WPI Eagle E2 User manual

INTRODUCTION
Seven new and improved color-coded
Eagle Pipetters cover a volume range
from 0.2 µL to 5,000 µL. These Eagle
Pipetters are a third-generation WPI
product incorporating a novel seal
(Models E20 through E5000) comprised
of a material at least five times more
durable than is currently available in other
brands. The Eagle pipetters are made of
high quality materials that are UV radiation
and solvent resistant, autoclavable (shaft and
tip ejector arm) and scratch resistant. The
continuously adjustable volume function is
designed to provide precision and accuracy
via a self-locking micro-counting mechanism.
Eagle Pipetters are easily user-calibrated. The
redesigned digital indicator permits easy reading
for either right- or left-handed use. The new
Eagle series also incorporates an improved
ergonomic design. A tip ejection button is
provided for the quick, easy and safe removal of
disposable tips (all Models except E5000).
Eagle™Adjustable Pipetters
World Precision Instruments
www.wpiinc.com
Fig. 1 — Pipetter Stand #14239, included free with
custom sets of five or more pipetters, holds seven
pipetters.
4. If the volume is different from that on the Digital Volume
Indicator, using the M4 hex wrench provided, loosen the three
set screws on the calibration wheel. (Caution: the Calibration
Wheel is the upper half of the Volume Adjustment Wheel; do
not loosen set screws on the lower locking wheel.) Turn the
adjustment ring (Fig. 5) clockwise to reduce the volume and
counter-clockwise to increase the volume.
5. Repeat Steps 1, 2, 3 and 4 until the volume measured is
within the percentage of accuracy expected as shown in the
Specifications. If this cannot be achieved, the pipetter should be
serviced by a qualified calibration service.
6. Tighten the three set screws on the calibration wheel after calibration.
SPECIFICATIONS
Model Volume Range Volume Accuracy Repeatability
E2 0.2-2mL 0.2mL ±12% (±0.024mL) ±6.0% (±0.012mL)
1.0mL ±2.7% (±0.027mL) ±1.3% (±0.013mL)
2mL ±1.5% (±0.03mL) ±0.7% (±0.014mL)
E10 0.5-10mL 1mL ±2.5% (±0.025mL) ±1.3% (±0.0125mL)
5mL ±1.5% (±0.075mL) ±0.6% (±0.03mL)
10mL ±1.0% (±0.1mL) ±0.4% (±0.04mL)
E20 2-20mL 2mL ±7.5% (±0.15mL) ±2.0% (±0.04mL)
5mL ±5.0% (±0.25mL) ±1.5% (±0.075mL)
10mL ±3.0% (±0.3mL) ±1.0% (±0.1mL)
20mL ±3.0% (±0.6mL) ±0.8% (±0.16mL)
E100 20-100mL 20mL ±3.0% (±0.6mL) ±1.0% (±0.02mL)
50mL ±2.0% (±1mL) ±0.5% (±0.25mL)
100mL ±1.5% (±1.5mL) ±0.3% (±0.3mL)
E200 50-200mL 50mL ±3.0% (±1.5mL) ±1.0% (±0.5mL)
100mL ±1.3% (±1.3mL) ±0.7% (±0.7mL)
200mL ±1.3% (±2.6mL) ±0.5% (±1mL)
E1000 100-1000mL 100mL ±2.0% (±2mL) ±0.8% (±0.8mL)
500mL ±1.0% (±5mL) ±0.4% (±2mL)
1000mL ±1.0% (±10mL) ±0.3% (±2.8mL)
E5000 1000-5000mL 1000mL ±1.3% (±13mL) ±0.4% (±4mL)
2500mL ±0.8% (±22.5mL) ±0.3% (±7.5mL)
5000mL ±0.8% (±40mL) ±0.2% (±10mL)
World Precision Instruments, Inc.
USA: International Trade Center, 175 Sarasota Center Boulevard, Sarasota FL 34240-9258 USA
Tel: [phone] • Fax: [phone] • E-mail: [e-mail] • Internet: http://www.wpiinc.com
ACCESSORIES
E2-OS Replacement O-Ring, Seal
E10-OS Replacement O-Ring, Seal
E20-OS Replacement O-Ring, Seal
E100-OS Replacement O-Ring, Seal
E200-OS Replacement O-Ring, Seal
E1000-OS Replacement O-Ring, Seal
E5000-OS Replacement O-Ring, Seal
500801 Filter for model E5000 (Bag of 100)
14239 Eagle Pipetter Stand
WPI Universal Pipetter Tips are ultra-clear and certified
RNase/DNase-free, available in sterile racks and non-sterile
racks and bulk packs. Contact WPI for prices.
Fig. 5 — Calibration
adjustment ring, located
under push button.
031608
Warranty
WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment,
including its components and parts, shall be free from defects in material and workmanship for a
period of one year* from the date of receipt. WPI’s obligation under this warranty shall be limited
to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon
receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A. Return of a repaired instrument shall be f.o.b.
Sarasota.
The above warranty is contingent upon normal usage and does not cover products which
have been modied without WPI’s approval or which have been subjected to unusual physical or
electrical stress or on which the original identication marks have been removed or altered. The
above warranty will not apply if adjustment, repair or parts replacement is required because of
accident, neglect, misuse, failure of electric power, air conditioning, humidity control, or causes
other than normal and ordinary usage.
To the extent that any of its equipment is furnished by a manufacturer other than WPI, the
foregoing warranty shall be applicable only to the extent of the warranty furnished by such other
manufacturer. This warranty will not apply to appearance terms, such as knobs, handles, dials or
the like.
WPI makes no warranty of any kind, express or implied or statutory, including without
limitation any warranties of merchantability and/or tness for a particular purpose. WPI shall
not be liable for any damages, whether direct, indirect, special or consequential arising from a
failure of this product to operate in the manner desired by the user. WPI shall not be liable for any
damage to data or property that may be caused directly or indirectly by use of this product.
Claims and Returns
• Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed loss or damage should be reported at
once to the carrier and an inspection requested. All claims for shortage or damage must be
made within 10 days after receipt of shipment. Claims for lost shipments must be made within
30 days of invoice or other notication of shipment. Please save damaged or pilfered cartons
until claim settles. In some instances, photographic documentation may be required. Some
items are time sensitive; WPI assumes no extended warranty or any liability for use beyond the
date specied on the container.
• WPI cannot be held responsible for items damaged in shipment en route to us. Please enclose
merchandise in its original shipping container to avoid damage from handling. We recommend
that you insure merchandise when shipping. The customer is responsible for paying shipping
expenses including adequate insurance on all items returned.
• Do not return any goods to WPI without obtaining prior approval and instructions (RMA#) from
our returns department. Goods returned unauthorized or by collect freight may be refused. The
RMA# must be clearly displayed on the outside of the box, or the package will not be accepted.
Please contact the RMA department for a request form.
• Goods returned for repair must be reasonably clean and free of hazardous materials.
• A handling fee is charged for goods returned for exchange or credit. This fee may add up to
25% of the sale price depending on the condition of the item. Goods ordered in error are also
subject to the handling fee.
• Equipment which was built as a special order cannot be returned.
• Always refer to the RMA# when contacting WPI to obtain a status of your returned item.
• For any other issues regarding a claim or return, please contact the RMA department
Warning: This equipment is not designed or intended for use on humans.

5. Depress the Push Button slightly,
moving it from the Initial Position to the First
Stop, as shown in Fig. 3. Hold the pipetter
vertically and immerse the tip to a depth of
2-4 mm for 2 to 3 seconds in the solution to
be pipetted. Release the Push Button slowly
and allow it to return to the Initial Position.
The solution is drawn up after a brief interval.
The pipetter should then be removed from the
solution and maintained at a vertical angle
until the liquid is dispensed.
6. Place the tip close to the inner wall of the receiving vessel and depress the Push Button
to the First Stop. Continue to depress the Push Button and progress to the Second
Stop. At the same time, move the tip along the inner wall of the vessel in order to fully
discharge the solution. Finally, release the button completely and remove the pipetter
from the vessel.
7. Depress the tip ejector button to release the tip into an appropriate waste container.
MAINTENANCE AND STORAGE
Pipetters are accurate and precise instruments and should be handled appropriately. Care
needs to be taken to prevent dropping the pipetter. WPI offers a stand for convenient and
safe storage. The pipetter may be wiped down with a cleaning agent such as 75% ethanol,
10% bleach or mild soap.
It is recommended that all non-routine maintenance, including replacement of internal
pipetter parts, be performed by a qualified technician or calibration service.
Pipetter Calibration
The pipetter is precalibrated with distilled water before shipment. However, recalibration
is needed from time to time. The frequency and use of the pipetter will determine the time
between calibrations. Whenever components are replaced, calibration is recommended,
with the exception of the filter for model E5000 which can be changed without requiring
recalibration.
CAUTION: To reduce the errors due to surface characteristics of the tip, the pipette tip
should first be wetted by aspirating and dispensing the water several times. To reduce
error from the evaporation of water, prior to performing the calibration procedure,
add some water to a container and place it in the analytical balance chamber in order
to saturate the air. In calibrating volumes below 50 µL, pipette the liquid into a capillary tube
instead of a container to further reduce the evaporation.
The user can perform calibration as follows:
1. Bring all equipment and distilled water to room temperature.
2. Adjust the dial to the desired volume (see Specifications). Pipette the distilled water into
a container positioned on an analytical
balance.
3. Weigh the water. Calculate the pipetted
volume in µL by dividing the
weight in mg by the density of
the distilled water at a given
temperature. Correct as needed
for any additional factors.
Eagle™Adjustable Pipetters
Description
Eagle Pipetters are variable volume, continuously adjustable
pipetters for the accurate and precise delivery of laboratory
fluids. The desired volume is obtained by turning the
Volume Adjustment Wheel until the correct volume appears
in microliters on the Digital Volume Indicator. The Volume
Adjustment Wheel also incorporates the Calibration Wheel
that is accessed only while performing calibration service
(see below). The stainless steel tip ejector may be removed
by simply pulling it down and out from the handle. The
color-coordinated Shaft Collar, when unscrewed, permits
the disassembly of the unit into the handle and shaft
components. The shaft and tip ejector arm can then be
autoclaved, if desired.
OPERATION
CAUTION: To prevent liquid from flowing into the internal cavity of the pipetter,
never place a pipetter upside down or lay it down horizontally if it is fitted with a
fluid-filled pipette tip.
Autoclaving
The shaft and tip ejector arm may be disassembled from the pipetter and are autoclavable at
120° C (15 PSI/1 bar) for 15 minutes. Recalibration is typically not required.
Filter Barrier
Eagle Pipetter E5000 incorporates a replaceable filter barrier to help prevent fluid from
reaching the piston should accidental aspiration into the shaft occur. If the filter becomes wet
or contaminated, remove it and insert a replacement, gently pushing it up until it stops.
Instructions for Use
1. No set-up is required after unpacking. The Eagle Pipetter is ready to use without
adjustment or calibration. (M4 hex wrench provided for recalibration when necessary.)
2. Choose the appropriate pipetter to use ensuring that the desired volume is within the
variable volume range of the pipetter.
3. Fix the disposable pipette tip to the pipetter. Be certain that the tip is fitted securely to
the pipette shaft. To ensure specified accuracy and precision, use WPI Universal Pipetter
Tips.
4. Turn the Volume Adjustment Wheel until the Digital Volume Indicator displays the
desired volume. See table below for volume ranges, examples of volume settings and
increments for each pipetter.
Push Button
Shaft Col-
lar
Digital
Volume
Indicator
Tip Ejector
(not on E5000)
Volume Adjust-
ment Wheel
Tip Ejector
Arm (not
on E5000)
Dispos-
able Tip
Fig. 2
Fig. 3
INITIAL POSITION
FIRST STOP
SECOND STOP
MODEL E2 E10 E20 E100 E200 E1000 E5000
Volume/Color 2 µL
Pink
10 µL
Light Blue
20 µL
Yellow
100 µL
Royal
Blue
200 µL
Lemon-
Lime
1000 µL
Ruby
5000 µL
Emerald
Volume Range 0.2-2 µL 0.5-10 µL 2-20 µL 20-100 µL 50-200 µL 100-1000 µL 1000-5000 µL
100% Volume
Setting 2.00 µL 10.0 µL 20.0 µL 100 µL 200 µL 1.00 mL 5.00 mL
Reading
10% Volume
Setting 0.20 µL 1.00 µL 2.00 µL 10.0 µL 20.0 µL 0.10 mL 0.50 mL
Reading
Smallest
Increment 0.002 µL 0.02 µL 0.02 µL 0.2 µL 0.2 µL 2 µL 2 µL
For accurate dispensing, use each pipetter for volumes within its stated volume range.
To ensure specified accuracy and precision, use WPI
Universal Pipetter Tips.
Handle
Shaft
Calibration
Wheel
Locking Wheel
(DO NOT ATTEMPT TO ADJUST)
Fig. 4
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