
are mostly applied in colleges and hospitals.
1. Pars Name
B
A
①
②
C_③
④
D_⑤
E_⑥
⑦
⑧
F
H
J
K
G
①
Eyepiece
②
Objective
③
Clamp
④
Mechanical Stage
⑤
Condenser
⑥
Light Collector
⑦
Main Body
⑧
Seidentopf
Binocular Head
(Trinocular Head)
⑨{
Power Input
Fuse
⑨
F
L
M
N
A
Diopter Adjustment Ring
B
Nosepiece
C
Clamp Handle
D
Handle of Iris Aperture Diaphragm
E
Field Diaphragm Ring
F
Body Handle
G
Head Fixing Screw
H
Mechanical Stage Moving Knob
J
Right Coarse & Fine Focusing Knobs
K
Potentiometer
L
Power Switch
M
Condenser Focusing Knob
N{
Tension Adjustment Ring
Left Coarse & Fine Focusing Knobs

BestScope International Limited
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2. Specifications
2.1 Total magnifications
Objective
Total Magnifications
Eyepiece
4X
10X
20X
(Optional)
40X
100X
10X
40X
100X
200X
400X
1000X
16X
64X
160X
320X
640X
1600X
1.2 Objectives
Infinite Plan Objectives are optional.
Infinite Plan
Objectives
Numerical
Aperture (N.A.)
Objective
Field
Resolution
(μm)
Working
Distance(mm)
4X
0.10
5mm
3.35
12.1
10X
0.25
2mm
1.34
4.64
20X (Optional)
0.40
1mm
0.83
2.41
40X (S)
0.65
0.5mm
0.50
0.65
60X (S) (Optional)
0.80
0.33mm
0.41
0.33
100X (Oil) (S)
1.25
0.2mm
0.26
0.12
1.3 The other specification
2.3.1 Mechanical tube length: 180mm
2.3.2 Conjugate distance: Infinity
2.3.3 Head: Seidentopf Binocular or Trinocular, Inclined 30°, Rotatable 360°, Anti-fungal
systems. Interpupillary Adjustable Distance Is 48-75mm, Diopter adjustable range ±5.
2.3.4 Nosepiece: Quadplex nosepiece
2.3.5 Mechanical stage: Size 145mm×140mm, X-Y travel 76mm×52mm
2.3.6 Focusing systems: Coaxial Coarse and Fine Focusing Knobs, Coarse stroke 26mm,
Fine division 2µm, Condenser up-down range 22mm
2.3.7 Condenser: Abbe condenser, N.A. 1.25, Adjustable aperture, Aperture center can be
adjustable.
2.3.8 Illumination: Non-spherical System
2.3.9 Filter: Built in blue filer
2.3.10 Electric components: Input voltage AC85-265V/LED
Output voltage DC1.2-6V/LED,
Rotation potentiometer with power switch, Fuse 2A ф5×20

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Condenser
screw
Condenser
installing ring
Condenser
3. Installation
Please clean the operation desk before installation. Put out the microscope of the carton and put it
on the desk.
Make sure the supply voltage meets the instrument’s requirement and the power switch is off.
Installation Instruction Fig.:
1.Turn the binocular (trinocular) head to working position;
2.Put out the dust cover of the eyepiece tube;
3.Insert into the eyepiece;
4.Install Abbe Condenser.
3.1 Eyepiece Tube:
Loose the Head Fixing Screw G, turn the tube to observing
position, then tighten the Screw G.
3.2 Put out the eyepiece dust cover.
3.3 Eyepiece
Put out the eyepiece of the carton, and insert it into the
tube. Please don’t touch the lens of the eyepiece by hand.
3.4 Condenser
Put out the condenser of the carton, then turn
Condenser Focusing Knob M to lower condenser
installing ring. Loose the condenser screw, then
install the condenser, and make the condenser
graduation face to the front, Tighten the condenser
screw, and higher the condenser installing ring to
top. Please don’t touch the lens by hand.
3.5 Power
Put in power, open Switch, and turn Potentiometer.
4. Operation
1
2
3
4

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4.1 Bright field operation process instruction
5. Image Collection
5.1 Installing
Connect the C-mount with CCD camera or connect camera with camera adapter, then connect it
with c-mount, finally put it into microscope.
5.2 Using
First get a clear image from eyepiece, then put out lever on the side of trinocular head, and collect
image with camera. Clear image should be in screen. Adjust B14 fine focusing knobs to get it clear if
image is not clear.
5) Adjust condenser aperture
diaphragm
Adjust condenser ⑤(condenser focusing
screw M) and aperture (Aperture diaphragm
lever D)
Observing
1) Turn power switch,
adjust light
Turn on Switch L, and turn Potentiometer K.
2) Put the sample
Put the sample on stage ④, (open the clamp
C, and put the sample into it), turn stage knob
H to put the sample into optical path.
3) Put 10X objective into
optical path and focus
Turn Nosepiece B, put 10×objective into
optical path. Turn Coarse & Fine Focusing
Knobs I and N to focus.
4) Adjust interpupillary
distance and diopter
Adjust head ⑧to make two fields into one;
adjust diopter adjuster A to make diopter for
two eyes same.
6) Adjust and center field
diaphragm
Adjust field diaphragm
(field diaphragm ring E)
Put the objective into
light path, and focus

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6. Maintenance
6.1 Clean microscope
6.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft brush or absorbent
cotton or cleaned by absorbent cotton, lens paper with the mixture of alcohol and ether
(proportion 1:4).
6.1.2 Alcohol and ether all are burnt early, please take them away from fire. Be careful for turn on
and off power.
6.1.3 Don’t clean painted metal and galvanizing metal with organic solvent such as alcohol, ether or
the mixture of the both. Silicon cloth or soft cleaning preparation is suggested to clean it.
6.1.4 Plastic should be cleaned by soft cloth with clear water.
6.2 Environment of using and placing
6.2.1 Microscope should be used and placed in a cool, dry, non-dust, non-shake and non-corrosive
gases environment.
6.2.2 Microscope should be used in environment of indoor temperature 0°-40°C and maximum
relative humidity 85%.
6.2.3 Removing equipment is suggested to be installed when microscope used in heavy humidity
area to avoid fungus and mist damage instrument.
6.2.4 Please pay attention to prevent microscope from violent shake and vibration in application
and in carrying. Don’t drag it on the surface of worktable to avoid damage to microscope and
worktable.
6.3 Replacement of bulb
6.3.1 Turn off power, and pull out plug.
6.3.2 Wait the bulb become cool.
▲Please be sure that the bulb is cool, then follow by
the nest operations.
6.3.3 Lay aside the microscope reliably, unscrew the
knurled thumb screw of the lamp housing cover on the
underside of base.
6.3.4 Pull over the lamp housing cover.
6.3.5 Pull out the bulb should be replaced, hold a new
bulb with silk cloth to avoid fingerprint and dust affect bulb brightness and service life, and insert
fully the contact pins into the bulb socket.
6.3.6 Close the lamp housing cover, and screw the knurled thumb screw.
▲After working for above 10 hours continuously, better
cut off the microscope about 30 minutes.
6.4 Replacement of fuse
6.4.1 Cut off power of microscope, pull out the plug.
6.4.2 Unscrew fuse cap in the back of base, take out old
fuse.
6.4.3 Replace a new fuse, then screw the fuse cap.

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Fluorescent Part
1.Applications
Some objects can emit a ray which wavelength is longer than that of the excitation light when
irradiated. This ray is called fluorescence, and observers can study the objectives through
fluorescence microscope using the phenomenon.
Fluorescence microscope has wide applications in basic theory research and clinical diagnosis
about medicine, biology, as well as analysis and test in industry, agriculture, stockbreeding,
criminal investigation, legal medical appraise, environmental protection etc.
2.Principle
The device consisting of
Epi-fluorescence illuminator,
5W LED lamp, fluorescence
objectives is combined with
microscope to make up
fluorescence microscope. The
device is designed and
manufactured with
Epi-excitation principle
and standard provided with
two group excitation filters
system: blue (B), green (G).
The light emitted from the
lamp is converted to the
excitation light (e.g.blue light)
with specified wavelength by
going through the excitation filter, then passes through dichroic prism and objectives (the
objective plays role of condenser) to irradiate vertically the object. The object is excitated and
emits fluorescence with specified wavelength (e.g. green and yellow) and make image passing
through objectives, dichroic prism and eyepieces. The light (including excitation light) without
fluorescence wavelength is reflected or absorbed by dichroic prism and barrier filter, and can
not reach the view system. Therefore, what can be seen in the view field is the bright
fluorescence image against the dark background.
Fluorescence Device Diagram
Fluorescence
module
Condenser
prism
LED
lamp
Barrier filter
Dichroic
prism
Excitation filter
Eyepiece
Objective
Specimen

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3.Instruction
The fluorescence microscope is building-block structure, it includes main body, fluorescence
conversion device, fluorescence excitation modules, fluorescence light source lamphouse and
objective etc. Fluorescence device with 2 position can install two excitation modules and bright
field observation position. Standard outfits: FL2-LED and special LED device for single. In
addition, other excitation modules can be used as per user’s requirements.
FL2-LED two-groups fluorescence device with LED illumination, it has excellent feature, without
waiting startup time for lamp, low voltage, no fever on lamp house etc. Standard equipped with
B,G two group fluorescence modules. Single LED fluorescence device is designed for single
module, customize for especial requirement.
4.Installation
Two-Groups LED Fluorescence Device Installation Marking Graphs
LED Fluorescence Device Installation Marking Graphs
18
19
Ordinary LED Power Box
16
17
14
15
20
17
21
22
Lithium power source
14
15

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14, 15 double ended connector wire for lamp box and power supply
16 two group LED light box
17 light box power socket
18 external power port
19 LED power box output socket
20 single group LED fluorescence light box
21 output socket of lithium battery power box
22 external power connector
1）Take all parts from the package, remove the protective package and place it on the vacant
bench.
2）Turn fluorescence device and put the protective plate in with the screw, then tighten it by
wrench.
3）Then put the device in upright direction, then link the main body connector with main body
bayonet, fasten the microscope with screw.
4）Slightly loosen the locking screw, then connect the tube connector on the front and the
back-tube of the fluorescence power box, fixed with locking screw.
5）Joint the observing tube with bayonet, and then lock it with wrench.
6）Use lamp power line to connect lamp power socket and relevant socket.
7）According to the installation instruction to install microscope main body.
▲Rechargeable Lithium power source specially for LED illumination.

BestScope International Limited
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5. Fluorescence Device Operation
Please adjust microscope in bright field method and operate fluorescence attachment as follows:
LED Fluorescence Device Operational Parts Marking Graphs
20 Lamp condenser adjusting lever 21 LED switch shift lever
22 brightness adjusting knob 23 power switch
24 charging indicator 25 fuse tube
( Single group LED lamp source is similar as two group lamp source)
1) Insert the plug of the power source into external power supply socket (please make sure the
rated current and voltage are coincided with the input supply voltage at first).
2) Turn off the Epi-fluorescence switch and turn on the power source (the input voltage should be
within 100V-220V).
3) Put 10X fluorescence objective in the optical path and lower transmission condenser to a
minimum or take off.
4) Place the fluorescence specimen on the stage and fix it with the clamp, adjust the stage knobs
to move the specimen in the optical path.
5) Pull the filter converting lever to the needed position.
6) Maximize the field diaphragm by manipulating the field diaphragm adjust lever on the
Epi-fluorescence device.
7) Focusing by rotating the coarse and fine focusing knobs to make the image clear.
8) To observe with other magnification objectives after getting ideal image.
9) When use multigroup light source, can choose just compare with module conversion position
witch in front of the fluorescence device .
10) Rechargeable Lithium power source specially for LED illumination can be used above 10 hours
continuously without external power supply. When it is charging, the indicator is dark as the
battery capacity lower than 80%, and the indicator is flashing as the battery capacity is 80%.
The indicator is bright as the battery capacity is full. The indicator comes dark after the external
power supply cut.
21
20
22
23
24
25

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▲Before perform fluorescence observation, locate the specimen with the transmission light first.
▲To prevent the fluorescence from attenuation quickly, block the excitation light with barrier
when preparing for fluorescence observation or photography. Only when observing or
photographing, irradiate the specimen with the excitation light.
▲Fluorescence microphotograph requires a long exposure time, so it is better to use the high
sensitivity camera.
▲Ordinary LED Power Box and Lithium power source without start button.
▲Operation Marking Instruction
|
ON
FD
Field Diaphragm
○
OFF
AD
Aperture Diaphragm
Direction Switch for Diaphragm, Hollow Round means big, non-hollow round
means small.
No Light pass
Attentions
50% light pass
High temperature
Shut down the power and wait until the
lamp cool when replace lamp.
100% light pass

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Trouble shooting
In the period of using this series microscope, if there is any trouble occurs, please referring to the
following sheet listed some common troubleshooting resolve them.
Trouble
Causation
Remedy
Switch on but no light
Plug is unreliable
Plug in again
Fuse is broken
Change fuse
Brightness of view field isn’t
enough or is Uneven
Light doesn’t meet the requirement
Adjust the light intensity
Brightness isn’t adjusted correctly
Adjust rotation potentiometer
Objective isn’t in correct position
Make the objective in correct position
The size of iris aperture is too small
Adjust the size of iris aperture
Brightness of view field isn’t
enough or is Uneven
Lens (objective, eyepiece, condenser,
light collector ) has dust
Clean it
Position of condenser is too low
Higher condenser
Image isn’t clear
(contrast or definition isn’t
enough)
Cover glass of specimen doesn’t meet
the requirement
Use required thickness cover glass
(0.17mm)
Cover glass of specimen isn’t in up
direction
Place specimen correctly
Surface of objective lens is dirty
(especially it is easy for the front lens of
40X objective to dip in immersion oil)
Clean it
Immersion oil isn’t used for 100X
objective (oil)
Use immersion oil
Immersion oil doesn’t meet the
requirement
Use immersion oil supplied by us
There is bubble in immersion oil
Clear the bubble way
Size of iris aperture isn’t proper
Adjust the size of iris aperture
Position of condenser is too low
Readjust the position of condenser
One side of image is
dark or image is
moving as focusing
Objective isn’t in correct position
Make the objective in
correct position
Specimen isn’t placed correctly
Place specimen levelly on stage and clip
it with clamp
Objective touches
specimen as changing low
times objective to high
times objective
Cover glass of specimen isn’t in up
direction
Place specimen correctly
Cover glass doesn’t meet the
requirement
Use required thickness
cover glass (0.17mm)
Image observed by two
eyes aren’t in
superposition entirely.
Interpupillary distance isn’t adjusted
correctly
Adjust interpupillary distance according
to two eyes
It is easy for eyes to be tired
during observing
Diopter isn’t adjusted correctly
Readjust diopter

BestScope International Limited
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Outfits
Item
Specification
BS-2036
F2B(LED)
BS-2036
F2T(LED)
Optical System
Infinite Optical System
●
●
Viewing Head
Seidentopf Binocular Viewing Head, Inclined at 30°, 360°
Rotatable, Interpupillary 48-75mm
●
Seidentopf Trinocular Viewing Head, Inclined at 30°, 360°
Rotatable, Interpupillary 48-75mm, Light Distribution 20:80
●
Eyepiece
WF10×/20mm
●
●
WF16×/13mm
○
○
Reticule Eyepiece WF10×/20mm (0.1mm)
○
○
Objective
Infinite Plan Achromatic Objective 4×, 10×, 40×, 100×(Oil)
●
●
Infinite Plan Achromatic Objective 20×, 60× (S), 100× (Dry, S)
○
○
Infinite UPlan fluorescent objective 10×, 20×, 40×, 100×
○
○
Nosepiece
Backward Quadruple Nosepiece
●
●
Backward Quintuple Nosepiece
○
○
Focusing
Coaxial Coarse & Fine Focusing knobs, Travel Range: 26mm,
Scale:2um
●
●
Stage
Stage Size: 145×140mm, Cross Travel 76×52mm, Scale0.1mm,
Two Slide Holder
●
●
Rackless Double Layers Mechanical Stage, Size: 140×135mm,
Cross Travel 75×35mm, Scale0.1mm, Two Slide Holder
○
○
Condenser
Abbe Condenser NA1.25 with Iris Diaphragm
●
●
Transmitted
Illumination
3W-LED Illumination, Brightness Adjustable
●
●
12V/20W Halogen Lamp, Brightness Adjustable
○
○
6V/30W Halogen Lamp, Brightness Adjustable
○
○
Fluorescence
Attachment
Epi fluorescence unit ( 2 positions for Uv /V/B/G fluorescent
filters), 5W-LED fluorescence lamp
●
●
Fluorescent
filters
Excitation
Dichroic Mirror
Barrier Filter
Blue
excitation
420-490
505
520
●
●
Green
excitation
500-550
575
590
●
●
Ultraviolet
Excitation
330-380
400
435
○
○
Violet
Excitation
380-420
430
460
○
○
Filter
Blue /Green /Yellow
○
○
Optional
Accessories
Field Diaphragm, Photo adapters(for DSLR cameras), Video
adapters(1X, 0.5X), Polarization set, Phase contrast kit, mirror,
○
○

BestScope International Limited
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Cable Winding Device
Package
42cm*28cm*45cm, 40cm*20cm*40cm, 12kg
●
●
Note: ●Standard Outfit, ○Optional