Cellink Inkredible+ User manual

User Manual
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“You cannot create new science unless you
realize where the old science leaves off and
new science begins, and science- fiction
forces us to confront this.”
Michio Kaku

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Table of Contents
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1
INTRODUCTION OF
YOUR INKREDIBLE
3D BIOPRINTER

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INTRODUCTION OF THE INKREDIBLE
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Hello and welcome to the exciting world of 3D Bioprinting. The
technology you have in front of you has the potential of creating
real, living human life and it is only your imagination that limits
what you can do. This manual will serve as your guide for setting
up your INKREDIBLE bioprinter, maintain it, and also notify you
about safety precautions. Let`s begin this exciting journey!
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WARNING: The INKREDIBLE includes moving parts that can cause injury. Never
reach inside the INKREDIBLE while it is in operation. Always allow the
INKREDIBLE to come to a complete stop prior to reaching inside.
WARNING: Do not leave the INKREDIBLE unattended during operation.
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SPECIFICATIONS
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PRINTING
Print Technology: Extrusion based bioprinting
Build Volume: 130 x 80 x 50 mm
High 100 microns [0.0039 in]
Standard 200 microns [0.0078 in] Low 300 microns [0.0118 in]
Positioning Precision: XY: 11 microns [0.0004 in]; Z: 2.5 microns [0.0001 in]
Needle Diameter: User dependent
Needle Height: User dependent
SOFTWARE
Software Bundle: Repetier Host
File Types: .stl, .obj, .thing
Supports: Windows (XP 32 bit/7+)
Ubuntu Linux (12.04+)
Mac OS X (10.6 64 bit/10.7+)
PHYSICAL DIMENSIONS
19.1 x 16.5 x 14.7 in [330 x 370 x 300 mm]
Shipping Box: 23 x 21.5 x 17 in [59 x 55 x 43 cm]
Weight: 25.4 lbs [11.5 kg]
Shipping Weight: 44.55 lbs [20.2 kg]
ELECTRICAL
AC Input: 100 – 240 V, ~2 amps, 50 – 60 Hz
Power Requirements: 24 V DC @ 6.25 amps
Connectivity: USB, SD card [FAT 16, max. 2GB]
MECHANICAL
Body: Powder-coated high grade steel
Build Platform: Acrylic
XYZ Bearings: Wear-resistant, oil-infused steel
Stepper Motors: 1.8° step angle with 1/16 micro-stepping

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HOW DOES IT WORK?
The INKREDIBLE bioprinter is an extrusion based 3D Bioprinter with dual extruder
heads for bioprinting of human tissue models and organs. It is a cost-effective unit to
enter the 3D bioprinting field and for innovators and beginners to start bioprinting living
tissues at ease. It can be used as a standalone unit thanks to its LCD display or it can
be monitored through a computer with the use of the accompanied software. Your
three-dimensional design files are translated into coordinates and instructions for the
INKREDIBLE and allows the bioprinter to move according to a defined plan. The
printing process works through the extrusion of a bioink or hydrogel material combined
with the extrusion of human cells layer by layer until a 3D structure is built from bottom
to top. Once the structure has been printed, it can be cross-linked using the Blue LED
UV crosslinker or ionic solutions, depending on your bioink material.

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2
UNPACKING YOUR
INKREDIBLE 3D
BIOPRINTER
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UNPACKING YOUR INKREDIBLE 3D BIOPRINTER
When unpacking your INKREDIBLE 3D Bioprinter, be careful not to grab onto any
movable parts or lose components. We recommend you grab it by the top window frame
and pull it out from the box.
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NOTE: Do not force or tear anything during the unpacking or setup process as
this may irreversibly damage your INKREDIBLE 3D Bioprinter
CAUTION: When opening the box, do not force or tear anything while
unpacking as this may damage the INKREDIBLE 3D Bioprinter.

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CONTENT OF THE BOX
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[1x] INKREDIBLE 3D Bioprinter
[1x] 24V Power supply
[1x] Power cord
[1x] SD card pre-loaded with sample objects to print
[1x] USB Cable
[1x] Portable oil-free air compressor with tubing
[1x] STARTINK-Kit (1 prefilled Syringe with 3mL of CELLINK + 1 CELLMIXER + 10mL
of Crosslinking solution)
[3x] Empty cartridges
[1x] 24 well plate
[1x] Petri-dish
Note: Unpacking should be done outside the laboratory environment.