Boekel Bambino 230301 User manual

Boekel Bambino™
Hybridization Oven
Models
230301 and 230301-2
Operating Instructions
N2400204 Rev 0 11/03

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CONTENTS Page
1. Safety................................................................................................................2
2. Product Information...........................................................................................3
3. Assembly..........................................................................................................3
3.1 Unpacking .....................................................................................................3
3.2 Installation.....................................................................................................3
4. Operation.........................................................................................................3
4.1 Controls and Indicator lamps (see figures 1 & 2)...........................................3
4.2 Setting the temperature.................................................................................3
4.3 Loading Carousel..........................................................................................4
4.4 Spindle Adjustment .......................................................................................4
4.5 Removing/Installing Carousel........................................................................4
4.6 50ml Conical Centrifuge Tubes.....................................................................4
5. Accessories......................................................................................................4
5.1 Multi-Tube Carousel......................................................................................4
5.2 Clips for Multi-Tube Carousel (22 clips per bag) ...........................................5
6. Fault Diagnosis ................................................................................................5
7. Technical Specifications...................................................................................5
8. Maintenance and Service.................................................................................6
8.1 Cleaning........................................................................................................6
8.2 Replacement of Fuses ..................................................................................6
9. Warranty...........................................................................................................6
10. Service.............................................................................................................6

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1. Safety
The following symbols marked on the equipment mean:
Caution: Read these operating instructions fully before use and pay particular
attention to sections containing this symbol.
Caution: Surfaces can become hot during use.
Always observe the following safety precautions:
Use only as specified by the operating instructions or the intrinsic protection may
be impaired. After transport or storage in humid conditions, dry out the
unit before connecting it to the supply voltage. During drying out the
intrinsic protection may be impaired.
RISK OF TRAPPING: Do not attempt to add or remove tubes from the
Carousel or adjust the clips until the motor has stopped.
Connect only to a power supply with a voltage corresponding to that on the
serial number label.
Connect only to a power supply that provides a safety ground terminal.
Before moving, disconnect at the power supply socket. Do not remove the plug.
Do not check the temperature by touch, but instead use the temperature display
or a thermometer.
To reduce the risk of eye injury during high temperature operation, use safety
goggles or spectacles.
Do not touch surfaces that become hot during high temperature operation.
Ensure that the operating temperature is less than the maximum operating
temperature of your sample material.
Ensure that the power switch is easily accessible during use.
Do not block or restrict ventilation slots.
If liquid is spilled inside the unit, disconnect it from the power supply and have it
checked by a competent person.
It is the user’s responsibility to carry out appropriate decontamination if
hazardous material is spilled on or inside the equipment.

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2. Product Information
The Boekel Hybridization Oven Model 230301 & 230301-2 hybridization oven will
hold up to four 50ml plastic centrifuge tubes. The Oven can also be used with other
size tubes using the optional carousel.
The Boekel Hybridization Oven utilizes an adjustable speed Carousel and forced
air circulation coupled with proportional (PID) temperature control that combine to
provide unsurpassed temperature performance and the most consistent conditions
from procedure to procedure. Please note that the convection fan operates
continuously whenever the unit is turned on.
3. Assembly
3.1 Unpacking
Remove packing materials carefully, and retain for future shipment or storage of
the unit. Inspect for damage. Report all shipping damage to the carrier
immediately. Shipping damage is covered by the carrier and repair/replacement
for shipping damages must be coordinated through the carrier. Complete and
return the Warranty Registration Card. Packs should contain:
Hybridization Oven
Power line cord
Operating instructions
3.2 Installation
Place the Oven on a flat and stable surface, preferably away from drafts. Fit the
power line cord into the IEC power socket on the rear of the unit.
4. Operation
4.1 Controls and Indicator lamps (see figures 1 & 2).
The Power Switch controls power to the unit.
The temperature display shows the chamber temperature in degrees Celsius.
The Speed Control knob regulates the speed of the Carousel.
The heater lamp is on continuously while the Oven is heating up. As the required
temperature is approached, it starts to flash. When the unit is controlling at the
set temperature, the heater lamp flashes intermittently.
4.2 Setting the temperature
The temperature controller has three buttons. When the button on the left ‘*’ is
depressed, it will display the set temperature. When the left button ‘*’ is
depressed simultaneously with the middle button ‘▼’, the set temperature value
is lowered. When the left button ‘*’ is depressed simultaneously with the right

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button ‘▲’, the set temperature value is raised. When all buttons are released,
the actual chamber temperature is displayed.
In the event of power loss, the Temperature Controller retains the last set
temperature value.
4.3 Loading Carousel
Warning! Sharp rotating parts. Do not attempt to add or remove tubes until the
motor has stopped. The motor can be stopped by turning the power switch to the
“Off” position or by depressing the “Pause” button located on the back of the unit,
(see figure 2). When loading the Carousel, it is recommended that the load be
evenly distributed. Tubes should be positioned with sufficient clearance from the
chamber wall to avoid interference from the screw heads during tube rotation.
4.4 Spindle Adjustment
If desired, it is possible to adjust the Spindle, which imparts a “rocking” or 3-D
motion to get an additional washing action to the tube. The Clips have been
factory set to position the tubes horizontally, but this can be adjusted by
loosening the screws securing the clips, rotating them slightly, then re-tightening
the screws to get the desired effect. Warning! Remove power from the unit
before adjusting.
4.5 Removing/Installing Carousel
Warning! Remove power from the equipment before removing or installing the
Carousel. To remove Carousel, first remove all tubes. Position Carousel so the
socket head screw on the shaft (located on the left side of the drive shaft) is
accessible, and turn power off. Loosen socket head screw with a 1/16 inch hex
key and slide the carousel to the right. To install Carousel, reverse this
procedure.
4.6 50ml Conical Centrifuge Tubes
Be sure to use tubes that can handle the elevated temperatures used in
hybridization. We recommend that you contact your supplier or tube
manufacturer. In general, scientists have found that tubes made from
polypropylene with polyethylene caps provide reliable performance. Tubes made
from polystyrene should be avoided.
When inserting the centrifuge tube into the clamping mechanism, place the
clamp toward the middle of the tube. Pinching the tube near the cap may cause
a leak.
5. Accessories
5.1 Multi-Tube Carousel
Catalog Number 230332

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5.2 Clips for Multi-Tube Carousel (22 clips per bag)
four clips for 50ml tubes
six clips for 15ml tubes
twelve clips for 1.5/2.0ml tubes
Catalog Number 230350
6. Fault Diagnosis
Symptom
Possible Cause
Action Required
1. Unit does not operate
a. Unit not switched on
a. Switch on
b. Unit not plugged into
power supply
b. Plug in, switch on
c. Fuses blown
c. Replace fuses per 8.2
d. Power supply failure
d. Check that other
electrical appliances on
the same circuit are
working
2. Chamber temperature
does not rise when
expected
a. Actual temperature is
higher than set
temperature
a. Check set temperature
b. Temperature control
circuit fault
b. Have unit checked by
competent person
3. Temperature continues
to rise when not
expected
a. Actual temperature is
lower than set
temperature
a. Check set temperature
b. Temperature control
circuit fault
b. Have unit checked by
competent person
4. No rotation, LEDs
illuminated
a. An obstruction prevents
Carousel rotation
a. Remove obstruction
b. Carousel is loose
b. Tighten set screw with
hex key
7. Technical Specifications
This equipment is for indoor use and will meet its performance figures within the
ambient temperature range of 10°C to 35°C, with maximum relative humidity of 80%
non-condensing. Installation category II (transient voltages). Pollution degree 2 in
accordance with IEC 664. For operation at altitudes of up to 6500 feet.
Temperature range (Ambient +10°C) to 80°C.
Setting range 10°C to 80°C
Stability +/- 0.1°C
Overall Accuracy +/- 0.5°C at 37°C
Temperature display resolution 0.1°C
Supply voltage range Catalog No. 230301 – 115V +/- 10%, 60 Hz.
Catalog No. 230301-2 – 230V +/- 10%, 50/60 Hz.
Power Rating Model 230301 & 230301-290W

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Heating Rate Ambient to 70°C within 25 minutes
Speed of Rotation 6 to 20 RPM
Note: A minimum of at least two tubes are needed for optimum temperature
stability.
8. Maintenance and Service
The Model 230301 & 230301-2 Oven is designed to comply with IEC1010-1.
No routine maintenance is required.
8.1 Cleaning
Disengage power cord prior to cleaning. If a spill occurs, use appropriate clean
up procedures as required for radiation or biohazard control. The outer casing
can be cleaned with a damp cloth. Do not submerge or immerse the Oven in
water.
8.2 Replacement of Fuses
Disconnect the unit from the power supply.
Remove the plug from the socket in the back of the unit.
Pull back on the fuse drawer catch.
Pull out the fuse drawer. (See Figure 2)
Check and replace with the correct fuses if necessary. The fuses should be
5mm x 20mm quick acting, rated 250V.
230301 (115V) 1.0AF
230301-2 (230V) 0.5AF
Push the fuse drawer back in. Reconnect unit to the power supply.
9. Warranty
When used in laboratory conditions and according to these operating instructions
Boekel warrants this product to be free of defective material and workmanship for a
period of two years from the date of manufacture. The liability of Boekel for any
defective equipment during the warranty period shall be limited to the repair of
such equipment or replacement thereof without charge for parts or labor.
10. Service
Should service be required, call Boekel Scientific Customer Service. It is required
to obtain a Returned Material Authorization (RMA) number before any product is
returned for any reason. A Decontamination Certificate must be completed, signed
by the user, and returned to Boekel Scientific prior to receiving the RMA number.
Please be sure to mark the outside of the returned goods package with this RMA
number to ensure prompt handling.
Boekel Scientific PHONE: [phone] or [phone]
855 Pennsylvania Blvd. FAX: [phone]
Feasterville, PA 19053 e-mail: [e-mail]

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Drawer
Fuse
I
Switch
Power
Knob
Control
Speed
Button
Pause
Figure 2
Figure 1
Lamp
Heater
Display
Temperature