Centurion Scientific PrO-Hospital Ambient User manual

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Instruction Manual
Ambient

IMPORTANT Read before using
Location
The Centrifuge should only be operated indoors.
The set up location must fulfil the following requirements
• Check the Centrifuge and the Packaging for any shipping damage.
Inform the shipping company and Centurion Scientific immediately
•Check the order for completeness, if not contact Centurion Scientific
• A safety zone of at least 30Cm must be maintained around the Centrifuge
• People and hazardous substances must be kept out of this zone
during centrifugation
• The bench or supporting structure must be stable and free from resonance
• The Bench or supporting structure must be suitable for horizontal use of the
centrifuge
•The Centrifuge should Not be exposed to heat or strong sunlight
•Ultra violet rays can damage the stability of plastics and coatings
• Do not subject the Polypropylene, Delrin rotors or accessories to direct
sunlight
•The Zone location must be well ventilated at all times
Transporting the Centrifuge
•Always remove the Rotor before transportation
•Due to its weight the Centrifuge should be carried by several people
•Transport the Centrifuge upright and if possible in its packaging
•Always lift the centrifuge at both sides (see below). NOT front to rear.
• Store the original packaging, Keep this for future service to base , if required.
2

Fixing of rotor
Fixing for Swing out rotors.
BRK5508, BRK5506, BRK1001, BRK3000, BRK5540, BRK5740.
Locate 3 pins of fixing, into 3 holes at the top of the rotor
Push down firmly into holes.
Lift and fit rotor onto the motor shaft. M
Fit the Screw and spring washer, screw down tight with
the supplied tool.
Check tightness regularly.
Fixing for micro and fixed angle rotors
BRK5401. BRK54XXseries. BRK53XX series. BRK52XX series
Lift and fit rotor onto the motor shaft.M
Fit the Screw and spring washer, screw down tight with the
supplied tool.
Check tightness regularly
Motor shaft
M
3

Rotor balancing. Very Important
Swing out rotor, buckets. Balancing
Balancing of tubes for Micro and Fixed angle rotors
Tare (zero) your scales first
Weigh a tube , note its
weight Weigh another and
match its weight to within 0.1
gram
Place all your required tubes in all 4 buckets.
Equal amount per bucket.
Weigh each bucket containing tubes
Match other 3 buckets, with tubes to within 1
gram of each other
4
For BRK5506 rotor use the adaptor

Tube placement micro rotors
Haematocrit Rotor
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram. For best separations. (see page 4)
ALWAYS fit the lid supplied
CHECK the Rim seal gasket and replace every 2 months (Part number 8011)
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
Micro tube Rotor
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram For best separations. (seep page 4)
ALWAYS fit the lid supplied
CHECK the two top seals and replace regularly (Part number 8044)
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
Always fit the lid supplied, as shown to the left
Always fit the lid supplied, as shown to the left
...........................................................................................................................................................................
5

Tube placement Fixed angle rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly
✔ ✘ ✘
Large fixed angle Rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly
✔
✔ ✘
✘
✘
✘
...................................................................................................................................................................................
Small fixed angle Rotors
Always fit the lid supplied, as shown to the left
Always fit the lid supplied, as shown to the left
6

Tube & bucket placements Swing out rotors
Medium – Large Swing out Rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations
ALWAYS fit ALL the Tube Holders and any supports
CHECK The rotor screw fixing tightness regularly
ALWAYS load your tubes opposite to each other
ALWAYS Weigh Bucket and all samples within to 1.0 Gram
ALWAYS fit ALL 4 Buckets and 4 Adaptors supplied
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
✔ ✘ ✘
✘
Microtitor Rotor
ALWAYS load your plates opposite to each other
ALWAYS weigh samples to within 1.0 Grams. For best separations
ALWAYS fit both Buckets supplied (2)
CHECK the rotor screw fixing tightness regularly
✔
.................................................................................................................................................................................
.................................................................................................................................................................................
Small Swing out rotor
7

This is not balanced!
Even though there are two samples in two buckets opposite to each other it is not balanced.
NEVER run swing out rotors with only two buckets
All four buckets must beusedat alltimes
Wrong
Wrong
Tube placements Swing out rotor
Always use the same size tubes as in A
per bucket.
NEVER use different size tubes as in B
per bucket
Check they fit and do not hit the
centre as shown in C
A B
C
8

Tube placement Cytology & Cell wash rotors
Cytology Rotors
Manual Cell W ashing Rotor
ALWAYS load your holders opposite to each other
ALWAYS weigh samples to within 0.5 Gram. For best separations
ALWAYS fit the lid supplied
Check the two top Gaskets and replace regularly (Part Number 8055)
CHECK the rotor screw fixing tightness regularly
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.2 Gram. For best separations Regularly smear a small amount of Silicon grease over the O rings
Check the inner and outer O rings and replace regularly (Part Number 8066)
CHECK the rotor screw fixing tightness regularly
✔
✔ ✘
✘
✘
✘
................................................................................................................................................................................
Always fit the lid supplied, as shown below
9

Prime and Dual models only. Ambient
Rotor RCF O Speed x 10 Time (Mins)
ACCELL DECELL O RPM Memory
Accel Decell Memory Memory
Store Function Rcf/Rpm Recall
START POINT.
A. Selection of your fitted Rotor
First set the rotor 0-99. See the rotor option relevant to your Centrifuge.
Rotor Speed x 10
Rotor part number will appear here in
the large display. (Example shown)
Press the arrows to select the desired Rotor.
NOTE. Dual models have 1-2 selection only. Model dependant
Start
Stop
Lid
Open
Pulse
(Sec)
1 0
5 4 0 1
Instructions. Ambient model
12

B. Setting of Speed or Rcf(G) and acceleration or Decelleration (braking)
RCF O Speed x 10
Speed shown (Example).
The speed shown is x 10 so is 4000 Rpm
ACCELL DECELL O RPM
Accel Decell
Function Rcf/Rpm
1Select if you require Speed or Rcf (G)
RCF (G) is the actual force that does the work for your analysis.
It is speed x the radius maximum of the Rotor that is the separation for analysis.
Press Function button and press the Rcf/Rpm it will pulse dot Left top Rcf and Bottom right RPM
2 Select the value you require by using the or Arrows
In increments of 10 Rpm or 10 Rcf(G)
3Select your acceleration rate (9 is fastest 0 has no braking and is slowest)
Press Function and Accel increment from 0-9
4Select your Decelleration rate (9 is fastest 0 is slowest)
Press Function and Decell increment from 0-9
............................................................................................................................................................................
C. Selection of Time period Time (Mins)
Select Time by pressing upor down arrow.
Time shown is 5 minutes (Example)
Memory
Memory Memory
For “Pelleting” or fast separations up to 60 seconds
Use the Pulse button, when held down the timer counts up in seconds
Once your desired time is reached, release the button
Recall
0 4 0 0.
Lid
Open
0 5
Pulse
(Sec)
Time periods run 0-99 minutes.2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h & CO
2h, etc means 2 hours and so on, CO means constant
Instructions. Ambient model
13

D. Programming, 100 are available from 0 – 99
Set all parameters as previous details. A Rotor, B . Speed or Rcf(G), Accel, Decell. C Time
Memory
Memory Memory
Store Function Recall
After setting all your required parameters.
Press Function and select memory to your desired number by pressing Memory buttons
Once done whilst pressing Function Press Store.
To recall a program Press Function and memory to desired program number
Once done whilst pressing Function Press Recall.
NOTE. The last run data (program must be run) will be kept, after turning off the Centrifuge power
9 9
Instructions. Ambient model
Speed will not go beyond 2000 Rpm.
.
.
.
.
Noisy
.
.
.
The Electronics have set the Speed to safe mode
Check the rotor bottom has magnets fitted
Check correct rotor is in use against program chosen
If not then Contact our Service Department
Unbalanced tubes, see Rotor pages
Lid seal needs replacing
Rotor Lid not fitted
Lid support fail
. Spring has failed. Contact our Service Department
Simple problems
14

Fault codes are shown in the Speed section of the LED display
The following List show what each Code means and what to do.
F A 1 0
F A 2 1
F A 2 2
F A 2 3
F A 2 4
F A 2 5
F A 2 6
F A 2 7
F A 28
F A 3 0
F A 4 0
F A 50
F A 51
No rotation seen
Fault codes. Ambient model
Check the Following with the Lid open.
Tubes are too tall and foul the Lid, replace with correct ones
Check the rotor to see if it freely turns, if not look for
Objects in the bowl that may be fouling and remove.
If not Contact our Service Department
Check the Following.
Weigh your tubes to 0.1g of each other
Place tubes in Rotor opposite each other
(see previous Rotor pages for instructions)
If Fault code persists call our Service Department.
As above.
As above.
As above.
Contact our Service Department.
Contact our Service Department.
Contact our Service Department.
See the following Rotor pages
Close the Lid, press down on 2 front corners, push firmly. If
problem persists Contact our Service Department
Wrong rotor fitted or missing magnets if so.
Contact our Service Department
Contact our Service Department. Also See below
Contact our Service Department. Also see below
Look at the Obround viewing port (Rear)
For FA 50 and FA 51
Note the Code shown in the inverter display. *
Turn off the power at the wall mains switch only.
WAIT 3 minutes, Turn on again and Run. If problem persists
Contact our Service Department, with the Inverter code
As above.
*
*
Obround viewing port shown. Situated
at the rear of the Centrifuge
Imbalance BG
Imbalance BD
Imbalance MG
Imbalance MD
Imbalance base sensor
Imbalance Motor sensor
Data time out
Imbalance
Lid is open
Rotor recognition
Drive
Druc
15

Emergency Lid release
Mechanical Emergency Door release
During a power failure, you will not be able to open the Centrifuge lid via the Display button
Mechanical override is provided to allow sample recovery
However this is for Emergency use only
This should Not be used for normal use.
Warning
Therotorcanstillbespinningatahighspeed,iftoucheditcancauseseriousinjuries.
Alwayswaittilltherotorhasstopped.
Proceedasfollows
•Make sure the Rotor has stopped (view via the port in the Lid)
•Once stopped turn off the power via the inlet Switch
•Pull the Centrifuge slightly over the bench (as shown in (a)
•You will notice one (b) or some models two plastic plug (c). Plug *
•Pop open with a screwdriver (tool) and pull downwards .
•The lid will pop open You can now retrieve your samples
AfterreplacethePlasticplugsbackintotheiroriginalposition.
Reconnectthecentrifugepowerlineoncethemainspowerhasbeenrestored.
Checkallisworkingcorrectlybyclosingthelid,wait5secondsthenpresslidopen.
If not working refit or Contact your Distributor
a
b
cc
16

Nomograph. For Rcf evaluation.
NOTE centrifuges can be programmed in Rcf(G)
Example
To find the relative centrifugal force
at a radial distance of 10 cm from
the center of rotation when
operating the centrifuge at a speed
of 3000 r.p.m., place a straightedge
on the chart connecting the 10cm
point on the Rotating Radius Scale
(A) with the 3000 r.p.m. point on
the Speed Scale (B).
Read the point at which the
straightedge intersects the Relative
Centrifugal Force Scale (C) – in this
case, 1000 x gravity.
Similarly, if the desired “r.c.f.” is
known, the necessary speed for a
given rotating radius may be
determined by connecting the two
known points and reading the
intersection of the straightedge with
the Speed Scale.
Equation for Calculating R.C.F.
R.C.F. = .00001118 x r x N2
R.C.F. = relative centrifugal force (gravities)
r = radius from center of rotation to bottom of tube (cm)
N = rotating speed (rev. per minute)
17

Care & maintenance. Centrifuge
Always keep your Centrifuge clean and free from moisture at all
times, this will help prevent corrosion.
It is recommended that your centrifuge is switched off and the lid left
open overnight to ensure that any moisture build up can evaporate.
Always ensure that lids and accessories are correctly tightened
before running the unit.
Keep a 30Cm distance around your Centrifuge.
Do not site the unit in sunlight
Do not use any kind of scourers or abrasive material to clean the
Centrifuge and ensure that a pH neutral cleaner is always used.
Use only official Centurion Scientific Ltd accessories with your centrifuge.
It is recommended that regular maintenance and cleaning of the unit and
accessories is carried out.
!!
!
!
!
!
!
!
!Yearly servicing is required to validate your warranty and increase the life
of your Centrifuge and accessories. See PrO-Care pages
10

Care & maintenance. Rotor
Always check rotors for cracks and damage, if found replace
your rotor. DO NOT USE as dangerous
Autoclave for the maximum number of times shown in the
sales literature. In brackets( 20)
Rotors have a Seven year lifespan and must be replaced .
DO NOT USE beyond their Lifespan
Ensure that all buckets are present in the rotor when running your
centrifuge, as using the unit with an unbalanced rotor can cause distortion
and excessive wear. Read tube placements section in the previous section
Do not use any kind of scourers or abrasive material to clean the buckets
and ensure that a pH neutral cleaner is always used.
Aluminium buckets should be kept clean and moisture free to keep them in a
good, safe operating condition.
It is recommended that regular maintenance and cleaning of the rotor and
accessories is carried out.
!!
!
!
!
!
!
!
!Yearly servicing is required to validate your warranty and increase the life
of your rotor and accessories
11

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Company Representative
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The Old Stables, Church Farm, Stoughton, Chichester, West Sussex. PO18 9JL. United Kingdom.
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Centurion Scientific Ltd of The Old Stables, Church
Farm, Stoughton, Chichester, West Sussex, PO18 9JL,
UNITED KINGDOM
+ereby confirm that all centrifuges and accessories
supplied by us are of UNITED KINGDOM origin.
Signed for and on behalf of Centurion Scientific Ltd
Granted 20RQZDUGV
Company Representative
The Old Stables, Church Farm, Stoughton, Chichester, West Sussex. PO18 9JL. United Kingdom.
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CERTIFICATE
OF 25,*,1
Scientific Limited
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Research & service
The Old equine Barn
Church Farm
Stoughton Chichester
PO18 9JL. UK
Ambient
manufacturing
The Old Grain Barn
Church Farm
Stoughton Chichester
PO18 9JL. UK
Refrigeration
Manufacturing
The Old Drier Barn
Church Farm
Stoughton
Chichester
PO18 9JL. UK
Sales
The Old Stables Church
Farm Stoughton
Chichester
www.Centurionscientificglobal.com Switchboard. 0044 (0) 2392 631225
The lifespan of a centrifuge depends
upon how well it is looked after and
maintained.
Many of our centrifuges have lived a life
of over 15 years.
This is not only due to our stringent manufacturing process
and high quality components but also to a yearly servicing
package. Making them a cost effective solution of centrifugation.
Whilst our centrifuges come with a confidence giving
warranty, yearly services are imperative to ensure your warranty
is valid, this also ensures your machine is working to
its best ability and lengthens its life span. Also as centrifuges
are a dangerous machine, safety checks are required too.
For UK customers we offer a back to base service package
including carriage to us and return all within 48 hours. Centrifuge
seals , fuses & other parts are replaced,
adjustments made, rotor checked for stress cracks, seals changed
if necessary (extra charge) PAT (portable appliance test) is
included Obligatory in UK.
For worldwide customers we offer a full parts package per model.
The designated country distributor services the units accordingly.
Call our Office to discuss or visit our Service website
WWW.ServicePrO-Care.com
PrO-Care
Service, Warranty & Maintenance pakages