Centurion Scientific Pro-Research User manual

Centurion
Scientific Limited
LCD display centrifuges
PrO-Research
Ambient & Refrigerated instruction manual

IMPORTANT Read before using
Location
The Centrifuge should only be operated indoors.
The set up location must fulfil the following requirements
• Check the Centrifuge and the Packaging for any shipping damage.
Inform the shipping company and Centurion Scientific immediately
•Check the order for completeness, if not contact Centurion Scientific
• A safety zone of at least 30Cm must be maintained around the Centrifuge
• People and hazardous substances must be kept out of this zone
during centrifugation
• The bench or supporting structure must be stable and free from resonance
• The Bench or supporting structure must be suitable for horizontal use of the
centrifuge
•The Centrifuge should Not be exposed to heat or strong sunlight
•Ultra violet rays can damage the stability of plastics and coatings
• Do not subject the Polypropylene, Delrin rotors or accessories to direct
sunlight
•The Zone location must be well ventilated at all times
Transporting the Centrifuge
•Always remove the Rotor before transportation
•Due to its weight the Centrifuge should be carried by several people
•Transport the Centrifuge upright and if possible in its packaging
•Always lift the centrifuge at both sides (see below). NOT front to rear.
• Store the original packaging, Keep this for future service to base , if required.
2

Fixing of rotor
Fixing for Swing out rotors.
BRK5508, BRK5506, BRK1001, BRK3000, BRK5540, BRK5740.
Locate 3 pins of fixing, into 3 holes at the top of the rotor
Push down firmly into holes.
Lift and fit rotor onto the motor shaft. M
Fit the Screw and spring washer, screw down tight with
the supplied tool.
Check tightness regularly.
Fixing for micro and fixed angle rotors
BRK5401. BRK54XXseries. BRK53XX series. BRK52XX series
Lift and fit rotor onto the motor shaft.M
Fit the Screw and spring washer, screw down tight with the
supplied tool.
Check tightness regularly
Motor shaft
M
3

Rotor balancing. Very Important
Swing out rotor, buckets. Balancing
Balancing of tubes for Micro and Fixed angle rotors
Tare (zero) your scales first
Weigh a tube , note its
weight Weigh another and
match its weight to within 0.1
gram
Place all your required tubes in all 4 buckets.
Equal amount per bucket.
Weigh each bucket containing tubes
Match other 3 buckets, with tubes to within 1
gram of each other
4
For BRK5506 rotor use the adaptor

Tube placement micro rotors
Haematocrit Rotor
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram. For best separations. (see page 4)
ALWAYS fit the lid supplied
CHECK the Rim seal gasket and replace every 2 months (Part number 8011)
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
Micro tube Rotor
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within0.1 Gram For best separations. (seep page 4)
ALWAYS fit the lid supplied
CHECK the two top seals and replace regularly (Part number 8044)
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
Always fit the lid supplied, as shown to the left
Always fit the lid supplied, as shown to the left
...........................................................................................................................................................................
5

Tube placement Fixed angle rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly
✔ ✘ ✘
Large fixed angle Rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations. (see page 4)
CHECK The rotor screw fixing tightness regularly
✔
✔ ✘
✘
✘
✘
...................................................................................................................................................................................
Small fixed angle Rotors
Always fit the lid supplied, as shown to the left
Always fit the lid supplied, as shown to the left
6

Tube & bucket placements Swing out rotors
Medium – Large Swing out Rotors
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.1Gram. For best separations
ALWAYS fit ALL the Tube Holders and any supports
CHECK The rotor screw fixing tightness regularly
ALWAYS load your tubes opposite to each other
ALWAYS Weigh Bucket and all samples within to 1.0 Gram
ALWAYS fit ALL 4 Buckets and 4 Adaptors supplied
CHECK the rotor screw fixing tightness regularly
✔ ✘ ✘
✔ ✘ ✘
✘
Microtitor Rotor
ALWAYS load your plates opposite to each other
ALWAYS weigh samples to within 1.0 Grams. For best separations
ALWAYS fit both Buckets supplied (2)
CHECK the rotor screw fixing tightness regularly
✔
.................................................................................................................................................................................
.................................................................................................................................................................................
Small Swing out rotor
7

This is not balanced!
Even though there are two samples in two buckets opposite to each other it is not balanced.
NEVER run swing out rotors with only two buckets
All four buckets must beusedat alltimes
Wrong
Wrong
Tube placements Swing out rotor
Always use the same size tubes as in A
per bucket.
NEVER use different size tubes as in B
per bucket
Check they fit and do not hit the
centre as shown in C
A B
C
8

Tube placement Cytology & Cell wash rotors
Cytology Rotors
Manual Cell W ashing Rotor
ALWAYS load your holders opposite to each other
ALWAYS weigh samples to within 0.5 Gram. For best separations
ALWAYS fit the lid supplied
Check the two top Gaskets and replace regularly (Part Number 8055)
CHECK the rotor screw fixing tightness regularly
ALWAYS load your tubes opposite to each other
ALWAYS weigh samples to within 0.2 Gram. For best separations Regularly smear a small amount of Silicon grease over the O rings
Check the inner and outer O rings and replace regularly (Part Number 8066)
CHECK the rotor screw fixing tightness regularly
✔
✔ ✘
✘
✘
✘
................................................................................................................................................................................
Always fit the lid supplied, as shown below
9

Deceleration
Rate Set
Program
Last time
countdown
Open Lid
Last running RCF
Last RCFt
Start
Time counting
down
RCFt counting up
RCF actual
User name
Time Set
RCFt
Not set
RPM or
RCF Set
Programmed
Rotor Set Acceleration
Rate Set
Programmed
Rotor Set Acceleration
Rate Set
Deceleration
Rate Set
Time Set
RCFt
Not set
RCF(g)
Set
Stop
User name
Actual
Temperature
(Refrigerated only)
Temp Set
(Refrigerated only) Temp Set
(Refrigerated only)
Actual
Temperature
(Refrigerated only)
Pre-cool /pre-run
(Refrigerated only)
GENERAL SAFETY
To ensure that the centrifuge is operated in a safe manner, avoiding
the hazard of injury to personnel or damage to material goods, the
following safety precautions should be followed at all times:
•The centrifuge must only be opened by trained personnel competent in its use
• Never use the centrifuge unless the rotor is properly mounted and secure
• Never open, or attempt to open the lid while the centrifuge rotor is still turning.
• Only use original parts for the centrifuge
• Never operate the centrifuge if components are missing or damaged.
• If the rotor shows visible signs of wear its use must be discontinued and replacement fitted.
• The rotor must not be over loaded, the maximum allowed density of samples at full operating
speed is 1.2g/cm3.
• Always endeavour to make sure that opposite tubes are of the same weight to avoid rotor
imbalance. If the tubes are filled with the same material they must, therefore, be filled with the
same amount.
• No changes should be made to the mechanical or electrical components without prior
consultation with and the written permission of Centurion Scientific.
• The centrifuge’s has not been manufactured from inert materials or to be explosion proof.
Ensure that it is not operated within an environment, or with materials that makes these a
requirement.
• During operation a “safe” zone of 30cm must be maintained around the centrifuge. This zone
should be clear of personnel and hazardous materials at all times during the run.
• Substances of a radioactive, flammable or explosive nature must not be centrifuged.
• Substances prone to react briskly with each other must not be centrifuged at the same time.
• Toxins and pathogenic micro-organisms must not be centrifuged unless the proper precautions
for their handling have been taken. These may include, but are not limited to, biological seals.
Should toxins or pathogens enter the centrifuge or its parts the proper procedures for disinfecting
the centrifuge should be carried out.
• Strongly corrosive substances that may cause damage to or impair the mechanical strength of
the rotor may only be centrifuged inside protective vessels. Should any doubt exist regarding the
substance or the suitability of a particular vessel for use with it, consult the Safety Inspector.

Display shown at turn on
Display shown when program key pressed
Display shown when Page key pressed
total of 108 programs can be stored)
Display shown after pressing Tick
on name settings
Display shown after pressing Set
Parameters
Display shown after pressing Rotor
rectangle
Display shown when Set Name pressed
Display shown when chosen name is
being entered
(book emblem top right)
Up to 14 characters, letters &
numbers (we have chosen Biochem8
for these illustrations)

Display shown when Time Numerical
rectangle is selected
Display shown after selecting rotor
- Turn ON Shows white square in centre
Display shown Display shown when timer rectangle
key pressed
Display shown Display shown when RPM pressed
Display shown after selecting RPM
SELECT and PRESS desired timer type:
Time 0 - 9999 minutes
RCFt See W2t document for
explanation
Pulse Timer counts up in seconds
when START button held
down. (90 seconds max)
Hold Timer is held on till stop
button is pressed
Hold and Pulse cannot be changed
Or select RCFt number from
previous
run

Display shown after pressing numerical
rectangle
Display shown when Acc pressed
Display after selecting Acc
Display
Display
Display shown when Dec pressed Display after selecting Acc and Dec rates
then return to set Acceleration and
Deceleration rates below
(bottom right)

Centurion Scientific refrigerated centrifuges have a range of
We accurately control this temperature by running the refrigeration unit and pulsing heat via a loop close to the
coils.
PID system is used to control.
Imagine a bath at home, to control the temperature accurately you would run both hot and cold taps together.
This is far more effective than filling with cold water and topping up with hot as the temperature difference
would be enormous.
By using this method our chamber temperature control is unsurpassed.
Note Due to the efficient management of temperature control you may see some ice patches or in turn, notice
some slightly hotter areas. This is quite normal and it is the overall chamber temperature that matters.
For accurate temperature control we recommend you use the Pre-cool /pre-run facility.
Fit your desired rotor and securely fasten as per our Instructions.
After programming your desired parameters, IE selection of a rotor, speed, time, acceleration & deceleration
rates, (as an example). Leave the centrifuge to reach the desired temperature and then press the Pre-cool
symbol. This will run the centrifuge at 20% of the maximum speed of the rotor for 20 minutes, a will show in
the Rpm section to show the Pre-cool mode is in operation. It is advisable to let the time run to 20 minutes, but
you can stop at any time. Once stopped load your samples (which should be at your set temperature) and
press the start arrow button.
For ease it is advisable to set your samples to the desired centrifuge temperature before placing in the
centrifuge and separating, otherwise allow sufficient time for them to reach the temperature in the chamber.
Display shown K2015R,K241R, K242R and
K243R (all our illustrated screens show a
chosen and actual temperature of 4oC)
Display shown when Temp is pressed
See rotor appendix for minimum
possible temperature at maximum speed
per rotor.
Displays illustrated elswhere in this
manual do not show the Temp square or the
Temperature numeric square however they
will be present on all refigerated model
displays as will set temperature and actual
temperature where appropriate (see Key to
Displays on page 1)
(Refigerated models only - K2015R,K241R, K242R and K243R)
TEMPERATURE PROGRAMMING
Pre-cool /pre-run
START button.
See instructions above detailing
use of this feature - note that this
screen will show your selected
parameters.
-10 - +40 C
-10 - +40C

Display
Display showing running screen Display showing screen when run
finished (with Buzzer selected)
PRESS Central button on right to
open lid
LOAD your samples evenly
(see Safety Instructions, page2)
FIT LID to rotor (if part of rotor type)
CLOSE Centrifuge lid pressing both
sides down firmly.
PRESS Start button (arrow triangle, bottom right)
If you require early
termination
Time, Hold, RCFt
PRESS Square bottom right.
OR in pulse mode
RELEASE when counter (seconds)
has reached desired number
PRESS run complete Tick
(only shows IF Buzzer is selected)
PRESS lid open button
(centre right)
You may now retrieve your samples
Centurion K3 range centrifuges can
store up to 108 programs, these are
convieniently grouped into 9 pages
with up to 12 names available per
page, each name can consist of up

Error code shown on display
Error code shown on display
(version number of installed firmware)
Lid Open! Error code shown on display
If Display shows Rotor out of balance
An imbalance has occurred
When centrifuge has
stopped PRESS tick
button
PRESS Lid open button
Check for:
Improperly loaded tubes
(see Safety pages)
Broken tubes or
incorrectly measured tube
If Display shows Tacho
failure
PRESS Tick button
PRESS Lid open button
Check rotor for free rotation
Are the tubes too tall?
CALL service
department
If Display shows
Lid Open!
Check lid is closed correctly
PRESS down firmly on
lid (at both sides near front)
If not then CHECK SENSOR as below
Or CALL Service Department
To check sensors go to parameters page.
First make sure Centrifuge lid is open.
Press ‘di’ (top right small button) for Diagnostics screen. (Shown below)
It reads as follows: (either 0 or 1 may show when this screen first appears, follow instructions below to perform diagnostic checks)
.... or greater (version number of installed firmware)
.....0 or 1......By rotating rotor Tacho will show 0 or 1 as rotor turns if so Tachometer is working
.....0 or 1......By rotating rotor Rotor Rec will show 0 or 1 as rotor turns if so sensor is working
.....0 or 1......By pressing rotor firmly or shaking hard Balance will show 0 or 1
.....0 or 1......By shutting the lid the Lid switch will go from 0 to 1 showing correct sensing
Centurion Scientific Ltd - All Centurion equipment is designed and manufactured under strict Quality assurance conditions
and rigorous testing and therefore should provide you with years of trouble free service. However, should you require
repairs or maintenance work on your Centrifuge please contact our service department on [phone].
For none UK customers, please contact your local Centurion distributor for service, maintenance and after sales support.

Time Set
Programmed
Rotor Set
RCFt
(Not set)
RPM
or
RCF
Set
Acceleration
Rate Set
Deceleration
Rate Set
User name Program
Last time
countdown
Open Lid
Start
Last RCFt
Time counting down
RCFt counting up
RCF actual
Programmed
Rotor Set Acceleration
Rate Set
Deceleration
Rate Set
Time Set
RCFt
(Not set)
RCF(g)
Set
Stop
User name
Actual
Temperature
(Refrigerated
only)
Temp Set
(Refrigerated
only)
Time Set
Programmed
Rotor Set
RCFt
(Not set)
RPM
or
RCF
Set
Acceleration
Rate Set
Deceleration
Rate Set
User name Program
Last time
countdown
Open Lid
Start
Last RCFt
Actual
Temperature
(Refrigerated
only)
Temp Set
(Refrigerated
only)
(See previous pages for programming instructions)

Emergency Lid release
Mechanical Emergency Door release
During a power failure, you will not be able to open the Centrifuge lid via the Display button
Mechanical override is provided to allow sample recovery
However this is for Emergency use only
This should Not be used for normal use.
Warning
Therotorcanstillbespinningatahighspeed,iftoucheditcancauseseriousinjuries.
Alwayswaittilltherotorhasstopped.
Proceedasfollows
•Make sure the Rotor has stopped (view via the port in the Lid)
•Once stopped turn off the power via the inlet Switch
•Pull the Centrifuge slightly over the bench (as shown in (a)
•You will notice one (b) or some models two plastic plug (c). Plug *
•Pop open with a screwdriver (tool) and pull downwards .
•The lid will pop open You can now retrieve your samples
AfterreplacethePlasticplugsbackintotheiroriginalposition.
Reconnectthecentrifugepowerlineoncethemainspowerhasbeenrestored.
Checkallisworkingcorrectlybyclosingthelid,wait5secondsthenpresslidopen.
If not working refit or Contact your Distributor
a
b
cc
16

Nomograph. For Rcf evaluation.
NOTE centrifuges can be programmed in Rcf(G)
Example
To find the relative centrifugal force
at a radial distance of 10 cm from
the center of rotation when
operating the centrifuge at a speed
of 3000 r.p.m., place a straightedge
on the chart connecting the 10cm
point on the Rotating Radius Scale
(A) with the 3000 r.p.m. point on
the Speed Scale (B).
Read the point at which the
straightedge intersects the Relative
Centrifugal Force Scale (C) – in this
case, 1000 x gravity.
Similarly, if the desired “r.c.f.” is
known, the necessary speed for a
given rotating radius may be
determined by connecting the two
known points and reading the
intersection of the straightedge with
the Speed Scale.
Equation for Calculating R.C.F.
R.C.F. = .00001118 x r x N2
R.C.F. = relative centrifugal force (gravities)
r = radius from center of rotation to bottom of tube (cm)
N = rotating speed (rev. per minute)
17

Care & maintenance. Centrifuge
Always keep your Centrifuge clean and free from moisture at all
times, this will help prevent corrosion.
It is recommended that your centrifuge is switched off and the lid left
open overnight to ensure that any moisture build up can evaporate.
Always ensure that lids and accessories are correctly tightened
before running the unit.
Keep a 30Cm distance around your Centrifuge.
Do not site the unit in sunlight
Do not use any kind of scourers or abrasive material to clean the
Centrifuge and ensure that a pH neutral cleaner is always used.
Use only official Centurion Scientific Ltd accessories with your centrifuge.
It is recommended that regular maintenance and cleaning of the unit and
accessories is carried out.
!!
!
!
!
!
!
!
!Yearly servicing is required to validate your warranty and increase the life
of your Centrifuge and accessories. See PrO-Care pages
10