Bruker EPSON Perfection V850 Pro User manual

EPSON Perfection V850 Pro
Quick Guide
Copyright © 2021
Doc. No. 5046693 Revision A (July 2021)
Language: en
1888343

Legal and Regulatory Notices Bruker
Legal and Regulatory Notices
Read this section before proceeding to the rest of the sections.
Copyright © 2021
Bruker Daltonics GmbH & Co. KG
All rights reserved
Reproduction, adaptation, or translation without prior written permission is prohibited,
except as allowed under the copyright laws.
Warranty
The information contained in this document is subject to change without notice.
Bruker Daltonics GmbH & Co. KG makes no warranty of any kind with regard to this
material, including, but not limited to, the implied warranties of merchantability and
fitness for a particular purpose.
Bruker Daltonics GmbH & Co. KG is not liable for errors contained herein or for
incidental or consequential damages in connection with the furnishing, performance or
use of this material.
Bruker Daltonics GmbH & Co. KG assumes no responsibility for the use or reliability of
its software on equipment that is not furnished by Bruker.
Use of trademarks
The names of actual companies and products mentioned herein may be the trademarks
of their respective owners.
Limitations on use
For Research Use Only (RUO)
This product has no declared clinical intended purpose and is not for clinical diagnostic
use. Any clinical diagnostic use is at the user’s own risk and responsibility.
Descriptions and specifications supersede all previous information and are subject to
change without notice.
Hyperlink disclaimer
Bruker Daltonics GmbH & Co. KG makes no express warranty, neither written nor oral,
and is neither responsible nor liable for data or content from the linked Internet
resources presented in this document.
Page 2 of 23 Quick Guide EPSON Perfection V850 Revision A

Bruker Document History
Document History
Title: Quick Guide EPSON Perfection V850
Revision: Revision A (July 2021)
Document number: 5046693
First revision: July 2021
The following table describes important changes from the previous revision of this
document.
List of changes
No changes: First revision
Quick Guide EPSON Perfection V850 Revision A Page 3 of 23

Manufacturer Bruker
Manufacturer
Bruker Daltonics GmbH & Co. KG
Fahrenheitstr. 4
28359 Bremen
Germany
Phone: [phone]
Contact your local Bruker representative for service and further information.
Germany USA
Bruker Daltonics GmbH & Co. KG
Fahrenheitstrasse 4
28359 Bremen
Germany
Phone: : [phone]
Internet: www.bruker.com
Bruker Scientific LLC
40 Manning Road
Billerica, MA 01821
USA
Phone: [phone]
Internet: www.bruker.com
Application Support
Phone: [phone]
Email: [email protected]
Service and Support
Phone: [phone]
Technical Support
Phone: [phone]
Email: [email protected]
Service Support
Phone: [phone]
Email: [email protected]
Page 4 of 23 Quick Guide EPSON Perfection V850 Revision A

Bruker Table of Contents
Table of Contents
Legal and Regulatory Notices 2
Document History 3
Manufacturer 4
Table of Contents 5
1 Overview 6
2 Site Specifications 7
3 System Requirements 8
4 Installation 9
5 Create a Scan 10
6 Troubleshooting 19
7 Service Information 22
Index 23
Quick Guide EPSON Perfection V850 Revision A Page 5 of 23

1 Overview Bruker
1 Overview
This document provides information on how to use the EPSON Perfection V850 Pro
scanner and EPSON Scan software for creating pre-measurement scans that can be
used for the automatic workflow and SCiLS™ autopilot in Bruker’s flexImaging software.
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Bruker 2 Site Specifications
2 Site Specifications
lEPSON Perfection V850 Pro (Bruker cat #1888343)
oEPSON Scanner Driver and EPSON Scan Utility v3.9.3.4 (download from
EPSON).
lIntelliSlide (Bruker cat #1868957) with sample.
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3 System Requirements Bruker
3 System Requirements
lCPU: Pentium Core processor equivalent or better, clock frequency 1.6 GHz or
more for satisfying data handling (Recommended: dual core workstation or better
for acquisition).
lHard disk: at least 12 GB of free disk space
lMain Memory: minimum 8 GB RAM. For handling large data sets, it is
recommended to have 32 GB RAM or more.
lOperating System: Windows 10 64bit, English versions
lGraphic resolution: 1280x1024 pixels with true colors
lInterface: USB 2.0 (Type-B standard connector)
Note The setup program could change the settings of the Windows firewall to allow
outgoing passive FTP connections to ftp.bdal.de for the automatic update
notification feature. This change only affects systems where the default
firewall settings had been modified.
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Bruker 4 Installation
4 Installation
You need administrator privileges for all installation/uninstallation tasks.
Please make sure that the scanner device is connected to the computer where this
software will be installed.
1. Download the Scanner Driver and EPSON Scan Utility v3.9.3.4 from EPSON
2. Click V800_V850_3934_AM.exe and allow the program to make changes to your
computer.
3. Follow the installation wizard instructions to set up EPSON Scan on your computer.
4. Click Finish when you get to the end of the wizard prompts.
Note Should you have difficulties installing the software due to anti-virus settings,
please contact your server administrator for help.
Quick Guide EPSON Perfection V850 Revision A Page 9 of 23

5 Create a Scan Bruker
5 Create a Scan
When using the EPSON Perfection V850 Pro to create high resolution scans of your
slides, it is important to take note of the following:
oCreate your optical pre-measurement scan before matrix coating.
While the IntelliSlide sprayer mask will keep the barcode, Bruker logo, and teach
marks at the far edges free of matrix, visualization of tissue regions is hindered
by matrix coating.
oThe scan should be created with the slide facing upwards.
This will prevent your sample from touching the surface of the scanner. The slide
is facing upwards if the inscribed numbers and Bruker logo are legible.
1. Switch on the scanner and open the EPSON Scan software.
2. Make sure that the sample (medium format) adapter has been placed on the
scanning surface of the EPSON Perfection V850 Pro, with the white triangles on the
holder and the scanner are aligned (see Figure 1-1).
3. Adjust the height of the sample adapter to the lowest setting with the small slide
controls, as indicated by the red arrows and rectanglesFigure 1-1. All slide controls
of the holder must be adjusted to the same setting.
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Bruker 5 Create a Scan
Figure 1-1 EPSON Perfection V850 with sample film adapter on scanning surface
Note Please note the alignment of the white triangles with each other at the right
edge of the adapter and the height adjustment setting of the adapter (red
boxes and arrows).
4. Place your slide onto scanner surface into one of the corners of the adapter with the
slide facing upwards.
5. Close the scanner lid.
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5 Create a Scan Bruker
6. In the EPSON Scan software, make sure you have applied the following settings:
lMode: Professional Mode
lDocument type: Film (with Film Holder)
lImage Type: 24-bit Color
lResolution: 3200 dpi
lScale: 100%
Figure 1-2 EPSON Scan software showing the recommended scan settings
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Bruker 5 Create a Scan
7. Click the “Preview” button on the bottom left
lCreation of a low-resolution preview scan of your sample starts.
lTwo new windows open:
lProgress window showing a green progress bar.
lPreview window showing the preview image.
Note If an EPSON Scan window with the statement “Cannot find a document.
Preview is displayed in Normal mode” appears after completion of scan, click
‘Close’.
lA low-resolution scan of the sample appears automatically in the Preview window
8. Hold down the left mouse button and drag over the IntelliSlide in the Preview
window.
lA dotted line box will form around the low-resolution scan of your slide.
lThis dotted box defines the region of interest (ROI) for the high-resolution scan.
9. To scan the entire IntelliSlide, set the Document Size and Target Size parameters in
the EPSON Scan software to W: 24.4 mm, H: 74.2 mm.
Note The precise selection of these dimensions covering the entire IntelliSlide are
important as barcode reading will only work if the inscribed features are not
cropped.
The barcode reading may also fail if the area for the slide is too large. Ideally
the scan border is just inside the edge of the slide.
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5 Create a Scan Bruker
Figure 1-3 EPSON Scan software showing low resolution preview in Prescan Image
tab, and dotted line box indicating scan area
10. Click “Zoom” in the Prescan window to check that the ROI defined for the high-
resolution scan encompasses the entire IntelliSlide without cropping any of the
inscribed features.
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Bruker 5 Create a Scan
Figure 1-4 Zoomed view of the defined ROI for the high-resolution scan
Note If the dotted box crops some of the inscribed features, hold down the left
mouse button to move the dotted box so that the features are included in the
area.
Note The image in the preview window and the scan output will be a mirror-image
of the slide.
11. Click “Histogram Adjustment”
12. Change the input values in the newly opend dialog to 154 for the black, 1.34 for the
gray and 232 for the white channel
lThe output should range from 18-255.
13. Close the dialog.
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5 Create a Scan Bruker
Figure 1-5 Histogram adjustment settings
Note The adjusted histogram settings are important for the automatic tissue
recognition during the SCiLS™ autopilot.
14. Click the “Scan” button to create a high-resolution scan of the area defined in the
Prescan tab.
lA File Save Settings dialog box opens.
15. Use the settings shown in Figure 1-6.
16. Click "OK" to commence scanning.
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Bruker 5 Create a Scan
Figure 1-6 High resolution scan in Scan Image Tab
Note The folder in which your image is saved should open immediately after the
scan is complete.
lThe scan output from the EPSON Perfection V850 Pro will be a mirror-image of
the slide
17. Rotate the image (horizontal flip) before use via Windows applications such as
Photos, Paint, or Paint 3D for the correct orientation
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5 Create a Scan Bruker
Figure 1-7 Location of ‘flip’ function in Windows Photos
Figure 1-8 Correct orientation of a scanned IntelliSlide (without sample)
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Bruker 6 Troubleshooting
6 Troubleshooting
Incorrect definition of the high- resolution scan ROI can produce scans that are
incompatible with the automated workflows in Bruker’s flexImaging software.
The following are examples of scans resulting from incorrect ROI definition.
Figure 1-9 Scan created using ROI larger than IntelliSlide
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6 Troubleshooting Bruker
Figure 1-10 Scan created using ROI smaller than IntelliSlide, resulting in cropping of
barcode, serial number and Bruker logo
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