Bruker BioSpec 70/30 USR V2 User manual

BioSpec 70/30 USR
Site Planning Information
with Magnet B-C 70/30 USR V2
Version V020
Innovation with Integrity
●
Preclinical Imaging

Copyright © by Bruker Corporation
All rights reserved. No part of this publication may be reproduced, stored in a retrieval
system, or transmitted, in any form, or by any means without the prior consent of the
publisher. Product names used are trademarks or registered trademarks of their re-
spective holders.
This manual was written by
Bruker
© February 21, 2018
Document Number: 9007201111482251
P/N: Site Planning Information BioSpec 70/30 USR
For further technical assistance for this product, please do not hesitate to contact your
nearest BRUKER dealer or contact us directly at:
D-76275 Ettlingen
Germany
Phone:
E-mail:
Internet: www.bruker

Contents
Site Planning Information BioSpec 70/30 USR_2_V020 iii
Contents
1 Introduction.........................................................................................................................................7
1.1 About this Manual ...............................................................................................................7
1.2 Overview .............................................................................................................................7
1.3 Additional Documents .........................................................................................................7
1.4 Responsibilities ...................................................................................................................8
1.5 Validity ................................................................................................................................8
2 Safety...................................................................................................................................................9
2.1 Safety during the Installation Phase ...................................................................................9
2.2 Instructions to plan a safe and compliant MR Site............................................................12
2.2.1 Magnetic Field...................................................................................................................12
2.2.1.1 Controlled Access Area ....................................................................................................13
2.2.1.2 Exposed Area ...................................................................................................................14
2.2.1.3 Exhaust system.................................................................................................................14
2.2.2 Cryogenic Fluids and Gas.................................................................................................14
2.2.3 Oxygen Supervision..........................................................................................................15
2.2.4 Emergency Plan................................................................................................................15
2.2.5 Fire Prevention..................................................................................................................16
2.2.6 Biological Safety ...............................................................................................................16
2.2.7 Seismic Safety ..................................................................................................................16
2.2.8 Safety at Work ..................................................................................................................16
2.2.8.1 USER WORK SAFETY DS - Isoflurane - Rev0 ................................................................18
2.2.9 Infrastructure Supervision and Messaging........................................................................19
3 Standards ..........................................................................................................................................21
3.1 Standards..........................................................................................................................21
3.2 Environmental Conditions .................................................................................................21
3.3 National Standards Operating MR Instruments ................................................................22
4 Installation Schedule........................................................................................................................23
4.1 Installation planning overview ...........................................................................................23
4.2 Planning aids ....................................................................................................................25
4.2.1 Installation Schedule.........................................................................................................25
4.3 Prerequisites for delivery ..................................................................................................25
4.3.1 Giving notice of readiness for shipment............................................................................25
4.3.2 Prerequisites for Delivery..................................................................................................25
4.3.3 Prerequisites for Operation ...............................................................................................26
5 Laboratory Infrastructure and Interactions....................................................................................27
5.1 Operational Workflow........................................................................................................27
5.2 IT Structures .....................................................................................................................27
5.3 Interactions .......................................................................................................................28
5.3.1 Magnetic interactions ........................................................................................................28
5.3.1.1 Ferromagnetic mass .........................................................................................................28
5.3.1.2 Mobile sources of magnetic disturbance...........................................................................29

Contents
iv Site Planning Information BioSpec 70/30 USR_2_V020
5.3.1.3 Effect of MR system to other equipment...........................................................................30
5.3.1.4 Remote magnetic effects ..................................................................................................30
5.3.2 Electromagnetic interactions.............................................................................................30
5.3.2.1 DC and LF interference.....................................................................................................31
5.3.2.2 RF interference .................................................................................................................32
5.3.3 Mechanical interactions ....................................................................................................34
5.3.3.1 Ground and building vibrations .........................................................................................34
5.3.3.2 Impact noise......................................................................................................................36
5.3.3.3 Acoustic ............................................................................................................................36
6 Planning Details................................................................................................................................37
6.1 Measurements and room dimensions...............................................................................37
6.1.1 Overview ...........................................................................................................................37
6.1.2 Operating area ..................................................................................................................38
6.1.3 Magnet Area .....................................................................................................................38
6.1.4 Technical area ..................................................................................................................41
6.2 RF Shielded Room............................................................................................................42
6.2.1 Filter plates .......................................................................................................................43
6.2.1.1 Electronic filter plate..........................................................................................................47
6.2.1.2 Magnet filter plate .............................................................................................................47
6.2.1.3 Filter plate for anesthetic gas exhaust ..............................................................................47
6.2.1.4 Filter plate for magnet exhaust system .............................................................................48
6.2.1.5 MRI CryoProbe™ filter plate .............................................................................................48
6.2.1.6 In-vivo filter plate...............................................................................................................48
6.2.1.7 Filter plate for Faraday cage ventilation............................................................................48
6.3 Exhaust system.................................................................................................................49
6.3.1 Design criteria of the exhaust system ...............................................................................50
6.3.2 Calculating the exhaust system ........................................................................................52
6.4 Floor construction .............................................................................................................54
6.4.1 Footprint and weights........................................................................................................54
6.4.2 Magnet foundation ............................................................................................................55
6.4.3 Electrostatic discharge......................................................................................................57
6.4.4 Seismic safety standards ..................................................................................................57
6.5 Electrical installations........................................................................................................59
6.5.1 Overview ...........................................................................................................................59
6.5.2 Mains Supply ....................................................................................................................59
6.5.3 Equipotential Bonding .......................................................................................................62
6.5.4 Electrical connections in the magnet room .......................................................................62
6.5.5 Electrical connections in the operating room ....................................................................62
6.5.6 Line Power Distributor.......................................................................................................63
6.6 Supervision signals ...........................................................................................................64
6.7 Cable lengths and routing .................................................................................................64
6.7.1 Line lengths for the MRI CryoProbe..................................................................................68
6.8 Lighting system .................................................................................................................69
6.9 Ventilation and air conditioning .........................................................................................69
6.9.1 Overview ...........................................................................................................................69
6.9.2 Air conditioning systems ...................................................................................................70
6.9.3 Anesthetic gas extraction..................................................................................................71

Contents
Site Planning Information BioSpec 70/30 USR_2_V020 v
6.10 Cold Water Supply ............................................................................................................72
6.10.1 Connections and installation .............................................................................................74
6.11 Cryogenic Fluids ...............................................................................................................76
6.12 Helium Gas and Compressed Air Supply .........................................................................76
6.12.1 MRI CryoProbe™..............................................................................................................76
6.13 Laboratory Furnishings .....................................................................................................78
6.13.1 Laboratory Furniture .........................................................................................................78
7 Delivery and Transport ....................................................................................................................79
7.1 Maximum transport time ...................................................................................................80
7.2 Packaging .........................................................................................................................80
7.3 Magnet Transport..............................................................................................................81
7.3.1 Transport method and limitations......................................................................................81
7.4 Transporting Electronic Cabinets......................................................................................83
7.5 Moving into the building ....................................................................................................83
7.6 Moving under special circumstances ................................................................................86
8 Moving or Dismantling the MR Instrument ....................................................................................87
9 Checklist in Preparation of the Installation....................................................................................89
9.1 Customer information........................................................................................................89
9.2 Magnet installation ............................................................................................................90
9.3 Laboratory rooms features................................................................................................91
9.4 Declaration........................................................................................................................93
Index ..................................................................................................................................................99

Contents
vi Site Planning Information BioSpec 70/30 USR_2_V020

Introduction
Site Planning Information BioSpec 70/30 USR_2_V020 7
1 Introduction
This manual provides support for the system owner when planning installation and meeting
the installation requirements of an MR system by specifying planning data.
1.1 About this Manual
This manual is to be used by the System Owner (responsible body) and planning offices that
have been entrusted with the site planning of the installation of the MR instrument. It provides
the installation requirements of the MR instrument.
This manual does not intend to provide instructions for installation. A MR instrument is only to
be installed by Bruker Service or by personnel authorized by Bruker.
1.2 Overview
Chapter Safety [} 9] provides safety instructions for the System Owner (responsible body)
for the installation procedure. There are also references to safety instructions that are not
relevant for carrying out installation itself, but which should be heeded during subsequent
operation and may have repercussions on installation planning. Regulatory standards of the
MR instrument are provided in the Chapter Standards [}21].
Chapter Installation Schedule [} 23] provides instructions on the operational sequence of
the planning and installation and describes the necessary prerequisites and responsibilities of
the System Owner, which must be met, before installation by Bruker Service may start.
Chapter Laboratory infrastructure and interactions [} 27] describes the general and
infrastructure specifications for the installation site within a laboratory. Aspects relating to a
subsequent smooth operating procedure and to interactions with other laboratory facilities
and the building are discussed here.
Chapter Planning Details [}37] provides specific and detailed specifications for the various
installation areas of the MR instrument, e.g. required space, cooling water, and electrical
connection data.
Chapter Seismic safety specifications [} 57] therein provides instructions associated with
installation under specific environmental conditions such as e.g. seismic regions.
Chapter Delivery and Transport [}79] lists the prerequisites and conditions for the delivery
and transport of the MR instrument.
Chapter Checklist in Preparation of the Installation [}89] provides a check list that must be
used for installation planning.
1.3 Additional Documents
The existing manual supplements the documentation of the BioSpec® MR instrument for the
sole purpose of planning the installation.
The installation of a MRI CryoProbe™ or Inline PET requires additional installation planning
and additional installation requirements. Please contact your local Bruker office.

Introduction
8 Site Planning Information BioSpec 70/30 USR_2_V020
1.4 Responsibilities
The responsibility for planning installation and for correctly implementing the installation
requirements lies with the MR System Owner, also if the System Owner has outsourced
planning services to external provider. Unless otherwise stipulated in the individual case by
the contract of sale, Bruker can only provide information to the System Owner.
The transfer of risk is established in the general business terms and/or in the "Terms and
Conditions" of the respective contract of sale and must accordingly be taken into account
when the MR instrument owner plans the installation.
If there is a delay of receipt on the part of the System Owner after readiness for shipment has
been sent to Bruker, the System Owner may be charged for the resulting additional costs.
The regulations on a delay of receipt are established in the General Business Terms and the
"Terms and Conditions" of the respective contract of sale.
1.5 Validity
This information represents the technical status of the installation requirements at the time of
publication.
Technical modifications that are established in the general business conditions and in the
“Terms and Conditions” of the contract of sale may require modifications in the installation
requirements.
Any instrument configurations shown in this manual are typical examples. However, the final
configuration of the ordered instrument is solely defined by the corresponding contract.

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 9
2 Safety
2.1 Safety during the Installation Phase
Unless not subcontracted to third parties, the System Owner (responsible body) is
responsible for the safety during the installation phase. Follow the safety notices below and
plan in advance to have measures to reduce risks already in place prior to the installation
phase. Risks arise for people, buildings, and equipment.
NOTICE
Installation, initial commissioning, retrofitting, repairs, adjustments or dismantling of the
device must only be carried out by Bruker Service or personnel authorized by Bruker.
Damage due to servicing that is not authorized by Bruker is not covered by your warranty.
WARNING
Warning of personal injury and/or damage to the MR Instrument and/or
building.
Transport, storage, and placement of the MR instrument must be carried out with particular
care. Only trained, qualified, and authorized personnel is allowed to handle the MR
instrument. Occupational standards and minimum safety distances between building
structures and heavy loads must be fulfilled along the entire transport route. The load-
bearing capacity of all transport routes, storage facilities, and installation areas of the
equipment components must be checked and released by professional personnel.
If operations are performed by unqualified personnel, the following risks exist:
uPersonal injury when transporting heavy loads
uSignificant damage to the MR instrument due to inappropriate handling
uStructural damage to buildings due to heavy loads
CAUTION
Heavy load.
This service action requires handling of heavy loads.
uEnsure compliance with worker protection regulatory limits.
uPotentially, hire professional staff trained in handling heavy loads.

Safety
10 Site Planning Information BioSpec 70/30 USR_2_V020
WARNING
Danger of injury from electrical shock.
A life threatening shock may result when housings or cabinet doors are opened and covers
or components are removed while connected to the line power.
Only authorized and electrically qualified personnel should carry out work.
uSwitch instrument/component OFF and disconnect from line power.
uPrevent reconnection.
uTest for absence of harmful voltages.
WARNING
Risk of personal injury or death due to effects originating from strong
magnetic fields: Establish and maintain a Controlled Access Area.
Approaching a strong magnetic field bears various risks that can lead to serious injuries. It is
the responsibility of the System Owner (responsible body) to establish and maintain a
Controlled Access Area which is defined by the three dimensional region where the
magnetic field exceeds 0.5 mT (5 Gauss). People with medical implants or pacemakers or
people carrying magnetic parts must not enter the Controlled Access Area.
uEstablish a Controlled Access Area with minimum size of the 0.5 mT region.
uTrain and authorize all personnel that needs to enter the Controlled Access Area. Do
not forget to inform facility management, cleaning personnel, or potentially local fire
brigade.
uCheck authorization regularly and refresh training on safety.
uRefuse and prevent actively people carrying medical implants entering the Controlled
Access Area.
uRefuse and prevent actively non-authorized and or non-trained personnel entering the
Controlled Access Area. Inform visitors on the potential risk originating from the high
magnetic field. Do not leave visitors unattended.
uIt is good practice to establish a standard procedure when entering the Controlled
Access Area and depose all personal accessories outside that might be affected or
become dangerous projectiles.
WARNING
Risk of severe personal injury or explosion
uOnly Bruker Magnet Service may perform work on the magnet.
uDo not open or remove any safety valves and/or burst disks of the magnet dewar.

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 11
WARNING
Risk of asphyxiation or severe burns by cryogen liquids
The handling of cryogen liquids bears potential risk of severe personal injuries like burns or
asphyxiation. Rapidly escaping helium or nitrogen as it occurs during the filling process of a
magnet can possibly displace the air in the magnet room and can therefore be a potential
risk for asphyxiation.
uOnly authorized and trained personnel may refill cryogen liquids.
uAlways have a second person supervising from outside while handling liquid cryogens.
uWear suitable protective gloves and eye protection.
uUse only non-magnetic dewars and carts.
uStore cryogen liquids in sufficiently ventilated rooms only.
uNo humans are permitted inside an elevator (or any other insufficient and closed room)
that hosts dewars with cryogen liquids.

Safety
12 Site Planning Information BioSpec 70/30 USR_2_V020
2.2 Instructions to plan a safe and compliant MR Site
Laboratory infrastructure and operation of the MR instrument must be safe and compliant
with regulatory. The following safety aspects need to be considered when planning the MR
site.
2.2.1 Magnetic Field
The super-conducting magnet of the MR instrument continually produces a very strong
magnetic field inside of and outside of the magnet. The magnetic field outside of the magnet
typically extends much further than the magnet housing to the surrounding area. To minimize
hazards due to unawareness of the influence of strong magnetic fields, access to this area
must be restricted. The System Owner (responsible body) has to establish a Controlled
Access Area.
Figure2.1: Hazardous Zones
1a Controlled Access Area
1b Exposed area
2 Access to the Controlled Access Area
3 Access to the exposed area
4 Gas exhaust discharge area

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 13
2.2.1.1 Controlled Access Area
The Controlled Access Area is the three-dimensional area in which the magnetic field
strength is 0.5 mT and higher. It is defined by the 0.5 mT magnetic stray field line of the
magnet. The contour maps of the magnetic stray field are provided in the System Owner
Manual.
System owner or authorized personnel must ensure that individuals entering the Controlled
Access Area must put down all loose ferromagnetic objects and must be questioned about
their medical history (e. g. with respect to pace-makers, surgical implants, metal splinters,
etc.).
Individuals with implants (e.g. cardiac pacemakers, clips, prostheses, etc.) are not allowed to
enter the Controlled Access Area and pass beyond this 0.5 mT contour line.
It is the responsibility of the system owner to inform all users about applicable national and
local regulations and occupational standards.
WARNING
Risk of personal injury or death due to effects originating from strong
magnetic fields.
Entering the Controlled Access Area bears various risks that can lead to serious injuries.
People with medical implants or pacemakers must not enter the Controlled Access Area.
uRefuse and prevent actively people carrying medical implants entering the Controlled
Access Area.
uRefuse and prevent actively non-authorized and or non-trained personnel entering the
Controlled Access Area. Inform visitors on the potential risk originating from the high
magnetic field. Do not leave visitors unattended.
uBe familiar with the extension of the 0.5 and 3 mT region within the Controlled Access
Area.
uAlways reflect and be aware of the potential risks when entering the Controlled Access
Area. Accidents occur often by being unthoughtful or careless during routine work in the
Controlled Access Area.
uIt is good practice to establish a standard procedure when entering the Controlled
Access Area and depose all personal accessories outside that might be affected or
become dangerous projectiles.
WARNING
Risk of personal injury or death
The magnetic field of the superconducting magnet remains on even when the MR instrument
is powered off.
uAll safety measures associated with the Controlled Access Area have to be observed
even when the MR instrument is powered off.

Safety
14 Site Planning Information BioSpec 70/30 USR_2_V020
NOTICE
Malfunction of laboratory equipment sensitive to magnetic fields
Within the Controlled Access Area, the MR instrument can
1. interfere with electronic devices so that they might not work as intended
2. be influenced by electronic devices so that image quality can be affected
uAlways check accessories located in the Controlled Access Area for MR compatibility.
2.2.1.2 Exposed Area
The exposed area is the three-dimensional area in which the magnetic field strength is
between 0.1 and 0.5 mT. It is defined by the corresponding stray field lines of the particular
magnet types. The exposed area is not a general area of risk.
NOTICE
Malfunction of laboratory equipment sensitive to magnetic fields
Within the Exposed Area, sensitive equipment may still experience impairment of
functionality, disturbance, or defects.
2.2.1.3 Exhaust system
To rule out the risk of asphyxiation or injury during a quench of the magnet (unwanted
discharge of the magnet with high amount of cold Helium gas escaping rapidly), the magnet
can only be installed and energized if a correctly designed and installed exhaust vent system
is in place.
When planning the exhaust vent, attention should be paid to the following:
• The exhaust vent and the discharge area need to fulfill their function permanently.
• The helium gas leaving in the exhaust vent into the discharge area is very cold so that
within an area of about 3 m distance, no access for persons must be possible. The helium
gas itself does not constitute a risk for people, animals, or objects.
• A room ventilation is also required even when the magnet is cold but not energized.
2.2.2 Cryogenic Fluids and Gas
Cryogenic fluids (liquid helium, for some magnets also nitrogen) are used in the magnet to
maintain superconductivity. The handling and storage of these extremely cold fluids requires
compliance with the safety measures described in the chapter Instructions to plan a safe and
compliant MR Site [}12] and may only be carried out by authorized personnel.
If storage facilities for cryogenic fluid containers are foreseen, these rooms must meet the
safety regulations for storing these gases. Attention must be paid when planning these rooms
to ensure that:
• The room's ventilation system guarantees a fresh air exchange rate of at least 1.5 times
the rooms volume per hour
• The proper function of the ventilation system is monitored continuously and appropriate
safety precautions are taken to indicate a failure of the ventilation system
• Escape routes cannot be blocked by situations in which overpressure may occur

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 15
WARNING
Blocking of escape routes from overpressure
Escape routes and emergency exits must be designed such that they work reliably under
overpressure conditions.
WARNING
Failure of ventilation system
The correct function of the ventilation system in the magnet room and in cryogenic fluid
container transport and storage locations is a general prerequisite for the safe handling of
cryogen fluids. Therefore, the ventilation system needs to be maintained and its operation
monitored continuously.
2.2.3 Oxygen Supervision
The correct function of the ventilation system in the magnet room and in cryogenic fluid
container transport and storage locations is a general prerequisite for the safe handling of
cryogen fluids. Therefore, the ventilation system needs to be maintained and its operation
monitored continuously
In order to establish a second safety level, most important for magnets that contain also N2,
Bruker BioSpin MRI recommends the installation of an oxygen (O2) supervision and alarm
system in the magnet room and in storage locations for cryogen fluid containers.
An O2 supervision unit is not part of the delivery of the MR instrument. It is the responsibility
of the System Owner to decide on the usage of O2 supervision and to install and maintain it.
MR compatible O2 supervising units can be obtained from various commercial providers.
2.2.4 Emergency Plan
The conduct of staff and external parties in case of emergency must be included in the
installation planning and must be communicated. Appropriate emergency access to the
Control Acess Area must be tolerated. Therefore, the following organizations must be trained
accordingly:
• Building administrators
• Fire department
• Police
• Emergency paramedics
• Building security
Attention must be paid in planning the facility to ensure that non-magnetic emergency
equipment is available, or that it is possible to use the equipment safely within the Control
Access Area:
• Provision & labeling of non-magnetic fire extinguishers
• Use of emergency lighting suitable for magnetic fields (see Chapter Lighting system
[}69])
• Prevention of use of ferromagnetic oxygen cylinders by emergency personnel

Safety
16 Site Planning Information BioSpec 70/30 USR_2_V020
• Clarify the use of defibrillators in the Controlled Access Area.
In addition, consider that in case of emergency other exits than those intended may be used
in panic. Therefore, design
• Exits and emergency routes to work during over- or underpressure conditions.
• Label potential areas and routes that may also be used and avoid inappropriate escape
routes.
2.2.5 Fire Prevention
The MR instrument does not require any dedicated fire prevention. If fire prevention
apparatus is to be used at the installation site, the following notices should be heeded when
planning installation:
• Many smoke detectors react to escaping helium gas. Already small amounts which may
be released during maintenance work on the magnet may trigger a false alarm. To avoid
inadvertent false alarms, detectors that are heat triggered should be used instead.
• Heat detectors and sprinklers often require access for regular maintenance work.
Attention should be paid to ensure that these access points are not located in the area
above the magnet, since maintenance is not possible there while the magnet is on field.
• To prevent damage to the magnet and to the MR instrument’s control electronics due to
false alarms, Bruker BioSpin MRI recommends to use so-called pre-action sprinklers.
2.2.6 Biological Safety
In relation to the health and safety of employees and laboratory animals, we refer to
compliance with national standards and regulations.
Repairs by Bruker BioSpin MRI can only be carried out after decontamination of equipment
has been demonstrated in writing and any damage to the health of Bruker BioSpin MRI
employees has been ruled out.
Appropriate aids, access, and procedures that rule out any damage to the health of service
personnel must therefore be provided for maintenance, service, and repair of the MR
instrument.
• Measures and aids for cleaning and decontaminating hardware and accessories.
• Depending on biosafety level, site planning needs potentially consider a physical
separation between hazard areas and other parts of the MR instrument, e.g. the technical
room where separate access through a non-hazardous area may be reached.
2.2.7 Seismic Safety
Installations in areas at seismic risk require additional planning and potentially MR instrument
modifications to be compliant with safety requirements. Siting and building construction
constraints require early individual planning. Provisions are not within the scope of the MR
instrument’s standard delivery. See chapter Seismic safety standards [} 57] and contact
your local Bruker BioSpin MRI planning office.
2.2.8 Safety at Work
Safety at work is in the responsibility of the System Owner and needs to be in compliance
with the national regulations. Additional aspects arising from the installation of the MR
instrument are typically:
• Exposure time to static and electromagnetic fields.

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 17
• High noise levels originating from the magnet room and the technical room.
• Ventilation in the magnet room, see Ventilation and air conditioning [}69], Oxygen
Supervision [}15].
• Extraction of anesthetic gases where applicable, see Anesthetic gas extraction [}71].
Appropriate countermeasures are to be considered in an early planning phase.
See also
2USER WORK SAFETY DS - Isoflurane - Rev0 [}18]

Isoflurane Anesthesia Gas
1-Chloro-2,2,2-Trifluoroethyl Difluoromethyl Ether; 2-Chloro-2-(Difluoromethoxy)-1,1,1-trifluoro Ethane; Forane
The substance/gas, ISOFLURANE, carries the CAS-no. 26675-46-7, regulated by the European REACH
regulation 1907/2006. Isoflurane, a halogenated anesthetic gas, is a potential health hazard and
safety procedures should be followed before/during its use to reduce risks (see MSDS of supplier)!
General Information:
EC#
:
247-897-7
CAS#
:
26675-46-7
OSHA IMIS #
:
F118
ICSC #
:
1435
RTECS #
:
KN6799000
Molecular Formula
:
C3H2ClF5O
for Europe:
Within all EU member states, the European Work Safety Regulations must be followed:
-see: The European Commission Website –Health, Hygiene, Safety at work
WAG - Waste Anesthetic Gas: extract from: “UGA IACUC Isoflurane Safety Guidelines“/ 2011
Isoflurane is commonly used to anesthetize research animals. While safer than previous generations of halogenated
anesthetic agents, efforts must be made to limit exposure risks to the user.
People working with Isoflurane and other volatile anesthetics can be exposed to waste anesthetic gas (WAG),
especially in certain situations frequently encountered when anesthetizing rodents:
1. When using a nose cone/face mask that does not form a tight seal around the animal’s face.
WAG can leak around the mask into the room!
2. When using an induction chamber. Opening the charged chamber to retrieve the induced
animal releases WAG into the room. Sliding induction chambers are safer than hinged.
3. When using an open system (bell jar, conical tube) rather than a vaporizer with scavenging.
4. When using a sterotaxic surgery device. WAG is released below the animal’s head.
5. When a non-rebreathing system is not used, which allows more waste gas to be released
from the supply tubing.
Signs of acute exposure: nausea, vomiting, nose/throat/respiratory irritation, headache,
dizziness, drowsiness, skin irritation
Signs of chronic exposure: hypotension (low blood pressure), tachycardia (increased heart
rate), respiratory depression, elevated blood glucose.
OSHA: OSHA has not established a permissible exposure limit (PEL) yet, but it is recommended
that no worker should be exposed to greater than 2ppm of any halogenated anesthetic
agent, such as isoflurane. Some European countries already have set PEL/MAK values!
Summary: Work Safety requirements towards Engineering Controls demand the following:
To take care before initial use:Isoflurane must only be used in a well-ventilated
room from which there is no recirculation of exhaust air !
USER WORK SAFETY Data Sheet

Safety
Site Planning Information BioSpec 70/30 USR_2_V020 19
2.2.9 Infrastructure Supervision and Messaging
In order to increase reliability and safety a supervision network may be considered that is
interfaced to a central alarm network or messaging system. Consider:
• Smoke detectors.
• Access supervision of restricted areas.
• Process water supply for cooling system.
• Magnet room ventilation system (must).
• O2 supervision.
• Magnet status supervision via the magnet monitoring unit interlock signal (see technical
room)
It is in the responsibility of the System Owner to decide and install appropriate measures
such as
• acoustic and/or visual alarm signs within the MR and laboratory rooms.
• suitable alarm signals to a central supervision or alarm network of the building.
• automatic messaging for example via email or phone message to the supervising
headquarter and/or staff in charge.
Bruker BioSpin MRI is not responsible for the connection to the supervision network or
messaging system and not liable for failures or any possible damage caused when
connecting the MR instrument to this system.
Suitable alarm notifying pathways and procedures must be established that also take into
account absence of laboratory staff during week-end or vacation periods.

Safety
20 Site Planning Information BioSpec 70/30 USR_2_V020