Binder C 170 User manual

Issue 08/2015 Art. No. 7001-0297
Operating Manual
C 170 (E3)
CO2Incubator
with FPI-sensor system
and display controller RP1
Model
Model version
Art. No.
C 170
C170-230V-R
9040-0112, 9140-0112
C 170-UL
C170-120V-R
9040-0113, 9140-0113
BINDER GmbH
Address Post office box 102
D-78502 Tuttlingen
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

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Contents
1. SAFETY.................................................................................................................. 5
1.1 Legal considerations ...........................................................................................................................5
1.2 Structure of the safety instructions......................................................................................................5
1.2.1 Signal word panel .....................................................................................................................5
1.2.2 Safety alert symbol ...................................................................................................................6
1.2.3 Pictograms ................................................................................................................................6
1.2.4 Word message panel structure.................................................................................................7
1.3 Localization / position of safety labels at the unit................................................................................7
1.4 Type plate ...........................................................................................................................................8
1.5 General safety instructions on installing and operating the CO2incubator ........................................9
1.6 Precautions when working with CO2gas ..........................................................................................11
1.7 Precautions when handling gas cylinders.........................................................................................11
1.8 Intended use .....................................................................................................................................12
2. UNIT DESCRIPTION ............................................................................................ 13
2.1 The CO2measuring principle ............................................................................................................13
2.2 Unit overview.....................................................................................................................................14
2.3 Connection panel at the rear of the unit............................................................................................16
2.4 Unit doors..........................................................................................................................................16
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION.................................................................................................... 17
3.1 Unpacking, and checking equipment and completeness of delivery ................................................17
3.2 Guidelines for safe lifting and transportation.....................................................................................18
3.3 Storage..............................................................................................................................................18
3.4 Location of installation and ambient conditions ................................................................................18
4. INSTALLATION AND CONNECTIONS ............................................................... 21
4.1 Shelf holder and shelves...................................................................................................................21
4.2 CO2sensor........................................................................................................................................22
4.2.1 Connecting the CO2sensor ....................................................................................................22
4.2.2 General notes .........................................................................................................................22
4.3 Water pan..........................................................................................................................................23
4.4 Gas connection .................................................................................................................................24
4.4.1 Connecting the CO2gas cylinder............................................................................................25
4.4.2 Connecting the gas hose to the unit rear................................................................................26
4.4.3 Gas cylinder connection kit (option)........................................................................................27
4.5 Electrical connection .........................................................................................................................28
5. START UP............................................................................................................ 29
6. FUNCTION OVERVIEW OF THE RP1 CONTROLLER ....................................... 29
6.1 Preset factory parameters.................................................................................................................30
6.2 Performance after turning on the unit ...............................................................................................30
7. CONTROLLER RP1 OPERATING MODES......................................................... 31
7.1 Selecting and setting the operating functions ...................................................................................32
7.2 Operating mode HAND .....................................................................................................................32
7.3 Operating mode USER: Advanced settings......................................................................................34
7.4 Operating mode LOCK: Locking/unlocking of the operating functions’ settings by operating mode
HAND ................................................................................................................................................38
7.5 Performance during and after power failures and shut down ...........................................................39
8. CONTROLLER RP1 SETTINGS .......................................................................... 39
8.1 Altitude of the installation site above sea level .................................................................................39
8.2 Entering the set points of temperature and CO2...............................................................................41

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9. TEMPERATURE SAFETY DEVICES................................................................... 42
9.1 Over temperature protective device (class 1) ...................................................................................42
9.2 Safety controller (temperature safety device class 3.1)....................................................................42
9.2.1 Setting the safety controller set point type..............................................................................43
9.2.2 Setting the safety controller set point......................................................................................44
10. ALARM FUNCTIONS ........................................................................................... 46
10.1 Alarm functions overview ..................................................................................................................46
10.2 Resetting the alarm messages .........................................................................................................46
10.3 Zero-voltage relay alarm output ........................................................................................................47
10.4 Safety controller temperature alarm..................................................................................................48
10.5 Temperature tolerance range alarm (high and low temperature) .....................................................48
10.6 Door open .........................................................................................................................................49
10.7 CO2tolerance range alarm (CO2over/under concentration) ............................................................50
10.8 CO2pressure too low ........................................................................................................................50
10.9 Temperature sensor failure...............................................................................................................51
10.10 Failure of CO2sensor........................................................................................................................52
11. ERROR MESSAGES............................................................................................ 52
12. REFERENCE MEASUREMENTS ........................................................................ 53
12.1 CO2reference measuring .................................................................................................................53
12.1.1 Measuring the CO2concentration indirectly via the pH of the cell medium............................53
12.1.2 Measuring CO2directly via chemical indicator tubes .............................................................54
12.1.3 Measuring CO2directly with an electronic infrared measuring device ...................................55
12.2 Temperature reference measurement ..............................................................................................55
13. OPTIONS.............................................................................................................. 55
13.1 Silicone access ports 30 mm / 1.18 in, closable with 2 silicone plugs (8012-0558 rear, 8012-0559
left, 8012-0560 right) (option)............................................................................................................55
13.2 Base on castors (option) ...................................................................................................................56
13.3 Stacking adapter for direct thermal decoupled stacking (option)......................................................56
13.1 Analog outputs for temperature and CO2(option) ............................................................................56
14. AVOIDING MICROBIAL CONTAMINATION........................................................ 57
14.1 Cells and media ................................................................................................................................57
14.2 Laboratory conditions / equipment around the incubator..................................................................57
14.3 Working and behavior in the lab .......................................................................................................57
14.4 Chamber design and equipment of the CO2incubator C 170 ..........................................................58
14.5 Handling the CO2incubator C 170....................................................................................................59
15. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION .... 60
15.1 Cleaning ............................................................................................................................................60
15.2 Decontamination / chemical disinfection of the CO2incubator .........................................................61
15.3 Disinfection of the CO2sensor..........................................................................................................63
15.4 Hot-air sterilization at 180 °C / 356 °F...............................................................................................64
15.4.1 Overview .................................................................................................................................64
15.4.2 Procedure for hot-air sterilization:...........................................................................................64
15.5 Aborting the hot-air sterilization ........................................................................................................67
15.5.1 Aborting the hot-air sterilization manually...............................................................................68
15.5.2 Aborting the hot-air sterilization by opening the outer door....................................................69
15.5.3 Aborting the hot-air sterilization using the main power switch................................................69
16. MAINTENANCE, AND SERVICE ......................................................................... 70
16.1 Maintenance intervals, service..........................................................................................................70
16.2 Checking the air jacket heating fan...................................................................................................70
16.3 Gas inlet fine filter .............................................................................................................................70
16.4 Sending the unit back to BINDER GmbH .........................................................................................71

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17. DISPOSAL ........................................................................................................... 72
17.1 Disposal of the transport packing......................................................................................................72
17.1.1 Outer unit packing...................................................................................................................72
17.1.2 Packing inside the unit, equipment.........................................................................................72
17.2 Decommissioning..............................................................................................................................73
17.3 Disposal of the unit in the Federal Republic of Germany .................................................................73
17.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany .74
17.5 Disposal of the unit in non-member states of the EC .......................................................................75
18. TROUBLESHOOTING ......................................................................................... 76
19. TECHNICAL DESCRIPTION................................................................................ 79
19.1 Factory calibration and adjustment ...................................................................................................79
19.2 Over current protection .....................................................................................................................79
19.3 Definition of usable volume...............................................................................................................79
19.4 C 170 technical data .........................................................................................................................80
19.5 Important conversion data for non-SI units.......................................................................................81
19.6 Conversion table for gas inlet pressures, bar – psi...........................................................................81
19.7 Equipment and options C 170...........................................................................................................82
19.8 Accessories and spare parts.............................................................................................................83
19.9 Dimensions C 170.............................................................................................................................84
20. CERTIFICATES.................................................................................................... 85
20.1 EC Declaration of Conformity............................................................................................................85
21. PRODUCT REGISTRATION ................................................................................ 87
22. CONTAMINATION CLEARANCE CERTIFICATE ............................................... 88
22.1 For units located outside North America and Central America.........................................................88
22.2 For units in North America and Central America ..............................................................................91

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Dear Customer,
For the correct operation of the CO2incubator C 170, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the unit and/or poor equipment
performance.
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injury and damage observe the safety instructions in the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and unit damage.
Observe the safety instructions in this operating manual
Carefully read the complete operating instructions for the CO2incubator C 170.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any dam-
ages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or exist-
ing agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on
the part of BINDER derive from the respective purchase contract, which also contains the entire and ex-
clusively valid statement of warranty administration. The statements in this manual neither augment nor
restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations in
accordance with the standards ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the seriousness and probability of serious consequences, potential dangers are identified
with a signal word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

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WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury
CAUTION
Indicates a potentially hazardous situation, which, if not avoided, may result in damage to the product
and/or its functions or to property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface
Explosive Atmosphere
Stability hazard
Lifting hazard
Gas cylinders
CO2suffocation and
poisoning hazard
Pollution Hazard
Harmful substances
Biohazard
Risk of corrosion and /
or chemical burns
Mandatory action signs
Mandatory regulation
Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles

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Prohibition signs
Do NOT touch
Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
∅Instruction on how to avoid the hazard: prohibition
Instruction on how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.
1.3 Localization / position of safety labels at the unit
The following labels are located on the unit:
Pictograms (Warning signs)
Service label
Hot surface
Risk of injury (UL units only).
Observe the safety instructions in the
operating manual.
Figure 1: Position of labels
on the CO2incubator C 170
Figure 2: Position of labels
on the CO2incubator C 170-UL

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Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.
1.4 Type plate
Figure 3: Position of type plate
Figure 4: Type plate C 170 (standard unit)
Indications of the type plate
(example)
Information
BINDER
Manufacturer: BINDER GmbH
C 170
Model designation
CO2 Incubator
Device name: CO2Incubator
Serial No.
00-00000
Serial No. of the unit
Built
2015
Year of construction
Nominal temperature
190 °C
374 °F
Nominal temperature
IP protection
20
Type of IP protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9040-0112
Art. No. of the unit
Project No.
---
Optional: Special application acc. to project no.
Nominal temp.
190 °C
1,10 kW / 6,1 A
374 °F
230 V / 50 Hz
IP protection
20
230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9040-0112
Project No.
Built
2015
CO2 Incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
C170
E3
Serial No. 00-00000
Made in Germany

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Indications of the type plate
(example)
Information
1,10 kW
Nominal power
6,1 A
Nominal current
230 V / 50 Hz
Nominal voltage ±10%
at the indicated power frequency
230 V / 60 Hz
1 N ~
Current type
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be disposed of
in a separate collection according to directive 2002/96/EC on
waste electrical and electronic equipment (WEEE).
(UL units only)
The equipment is certified by Underwriters Laboratories Inc.®
according to standards CAN/CSA-C22.2 No. 61010-1, 2nd Edi-
tion, 2004-07 (Electrical Equipment for Measurement, Control,
and Laboratory Use; Part 1: General Requirements); UL 61010-
1, 2nd Edition, 2005-07-22 (Electrical Equipment for Measure-
ment, Control, and Laboratory Use; Part 1: General Require-
ments); IEC 61010-1:2001, 2nd Edition and IEC 61010-2-10 (Par-
ticular Requirements for Laboratory Equipment for the heating of
materials).
1.5 General safety instructions on installing and operating the CO2incubator
With regard to operating the CO2incubator C 170 and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 la-
boratory guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or quali-
fied personnel authorized by BINDER perform all maintenance and repair, and if components relating to
chamber safety are replaced in the event of failure with original spare parts.
To operate the unit, use only original BINDER accessories or accessories from third-party suppliers au-
thorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the unit.
∅Do NOT install the unit in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the CO2incubator C 170 in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the unit in potentially explosive areas.
∅KEEP explosive dust or air-solvent mixtures AWAY from the unit.

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The CO2incubator C 170 does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the CO2incubator which is combustible or explo-
sive at working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy and humidity.
Familiarize yourself with any potential health risks caused by the charging material, the contained mois-
ture constituent or by reaction products that may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the CO2incubator into operation.
DANGER
Electrical hazard.
Danger of death.
∅The unit must NOT become wet during operation or maintenance.
The CO2incubators were produced in accordance with VDE regulations and were routinely tested in ac-
cordance to VDE 0411-1 (IEC 61010-1).
During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.
CAUTION
The glass door, the glass door handle and the inner chamber will become hot during
operation.
Danger of burning.
∅Do NOT touch the glass door, the glass door handle, the inner surfaces or the charging
material during operation.
WARNING
Stability hazard.
Danger of injury.
Damage to the unit and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the unit door is open.

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1.6 Precautions when working with CO2gas
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2warning system.
WARNING
High concentration of CO2(> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
∅Do NOT set up units in non-ventilated recesses
Ensure technical ventilation measures
Observe the relevant regulations for handling CO2.
1.7 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
•Store and use gas cylinders only in well-ventilated locations.
•Open the gas cylinder valve slowly to avoid pressure surges
•Secure gas cylinders during storage and use against falling (chaining).
•Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
•Always close the valve even with apparently empty c
ylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
•Do not open gas cylinders by force. Mark them when damaged
•Protect gas cylinders against fire, e.g. do not store together with flammable liquids
•Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

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1.8 Intended use
CO2incubators C 170 are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6 °F. The incubator permits setting defined pH conditions by common NaHCO3buffer systems
of commercial cell media by keeping an exact CO2atmosphere inside. C 170 incubators guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
Observing the instructions in this operating manual and conducting regular maintenance work
(chap. 16) is part of the intended use.
Other applications are not approved.
CO2incubators C 170 are not classified as medical devices as defined by the Medical Device Di-
rective 93/42/EEC.
Due to the special demands of the Medical Device Directive (MDD), these ovens are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.
WARNING: If customer should use a BINDER chamber running in non-supervised continu-
ous operation, we strongly recommend in case of inclusion of irrecoverable specimen or
samples to split such specimen or samples and store them in at least two chambers, if this is
feasible.
Always use proper protective equipment (clothing, gloves, safety glasses, etc.).
Always follow good hygienic practices according to GLP/SOP protocols.
Each individual operating the C 170 incubator is responsible for his or her own safety
Any component of the charging material must NOT be able to release toxic gases.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT introduce any substance combustible or explosive at working temperature into
the constant climate chamber.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
In case of foreseeable use of the device there is no risk for the user through the integration of the cham-
ber into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010.
For this, the intended use of the chamber and all its connections must be observed.

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2. Unit description
The CO2incubators C 170 are equipped with a microprocessor controller for temperature and CO2levels
and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are smooth and therefore
easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical
applications) and has no welds or inaccessible corners. The hinges and the seal of the inner glass door
are glued from the outside to aid cleaning of the inner chamber. When operating the chamber at high
temperatures (sterilization), the impact of the oxygen in the air may cause discoloration of the metallic
surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and
will in no way impair the function or quality of the unit.
The perforated shelves and the shelf holder are also made of stainless steel (German material no.
1.4016, US equivalent AISI 430). You can insert a maximum of 8 shelves. The shelf holder is demounta-
ble without any tool. In this way, you can clean it hygienically in every laboratory dishwasher before hot-
air sterilization.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
The heating system of the CO2incubator permits hot-air auto-sterilization at 190 °C / 374°F. Thus, a tem-
perature of at least 180 °C / 356°F is maintained on all internal surfaces, resulting in sterilization of the
entire inner chamber.
Thanks to the standard safety device (class 3.1 according to DIN 12880:2007), the set temperature is
maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.
A highly precise, drift-free CO2infrared measuring system in combination with the permanent mixture of
CO2gas through a special proprietary gas mixing head developed by BINDER allows precise and con-
stant CO2concentrations for long periods. This creates optimum growth conditions for cultures. The CO2
sensor can be removed from the inner chamber by hand and cleaned with suitable detergents if needed.
The CO2incubator can be operated in a temperature range from 7 °C / 12.6 °F above ambient tempera-
ture up to +50 °C / 122°F and a CO2range of 0 vol.-% up to 20 vol.-%.
2.1 The CO2measuring principle
Fast reaction times, as well as the highest accuracy and selectivity, characterize the CO2measuring pro-
cedure of the C 170 incubator series. The accuracy of the CO2 measuring system is based on a single-
beam infrared measuring cell, which measures in differential mode using the permanently alternating
transmission feature of its semi-conductor filter.
Due to this highly developed single-beam principle with Fabry-Perot interferometer (FPI), disturbance
variables and aging phenomena in the measuring system are almost completely eliminated, so that this
measuring system, in contrast to other measuring procedures, remains practically drift-free between cali-
brations and is absolutely selective for CO2.
The CO2measuring cell contains a measuring section inside, in which the absorption of infrared light
depends on the number of CO2molecules in the beam path. This number of CO2molecules changes with
the ambient pressure in relation to a constant volume. The distances between the molecules are conse-
quently pressure-dependent. The collision frequency of the IR-beam with CO2molecules increases there-
fore by increasing pressure. For this reason, the ambient pressure must be compensated in order to cor-
rect the display reading of the CO2concentration in vol.-%. This is achieved by entering the altitude of the
site above the sea (chap. 8.1).

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2.2 Unit overview
Figure 5: CO2incubator C 170, closed
(1) Triangle instrument panel with RP1 controller for temperature and CO2and
(2) Door handle
(3) Main power switch
(2)
(3)
(1)

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Figure 6: CO2incubator C 170, outer door open
(4) Glass door handle
(5) Water pan
(6) Shelves
(7) Shelf holder
(8) Pt 100 temperature sensor
(9) CO2sensor
(10) Gas mixing head for CO2
(11) Silicone measuring port in the glass door
(11)(10) (9) (8) (7)
(1)
(4) (5) (6)

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2.3 Connection panel at the rear of the unit
(12) (13) (14) (15) (16)
Figure 7: Rear connection panel C 170
(12) Miniature fuses
(13) Connection for IEC connector plug
(14) Ethernet interface for computer communication (option)
(15) Connection socket for zero-voltage relay alarm contact
(16) Quick acting closure socket for CO2gas cylinder
2.4 Unit doors
The outer door of the C 170 is equipped with a heater on its inner side. The door must be closed while the
unit is operating normally in order to ensure stable climatic conditions in the inner chamber.
An additional glass door enables viewing of the samples without disturbing the temperature in the interior
and contaminating the samples sealing the interior of the C 170.
When the outer door is open, the CO2intake valve automatically closes.
Delay time for the temperature and CO2tolerance range alarm :
After closing the outer door, the tolerance range alarm is turned off for a programmable delay
time. This prevents alarms being constantly triggered during the unstable operating phase
after opening the outer door.

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3. Completeness of delivery, transportation, storage, and installa-
tion
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt
for completeness and for transportation damage. Inform the carrier immediately if transportation damage
has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the unit.
Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
remove the operating manuals and accessory equipment.
For transport purpose, a silica gel bag for drying purpose was added. Do not eat! Do not open the silica
gel bag and dispose of it with normal waste.
Remove any protective lamination sheet on the inner metal surfaces prior to commissioning.
CAUTION
Sliding or tilting of the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
∅Do NOT lift or transport the unit using the door handle or the door.
Lift the unit from the pallet at the four lower corners with the aid of four people.
If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 17.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly
tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before commis-
sioning the unit.

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3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporarily decommissioning the unit (chap. 17.2).
Empty the water pan before moving the incubator. In case of any spilling of the contents, shut down the
incubator and dry it out carefully and completely
CAUTION
Sliding or tilting the unit.
Damage to the unit.
Risk of injury by lifting heavy loads.
Transport the unit in its original packaging only.
Secure the CO2incubator with transport straps for transport.
∅Do NOT lift or transport the unit using the door handle or the door.
Lift the unit at the four lower corners with the aid of 4 people and place it on a rolling
pallet.
Move the unit to the desired location and lift it from the rolling pallet with the aid of four
people.
•Permissible ambient temperature range for transport: -10 °C / 14°F to +60 °C / 140°F.
You can order transport packing and rolling pallets for transportation purposes from BINDER Service.
3.3 Storage
Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for tempo-
rary decommissioning (chap. 17.2).
•Permissible ambient temperature range for storage: -10 °C / 14°F to +60 °C / 140°F.
•Permissible ambient humidity: max. 70% r.H., non-condensing
When after storage in a cold location you transfer the unit to its warmer installation site, condensation
may form in the inner chamber, on the housing or in the sensor compartment of the CO2measurement.
Before start-up, wait at least one hour until the CO2incubator has attained ambient temperature and is
completely dry.
3.4 Location of installation and ambient conditions
Notes on the location of installation
Set up the CO2incubator on a flat, even surface, free from vibration and in a well-ventilated, dry location.
The chambers are designed for setting up inside a building (indoor use).
Freestanding C 170 incubators are suitable for installation on tables or on the optionally available stand.
Note: The site of installation must be capable of supporting the unit’s weight (see technical data, chap.
19.4).
Align the unit using a spirit level to ensure even covering of the cell-cultures with the medium. For this
purpose, manually adjust the four incubator feet.
In order to avoid contamination, never place the unit directly on the floor.

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CAUTION
Danger of overheating.
Damage to the unit.
∅Do NOT set up units in non-ventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
To completely separate the unit from the power supply, you must disconnect the power plug. Install the
unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.
For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010.
Do not install or operate the CO2incubator C 170 in potentially explosive areas.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the unit in potentially explosive areas.
∅KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.
Ambient conditions
•Permissible ambient temperature range for operation: +18 °C / 64.4 °F up to +30 °C / 86 °F. At elevat-
ed ambient temperature values, fluctuations in temperature can occur.
•Ideal ambient temperature: by at least 7 °C / 12.6 °F below the intended working temperature. E.g.,
working temperature 37 °C / 98.6 °F – resulting permitted ambient temperature 30 °C / 86°F and lower
In the event of working temperatures of less than 7 °C / 12.6 °F above the ambient temperature, the set
point can be exceeded.
The ambient temperature should not be substantially higher than the indicated ambient tem-
perature of +22 °C +/- 3 °C / 71.6 °F ± 5.4 °F to which the specified technical data relate. For
other ambient conditions, deviations from the indicated data are possible.
Avoid direct solar radiation on the unit.
•Permissible ambient humidity: 70% r.H. max., non-condensing.
•Installation height: max. 2000 m / 6561.7 ft above sea level. After the incubator has been turned on for
the first time, enter the altitude of the site above sea level into the RP1 controller (chap. 8.1).
•Wall distances: rear 100 mm / 3.94 in, sides 50 mm / 1.97 in.

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Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2warning system.
WARNING
High concentration of CO2(> 4 Vol.-%).
Danger of death by suffocation.
Danger of poisoning.
∅Do NOT set up units in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO2.
Observe the occupational exposure limit OEL for CO2set by the national authorities (formerly maxi-
mum permitted workplace concentration). Check compliance when operating all units located in the room.
•OEL for Germany: 5000 ml/m3 (ppm) = 0,5 Vol.-%
•CO2lost with each door opening: about 16.4 g, i.e. 0.0084 cubic meters / 0.296 cubic feet (under nor-
mal pressure)
•CO2lost during 12h at 5 vol.-% without door opening: approx. < 2 g, i.e. 0.001 cubic meters / 0.035
cubic feet (under normal pressure 1013 mbar / 14.7 psi)
An example of how to evaluate laboratory volume and air change rate:
Question: Is an air change rate of 1/h sufficient for a lab with a volume of 100 cubic meters / 3,531.5
cubic feet with 10 incubators C 170, opened 4 times per hour?
Calculation: CO2concentration = CO2lost by door opening, multiplied by 10 units, multiplied by 4 door
openings per hour, divided by lab volume
0.0084 cubic meters x 10 x 4 div. 100 cubic meters = 0.00336, i.e. 0.336% or 3360 ppm.
0.296 cubic feet x 10 x 4 div. 3,531.5 cubic feet = 0.00336, i.e. 0.336% or 3360 ppm.
Result: The maximum permissible value of 5000 ppm is not exceeded under these operation conditions.