Binder CB 60-UL User manual

Issue 07/2017 Art. No. 7001-0333
Operating Manual
CB (E6.1)
CO2- Incubators
CO2– Incubators with O2control
with sterilizable NDIR sensor system for CO2
and microprocessor controller T4.12
Model Voltage Equipment Art. No.
CB 60 230 V 9040-0088, 9140-0088
CB 60-UL
100-120V
9040-0089, 9140-0089
CB 60
230 V
O2control
9040-0090, 9140-0090
CB 60-UL
100-120V
O2control
9040-0091, 9140-0091
CB 60
230 V
Divided inner door
9040-0104, 9140-0104
CB 60-UL
100-120V
Divided inner door
9040-0105, 9140-0105
CB 60
230 V
O2control
Divided inner door
9040-0106, 9140-0106
CB 60-UL
100-120V
O2control
Divided inner door
9040-0107, 9140-0107
CB 160 (E6)
230 V
9040-0092, 9140-0092
CB 160-UL
100-120V
9040-0093, 9140-0093
CB 160 (E6)
230 V
O2control
9040-0094, 9140-0094
CB 160-UL
100-120V
O2control
9040-0095, 9140-0095
CB 160
230 V
Divided inner door
9040-0100, 9140-0100
CB 160-UL
100-120V
Divided inner door
9040-0101, 9140-0101
CB 160
230 V
O2control
Divided inner door
9040-0102, 9140-0102
CB 160-UL
100-120V
O2control
Divided inner door
9040-0103, 9140-0103
CB 220
230 V
9040-0096, 9140-0096
CB 220-UL
100-120V
9040-0097, 9140-0097
CB 220
230 V
O2control
9040-0098, 9140-0098
CB 220-UL
100-120V
O2control
9040-0099, 9140-0099
CB 220
230 V
Divided inner door
9040-0108, 9140-0108
CB 220-UL
100-120V
Divided inner door
9040-0109, 9140-0109
CB 220
230 V
O2control
Divided inner door
9040-0110, 9140-0110
CB 220-UL
100-120V
O2control
Divided inner door
9040-0111, 9140-0111
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

CB (E6.1) 07/2017 Page 2/145
Contents
1. SAFETY..................................................................................................................6
1.1 Legal considerations...........................................................................................................................6
1.2 Structure of the safety instructions......................................................................................................6
1.2.1 Signal word panel .....................................................................................................................6
1.2.2 Safety alert symbol ...................................................................................................................7
1.2.3 Pictograms................................................................................................................................7
1.2.4 Word message panel structure.................................................................................................8
1.3 Localization / position of safety labels on the chamber ......................................................................8
1.4 Type plate .........................................................................................................................................10
1.5 General safety instructions on installing and operating the CO2incubator ......................................11
1.6 Precautions when working with gases..............................................................................................12
1.7 Precautions when handling gas cylinders.........................................................................................14
1.8 Intended use .....................................................................................................................................15
2. CHAMBER DESCRIPTION..................................................................................16
2.1 Chamber overview............................................................................................................................17
2.2 Instrument panel ...............................................................................................................................17
2.3 Inner chamber...................................................................................................................................18
2.4 Control panel on the rear of the chamber.........................................................................................20
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................21
3.1 Unpacking, and checking equipment and completeness of delivery................................................21
3.2 Guidelines for safe lifting and transportation.....................................................................................21
3.3 Storage..............................................................................................................................................22
3.4 Location of installation and ambient conditions ................................................................................22
4. INSTALLATION AND CONNECTIONS ...............................................................26
4.1 Shelves..............................................................................................................................................26
4.2 Permadry™ water pan......................................................................................................................26
4.3 Connecting the O2sensor (chamber with O2control).......................................................................27
4.4 Gas connections ...............................................................................................................................28
4.4.1 Connection of the CO2gas cylinder........................................................................................29
4.4.2 Connection of the O2gas cylinder (chamber with O2control and optional alternative control
range 10 up to 95 vol.-% O2) ..................................................................................................30
4.4.3 Connection of the N2gas cylinder (chamber with O2control).................................................31
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)............................................32
4.4.5 Gas cylinder connection kits (option)......................................................................................33
4.5 Electrical connection.........................................................................................................................34
4.6 Handling and aligning the divided inner door, gas proof (optional equipment).................................35
5. START UP............................................................................................................36
5.1 Equilibration time...............................................................................................................................36
5.2 Factory settings.................................................................................................................................36
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER.............37
6.1 Menu structure..................................................................................................................................38
6.1.1 General menu.........................................................................................................................38
6.1.2 Quick menu.............................................................................................................................39
6.1.3 User menu ..............................................................................................................................40
6.2 Operating modes...............................................................................................................................41
6.2.1 Activating the “control off”mode or change to “fixed value” operating mode.........................41
6.3 Deactivating the O2control and O2/N2pressure alarms (chamber with O2control) .........................43
6.3.1 Required gas supply of the chamber with O2control .............................................................43
6.3.2 Activating / deactivating the O2control and O2/ N2pressure alarms.....................................44

CB (E6.1) 07/2017 Page 3/145
6.4 Humidity control of the Permadry™ system......................................................................................45
6.5 Turning on / off the interior socket voltage (with optional interior socket).........................................46
6.6 Performance during and after power failure......................................................................................46
6.7 Information........................................................................................................................................47
7. SETPOINT ENTRY...............................................................................................48
7.1 Setting ranges...................................................................................................................................48
7.2 Note when setting high gas concentrations......................................................................................48
7.3 Entering the setpoints via “quick menu”............................................................................................50
7.4 Entering the setpoints via general menu...........................................................................................52
8. KEY LOCK ...........................................................................................................54
8.1 Directly activating the key lock..........................................................................................................55
8.2 Automatic key lock............................................................................................................................55
8.3 Changing the password for unlocking the key lock...........................................................................57
9. GENERAL CONTROLLER SETTINGS................................................................58
9.1 Setup wizard .....................................................................................................................................59
9.2 Date and time settings ......................................................................................................................59
9.3 Selecting the menu language of the T4.12 controller.......................................................................61
9.4 Setting display brightness.................................................................................................................61
9.5 Changing the temperature unit .........................................................................................................62
9.6 Defining the data recording rate........................................................................................................62
9.7 Factory reset .....................................................................................................................................63
9.8 Network configuration .......................................................................................................................63
9.9 Display of the network configuration.................................................................................................67
9.10 RS 422 address (with optional RS 422 interface).............................................................................68
9.11 Display and entry of the device configuration – for service purpose................................................68
10. DATA TRANSFER VIA USB INTERFACE ..........................................................69
10.1 Exporting data to USB drive..............................................................................................................69
10.2 Importing data from USB drive..........................................................................................................70
11. NOTIFICATIONS AND ALARMS.........................................................................71
11.1 Notifications overview .......................................................................................................................71
11.2 Alarm messages overview................................................................................................................71
11.3 Alarm status ......................................................................................................................................73
11.4 Confirming an active “set” alarm.......................................................................................................74
11.5 Alarm configuration and overview.....................................................................................................75
11.5.1 List of active alarms................................................................................................................75
11.5.2 History – list of all alarms........................................................................................................76
11.5.3 Activating, deactivating, and testing the alarm buzzer ...........................................................77
11.5.4 Activating / deactivating all alarm functions............................................................................77
11.5.5 Setting the delay time after opening the door.........................................................................78
11.5.6 Tolerance ranges and alarm delay times ...............................................................................78
11.6 Zero-voltage relay alarm output........................................................................................................82
12. EVENT LIST.........................................................................................................83
13. GRAPHICAL DISPLAY OF THE MEASURED VALUES.....................................84
13.1 Setting the sampling rate..................................................................................................................84
13.2 Defining the display range.................................................................................................................85
13.3 Selecting the parameters..................................................................................................................86
14. TEMPERATURE SAFETY DEVICES...................................................................87
14.1 Overtemperature protective device (class 1)....................................................................................87
14.2 Overtemperature safety controller (temperature safety device class 3.1)........................................87
14.2.1 Safety controller modes..........................................................................................................87
14.2.2 Setting the safety controller....................................................................................................88

CB (E6.1) 07/2017 Page 4/145
15. OPTIONS..............................................................................................................91
15.1 Communication software APT-COM™ 3 DataControlSystem (option).............................................91
15.2 RS 422 interface (option)..................................................................................................................91
15.3 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)............91
15.4 Interior socket 230V (option).............................................................................................................92
15.5 Analog outputs for temperature and CO2(option) ............................................................................94
15.6 Access port for extra-low voltage (option).........................................................................................94
15.7 BINDER Gas Supply Service – External bottle changer for CO2, N2or O2(option).........................95
15.8 Stands...............................................................................................................................................96
15.8.1 Stacking stand (option)...........................................................................................................96
15.8.2 Stacking adapter for direct thermal decoupled stacking (option) ...........................................96
15.8.3 Base on castors (option).........................................................................................................96
16. REFERENCE MEASUREMENTS ........................................................................97
16.1 CO2reference measuring .................................................................................................................97
16.1.1 Measuring CO2concentration indirectly via the pH of the cell medium..................................97
16.1.2 Measuring CO2directly via chemical indicator tubes .............................................................98
16.1.3 Measuring CO2directly with an electronic infrared measuring device...................................99
16.2 Temperature reference measurement..............................................................................................99
17. AVOIDING MICROBIAL CONTAMINATION........................................................99
17.1 Cells and media ................................................................................................................................99
17.2 Laboratory conditions / equipment around the incubator..................................................................99
17.3 Working and behavior in the lab .....................................................................................................100
17.4 Chamber design and equipment of the CO2incubator...................................................................100
17.5 Handling the CO2incubator ............................................................................................................101
18. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION..103
18.1 Cleaning..........................................................................................................................................103
18.2 Decontamination / chemical disinfection of the chamber................................................................105
18.3 Hot-air sterilization at 180 °C / 356 °F.............................................................................................106
18.3.1 Overview...............................................................................................................................106
18.3.2 Performing a hot-air sterilization...........................................................................................107
18.3.3 Aborting the hot-air sterilization – general procedure...........................................................109
18.3.4 Aborting hot-air sterilization after less than 4 hours .............................................................110
18.3.5 Terminating successful sterilization during the cooling-down phase (abortion after more than
4 hours).................................................................................................................................112
18.3.6 Terminating the completed sterilization cycle.......................................................................113
19. MAINTENANCE AND SERVICE........................................................................115
19.1 Maintenance intervals, service........................................................................................................115
19.2 Checking the air jacket heating fan.................................................................................................116
19.3 Checking the humidity system fan..................................................................................................116
19.4 Gas inlet fine filter ...........................................................................................................................116
19.5 Sending the chamber back to BINDER GmbH...............................................................................116
20. DISPOSAL .........................................................................................................117
20.1 Disposal of the transport packing....................................................................................................117
20.1.1 Outer chamber packing ........................................................................................................117
20.1.2 Packing inside the chamber and equipment.........................................................................117
20.2 Decommissioning............................................................................................................................118
20.3 Disposal of the chamber in the Federal Republic of Germany.......................................................118
20.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................119
20.5 Disposal of the chamber in non-member states of the EU.............................................................120

CB (E6.1) 07/2017 Page 5/145
21. TROUBLESHOOTING .......................................................................................121
21.1 General............................................................................................................................................121
21.2 Heating............................................................................................................................................121
21.3 Gas cylinder pressure too low.........................................................................................................123
21.4 Gas concentration...........................................................................................................................124
21.5 Sterilization......................................................................................................................................126
21.6 Humidity ..........................................................................................................................................126
21.7 Controller.........................................................................................................................................127
21.8 Open door.......................................................................................................................................127
22. TECHNICAL DESCRIPTION..............................................................................128
22.1 Factory calibration and adjustment.................................................................................................128
22.2 Over current protection ...................................................................................................................128
22.3 Definition of usable volume.............................................................................................................128
22.4 CB technical data............................................................................................................................129
22.5 Equipment and Options...................................................................................................................131
22.6 Accessories and spare parts (extract) ............................................................................................132
22.7 Important conversion data for non-SI units.....................................................................................133
22.8 Conversion table for gas inlet pressures, bar – psi.........................................................................133
22.9 Dimensions CB 60 ..........................................................................................................................134
22.10 Dimensions CB 160 ........................................................................................................................135
22.11 Dimensions CB 220 ........................................................................................................................136
23. CERTIFICATES AND DECLARATIONS OF CONFORMITY.............................137
23.1 EU Declaration of conformity ..........................................................................................................137
24. PRODUCT REGISTRATION..............................................................................139
25. CONTAMINATION CLEARANCE CERTIFICATE .............................................140
25.1 For chambers located outside the USA and Canada .....................................................................140
25.2 For chambers in the USA and Canada...........................................................................................143

CB (E6.1) 07/2017 Page 6/145
Dear customer,
For the correct operation of the CO2incubator CB, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor
equipment performance
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chamber.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

CB (E6.1) 07/2017 Page 7/145
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
Stability hazard
Lifting hazard
Gas cylinders
Suffocation hazard
CO2suffocation and
poisoning hazard
Explosive substances
Fire promoting agents
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard

CB (E6.1) 07/2017 Page 8/145
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Service label
Hot surface
•on the outer chamber door
Risk of injury
•on the outer door: CB-UL only
•above the access ports (option)

CB (E6.1) 07/2017 Page 9/145
CO2-Incubator CB CO2-Incubator CB-UL
Figure 1: Position of labels on the chamber
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

CB (E6.1) 07/2017 Page 10/145
1.4 Type plate
Position of type plate: left chamber side (seen from front), at the bottom in the middle
Figure 2: Type plate (example of CB 160 regular chamber)
Indications of the type plate (example)
Information
BINDER
Manufacturer: BINDER GmbH
CB 160
Model designation
CO2 incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2017
Year of construction
Nominal
temperature
187 °C
369 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Class of temperature safety device
Art. No.
9040-0092
Art. No. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,30 kW
Nominal power
230 V / 50 Hz
Nominal voltage ±10% at the indicated power frequency
230 V / 60 Hz
1 N ~
Current type
5,7 A
Nominal current
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be disposed of
in separate collection according to Directive 2012/19/EU on
waste electrical and electronic equipment (WEEE).
(CB-UL only)
The chamber is certified by Underwriters Laboratories Inc.®
according to the following standards:
CAN/CSA-C22.2 No. 61010-1, 2nd Edition, 2004-07
UL 61010-1, 2nd Edition, 2005-07-22
Nominal temp.
187 °C
1,30 kW / 5,7 A
369 °F
230 V / 50 Hz
IP protection
20
230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9040-0092
Project No.
Built
2017
CO2 incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
CB 160
E6.1
Serial No. 00000000000000
Made in Germany

CB (E6.1) 07/2017 Page 11/145
1.5 General safety instructions on installing and operating the CO2incubator
With regard to operating the chamber and to the installation location, please observe the guideline
BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory
guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the chamber in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the chamber into operation.
DANGER
Electrical hazard.
Danger of death.
∅The chamber must NOT become wet during operation or maintenance.
The chambers were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).

CB (E6.1) 07/2017 Page 12/145
During and after a sterilization the temperature of the inner surfaces almost equals the set-point.
CAUTION
The inner doors, the inner door and glass door handles, and the inner chamber will
become hot during a sterilization.
Danger of burning.
∅Do NOT touch the inner doors, the inner door and glass door handles, and the inner
surfaces after a sterilization.
WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.
1.6 Precautions when working with gases
Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2warning system
WARNING
High concentration of CO2(> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO2.

CB (E6.1) 07/2017 Page 13/145
Chamber with O2control: Notes on handling oxygen (O2)
Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes
and hair. O2is heavier than air and may accumulate in low-lying areas.
WARNING
High concentration of O2(> 21 % O2).
Fire and explosion hazard through contact of combustible materials with O2.
Risk of burns and other injuries.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling O2.
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.
General information for safe handling of oxygen:
•Make sure training of personnel on hazards of oxygen enrichmen
t and necessary safety
measures.
•Make sure adequate labeling of all oxygen equipment and facilities.
•Make sure gas tightness of all gas connections by checking
them for leaks (e.g. with leak
spray or diluted soap solution).
•Close the main valve of the source of oxygen after work when not using the chamber.
•Never lubricate O2
equipment with oil or fat. Use only materials and spare parts which are
approved for use with oxygen.
•Regularly inspect fire extinguishers for proper condition.
•Set up emergency showers where oxygen enrichment is possible.
•
Strictest smoking ban and no ignition sources in areas where oxygen enrichment is
possible.
•Make sure good ventilation of
areas where oxygen enrichment is possible (location of the
chamber and/or O2cylinders.
•Persons who may have been in a possibly oxygen-
enriched atmosphere must keep away
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least
15
minutes.
•Always keep emergency routes free.
Chamber with O2control: Notes on handling nitrogen (N2)
Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and
therefore practically imperceptible. Any N2gas that may escape must be safely led out via good room
ventilation or a suitable connection to an exhaust system.
WARNING
High concentration of N2.
Risk of death by suffocation.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling N2.

CB (E6.1) 07/2017 Page 14/145
1.7 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
•Store and use gas cylinders only in well-ventilated locations.
•Open the gas cylinder valve slowly to avoid pressure surges.
•Secure gas cylinders during storage and use against falling (chaining).
•Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
•Always close the valve even with apparently empty cylinders; screw
on the cap when not in
use. Return gas cylinders with the valve closed.
•Do not open gas cylinders by force. Mark them when damaged.
•Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
•Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

CB (E6.1) 07/2017 Page 15/145
1.8 Intended use
Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6°F. The chamber permits setting defined pH conditions by common NaHCO3buffer systems
of commercial cell media by keeping an exact CO2atmosphere inside. The chambers guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
With the chamber with O2control, a variable oxygen atmosphere can additionally influence the growth of
the cells.
The chambers are suitable for exact conditioning of harmless materials. Any possible solvent any solvent
must not be explosive and flammable. Components of the charging material must NOT form an explosive
mixture with air. The operating temperature must lie below the flash point or below the sublimation point
of the charging material. Any component of the charging material must NOT be able to release toxic
gases.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT introduce any substance combu
stible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
Other applications are not approved.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 19.1) are part of the intended use.
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Due to the special demands of the Medical Device Directive (MDD), these chambers are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.
The charging material shall not contain any corrosive ingredients that may damage the
machine components made of stainless steel, aluminum, and copper. Such ingredients
include in particular acids and halides. Any corrosive damage caused by such ingredients is
excluded from liability by BINDER GmbH.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the
chamber into systems or by special environmental or operating conditions in the sense of EN 61010-
1:2010. For this, the intended use of the chamber and all its connections must be observed.

CB (E6.1) 07/2017 Page 16/145
2. Chamber description
The CO2incubators CB are equipped with a multifunctional microprocessor display controller for
temperature, CO2, and O2(chamber with O2control) levels and a digital display accurate to one-tenth of a
degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and therefore
easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical
applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door
are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high
temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic
surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and
will in no way impair the function or quality of the chamber.
The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB
160), resp. 8 (CB 220) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
The chamber’s heating system permits hot-air auto-sterilization at a setpoint of 187.5 °C / 369.5°F. Thus,
a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal surfaces, resulting in
sterilization of the entire inner chamber. Therefore, this procedure meets all international guidelines
regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European Pharmacopoeia.
Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is
maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.
A highly precise, drift-free CO2infrared measuring system in combination with the permanent mixture of
CO2gas through a special proprietary gas mixing head developed by BINDER allows precise and
constant CO2concentrations for long periods. This creates optimum growth conditions for cultures.
The CO2incubator is also available with O2control in addition to CO2control. There are two different
control ranges:
•Regular equipment: Hypoxic control range 0.2 to 20 vol. % O2. Only N2can be connected to reduce
O2concentration; it is not possible to connect O2gas bottles to increase O2concentration. Control in
the low O2range is very precise, in particular in the range below 1 vol. % O2.
•Alternative control range 10 to 95 vol. % O2(option no. 8012-1106). Although the high control range is
intended in particular for hyperoxic applications (> 21 vol. % O2), it is also suitable for slightly hypoxic
applications between 10 and 20 vol. % O2.
CO2and O2sensors
Fast reaction times, maximum accuracy and selectivity characterize the CO2measuring procedure of
the CB incubator series. The accuracy of the CO2 measuring system is based on a double-beam
infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a
reference value. Therefore, disturbance variables and aging phenomena in the measuring system are
almost completely eliminated, so that this measuring system, in contrast to other measuring
procedures, remains practically drift-free between calibrations and is entirely selective for CO2. The
sensor is built into the chamber and can be sterilized.
The O2sensor is a semiconductor gas sensor with ZrO2ceramic.
The accuracy of the indicated values of CO2and O2(chamber with O2control) depends on the
ambient air pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the
CO2measurement, the controller measures the ambient air pressure and automatically includes it in
the calculation.
The chambers are equipped with an Ethernet interface for computer communication, e.g. with the
communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options, see
chap. 22.5.

CB (E6.1) 07/2017 Page 17/145
Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F
CO2range: 0 vol.-% up to 20 vol.-%)
O2range (chamber with O2control): 0.2 vol.-% up to 20 vol.-% (hypoxic control range) or 10 Vol.-%
up to 95 Vol.-% (alternative control range)
2.1 Chamber overview
Instrument panel with microprocessor
controller T4.12 and USB interface
Door handle
Chamber door
Main power switch
Figure 3: CO2incubator CB (example: model CB 160)
2.2 Instrument panel
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface

CB (E6.1) 07/2017 Page 18/145
2.3 Inner chamber
Figure 5: CB 160 with O2control and options
(A)
(B)
(C)
(D)
(
D2)
(G)
(F)
(E)
(
H)
(I)
(
J)
(
K)
(
L)

CB (E6.1) 07/2017 Page 19/145
Figure 6: CB 160 / CB 220 with O2control and options
(A) Instrument panel with microprocessor controller T4.12, indicating temperature and CO2as well as
O2(chamber with O2control)”
(B) Connection socket for extra-low voltage supply (option, chap. 15.6)
(C) CO2sensor
(D) Gas mixing head CO2
(D2) Additional gas mixing head O2/ N2(chamber with O2control)
(E) Pt 100 temperature probe
(F) O2sensor (chamber with O2control)
(G) Internal socket 230V (max. 3 A) (option, chap. 15.4)
(H) Perforated shelves, made of stainless steel
(I) Measuring access port
(J) Inner glass doors
(K) Permadry™ water pans
(L) Lower housing cover
(B)
(C)
(D)
(D2)
(G)
(F)
(E)

CB (E6.1) 07/2017 Page 20/145
2.4 Control panel on the rear of the chamber
(1) (2) (3) (6) (4) (5)
(7) (8) (9) (10)
Figure 7: Rear control panel CB with O2control and options
(1) Miniature fuse
(2) External socket for extra-low voltage supply (option for CB 160 / CB 220, chap. 15.6)
(3) DIN socket for analog outputs 4-20 mA (option, chap. 15.5)
(4) Quick acting closure socket for CO2
(5) Quick acting closure socket for O2(chamber with O2control and optional alternative control range
10 up to 95 vol.-% O2)
(6) Quick acting closure socket for N2(chamber with O2control)
(7) Socket for IEC connector plug for power cable
(8) Ethernet interface for computer communication
(9) DIN-socket for zero-voltage relay alarm outputs
(10) RS 422 interface for computer communication (option)