Binder CB Series User manual

Issue 01/2022 Art. No. 7001-0326
Operating Manual
CB / CB-UL, CBF / CBF-UL (E7)
CO2Incubators
CO2Incubators with O2Control
CO2Incubators with Humidity control
with sterilizable NDIR sensor system for CO2
and microprocessor display program controller MB2 with touchscreen
Model Model version Voltage Equipment Art. no.
CB 56
CB56-230V
200-230 V
9640-0005
CB 56
CB56-230V-O
200-230 V
O
2
control 0-20 vol.-%
9640-0006
CB 56-UL
CB56-120V
100-120 V
9640-0007
CB 56-UL
CB56-120V-O
100-120 V
O2control 0-20 vol.-%
9640-0008
CB 170
CB170-230V
200-230 V
9640-0009
CB 170
CB170-230V-O
200-230 V
O2control 0-20 vol.-%
9640-0010
CB 170-UL
CB170-120V
100-120 V
9640-0011
CB 170-UL
CB170-120V-O
100-120 V
O
2
control 0-20 vol.-%
9640-0012
CB 260
CB260-230V
200-230 V
9640-0013
CB 260
CB260-230V-O
200-230 V
O2control 0-20 vol.-%
9640-0014
CB 260-UL
CB260-120V
100-120 V
9640-0015
CB 260-UL
CB260-120V-O
100-120 V
O2control 0-20 vol.-%
9640-0016
Model Model version Voltage Equipment Art. no.
CBF 170
CBF170-230V
200-230 V
9640-0017
CBF 170
CBF170-230V-O
200-230 V
O2control 0-20 vol.-%
9640-0018
CBF 170-UL
CBF170-120V
100-120 V
9640-0019
CBF 170-UL
CBF170-120V-O
100-120 V
O2control 0-20 vol.-%
9640-0020
CBF 260
CBF260-230V
200-230 V
9640-0021
CBF 260
CBF260-230V-O
200-230 V
O2control 0-20 vol.-%
9640-0022
CBF 260-UL
CBF260-120V
100-120 V
9640-0023
CBF 260-UL
CBF260-120V-O
100-120 V
O2control 0-20 vol.-%
9640-0024
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Contents
1. SAFETY.................................................................................................................. 8
1.1 Personnel Qualification .......................................................................................................................8
1.2 Operating manual................................................................................................................................8
1.3 Legal considerations ...........................................................................................................................8
1.3.1 Intellectual property...................................................................................................................9
1.4 Structure of the safety instructions......................................................................................................9
1.4.1 Signal word panel .....................................................................................................................9
1.4.2 Safety alert symbol .................................................................................................................10
1.4.3 Pictograms ..............................................................................................................................10
1.4.4 Word message panel structure...............................................................................................11
1.5 Localization / position of safety labels on the chamber ....................................................................11
1.6 Type plate..........................................................................................................................................12
1.7 General safety instructions on installing and operating the CO2incubator.......................................13
1.8 Precautions when working with gases ..............................................................................................15
1.9 Precautions when handling gas cylinders.........................................................................................17
1.10 Intended use .....................................................................................................................................18
1.11 Foreseeable Misuse..........................................................................................................................19
1.12 Residual Risks ..................................................................................................................................20
1.13 Operating instructions .......................................................................................................................21
1.14 Measures to prevent accidents .........................................................................................................22
1.15 CBF / CBF-UL : Resistance of the humidity sensor against harmful substances.............................23
2. CHAMBER DESCRIPTION .................................................................................. 24
2.1 Chamber overview ............................................................................................................................25
2.2 Inner chamber ...................................................................................................................................26
2.3 Control panel on the rear of the chamber .........................................................................................27
2.4 Instrument panel ...............................................................................................................................28
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 28
3.1 Unpacking, and checking equipment and completeness of delivery ................................................28
3.2 Guidelines for safe lifting and transportation.....................................................................................29
3.3 Storage..............................................................................................................................................29
3.4 Location of installation and ambient conditions ................................................................................30
4. INSTALLATION AND CONNECTIONS ............................................................... 33
4.1 Shelves..............................................................................................................................................33
4.2 Permadry™ water pan ......................................................................................................................34
4.3 Connecting the O2sensor (chamber with O2control) .......................................................................35
4.4 Gas connections ...............................................................................................................................35
4.4.1 Connection of the CO2gas cylinder........................................................................................36
4.4.2 Connection of the O2gas cylinder (chamber with O2control with optional alternative control
range 10 up to 95 vol.-% O2) ..................................................................................................37
4.4.3 Connection of the N2gas cylinder (chamber with O2control).................................................38
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2) ............................................39
4.4.5 Gas cylinder connection kits (option)......................................................................................41
4.5 CBF / CBF-UL: Water supply and dehumidification..........................................................................41
4.5.1 General remarks on water supply...........................................................................................41
4.5.1.1 Water supply....................................................................................................................41
4.5.1.2 Dehumidification ..............................................................................................................42
4.5.1.3 Types of suitable water quality ........................................................................................42
4.5.1.4 BINDER Pure Aqua Service (option)...............................................................................42
4.5.2 Water supply via freshwater bag (standard) ...........................................................................43
4.5.2.1 Scope of delivery .............................................................................................................43
4.5.2.2 Installation and connection of the freshwater bag...........................................................43

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4.5.2.3 Refilling the already installed freshwater bag..................................................................44
4.5.2.4 Replacing the tube connectors for freshwater supply .....................................................45
4.5.3 Water supply via prefilled water bottle (optional humidification water set) .............................46
4.5.3.1 Scope of delivery .............................................................................................................46
4.5.3.2 Installation, connection and change of the prefilled water bottle ....................................46
4.5.4 Water supply via freshwater can (optional water container set).............................................48
4.5.4.1 Scope of delivery .............................................................................................................49
4.5.4.2 Installation and connection of the freshwater can (optional water container set) ...........49
4.5.4.3 Refilling the freshwater can .............................................................................................51
4.6 Electrical connection .........................................................................................................................52
4.7 Divided inner door with cell therapy compartmentation (optional)....................................................53
4.7.1 Special model CBF 260 “Cell Therapy” ..................................................................................53
4.7.2 Handling and aligning the divided inner door .........................................................................54
4.7.3 Mounting the partition walls for cell therapy compartmentation .............................................55
5. FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER............... 57
5.1 Operating functions in normal display...............................................................................................58
5.2 Display views: Normal display, program display, chart-recorder display..........................................59
5.3 Controller icons overview ..................................................................................................................60
5.4 Operating modes...............................................................................................................................62
5.5 Controller menu structure..................................................................................................................63
5.5.1 Main menu ..............................................................................................................................64
5.5.2 “Settings” submenu.................................................................................................................65
5.5.3 “Service” submenu..................................................................................................................65
5.6 Principle of controller entries.............................................................................................................66
5.7 Performance during and after power failures....................................................................................66
5.8 Performance when opening the door................................................................................................67
6. START UP ............................................................................................................ 67
6.1 Turning on the chamber ....................................................................................................................67
6.2 Controller settings upon start up .......................................................................................................68
6.3 Factory settings.................................................................................................................................69
6.4 Equilibration time...............................................................................................................................69
6.5 Operating the DuoDoor™ door lock..................................................................................................70
6.6 Required gas supply for the chamber with O2control.......................................................................70
6.6.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O2(regular) ..............................................70
6.6.2 Alternative control range 10 vol.-% up to 95 vol.-% (available via BINDER INDIVIDUAL
Customized Solutions) ............................................................................................................70
6.6.3 Operation without O2control...................................................................................................71
6.7 Activating / deactivating CO2, O2and humidity control .....................................................................71
6.8 CB / CB-UL: Humidity control of the Permadry™ system.................................................................72
7. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE ........................... 73
7.1 Set-point entry through the “Setpoints” menu...................................................................................74
7.2 Direct setpoint entry via Normal display............................................................................................75
7.3 Special controller functions via operation lines .................................................................................76
7.4 Safety instructions for setting high gas concentrations.....................................................................77
8. TIMER PROGRAM: STOPWATCH FUNCTION .................................................. 78
8.1 Starting a timer program ...................................................................................................................78
8.1.1 Performance during program delay time ................................................................................78
8.2 Stopping a running timer program ....................................................................................................79
8.2.1 Pausing a running timer program ...........................................................................................79
8.2.2 Cancelling a running timer program........................................................................................79
8.3 Performance after the end of the program........................................................................................79
9. TIME PROGRAMS ............................................................................................... 80
9.1 Starting an existing time program .....................................................................................................80
9.1.1 Performance during program delay time ................................................................................81

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9.2 Stopping a running time program......................................................................................................81
9.2.1 Pausing a running time program.............................................................................................81
9.2.2 Cancelling a running time program.........................................................................................81
9.3 Performance after the end of the program........................................................................................81
9.4 Creating a new time program............................................................................................................82
9.5 Program editor: program management.............................................................................................82
9.5.1 Deleting a time program..........................................................................................................83
9.6 Section editor: section management.................................................................................................84
9.6.1 Add a new program section ....................................................................................................85
9.6.2 Copy and insert or replace a program section........................................................................85
9.6.3 Deleting a program section.....................................................................................................86
9.7 Value entry for a program section.....................................................................................................87
9.7.1 Section duration ......................................................................................................................87
9.7.2 Set-point ramp and set-point step...........................................................................................88
9.7.3 Special controller functions via operation lines.......................................................................90
9.7.4 Setpoint entry..........................................................................................................................91
9.7.5 Tolerance range......................................................................................................................91
9.7.6 Repeating one or several sections within a time program......................................................92
9.7.7 Saving the time program.........................................................................................................93
10. WEEK PROGRAMS ............................................................................................. 93
10.1 Starting an existing week program....................................................................................................93
10.2 Cancelling a running week program .................................................................................................94
10.3 Creating a new week program ..........................................................................................................94
10.4 Program editor: program management.............................................................................................95
10.4.1 Deleting a week program ........................................................................................................96
10.5 Section editor: section management.................................................................................................97
10.5.1 Add a new program section ....................................................................................................98
10.5.2 Copy and insert or replace a program section........................................................................98
10.5.3 Deleting a program section.....................................................................................................99
10.6 Value entry for a program section.....................................................................................................99
10.6.1 Set-point ramp and set-point step modes...............................................................................99
10.6.2 Weekday ...............................................................................................................................100
10.6.3 Start time...............................................................................................................................100
10.6.4 Setpoint entry........................................................................................................................101
10.6.5 Special controller functions via operation lines.....................................................................101
11. NOTIFICATION AND ALARM FUNCTIONS ...................................................... 102
11.1 Notification and alarm messages overview.....................................................................................102
11.1.1 Notifications ..........................................................................................................................102
11.1.2 Alarm messages ...................................................................................................................103
11.2 State of alarm..................................................................................................................................105
11.3 Resetting an alarm, list of active alarms .........................................................................................105
11.4 Activating / deactivating the audible alarm (alarm buzzer) .............................................................106
11.5 Tolerance range settings and alarm delay times ............................................................................106
11.6 Zero-voltage relay alarm output ......................................................................................................108
12. TEMPERATURE SAFETY DEVICES................................................................. 109
12.1 Over temperature protective device (class 1) .................................................................................109
12.2 Overtemperature safety controller class 3.1 ...................................................................................109
12.2.1 Safety controller modes ........................................................................................................110
12.2.2 Setting the safety controller ..................................................................................................110
12.2.3 Message and measures in the state of alarm.......................................................................111
12.2.4 Function check......................................................................................................................111
13. USER MANAGEMENT....................................................................................... 112
13.1 Authorization levels and password protection.................................................................................112
13.2 Log in...............................................................................................................................................115
13.3 Log out ............................................................................................................................................116

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13.4 User change....................................................................................................................................116
13.5 Password assignment and password change.................................................................................117
13.5.1 Password change .................................................................................................................117
13.5.2 Deleting the password for an individual authorization level..................................................119
13.5.3 New password assignment for “service” or “admin” authorization level when the password
function was deactivated ......................................................................................................120
13.6 Activation code................................................................................................................................121
14. GENERAL CONTROLLER SETTINGS.............................................................. 122
14.1 Selecting the controller’s menu language .......................................................................................122
14.2 Setting date and time ......................................................................................................................122
14.3 Selecting the temperature unit ........................................................................................................124
14.4 Display configuration.......................................................................................................................124
14.4.1 Adapting the display parameters ..........................................................................................124
14.4.2 Touchscreen calibration........................................................................................................125
14.5 Network and communication...........................................................................................................126
14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions) ..........126
14.5.2 Ethernet ................................................................................................................................127
14.5.2.1 Configuration .................................................................................................................127
14.5.2.2 Display of MAC address................................................................................................128
14.5.3 Web server............................................................................................................................128
14.5.4 E-Mail....................................................................................................................................129
14.6 USB menu: Data transfer via USB interface ...................................................................................130
15. GENERAL INFORMATION ................................................................................ 131
15.1 Service contact page.......................................................................................................................131
15.2 Current operating parameters.........................................................................................................131
15.3 Event list..........................................................................................................................................132
15.4 Technical chamber information.......................................................................................................132
16. CHART RECORDER DISPLAY ......................................................................... 133
16.1 Views...............................................................................................................................................133
16.1.1 Show and hide legend ..........................................................................................................133
16.1.2 Switch between legend pages ..............................................................................................133
16.1.3 Show and hide specific indications .......................................................................................134
16.1.4 History display.......................................................................................................................134
16.2 Setting the parameters....................................................................................................................137
17. CBF / CBF-UL: HUMIDIFICATION SYSTEM..................................................... 138
17.1 Function of the humidifying system.................................................................................................138
17.2 Function of the dehumidifying system.............................................................................................139
18. OPTIONS............................................................................................................ 139
18.1 APT-COM™ 4 Multi Management Software (option)......................................................................139
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)..........139
18.3 Quick sample access (option).........................................................................................................140
18.4 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions).............141
18.5 Analog outputs for temperature and CO2(option) ..........................................................................142
18.6 Data Logger kits (option).................................................................................................................143
18.7 Access port for extra-low voltage (option).......................................................................................143
18.8 BINDER Gas Supply Service – External bottle changer for CO2, N2or O2(option) .......................144
18.9 Stands .............................................................................................................................................145
18.9.1 Stacking stand (option) .........................................................................................................145
18.9.2 Base on castors (option).......................................................................................................145
18.10 Water supply sets (optional for CBF / CBF-UL) ..............................................................................145
18.10.1 Tubular bag set (option)........................................................................................................145
18.10.2 Humidification water set (option) ..........................................................................................145
18.10.3 Water container set (option) .................................................................................................145

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19. REFERENCE MEASUREMENTS ...................................................................... 146
19.1 CO2reference measuring ...............................................................................................................146
19.1.1 Measuring CO2concentration indirectly via the pH of the cell medium................................146
19.1.2 Measuring CO2directly via chemical indicator tubes............................................................147
19.1.3 Measuring CO2directly with an electronic infrared measuring device .................................147
19.2 Temperature reference measurement ............................................................................................147
20. AVOIDING MICROBIAL CONTAMINATION...................................................... 148
20.1 Cells and media ..............................................................................................................................148
20.2 Laboratory conditions / equipment around the incubator................................................................148
20.3 Working and behavior in the lab .....................................................................................................148
20.4 Chamber design and equipment of the CO2incubator ...................................................................149
20.5 Handling the CO2incubator ............................................................................................................150
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .. 151
21.1 Cleaning ..........................................................................................................................................151
21.2 Decontamination / chemical disinfection of the chamber................................................................153
21.2.1 Disinfection of the CO2sensor..............................................................................................154
21.2.2 CBF / CBF-UL: Disinfection of components of the fresh water supply system ....................154
21.3 Hot-air sterilization ..........................................................................................................................155
21.3.1 Overview ...............................................................................................................................155
21.3.2 Preparation for a hot-air sterilization.....................................................................................156
21.3.3 Starting the sterilization cycle and running the hot-air sterilization.......................................156
21.3.4 Prematurely terminating the sterilization cycle .....................................................................158
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization ....................................................................................................................
158
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization ................................................................159
21.3.5 Completing the entire sterilization cycle ...............................................................................160
22. MAINTENANCE AND SERVICE, TROUBLESHOOTING, REPAIR, TESTING. 161
22.1 General information, personnel qualification...................................................................................161
22.2 CBF / CBF-UL: Replacing components of the humidifying / dehumidifying system.......................162
22.2.1 Replacing the silicone tube of the fresh water supply system..............................................162
22.2.2 Replacing the pump head of the fresh water supply system ................................................162
22.2.3 Replacing the gas fine filter (sterile filter) for ambient air .....................................................162
22.3 Replacing the CO2und O2/N2gas inlet fine filters (sterile filters)....................................................163
22.4 Replacing the CO2sensor...............................................................................................................164
22.5 Maintenance intervals, service........................................................................................................165
22.6 Simple troubleshooting....................................................................................................................166
22.6.1 General .................................................................................................................................166
22.6.2 Heating..................................................................................................................................167
22.6.3 Gas cylinder pressure too low ..............................................................................................168
22.6.4 Gas control............................................................................................................................169
22.6.5 Sterilization ...........................................................................................................................171
22.6.6 Humidity (CB / CB-UL)..........................................................................................................171
22.6.7 Humidity (CBF / CBF-UL) .....................................................................................................172
22.6.8 Controller ..............................................................................................................................172
22.6.9 Open door .............................................................................................................................173
22.7 Sending the chamber back to BINDER GmbH ...............................................................................173
23. DISPOSAL.......................................................................................................... 174
23.1 Disposal of the transport packing....................................................................................................174
23.1.1 Outer chamber packing.........................................................................................................174
23.1.2 Packing inside the chamber and equipment.........................................................................174
23.2 Decommissioning............................................................................................................................175
23.3 Disposal of the chamber in the Federal Republic of Germany .......................................................175

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23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................176
23.5 Disposal of the chamber in non-member states of the EU .............................................................178
24. TECHNICAL DESCRIPTION.............................................................................. 178
24.1 Factory calibration and adjustment .................................................................................................178
24.2 Over current protection ...................................................................................................................178
24.3 Definition of usable volume.............................................................................................................179
24.4 Technical data.................................................................................................................................179
24.5 Equipment and Options (extract) ....................................................................................................182
24.6 Accessories and spare parts (extract) ............................................................................................183
24.7 Important conversion data for non-SI units .....................................................................................186
24.8 Conversion table for gas inlet pressures, bar – psi.........................................................................186
24.9 Dimensions......................................................................................................................................187
24.9.1 Chamber size 56...................................................................................................................187
24.9.2 Chamber size 170.................................................................................................................188
24.9.3 Chamber size 260.................................................................................................................189
25. CERTIFICATES AND DECLARATIONS OF CONFORMITY............................. 190
25.1 EU Declaration of conformity for CB ...............................................................................................190
25.2 EU Declaration of conformity for CBF.............................................................................................192
26. CONTAMINATION CLEARANCE CERTIFICATE ............................................. 194
26.1 For chambers located outside the USA and Canada .....................................................................194
26.2 For chambers in the USA and Canada ...........................................................................................197

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Dear customer,
For the correct operation of the CO2incubator, it is important that you read this operating manual completely
and carefully and observe all instructions as indicated. Failure to read, understand and follow the
instructions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment
performance
1. Safety
1.1 Personnel Qualification
The chamber must only be installed, tested, and started up by personnel qualified for assembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education, knowledge,
experience and knowledge of relevant standards allow them to assess, carry out, and identify any potential
hazards in the work assigned to them. They must have been trained and instructed, and be authorized, to
work on the chamber.
The chamber should only be operated by laboratory personnel especially trained for this purpose and
familiar with all precautionary measures required for working in a laboratory. Observe the national
regulations on minimum age of laboratory personnel.
1.2 Operating manual
This operating manual is part of the components of delivery. Always keep it handy for reference in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe the safety instructions of the operating manual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the instructions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
Carefully read the complete operating instructions of the chamber prior to installing and
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manual is supplemented and updated as needed. Always use the most recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-to-
dateness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe
installing, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the
contents and the product described are subject to change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use
and safety during operation and maintenance. Images are to provide basic understanding. They may
deviate from the actual version of the chamber. The actual scope of delivery can, due to optional or special
design, or due to recent technical changes, deviate from the information and illustrations in these
instructions this operating manual.

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In no event shall BINDER be held liable for any damages, direct or incidental arising out of or related to the
use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively
valid statement of warranty administration and the general terms and conditions, as well as the legal
regulations valid at the time the contract is concluded. The statements in this manual neither augment nor
restrict the contractual warranty provisions.
1.3.1 Intellectual property
Trademark Information: All BINDER trademarks relating to products or service, as well as trade names,
logos and product names used on the website, products and documents of BINDER company are
trademarks or registered trademarks of BINDER company (including BINDER GmbH, BINDER Inc.) in the
U.S. and other countries and communities of states. This includes word marks, position marks,
word/figurative marks, design configurations, figurative marks, and design patents.
Patent Information: BINDER products, categories of products, and accessories may be covered by one
or more patents and/or utility models in the U.S. and other countries and communities of states. Additional
patent applications may also be pending in the U.S. and other countries and communities of states.
Please visit www.binder-world.com for more information.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.

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1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.4.3 Pictograms
Warning signs
Electrical hazard
Hot surface Explosive atmosphere Stability hazard
Lifting hazard
Gas cylinders
Suffocation hazard
CO2suffocation and
poisoning hazard
Explosive substances
Fire promoting agents
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard
Magnetic field
Mandatory action signs
Mandatory regulation Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch
Do NOT spray with
water
Information to be observed in order to ensure optimum function of the product.

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1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.5 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Service label
Hot surface
•on the outer chamber door
Risk of injury
•on the outer door: CB-UL / CBF-UL only
•above the access ports (option)
Magnetic field
•on the outer door above the door handle
CO2incubator CB / CBF CO2incubator CB-UL / CBF-UL
Figure 1: Position of labels on the chamber

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CO2incubator CBF CO2incubator CBF-UL
Figure 2: Position of additional labels on the CBF / CBF-UL
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.
1.6 Type plate
Position of type plate: left chamber side (seen from front), at the bottom in the middle
Figure 3: Type plate (example of CB 170 regular chamber)
Indications of the type plate (example)
Indication
Information
BINDER
Manufacturer: BINDER GmbH
CB 170
Model designation
CO2 incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2022
Year of construction
Nominal
temperature
180 °C
356 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Class of temperature safety device
Art. No.
9640-0009
Art. No. of the chamber
Var.
9640-0025
Variant No. / Equipment
Project No.
---
Optional: Special application acc. to project no.
1,30 kW
Nominal power
200-230 V / 50 Hz
Nominal voltage range +/-10%
at the indicated power frequency
200-230 V / 60 Hz
1 N ~
Current type
5,7 A
Nominal current
Nominal temp.
180 °C
1,30 kW / 5,7 A
356 °F
200-230 V / 50 Hz
IP protection
20
200-230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9640-0009
Var. 9640-0000
Project No.
Built
2022
CO2 incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
CB 170
E7
Serial No. 00000000000000
Made in Germany

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Symbols on the type plate
Symbol
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on the market in the
EC after 13 August 2005 and to be disposed of in separate collection according
to Directive 2012/19/EU on waste electrical and electronic equipment (WEEE).
The chamber is certified according to Customs Union Technical Regulation (CU
TR) for the Eurasian Economic Union (Russia, Belarus, Armenia, Kazakhstan
Kyrgyzstan).
1.7 General safety instructions on installing and operating the CO2incubator
With regard to operating the chamber and to the installation location, please observe the local and national
regulations relevant for your country (for Germany: DGUV guidelines 213-850 on safe working in
laboratories, issued by the employers’ liability insurance association).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap. 3.4).
Do not install or operate the chamber in hazardous locations.
DANGER
Danger of explosion due to combustible dusts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.

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Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy and humidity (CBF / CBF-
UL).
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products which may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the chamber into operation.
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅The chamber must NOT become wet during operation, cleaning, or maintenance.
∅Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a splash-proof manner.
The chambers were produced in accordance with VDE regulations and were routinely tested in accordance
to VDE 0411-1 (IEC 61010-1).
During and after a sterilization the temperature of the inner surfaces almost equals the set-point. The inner
doors, the inner door and glass door handles, and the inner chamber will become hot during a sterilization.
CAUTION
Danger of burning by touching hot chamber parts during or after a sterilization.
Burns.
∅Do NOT touch the inner surfaces, inner doors, inner door handles, and door gaskets
during or after a sterilization.
WARNING
Danger of injury and damages by the chamber tipping over.
Injuries and damage to the chamber and the charging material
∅Do NOT load the chamber door with heavy objects while it is open.

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The chamber is equipped with a permanent magnet located behind the inner panel of the outer chamber
door. If persons with active implants (e.g. pacemakers, defibrillators) keep a sufficient safe distance
(distance of field source to implant), an influence of these implants can be excluded with high probability.
WARNING
Magnets can affect the function of pacemakers and implanted defibrillators.
A pacemaker can be put into test mode and cause discomfort. A defibrillator may
not work anymore.
If you wear such devices, maintain a sufficient safety distance to the chamber door in the
area of the door handle.
Warn wearers of such devices of approaching the chamber door.
1.8 Precautions when working with gases
Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless and
therefore practically imperceptible. Vent out any CO2gas that may escape via good room ventilation or a
suitable connection to an exhaust system. We recommend installing a CO2warning system.
DANGER
Danger of suffocation and poisoning by high concentration of CO2(> 4 Vol.-%).
Death by suffocation.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO2.
Close the CO2supply when decommissioning the chamber.
Chamber with O2control: Notes on handling oxygen (O2)
Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes and
hair. O2is heavier than air and may accumulate in low-lying areas.
DANGER
Danger of fire and explosion through contact of combustible materials with O2
with high concentration of O2(> 21 % O2).
Serious injury or death from burns and / or explosion pressure.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling O2.
Close the O2supply when decommissioning the chamber.

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Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.
General information for safe handling of oxygen:
•Make sure training of personnel on hazards of oxygen enrichmen
t and necessary safety
measures.
•Make sure adequate labeling of all oxygen equipment and facilities.
•Make
sure gas tightness of all gas connections by checking them for leaks (e.g. with leak
spray or diluted soap solution).
•Close the main valve of the source of oxygen after work when not using the chamber.
•Never lubricate O2
equipment with oil or fat. Use only materials and spare parts which are
approved for use with oxygen.
•Regularly inspect fire extinguishers for proper condition.
•Set up emergency showers where oxygen enrichment is possible.
•Strictest smoking ban and no ignition sources in areas where oxygen enrichment is possible.
•
Make sure good ventilation of areas where oxygen enrichment is possible (location of the
chamber and/or O2cylinders.
•Persons who may have been in a possibly oxygen-enriched atmosphere must keep away
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15 minutes.
•Always keep emergency routes free.
Chamber with O2control: Notes on handling nitrogen (N2)
Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and therefore
practically imperceptible. Any N2gas that may escape must be safely led out via good room ventilation or
a suitable connection to an exhaust system.
DANGER
Risk of suffocation through high concentration of N2.
Death by suffocation.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling N2.
Close the N2supply when decommissioning the chamber.

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1.9 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
•Store and use gas cylinders only in well-ventilated locations.
•Open the gas cylinder valve slowly to avoid pressure surges.
•Secure gas cylinders during storage and use against falling (chaining).
•Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
•Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
•Do not open gas cylinders by force. Mark them when damaged.
•Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
•Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Risk of injury through sudden release of the stored pressure energy when the valve
safety is torn off.
Injuries.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Risk of injury through sudden release of the stored pressure energy when opening
the cylinder valve of a not connected cylinder.
Injuries.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

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1.10 Intended use
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 22.5) are part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
CB / CB-UL and CBF / CBF-UL incubators are suitable for the cultivation of mammal cells under typical
conditions of approx. 37 °C / 98.6°F. The chamber permits setting defined pH conditions by common
NaHCO3buffer systems of commercial cell media by keeping an exact CO2atmosphere inside. The
chambers guarantee high humidity inside to avoid osmolarity increasing caused by the evaporation of the
cell media.
With the chamber with O2control, a variable oxygen atmosphere can additionally influence the growth of
the cells.
The chambers are suitable for exact conditioning of harmless materials.
Requirements for the chamber load
Any solvent must not be explosive and flammable. Components of the charging material must NOT form
an explosive mixture with air. The operating temperature must lie below the flash point or below the
sublimation point of the charging material. Any component of the charging material must NOT be able to
release toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the machine
components made of stainless steel, aluminum, and copper. Such ingredients include in particular acids
and halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
Contamination of the chamber by toxic, infectious or radioactive substances must be prevented
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material

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In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber
into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010. For this,
the intended use of the chamber and all its connections must be observed.
Medical devices
The chambers are not classified as medical devices as defined by Regulation (EU) No 2017/745.
Due to the special demands of the Medical Products legislation, these chambers are not
qualified to perform sterilization of medical devices as defined by Regulation (EU) No
2017/745.
Personnel Requirements
Only trained personnel with knowledge of the Operating Manual can set up and install the chamber, start it
up, operate, clean, and take it out of operation. Service and repairs call for further technical requirements
(e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements described in the Operating Manual for installation site and ambient conditions (Chap.
3.4) must be met.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if
this is feasible.
Relevant regulations for dealing with CO2/ O2/ N2and gas cylinders must be observed.
1.11 Foreseeable Misuse
Other applications than those described in chap. 1.10 are not approved.
This expressly includes the following misuses (the list is not exhaustive), which pose risks despite the
inherently safe construction and existing technical safety equipment:
•Non-observance of Operating Manual
•Non-observance of information and warnings on the chamber (e.g. control unit messages, safety
identifiers, warning signals)
•Installation, startup, operation, maintenance and repair by untrained, insufficiently qualified, or
unauthorized personnel
•Missed or delayed maintenance and testing
•Non-observance of traces of wear and tear
•Insertion of materials excluded or not permitted by this Operating Manual.
•Non-compliance with the admissible parameters for processing the respective material.
•Failure to comply with the relevant regulations for handling gas cylinders
•Failure to comply with the relevant regulations for handling CO2, O2or N2
•Operation of the chamber without ventilation measures
•Installation, testing, service or repair in the presence of solvents
•Installation of replacement parts and use of accessories and operating resources not specified and
authorized by the manufacturer
•Installation, startup, operation, maintenance or repair of the chamber in absence of operating
instructions

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•Bypassing or changing protective systems, operation of the chamber without the designated protective
systems
•Non-observance of messages regarding cleaning and disinfection of the chamber.
•Spilling water or cleaning agent on the chamber, water penetrating into the chamber during operation,
cleaning or maintenance.
•Cleaning activity while chamber is turned on.
•Operation of the chamber with a damaged housing or damaged power cord.
•Continued operation of the chamber during an obvious malfunction
•Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
•Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and other risks from incorrect operation, it is recommended the operator issue operating
instructions and standard operating procedures (SOPs).
1.12 Residual Risks
The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks,
even during correct operation. These residual risks include hazards which, despite the inherently safe
design, existing technical protective equipment, safety precautions and supplementary protective
measures, cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of these
residual risks and the measures required to prevent them are listed in the Operating Manual. Moreover, the
operator must take measures to minimize hazards from unavoidable residual risks. This includes, in
particular, issuing operating instructions.
The following list summarizes the hazards against which this Operating Manual and the Service Manual
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installation
•Sliding or tilting the chamber
•Setup of the chamber in unauthorized areas
•Installation of a damaged chamber
•Installation of a chamber with damaged power cord
•Inappropriate site of installation
•Missing protective conductor connection
Normal operation
•Assembly errors
•Contact with hot surfaces on the housing
•Contact with hot surfaces in the interior and inside of doors
•Emission of non-ionizing radiation from electrical operating resources
•Contact with live parts in normal state
Cleaning and Decontamination
•Penetration of water into the chamber
•Inappropriate cleaning and decontamination agents
•Enclosure of persons in the interior