BioLAB BMRW-104 User manual

Operation Manual
Microplate Washer
BMRW-104
Thank you for Choosing Biolab products. Please read the “Operating Instructions” and
“Warranty” before operating this unit to assure proper operation.

How to use this manual
It’s greatly appreciated you beco e the user of Microplate Washer.
Be sure to read this anual carefully before operating the instru ent.
In order to obtain the best result, it is i portant for you to get fa iliar with our instru ents
and their functions before perfor ing clinical diagnostic tests.
This User's Manual is the operation instructions for Microplate Washer, which including the
contents of instru ent installation, daily use, and aintenance etc.
Please reserve all the packing aterials for the use of deposit, transportation and
aintenance in the future.
You ay contact with your distributor if there is any proble .
As to the instru ents of different version or configuration, their functions are slightly
different.
Notes: Tips, advices and suggestions will be presented in italics for distinction.
Warning: The warning shall be strictly abided by in order to ensure that the instru ent ay
works nor ally and the test results ay be correct and authentic. Black letters are adopted for
distinction.
Symbols on the instrument
This sy bol eans that the labeled ite is hot while the instru ent is in use. Don’t
touch the labeled ite as you could be burnt.
The sy bol is labeled on the la p support of optic syste .
This eans that the labeled ite could lead to personal injury and/or da age to
the analyzer.
The sy bol is labeled beside the power outlet and so e external interface.
The sy bols for “SERIAL NUMBER”, The serial nu ber shall be after or below the
sy bol, adjacent to it.
The sy bol eans the product is in vitro diagnostic edical device.
The sy bol indicates the anufacturer and its address, after which are shown its
na e and address.
The sy bol indicates the date of anufacture, after which are shown its
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anufacture date.
The sy bol indicates EU representatives of the anufacturer and their addresses,
after which are shown their na es and addresses.
The sy bol indicates biological pollution, arked in the part where the instru ent
contacts the clinical reagent. The sy bol appears in black side and yellow
background.
The sy bol eans that the environ ent of instru ents ust be da pproof in the
course of transport, and instru ent ust be kept in a dry environ ent.
This eans that instru ent should handle with care in the course of transportation,
so as not to da age it.
The sy bol eans the instru ent packaged should not be upended at any ti e
The sy bol eans that the level piled up can't exceed 8 layers, as not to da age
instru ent.
The sy bol indicates te perature range of the analyzers during storage and
transportation.
Warning and Safety Indications
This instru ent is only used for in vitro diagnosis, please carefully read the following warnings
before the usage, and strictly obeys the .
• The articles and aterials used by so e test ite s have biological danger. Be sure to wear
the protective clothing and the glasses when operating the instru ent, and put down the
protection cover.
• So e steel pins of the wash head are sharp, so touching the ay lead to bodily injury.
Don’t put your hand or finger in the area of the worktable when the instru ent is in otion.
Be sure to turn off the power before replacing the wash head. It is forbidden to touch the
wash head when the instru ent is in otion.
• When the instru ent is in otion, be sure to turn off the power switch at once if the
waste bottle is turned over. At this o ent, the instru ent probably discharges a s all
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a ount of waste liquid through a device in the botto , and this waste liquid ust be dealt
with according to the biological dangerous cargo.
• When the incubator is being heated, don’t touch the incubation plate by hand in order to
avoid or prevent scald; further ore, the incubator adopts high-voltage power supply, so it is
forbidden to contact the incubation plate with hand or such conducting objects as ironware
etc. when the instru ent is in otion in order to avoid electric shock.
• Please wear the rubber gloves while carrying out aintenance; please wash your hands to
avoid virus infection after each operation finishes.
• Don't let hands or other positions of body touch the reagent or the patient's sa ple, if
the wounded part touches the patient’s sa ple carelessly, please wash it ti ely and see a
doctor for exa ination.
• Blocked wash head will cause bad aspiration and dispensing. In this case please rinse the
wash head with distilled water, and then dredge the blocked hole with a thin pin. If the
proble can not be solved this way, you need to replace the wash head.
• The product is a bench-type testing device, without issues on taboo.
• The ground wire of the power line of this instru ent has ground connection to avoid
electric shock. The ground wire has to be connected to ground.
• The AC electric supply ust be stable, and is forbidden to share the power with high-
power electric apparatus.
• While pulling out the power line, be sure to catch the plug, but not the power line.
• Turn off the power supply i ediately and contact the seller if the instru ent gives out
s og, peculiar s ell or strange sound.
• Be sure to cut off the power supply and switch off the ain processor when carrying out
aintenance or dis antling the outer cover due to other reasons.
• Phar aceutical affairs law and regulation: it is forbidden to refit the edical instru ent
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Index
How to use this anual............................................................................................................................2
Sy bols on the instru ent....................................................................................................................2
Warning and Safety Indications............................................................................................................3
1. Brief introduction of the instru ent.........................................................................................7
1.1 Brief introduction of product...............................................................................................7
1.1.1 Na e of the product: Microplate Washer.............................................................7
1.1.2 Features of the product.................................................................................................7
1.2 Specifications of the instru ent.........................................................................................7
1.3 External structure......................................................................................................................8
1.4 Keyboard....................................................................................................................................10
1.5 Principle of the instru ent.................................................................................................11
2. Installation of the instru ent...................................................................................................12
2.1 Unpack.......................................................................................................................................12
2.2 Install the bottles and tubing.........................................................................................................12
2.3 Install the wash head.........................................................................................................................13
2.4 Start the instru ent...........................................................................................................................13
3. Settings and adjust ent of the instru ent.....................................................................................14
3.1 Advanced settings..............................................................................................................................14
3.1.1 Adjust the LCD contrast ratio................................................................................................15
3.1.2 Hang-up settings.......................................................................................................................15
3.1.3 Plate shaking...............................................................................................................................16
3.1.4 Stroke settings............................................................................................................................17
3.1.5 Aspirate pu p speed settings..............................................................................................17
3.2 Plate settings........................................................................................................................................18
3.3 Switch bottle.........................................................................................................................................18
3.4 Turn off the syste ............................................................................................................................19
4. Operation of the instru ent..................................................................................................................20
4.1 Worktable and ELISA plates............................................................................................................20
4.2 Washing the plates.............................................................................................................................20
4.2.1 Para eter confir ation...........................................................................................................20
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4.2.2 Para eter settings.......................................................................................................................22
4.2.3 Plate washing.................................................................................................................................23
4.3 Single aspirate / single dispense.....................................................................................................24
4.3.1 Single aspirate................................................................................................................................25
4.3.2 Single dispense..............................................................................................................................25
4.4 Pri e / Rinse...........................................................................................................................................26
4.4.1 Pri e..................................................................................................................................................26
4.4.2 Rinse...................................................................................................................................................26
4.5 Incubator...................................................................................................................................................27
4.5.1 Para eter settings........................................................................................................................27
4.5.2 Incubation process........................................................................................................................27
5. Maintenance....................................................................................................................................................28
5.1 Regular aintenance of the worktable.........................................................................................28
5.2 Regular aintenance of the wash head........................................................................................28
5.3 Replace ent of the fuse......................................................................................................................28
5.4 Treat ent of the waste bottle...........................................................................................................29
6. Syste error and solutions........................................................................................................................29
Declaration.................................................................................................................................................................29
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Microplate Washer BMRW-104

01Brief introduction of the instru ent
1.1 Brief introduction of product
1.1.1 Name of the product: Microplate Washer
1.1. Features of the product
1) English operating syste , large screen liquid crystal display and graphic interface.
2) Support for single and three-channel wash syste apply to washes flat, U and V botto
ELISA plates and strips as well as standard 96-well or other types of plates.
3) Convenient progra ing, wash ti e, liquid volu e and soak ti e progra able. Plate
washing, row washing and row-skip washing available.
4) Auto atic positioning. Manual positioning to ensure the precision.
5) The design of double-pin wash head reduces cross conta ination.
6) Wash and waste bottles have auto alar function with liquid-surface detecting.
7) 8 and 12 way wash head available.
8) Incubation function (optional).
1. Specifications of the instrument
Wash head: 8-way or 12-way
No. of wash profiles 10
Capacity of the storage
bottle:
Wash bottle, distilled water bottle and waste
bottle, 2L each bottle respectively
Washing protocols 100
Washing cycles Max. 99
Soak / shaking ti e 0-24 h
Mode Plate & strip wash
Accuracy: +/- 5%
Residual volu e < 1 pl
Washing volu e 10-3000 pl/well
Resolution 1 pl
Incubator te perature 25° / 30° / 37.0°C fixed
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Te perature accuracy +/- 5 %
Te perature stability +/- 5 %
Te perature unifor ity +/-1 °C
War -up ti e 6 in. (a bient 25 °C to roo te perature)
Display Liquid crystal display
Interface RS-232 bi-directional co unication port
External di ensions 450 (L) X 390 (W) X190 (H)
Weight 13kg
Power supply a.c. 220V, 50Hz
Input power 80VA
Working environ ent 10°C~30°C, Relative hu idity <70%
Fuse T3.15AL 250V, 05X20
1.3 External structure
Fig. 1-1 Front view of instru ent
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1 ELISA plates 6 Wash bottle 3
2 Incubation cover 7 Distilled water bottle
3 Waste bottle 8 LCD screen
4 Wash bottle 1 9 Keyboard
5 Wash bottle 2 10 Transparent cover
Fig. 1-2 Back view of the instru ent
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1 Power switch 8 Interface 1 of waste bottle
2 Fuse 9 Interface of wash bottle 1
3 Power inlet 10 Interface of wash bottle 2
4 RS-232 interface 11 Interface of wash bottle 3
5 Sensor interface 12 Interface of distilled water bottle
6 Label 13 Air vent
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Wiring diagra of
tubing 14 Interface 2 of waste bottle
1.4 Keyboard
Fig. 1-3 Sche atic diagra of keyboard
START Start a process;
Save the para eter odifications.
CANCEL Cancel the current operation;
Discontinue the underway treat ent;
Cancel saving and quit the para eter odification.
AUpwards
V Downward
+ Change the para eters (increase, upwards, left)
— Change the para eters (reduce, downwards, right)
PRIME Start the pri ing procedure (shortcut key)
SELECT Switching between rough adjust ent / fine tuning
RINSE Start the rinse procedure (shortcut key)
ASPR Start the single aspirate / single dispense procedure (shortcut
key)
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Microplate Washer BMRW-104

1.5 Principle of the instrument
Microplate Washer is co posed of a wash bottle, a liquid aspirate pu p, a wash head, a
vacuu pu p and a waste bottle. Certain volu e of the liquid in the wash bottle is pu ped
by the liquid aspirate pu p, and then evenly dispensed into the icroplate by the wash head;
after special treat ent such as soak and shaking, etc. the waste liquid is pu ped to waste
bottle by vacuu pu p. Meanwhile, three wash channels of the instru ent are auto atically
switched over by a solenoid valve, and are controlled in real ti e by a relevant liquid surface
sensor which can feed back the liquid surface status of the current working bottle.
The wash bottle, liquid aspirate pu p and dispensing pin (thin etal pipe of the wash head)
ake up the dispensing routes of Microplate Washer; dispensing of the three kinds of the
wash liquid and the distilled water is controlled by four corresponding solenoid valves
respectively.
The liquid aspirate pin (relatively thick etal pipe of the wash head), the waste bottle and the
vacuu pu p ake up the draining routes of Microplate Washer. The negative pressure
generated by the vacuu pu p reaches the liquid aspirate pin via the waste bottle while the
liquid within the icrowells gets into the waste bottle under the at ospheric pressure.
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02Installation of the instru ent
First of all, for Microplate Washer, prepare a place which eets the following require ents:
• Environ ent te perature 10C-30C;
• Relative hu idity 70%;
• No direct sunlight or high dust concentration;
• No electro- agnetic radiation;
• With a sufficiently big, s ooth and fir tabletop.
.1 Unpack
Unpack the package and transportation aterials of the instru ent. Keep the packing box
and packing aterial for future storage and transportation over again.
• Take out the packing list, and check the accessories;
• Take out the instru ent.
Note: Keep the packing box and packing aterial for future storage and any later
transportation. Please contact the distributor if any accessory is issing.
. Install the bottles and tubing
Open the packing box of bottles, and take out the bottles, tubing, alar signal line, etc.
• Install the bottle: Towards the rear side of the instru ent, fro left to right sequentially
put the waste bottle, wash bottle 1, wash bottle 2, wash bottle 3 and distilled water bottle, as
shown in Fig. 1-1 and Fig. 2-1.
• Connect the tubing: As shown in Fig. 1-2 and 2-1, towards the rear side of the instru ent,
fro left to right, connect the tubing of the waste bottle, wash bottle 1, wash bottle 2, wash
bottle 3 and distilled water bottle according to the sequence of the bottles; A ong the , the
negative pressure port of the waste bottle is connected on the top of the tubing
(corresponding to the sa e interface color).
• Connect the signal line: As shown in Fig. 2-1, in the face of the rear side of the instru ent,
fro left to right, connect the signal lines of the waste bottle, wash bottle 1, wash bottle 2,
wash bottle 3 and distilled water bottle according to the sequence of the bottles.
Fig. 2-1 Wiring diagra of tubing and alar signals
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Microplate Washer BMRW-104

Note: The instru ent works with the principle of at ospheric pressure, so the bottleneck of
the waste bottle as well as all tubing interfaces ust be sealed up.
.3 Install the wash head
• Respectively rotate two ends of the dispensing tube and the draining tube to the
corresponding colored tube joints.
• Hang the wash head on the left side of the supporting bracket, and ake sure that the
two pins at side fall into the groove of the supporting bracket.
.4 Start the instrument
• Fill the wash bottles and the distilled water bottle with cleaning wash liquid and distilled
water respectively.
• Take out the power line in the accessories bag, and have one end connected to the power
inlet of the rear plate while the other end connected to the 220V power supply.
• Start the instru ent by pressing the power switch at the back of the instru ent.
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03Settings and adjust ent of the instru ent
In the software of Microplate Washer, a syste setting enu has been provided for users to
set up the syste . To ake sure that the instru ent works nor ally, you ust set up the
syste properly.
Note: If the syste settings are incorrect, it ay lead to the result that liquid spills over, plates
can not be washed effectively, or wash head be da aged, etc.
After starting the instru ent, at first it will initialize the syste atic para eters, and check if
the progra s need upgrading; Then the syste pri es the pipelines (for para eter setting,
please see Section 4.4) with preprogra ed wash liquid. At this o ent, press |CANCEL key
to cancel the pri ing process, or when the selected wash bottle has no liquid, the instru ent
will auto atically cancel the pri ing process and get into the ain interface; finally the
instru ent auto atically gets into the ain interface of the syste , as shown in Fig. 3-1. The
keys 0 s g, — can be pressed respectively to ake the enu selection of upwards,
downwards, right, and left, and the selected enu will be shown with highlight.
At the ain interface, pressing the |START| key will enter a corresponding enu procedure
while pressing the |CANCEL| key will show the syste LOGO. On the LOGO interface, pressing
any key will return to the ain interface of the syste .
The enu na es of ASPR/DISP, PRIME and RINSE indicates that the corresponding keys |
ASPR|, |PRIME|, |RINSE| on the keyboard can be used to enter the corresponding enu
directly.
Ver1.0e SN:XXXXXXXX
Plate washing Plate settings
ASPR/DISP Switch bottle
PRIME/RINSE Adv. settings
Incubator Switch off
Fig. 3-1 Main interface of the syste
3.1 Advanced settings
Set the syste atic para eters, such as LCD contrast ratio, work te perature of the incubator,
li it stroke of otor, and syste ’s hang-up ti e, etc. On the ain interface, the keys [a], [v], |
+| and — can be used to select "Adv. settings", and pressing key |START| will enter the
advanced settings progra , as shown in Fig. 3-2. The keys |a] and [v] are used to select the
corresponding progra s.
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Fig. 3-2 Advanced settings interface
3.1.1 Adjust the LCD contrast ratio
The keys [ ] and [v] can be used to select "Contrast ratio settings". And pressing key |START|
△
will enter the contrast ratio setting progra , as shown in Fig. 3-3.
Fig. 3-3 Interface of contrast ratio settings
If the key |+| is pressed the contrast ratio increases and the color beco es darker; if the key —
is pressed the contrast ratio decreases and the color beco es lighter. After adjusting to
opti u value, press |START| to finish the adjust ent and get back to previous enu. The
new value of contrast ratio is saved in the FlashROM of Microplate Washer. When the
instru ent started next ti e, the LCD contrast ratio is set up according to the new value.
During adjust ent, press [CANCEL to quit without saving.
Microplate Washer has 64 contrast ratio grades.
3.1. Hang-up settings
User can set up the hang-up ti e. Keys Q and - are used to increase or decrease ti e;
Pressing (SELECT can switch between the operation of changing the ti e by 1 or 10 inutes.
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Contrast ratio settings
Hang-up settings
Plate shaking
Stroke settings
Asp. pump speed settings

Press |START| to save and quit, and press the key |CANCLE| to quit without saving, as shown in
Fig. 3-4.
If there is no operation within the preprogra ed hang-up ti e, instru ent will rinse the
pipeline auto atically and get into a hang-up status. In the hang-up status, press any key to
quit and get back to operation; when returned fro hang-up status, the instru ent will
auto atically pri e pipelines with preprogra ed wash liquid.
3.1.3 Plate shaking
The user can shake plate during washing procedure or independently. The and keys are
 
used to select "Plate shaking". Press START to enter the Plate shaking interface as shown in
Fig. 3-5.
Fig. 3-5 Plate shaking
Use the and keys to select Ti e, Speed, and Extent of plate shaking. And Press + and – to
 
adjust values. Shaking intensity and ti e are adjustable. There are 10 levels of Speed, the
bigger value, the faster. Extent is the distance of oving back and forth, can be set 0.1-2.0 .
Maxi u shaking ti e is 23H 59M 59S. Intensity of plate shaking in Plate washing enu
depends on intensity set on Plate shaking enu. In plate shaking enu, incubation settings is
also available. For ore details about incubation, you can reference to chapter 4-5. Maxi u
incubation ti e is 18h. Plate shaking and incubation can be carried out independently.
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Press START to save changes and shake plate. Shaking ti e is displayed. Double press SELECT
key to start counting of incubation ti e. Press CANCEL to quit.
3.1.4 Stroke settings
User can adjust the stroke scope of otor on horizontal and vertical directions. Keys and
△ ▽
are used to select "Stroke Settings", and press START to enter the stroke settings progra , as
shown in Fig. 3-6.
Keys and are used to select "Horizontal direction" or "Vertical direction". Keys + and –
△ ▽
are used to adjust the axi u stroke scope.
Press START to save the changes, at the sa e ti e, the corresponding otor oves to the
preprogra ed position auto atically.
Press CANCEL to quit without saving
3.1.5 Aspirate pump speed settings
Keys and are used to select “Asp. pu p speed settings", and press START to enter the
△ ▽
pu p settings progra , as shown in Fig. 3-7. Through keys ＋ and －to set suitable pu p
speed value. Key ＋ is used to open the pu p; Key － is used to close the pu p, and the
status bar will show “on” or “off” respectively.
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3. Plate settings
User can preprogra 10 kinds of plate para eters. Keys , , + and – are used to select
△ ▽
"Plate Settings", and pressing START will get into the plate settings progra , as shown in Fig.
3-8.
and are used to select the para eters which need odification, and keys + and – are
△ ▽
used to odify relevant para eter values; Press START to save and return, and press key
CANCLE to quit without saving.
The para eter description and the specific odification operation are stated as follows:
1. Plate No.: Code nu ber of plate type, selected between 1~10;
2. Wash head: 8/12 way wash head can be selected, and the default value is 12;
3. Plate settings: Flat-type (F), U-type (U) and V-type (V) can be selected, and the letters in the
brackets are their code nu bers. The default setting is Flat-type (F);
4. Center: The centre position. Corresponds to the centre position of the first strip of
icrowells. Keys + and – are used for adjust ent by 0.1 ;
5. Top: Position on the top. Corresponds to the top plane (top aspirate position) of the first
strip of icrowells. Keys + and – are used for adjust ent by 0.1 ;
6. Botto : Position at the botto . Corresponds to the botto position (liquid dispensing
position) of the first strip of icrowells. Keys + and – are used for adjust ent by 0.1 .
7. Bot. wash: Botto washing position. Corresponds to the iddle position (botto wash
position) in the vertical direction of the first strip of icrowells. Keys + and – are used for
adjust ent by 0.1 ;
8. Left: Left position. Corresponds to the left position of the first strip of icrowells. Keys +
and – are used for adjust ent by 0.1 ;
9. Right: Position on the right side. Corresponds to the right side position of the first strip of
icrowells. Keys + and – are used for adjust ent by 0.1 ;
3.3 Switch bottle
Microplate Washer can switch between three wash bottles conveniently, keys , , + and –
△ ▽
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Microplate Washer BMRW-104

are used to select "Switch bottle", and pressing START will enter the progra of switching
wash bottles, as shown in Fig. 3-9.
Keys and are used to select bottle nu ber or switching ti e; The keys + and – are used
△ ▽
to odification the switching ti e. When the bottle nu ber change, in the botto of the
interface shows "switched to bottle X#", as shown in Fig. 3-9 (b). Press START to start
switching the wash bottle; otherwise current bottle nu ber re ains, as shown in Fig. 3-9 (a),
wash bottle will not be switched. During switching procedure, first of all, rinsed the pipelines
with distilled water. About the rinse ti e settings, please refer to section “4.4.2 Rinse”. The
status shows "Rinse X"; and then pri e the pipelines with the wash liquid fro the target
wash bottle. The status shows "Pri e X". During operation, pressing key CANCEL will cancel
the switching. It will keep using the wash liquid fro current wash bottle.
3.4 Turn off the system
If it is confir ed that the plate washing operation has been co pleted, user can directly
switch off the instru ent; or the user can select "Switch off " progra on the ain enu, then
the syste auto atically rinses the pipelines with distilled water, saves the current progra
code nu ber of the plate washing progra , and ask user to switch off the syste , as shown
in Fig. 3-10.
Press START to switch off; press key CANCLE to return to the ain enu of syste and
continue with operation.
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04Operation of the instru ent
4.1 Worktable and ELISA plates
The waiting groove is in the iddle of the worktable. When the instru ent at stand-by status,
the wash head stops in the waiting groove; in front of the groove is a carrying table used to
place the ELISA plates.
Microplate Washer supports ELISA plates of specifications such as 12x8, 12x4, and 8x6, etc.,
but different wash heads ay have li itations to different ELISA plates. The detailed relation
is as follows:
When installing the ELISA plates, pay attention to the direction of the ELISA plates. If 8-way
wash head is used, the edges with 8 icrowells of the ELISA plate shall be put parallel to the
wash head. Si ilarly, when 12-way wash head is used, the edges with 12 icrowells of the
ELISA plate shall be put parallel to the wash head.
When installing the ELISA plates, with your right hand catch the two sides of the long edge of
the ELISA plate, and fro top to botto , put it into the cross groove, and ake the rear edge
of the ELISA plate closely contact the rear edge of the nearest groove.
4. Washing the plates
4. .1 Parameter confirmation
Use , , + and – to select "Plate washing", press START to enter the para eter confir ation
△ ▽
progra , as shown in Fig. 4-1. Fro top to botto and left to right, the para eters are as
follows:
1. Progra No.: Code nu ber of the current progra , use + and – to select progra . Press
SELECT to switch between changing progra nu ber by 1 or 10.
2. Settings: If the para eters of the current progra need odification, the para eters can
be
edited in the "Settings" enu;
3. 1(12-F): Means that the plate type setting with code nu ber of "1" is selected; "12" eans a
12-way wash head while "F" eans that the position of this progra is set up according to F
type;
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