Olympus U-AFA2M-VIS User manual

U-AFA2M
ACTIVE AUTOFOCUSING UNIT
A X 8 3 2 0
This instruction manual is for the Olympus Active AF Unit Model U-AFA2M. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this unit, we recommend
that you study this manual thoroughly before operating the unit.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
U-AFA2M-VIS/DUV
U-AFA2M-CB
U-AFA2M-CBL2M/CBL3M
Modules described in this manual
INSTRUCTIONS
Optical Microscope Accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.
For Korea only
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This product is applied with the requirements of standard IEC/EN61326-1 concerning
electromagnetic compatibility.
- Emission Class A, applied to industrial environment requirements.
- Immunity Applied to industrial environment requirements.
Some interference may occur if this product is used in domestic location.

U-AFA2M
CONTENTS
5
6
7-10
10
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 MICROSCOPE MODULES
2 NOMENCLATURE
3
4 SYSTEM STARTUP PROCEDURE
5
6 TV OBSERVATION/PHOTOMICROGRAPHY
7 SPECIFICATIONS
8 TROUBLESHOOTING GUIDE
1- 4
11, 12
13
14 ,15
16,17
ASSEMBLY
— Have your local Olympus representative perform the assembly operations. —
REFLECTED LIGHT DIC AND SIMPLIFIED POLARIZED LIGHT
OBSERVATIONS
1Applicability of the System 2Notes on AF (Autofocusing)
3Getting Ready 4Maintenance and Storage
5Caution

1
U-AFA2M
IMPORTANT
· The U-AFA2M active AF unit is an intermediate attachment to be used with the BX61 microscope or the
BXFMA-F motorized vertical illuminator. The AF (autofocusing) operation is available by running the
dedicated software on a PC.
· This unit employs a laser diode (wavelength 775-800 nm) as the AF light source. The pupil division
active AF system enables high-speed focusing and the multi-spot projection improves the stability of
focusing of specimens with variable difference in heights.
· The following two kinds of AF units are available.
U-AFA2M-VIS: Visible AF unit (For exclusive use with visible light).
U-AFA2M-DUV: DUV AF unit (For visible and DUV light).
“Caution --- Use of controls or adjustment or performance of procedures other than those specified herein may
result in hazardous radiation exposure.”
1. This unit uses a laser diode (wavelength 775-800 nm) for autofocusing. It is designed to be safe by reducing the laser
power (Class 1), but it is still not recommended to view the laser light directly by removing the cover, etc. (Before mainte-
nance, be sure to set the power switch to “ ” (OFF).)
Never remove the warning and caution labels on the unit.
Class 1 Laser Product (IEC60825-1:2007 / IEC60825-1:2014)
This product complies with 21 CFR 1040.10 and 1040.11 except for deviations
pursuant to Laser Notice No. 50, dated June 24, 2007.
2. When using the DUV AF unit in combination, keep in mind that DUV (Deep Ultra-Violet light) is harmful to your eyes and
skin. Be sure to observe strictly the cautions given in the instruction manual provided with the DUV microscopy unit.
3. Always use the power cord provided by Olympus. If the proper power cord is not used, the product performance as well
as its safety performance cannot be warranted.
4. Always ensure that the grounding terminal is properly grounded/earthed. If the equipment is not grounded, Olympus
can no longer warrant the electrical safety performance of the equipment.
5. Distribute the connection cables at a distance from the lamp housing. If a connection cable contacts the lamp housing
or its surroundings, the cable may be melted and cause an electric shock hazard.
6. Never insert metallic objects into the air vents of the unit as this could result in electrical shock, personal injury and
equipment damage.
7. To prevent the microscope system from turning over, do not install modules which cause the microscope system height
to exceed 1 meter.
8. Do not place anything on the top of the AF unit as it may fall.
Safety Precautions
Safety Symbols
The following symbols are found on the unit. Study the meaning of the symbols and always use the equipment in the
safest possible manner.
Symbol Explanation
Before use, carefully read the instruction manual. Improper use could result in personal injury
to the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.

2
Warning labels
Warning labels related to laser beam are attached to the unit. Always heed the warnings.
Warning label position (U-AFA2M AF unit)
Left side panel Right side panel
The border and letter are black
and the background is yellow.
When a warning label becomes dirty or is peeled off, contact Olympus for replacement.
CAUTION Use of controls or adjustment or performance of procedures other than those specified herein may
result in hazardous radiation exposure.
1Applicability of the System
1. Applicable modules
· Motorized microscope frame: BX61TRF
· Motorized vertical illuminator: BX-RLAA or BXFMA-F
· Motorized revolving nosepieces
For brightfield objectives: U-D6REMC, U-P5REMC or U-D6REM
For brightfield/darkfield objectives: U-D6BDREMC, U-D5BDREMC U-P5BDREMC, or U-D5BDREM
(Note) When a brightfield objective is mounted on a revolving nosepiece by means of the BD-M-AD objective
adapter, the AF performance is not guaranteed due to deviation of centering of focusing.
· Objectives
Brightfield objectives: LMPLFLN 5X to 100X, MPLFLN 5X to 100X, LMPlanFl 5X to 100X, UMPlanFl 5X to
100X, LMPlanApo 150X or MApo 100X for 248 nm light (exclusively for use in DUV
observation)
Brightfield/darkfield objectives: LMPLFLN 5X to 100XBD, MPLFLN 5X to 150XBD, LMPlanFl 5X to 100XBD, UMPlanFl
5X/10X/20XBD (BD2) and 50X/100XBD, LMPlanApo 150XBD, MPlanFl 50XBD (BD2)
or MPplanFl 100XBD (BD2).
(Note) Objectives with WDs (Working Distances) of 1 mm or more are recommended. When the WD is below 1 mm,
special care is required in setting the AF zone and Near limit.
· Observation tube: U-BI30-2, U-TR30-2, U-ETR-4, U-TLU, U-SW TR-3, U-SWETR, U-SWETTR-5 or MX-
SWETTR.
}The previous objective series are also applicable provided that the model names are the same.
2. Applicable observation methods
}For details on the observation methods, read the instruction manuals for the microscope and microscope accessories.
· Reflected light brightfield, reflected light darkfield, reflected light DIC (Differential Interference Contrast)*, reflected light
simplified polarized light*and reflected light DUV (Deep Ultra-Violet light) ** observations
* The MX-AFDIC analyzer slot unit, U-AN analyzer and U-PO-3 polarizer are required.
** The U-AFA2M AF unit and the U-UVF248 DUV microscopy unit are required.
· The FN (Field Number) of the eyepieces should be no more than 22. The FN of the camera/TV light path is no more
than 20.
· In the DIC observation, AF may sometimes be impossible due to reduction of return light in certain retardation positions.
· In the DIC and simplified polarized light observations, the visual field may become irregular with certain specimens.
· Except in the DIC observation, be always sure to use a dummy slider to prevent flare.
· In the CCD camera observation, the laser spot for AF may be visible when the CCD camera does not incorporate an IR
cut filter (this does not cause a safety problem).

3
U-AFA2M
1. Applicable specimens
AF is available with any specimen with reflectivity from 3% (glass) to 100% (mirror).
However, the AF position may be deviated from the desired focusing position in the following cases.
· When observing a specimen with double structure (deposited thin film or an electrode under glass, etc.): AF on the
desired layer may be unavailable due to the multi-layer reflections.
· When observing a thin specimen with reflection from backside (glass, etc.): The AF may be unstable due to the backside
reflection.
· When observing a specimen with a regular fine pattern using a low-power objective.
· When observing a specimen with diffused surface reflections using a low-power objective.
· When observing a specimen in the reflected light DIC observation: AF may be unavailable in certain retardation positions.
Stopping the Stage in the Middle of Motorized Focusing Operation
2Notes on AF (Autofocusing)
1. This unit is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. Do not use the unit where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.(For the
operating conditions, refer to chapter 7, “SPECIFICATIONS” on page 15.)
Fig. 1
@
The movement of the stage can be stopped immediately by turning the
fine adjustment dial @ on the BX61 microscope frame (in either direction).
When the unit is mounted on the BXFMA, the same effect can be obtained
using the focusing knob of the U-FH.
Recovering from an Error in the Motorized Focusing Module
}When the focusing module catches something in the middle of motorized
operation, a “focusing module error” occurs and the focusing operation
is stopped.
Recovery procedure
1. Provided that the motor operation error is not occurring, the caught item
can be taken out by turning the fine adjustment dial @.
2. If the motor operation error also occurred, the fine adjustment dial is
defeated. In this case, remove the nearby modules and take out the
caught item. Be sure to attach the removed nearby modules after this.
3. Set the main switch to “ ” (OFF) then “ ” (ON) again.
The microscope will restart unless the motor is failed.
3Getting Ready

4
1. Do not attempt to use organic solvents to clean the unit and the modules but wipe them dry with a soft cloth. When they
are extremely dirty, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
2. To clean a glass component of the analyzer or polarizer, etc., simply blow dirt away using a commercially available blower
and wipe gently using a piece of cleaning paper (or clean gauze).
If a glass component is stained with fingerprints or oil smudges, wipe it with cleaning paper slightly moistened with
commercially available absolute alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
3. Do not disassemble any part of the unit as this could result in malfunction or reduced performance.
4. When not using the microscope system, make sure that the lamp housing is cool enough, and cover the microscope
system with the dust cover before storage.
5. When disposing of this unit, check the regulations and rules of your local government and be sure to observe them.
If the unit is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
4Maintenance and Storage
5Caution
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).

5
U-AFA2M
* The modules marked *are provided to the U-AFA2M active AF unit.
Other required applicable modules are to be prepared separately.
1
MICROSCOPE MODULES
Observation Tube
U-BI30-2
U-TR30-2
U-ETR-4
U-TLU
U-SWTR-3
U-SWETR
U-SWETTR-5
MX-SWETTR
Motorized Vertical Illuminator
BX-RLAA
BAFMA-F
Motorized Revolving
Nosepiece
U-D6REMC
U-P5REMC
U-D6REM
U-D6BDREMC
U-D5BDREMC
U-P5BDREMC
U-D5BDREM
Motorized Microscope Frame
BX61TRF
DIC Slider
U-DICR
U-DICRH
U-DICRHC
Analyzer Slot/Analyzer
MX-AFDIC/U-AN
Active AF Unit*
U-AFA2M-VIS
U-AFA2M-DUV
(Used in combination
with the U-UVF248)
Objectives
See page 2 for details on
the applicable objectives.
Control Box**
BX-UCB
Hand Switch
U-HSTR2
** The U-AFA2M active AF unit is provided with the U-AFA2M-CB AF control board, which is to be installed inside the BX-
UCB control box.

6
Active AF Unit U-AFA2M-VIS/U-AFA2M-DUV
Be sure to use the connection cable designated by Olympus.
The computer in use should meet the IEC60950 requirements.
If any item other than specified is used, the entire performance of the system cannot be guaranteed.
NOMENCLATURE
Observation tube mount
(Centering adjustment type)
Laser beam output
directions
Warning label (P. 2)
AF board connector
Warning label (P. 2)
AF Cable U-AFA2M-CBL2M (2 m)/U-AFA2M-CBL3M (3 m)
Connection cable
U-AFA2M BX-UCB (AF Control Board)
AF Control Board U-AFA2M-CB
}To be installed in the BX-UCB Control Box.
Termination connector
#If disconnected, AF operation
will be unavailable.
AF control board connector
The three other connectors
are not used.
CAUTION If another Z-focusing control board (U-
ZPCB, U-AFP1-CB, etc.) is already installed
in the BX-UCB, be sure to remove it before
installing this board.
Otherwise, malfunction will result.
Laser beam path A
Laser beam path B
Class 1 IEC60825-1:2007 / IEC60825-1:2014
Laser beam pathA:Below 1μW
Laser beam pathB:200μW Maximum

7
U-AFA2M
ASSEMBLY
3-1 Assembly Diagram
Brightfield/darkfield
objectives
(See page 2 for
details on the
applicable
objectives.)
# For the assembly and adjustments of the unit, have Olympus perform them.
# After re-assembling the module, re-adjustments are required. Consult Olympus in such a case.
}For the connection using an RS-232C cable (to be prepared by the user) and computer, refer to the instruction manuals
for the BX-UCB control box and U-HSTR2 hand switch.
CAUTION The BX-RLAA motorized vertical illuminator will not work properly if the connector of the U-UCD8A
motorized universal condenser is connected to the BX-UCB control box. Be sure to detach the
connector before using the illuminator.
}In consideration of the observation of metallurgical specimens, it is recommended to use a dedicated metallurgical stage
or replace the specimen holder with a stage plate to improve the ease of operation.
If only the stage plate is replaced, the back surface of the stage plate may be worn after long period of use because the
upper surface of the biological stage is coated with ceramic.
Brightfield objectives
(See page 2 for details on
the applicable objectives.)
Rotary wafer holder
BH2-WHR43 Stage plate
U-MSSP4*
Wafer holder
U-WHP2
Glass plate
U-MSSPG*
Stage plate
U-MSSP*
Stage
clamping
knob
4 x 4 inch stage
U-SIC4R2/SIC4L2
Stage
U-SVRM/
SVLM
Stage
clamping
screw
Observation tube
U-BI30-2 U-SWTR-3
U-TR30-2 U-SWETR
U-ETR-4 U-SWET TR-5
U-TLU MX-SWETTR
Analyzer slot unit**
MX-AFDIC
Analyzer
U-AN
Active AF unit
U-AFA2M-VIS/U-AFA2M-DUV
AF cable
U-AFA2M-
CBL2M/3M
Motorized vertical
illuminator
BX-RLAA
BXFMA-F
Control box
BX-UCB
AF control board
U-AFA2M-CB
Hand switch
U-HSTR2
Motorized microscope frame
BX61TRF
Motorized revolving
nosepiece
For brightfield objectives
For brightfield/darkfield
objectives
* Warp prevention pins (2 for each) are attached on the bottom sides of the U-MSSP/MSSP4 stage plate and the U-
MSSPG glass plate when the system is shipped from the factory. Remove the pins before use.
** Attach the MX-AFDIC so that the model nameplate faces straight the rear.
CAUTION When a brightfield objected is mounted on the revolving nosepiece by means of the BD-M-AD objective
adapter. the AF centering error may occur. In this case, the AF performance of the system cannot be
guaranteed.

8
3-2 Detailed Assembly Procedures
1Installing the U-AFA2M Active AF Unit (Figs. 2 & 3)
Fig. 2
@
²
Fig. 3
5
³
|
CAUTION Be sure to adjust centering of the AF unit.
To ensure safety, be sure to unplug the AF cable during installation
and adjustment of the AF unit.
1. Fully loosen the observation tube clamping screw @ using an Allen
screwdriver, attach the circular dovetail on the bottom of the active AF
unit ², and then tighten the clamping screw @ again.
#The centering of the AF unit should be adjusted before mounting it
on the observation tube.
2. Attach the standard straight tube ³ on the observation tube mount on
the AF unit, then insert the scaled CT | into the standard straight tube.
Rotate the helicoid on the top of the CT to focus on the cross scale.
3. Engage the objective with the highest magnification of the ones being
used in the light path.
4. Set the observation path of the vertical illuminator to reflected light
brightfield (BF).
5. Slide the top of the CT to focus on the objective pupil.
6. Loosen the clamping screws in the four holes 5on the top of the
sensor unit, align the center of the objective pupil with the cross of the
CT (within one scale in both up/down and left/right directions), then
tighten the clamping screws firmly.
7. Remove the standard straight tube and CT, and block the 4 holes
with the provided contamination stickers.

9
U-AFA2M
}Install only the U-AFA2M-CB AF control board in the BX-UCB control box. Remove any other Z-focusing control board
(U-ZPCB, U-AFP1-CB,etc.) if any is installed. Otherwise, malfunction will result.
#Do not unplug the termination connector. Otherwise, AF operation will be unavailable.
#The AF control board is a precision part that may be damaged critically by static electricity. Since the human
body is charged with a very small amount of static electricity, it should be discharged before handling the
board. The static electricity can be discharged by simply touching a nearby metallic object.
Changing the Settings of On-Board DIP Switches
2Installing the U-AFA2M-CB Control Board (Figs. 4 & 5)
}The on-board DIP switches on the AF control board have been set for
use with the BX61 before shipment from the factory.
In this condition, all switches of SW1, SW2 and SW3 are set to OFF.
When the AF Unit is Installed on the BX61
1. The DIP switches can be used without modifying the factory shipment
condition.
2. Change the DIP switch positions only when the following functions are
required.
SW2 Set No. 4 to ON.
· This replaces the functions of the F/C switching and stage escape
buttons on the left side of the BX61 with the stage DOWN and UP
functions.
SW3 Set No. 4 to ON.
· When the U-FWT filter wheel is not used, this changes the focus
adjustment stroke from 15 mm to 25 mm.
· Set the switch to OFF when the U-LWUCD long-WD universal
condenser is used.
When the AF Unit is Installed on the BXFMA
1. Be sure to change the settings of the DIP switches for the BXFMA.
SW2 Set No. 2 and No. 3 to ON.
SW3 Set No. 2 and No. 5 to ON*.
* This is required to align the focusing knob rotation directions with the
objective up/down movement directions when the U-FH is used. As
a result of this, rotating the focusing knob towards you move the
objective downward.
Fig. 4

10
Installing the AF Control Board
Mount all of the applicable modules selected by the user.
At the end, confirm that the main switches of the connected modules are
set to “ ” (OFF) and connect the cables.
}The system can be started using dedicated software (analySIS FIVE or MX2-BSW).
For details, refer to the instruction manual for the software.
#Be sure to set the main switch on the BX-UCB control box @ to “ ”
(OFF) before proceeding to installing the U-AFA2M-CB AF control
board.
1. Loosen the six clamping knobs on the two option slot covers on the rear
of the BX-UCB and remove the covers and knobs.
}To stabilize the installation operation, attach two of the removed clamping
knobs on the AF control board ².
2. Insert the AF control board ² into the BX-UCB by aligning the orientation
of the connector inside the BX-UCB and that of the AF control board.
Push in the board along the board rails all the way so that the connector
is plugged in securely.
}A space of two board rails is required for installation. Use two adjacent
option slots on either the central or right.
3. Clamp the AF control board ² using the clamping knobs removed above.
Also attach the other removed cover ³ using the other clamping knobs
removed above.
}Store the other, unused option board cover in a safe place.
Fig. 5
@
²
³
SYSTEM STARTUP PROCEDURE
3Assembling Other Modules

11
U-AFA2M
REFLECTED LIGHT DIC AND SIMPLIFIED
POLARIZED LIGHT OBSERVATIONS
(Note) The modules required for simplified polarized light observation are the MX-AFDIC analyzer slot, U-AN analyzer and U-
PO3 polarizer.
DIC (Differential Interference Contrast) observation is possible by adding the U-DICR series of DIC slider to the above
combinations.
# The performance of the polarizer may deteriorate when it has been exposed to light for a long period (about
continuous 2000 hours). If this happens, replace the polarizer.
#When using the high-intensity light source, be sure to use the U-25L42 UV cut filter for prevention of the
polarizer burn.
1. Insert the U-AN analyzer ² in the analyzer insertion slot of the analyzer
slot unit @ so that the analyzer’s surface with indication faces upward.
(The analyzer can also be inserted through the slot on the other side of
the unit.)
2. Insert the U-PO3 polarizer ³ so that the surface with indication comes
on the front.
}An approximate cross-Nicol position for the simplified polarized observation
can be obtained when both the analyzer and polarizer are engaged in
the light path. In this condition, polarized light observation can be started
immediately after placing a specimen.
1. Loosen the mounting knob @ on the front of the DIC revolving nosepiece,
insert the DIC slider ² so that the surface with indication faces up, and
clamp by tightening the mounting knob.
2. With the U-DICR interference slider, set the slide lever ³ according to the
objective in use.
1Setting the Analyzer and Polarizer (Fig. 6)
2Setting the DIC Slider (Fig. 7)
Fig. 6
@
²
³
Fig. 7
@
²
³
|
3. With the U-DICRH or U-DICRHC slider that does not have the slide lever,
the applicable objectives are as follows.
DIC Slider Applicable Objectives
U-DICRH MPLFLN/MPLFLN-BD series
UMPlanFl/UMPlanFl-BD series
MPlanFl-BD series
MPlanApo20X, 100X
U-DICRHC LMPLFLN/LMPLFLN-BD series
LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series
UIS2
UIS
UIS2
UIS
Lever ³ position Applicable Objectives
Pushed in UIS2 MPLFLN/MPLFLN-BD series
UIS UMPlanFl/UMPlanFl-BD series
MPlanApo20X, 100X
MPlanApo100XBD
Pulled out UIS2 LMPLFLN/LMPLFLN-BD series
UIS LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series

12
1. Place the specimen on the stage and adjust the focus by moving the
stage up or down.
2. Adjust the field iris diaphragm so that its image circumscribes the field of
view.
3. The contrast may sometimes be enhanced by closing the aperture iris
diaphragm slightly.
U-DICR U-DICRHC
1. Adjust the background contrast by turning the prism movement knob |
on the DIC slider as described below. (Fig. 7)
2. When the prism movement knob on the DIC slider is turned, the
interference color in the background varies continuously from the gray
sensitive color to magenta sensitive color (from -100 to 600 nm). Set the
interference color which can provide best contrast with respect to the
specimen.
· Selecting a gray background color enables 3D-looking observation with
high contrast thanks to the highest sensibility of the gray sensitive color.
· Selecting a magenta sensitive color allows even small phase variation to
be observed as a change in color.
U-DICRH
1. Adjust the background contrast by turning the prism movement knob |
on the DIC slider as described below. (Fig. 7)
2. When the prism movement knob on the U-DICRH DIC slider is turned,
the interference color in the background varies from -100 to 100 nm. Set
the interference color which can provide best contrast.
· Selecting a gray background color enables 3D-looking observation with
high contrast thanks to the highest sensibility of the gray sensitive color.
· Selecting a magenta sensitive color allows even small phase variation to
be observed as a change in color.
#Since the DIC observation has a high detection sensitivity, take care
against contamination on the specimen surface.
}The detection sensitivity is variable depending on direction so it is
recommended to use a rotary stage.
3Observation Method (Fig. 7)
1. Loosen the mounting knob @ on the front of the DIC revolving nosepiece,
gently pull out the DIC slider ² to remove, and insert the dummy slider
as far as it will go and then clamp it with the mounting knob.
2. Slide out the analyzer and polarizer to disengage them from the light
path.
4(Fig. 7)
Switching Between Brightfield and
Darkfield Observations

13
U-AFA2M
TV OBSERVATION/PHOTOMICROGRAPHY
}Use a trinocular observation tube or the U-TLU when observing microscope images on a TV screen or photographing
them.
The U-TV0.35XC-2/TV0.5XC-3/TV0.63XC C-mount camera adapter or the combination of U-TV1X-2 + U-CMAD3, etc.
can be mounted on the straight observation tube.
As adapters for mounting a camera with a mount other than the C-mount are also available, please contact Olympus.
1Selecting the TV Adapter Magnification
The magnification of the TV adapter is determined by the size of the CCD used in the TV camera or digital camera.
The following illustration shows the image pickup areas of the 0.5X TV adapter when only the U-AFA2M is used as the
intermediate attachment of the microscope.
1/3-inch CCD
2/3-inch CCD
1/2-inch CCD
Field of view of eyepieces (22 mm dia.)
Field of view of the camera (20 mm dia.)
CCD size C-mount camera adapter
*The four corners of image may be cut off or obscured.
}The fields of view of the eyepieces and camera vary depending on other intermediate attachments used.
U-TV0.35XC-2 U-TV0.5XC-3 U-TV0.63XC U-TV1X + U-CMAD3
1/3-inch 17.1 mm 12 mm 9.5 mm 6 mm
1/2-inch *22.9 mm 16 mm 12.7 mm 8 mm
2/3-inch *31.4 mm *22 mm 17.5 mm 11 mm
Intermediate attachments Eyepiece visual field Camera visual field
U-AFA2M 22 mm 20 mm
U-AFA2M + MX-AFDIC 22 mm 20 mm
U-AFA2M + U-UVF248 20.8 mm 18.6 mm
U-AFA2M + U-UVF248 + MX-AFDIC 20 mm 18.1 mm
When the 0.5X TV adapter is used

14
SPECIFICATIONS
AF Unit
Item U-AFA2M-VIS U-AFA2M-DUV
Applicable microscope frame BX61TRF + BX-RLAA
BXFMA
Applicable illuminator BX-RLAA, BXFMA-F
Applicable intermediate
attachment U-UVF248-IM
Applicable observation tube U-BI30-2, U-TR30-2, U-ETR-4, U-TLU, U-SWTR-3, U-SWETR, U-SWETTR-5, MX-SWETTR.
Applicable revolving nosepiece
U-D6REMC, U-D6BDREMC, U-D5BDREMC, U-P5REMC, U-P5BDREMC, U-D6REM, U-D5BDREM.
Applicable observation
modules
Polarizer: U-PO3, U-POTP3.
Analyzer: U-AN, U-AN360-3.
DIC slider: U-DICR, U-DICRH, U-DICRHC.
Analyzer slot unit: MX-AFDIC.
Applicable control box BX-UCB (The U-AFA2M-CB and dedicated software are required)
Controller Computer
AF detection method Pupil-division reflection active autofocusing using a laser diode and 2-division detector.
Multi-spot projection.
Laser wavelength: 775-800 nm (Class 1 IEC60825-1:2007 / IEC60825-1:2014)
Laser pulse duration: 0.5 to 2.5ms
Pulse frequency: 100 Hz.
Momentary maximum power: 200 μW. (Parallel beam)
Output from internal laser diode:
Beam divergence: // = 7 to 13 deg, = 23 to 33 deg
Maximum power: 10mW
Applicable objectives See page 2 for details on the applicable objectives.
Applicable observation
methods
Reflected light brightfield.
Reflected light darkfield.
Reflected light DIC (using U-AN analyzer +
MX-AFDIC).
Reflected light simplified polarized light (using
U-AN analyzer + MX-AFDIC).
Reflected light brightfield.
Reflected light darkfield.
Reflected light DIC (using U-AN analyzer +
MX-AFDIC).
Reflected light simplified polarized light (using
U-AN analyzer + MX-AFDIC).
Field number 22 (20 for camera observation)
AF rate 0.2 sec. from the proximity of focal point.
AF repeatability Within the focal depth of the objective in use.
Specimen tracking range 5X: ±5000 μm or more.
10X: ±2000 μm.
20X: ±1100 μm.
50X: ±400 μm.
100X: ±100 μm.
150X: ±50 μm.
The tracking range is dependent on the
specimen reflectivity and objective in use.
The figures above are some of examples in a
brightfield observation of an IC wafer
specimen (reflectivity about 45%) using a
LMPlanFl 5X to 100XBD or LMPlanApo150XBD
objective.
5X: ±5000 μm or more.
10X: ±2000 μm.
20X: ±1100 μm.
50X: ±400 μm.
100X: ±100 μm.
150X: ±50 μm.
DUV: ±50 μm.
The tracking range is dependent on the
specimen reflectivity and objective in use.
The figures above are some of examples in a
brightfield observation of an IC wafer
specimen (reflectivity about 45%) using a
LMPlanFl 5X to 100XBD or LMPlanApo150XBD
objective.
Dimensions 108(W) x 45(H) x 313(D) mm
Weight 2.6 kg

15
U-AFA2M
Operating Environment
· Indoor use.
· Altitude: Max. 2000 meters
· Ambient temperature:10 to 35°C (50 to 95° F)
· Relative humidity: 15% to 85%
· Supply voltage fluctuations: ±10%
· Pollution degree: 2 (in accordance with IEC60664)
· Installation (overvoltage) category: II (in accordance with IEC60664)

16
TROUBLESHOOTING GUIDE
Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems
occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking
the entire list, please contact Olympus for assistance.
Problem Cause Remedy Page
The unit cannot be turned ON. A wrong cable is connected. Connect the appropriate cable.
BX-UCB
Instructions
The focusing module is not initialized
after power ON.
The DIP switches are set improperly. Set them properly. 9
The AF control board is connected im-
properly.
Check its connection. 10
The control from the dedicated soft-
ware is not available.
The microscope is being initialized. Wait until the initialization completes
before launching the software.
Sof tware
Instructions
The DIP switches on the BX-UCB are
set improperly.
Set them properly.
BX-UCB
Instructions
A wrong RS-232C cable is connected. Connect a straight cable with D-SUB
9-pin female-female connectors.
BX-UCB
Instructions
There is wire connection or contact fail-
ure in the connection cables.
Check the connections of the cables. 7
AF is not successful in DIC observa-
tion.
The prism movement knob on the DIC
slider is set improperly.
Turn the prism movement knob and
retry AF operation.
AF is not successful.
No limits are set for the focusing mod-
ule.
An error occurs immediately after
the start of AF.
Set the focusing limits. Sof tware
Instructions
The AF zone is not set. Set the AF zone.
Sof tware
Instructions
The termination connector is unplugged. Plug it in. 6
A motorized unit is operating. Wait until the motorized unit completes
operation.
The AF laser spot is
captured in the
photomicrograph.
Use a CCD camera incorporating an
IR cut filter.
An error occurs after the focusing module starts to move after the start of AF.
The error occurs with all ob-
jectives.
The limits are set improperly. Re-set the focusing module limits.
Sof tware
Instructions
The AF zone is set improperly. Re-set the AF zone.
Sof tware
Instructions
The DIP switches are set improperly. Set them properly. 9
The retardation in DIC observation is
set to a position with low return light.
Execute AF with a different retardation
position, and then re-adjust it. 12
The objective on the revolving nose-
piece is different from that handled with
the control software.
Check if the objective on the revolving
nosepiece matches that handled with
the software.
Sof tware
Instructions
12
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