CORNING LSE 71L User manual

Corning®LSE™
71L Shaking Incubator
INSTRUCTION MANUAL
Models
6753 (120V US)
6754 (230V EU)
(49L shown)

TABLE OF CONTENTS
1.0 GRAPHIC SYMBOLS ................................................................................................................1
2.0 SAFETY PRECAUTIONS ..........................................................................................................1
3.0 INTRODUCTION........................................................................................................................2
4.0 SPECIFICATIONS.....................................................................................................................2
5.0 UNPACKING..............................................................................................................................3
6.0 INSTALLATION .........................................................................................................................3
7.0 CONTROLS AND CALIBRATION..............................................................................................3
9.0 CARE AND MAINTENANCE...................................................................................................... 6
10.0 TROUBLESHOOTING GUIDE...................................................................................................7
11.0 TECHNICAL SUPPORT/SERVICE............................................................................................ 8
12.0 SPARE PARTS AND ACCESSORIES....................................................................................... 8
13.0 WARRANTY STATEMENT........................................................................................................9
14.0 PRODUCT DISPOSAL............................................................................................................. 10

1.0 GRAPHIC SYMBOLS
The Corning®71L shaking incubator uses internationally accepted and recognized graphic symbols
to help convey information to the user and to call the users’ attention to important safety precautions
and guides for using this equipment.
2.0 SAFETY PRECAUTIONS
Use of this product in any manner not specified by the manufacturer may impair the safety
protection provided by the equipment and may result in physical damage and/or personal injury.
Please read all operating instructions in this manual prior to use of this equipment.
1. Do not operate this unit in an explosive or flammable environment.
2. Do not incubate or shake flammable or explosive materials or highly reactive chemicals.
3. Lifting/Handling: These units are heavy and care should be taken to use appropriate lifting
devices. Units should only be lifted from their bottom surfaces and not by doors, handles, or
knobs.
4. Leveling: The unit must sit level and solidly on the four leveling feet.
5. Load testing: Test all loads to be shaken under observation to ensure load and unit
stability.
6. Units are not stackable. Do not stack one unit on top of another unit.
Indicates that user should consult manual for further description or discussion
Indicates AC “Power On”
Indicates “Degrees Centigrade”
Indicates Potential “Shock Hazard”
Indicates “Heating”
Indicates “Temperature”
Indicates Protective “Earthground”
1

3.0 INTRODUCTION
This manual covers the specifications, operation, and use of the Corning®71L shaking incubator
and its accessories. Please pay special attention to the Safety Precautions section in this manual.
The 71L shaking incubator provides an extremely stable temperature environment and an
aggressive shaking motion for the mixing of materials for bacteria and cell culture growth at ambient
and above ambient temperatures.
The 71L shaking incubator uses microprocessor controls and mechanical convection to maintain a
stable temperature environment and to achieve fast chamber temperature recovery after a door
opening. The incubator interior is constructed from stainless steel for corrosion resistance and easy
clean-up of accidental spills. All incubator doors have an integral glass window and the incubator
has a light to allow observation of samples without opening the door. Each door is fully thermal
gasketed.
The 71L shaking incubator comes with one removable full shelf and four large, rubber-padded
adjustable feet for leveling the unit. Additional half and full shelves may be purchased. The 71L
shaking mechanism supports a wide range of optional rack and flask platforms which are user
changeable.
4.0 SPECIFICATIONS
Chamber temperature range
Ambient 5°C to 80°C (Ambient +10°C with shaker
on)
Temperature accuracy
+/-0.2°C
Temperature controller
Microprocessor – user calibratable
Over temperature safety
Independently settable
Shaker speed
20 to 300 rpm
Shaker orbit
19 mm
Shaker max load
7.4 kg*
Shaker timer
Continuous (Hld) or 30 seconds to 99 minutes 50
seconds
Timer increments
30 seconds to 9 minutes 59 seconds in 1-second
steps
10 minutes to 99 minutes 50 seconds in 10-second
increments
Interior electrical outlet
1 amp, 120V unit only
Chamber volume
2.5 cu. ft./71 liters
Exterior dimensions (W x D x H)
574 x 544 x 644 mm
Chamber dimensions (W x D x H)
442 x 396 x 40 mm
Electrical Ratings and Unit Size
Model No. Description
6753 120V, 50/60Hz, 6 amps
6754 230V, 50/60Hz, 4 amps
*Maximum load is 4 x 2 liter flasks with 400 mL liquid in each flask.
2

5.0 UNPACKING
Upon receipt of your Corning®71L shaking incubator, examine the carton and unit for damage. If
shipping damage has occurred, a claim must be filed with the carrier. The carrier is responsible for
correcting shipping damages. Save all packaging until the unit has been shown to operate properly
to your satisfaction. Carefully remove the unit from the carton and shipping pallet.
The package should include:
•71L Corning Shaking Incubator Instruction Manual
•Power cord (EU and UK cords in 230V)
•1 full shelf
•2 shelf brackets
•4 adjustable feet
6.0 INSTALLATION
Install the four adjustable feet and locate the shaking incubator on a stable, flat, very solid surface
near a grounded electrical outlet. The location selected should be out of direct sunlight and away
from heat-producing sources or hot or cold air drafts. At least 5 cm ventilation clearance is required
around all sides of the incubator. Level the incubator using the four adjustable feet. Clockwise
rotation of a foot raises the incubator. Plug in the unit to a properly rated and grounded electrical
outlet and the unit will be ready for use. Install the optional platform you have selected by plugging
in the four bottom protrusions. Larger flask platforms may require being screw clamped to the
shaking mechanism.
When installing the optional shelf, it is important to note that for sufficient clearance under the shelf,
the largest flask size that can be used is the 1L flask. The shelf will also limit illumination from the
chamber and may be prone to rattle under aggressive shaking.
7.0 CONTROLS AND CALIBRATION
The controls for the 71L shaker incubator include power to the oven, temperature setting, safety
temperature overshoot setting, shaker speed, and shaking time. In addition there is a door-open
shaker shut-off switch and a chamber light switch.
Main Power Switch:This switch (illuminated GREEN when on) turns the power to the unit ON or
OFF.
3

Temperature Controller and Temperature Set:The controller has a 3-digit display for displaying
chamber temperature or set point information. UP and DOWN arrow pads are used to change the
set point and controller mode of operation. To enter the set point mode of operation, press either the
UP or DOWN arrow pad one time. The display will start to blink, going from bright to dim. While
blinking, the display is showing the set point. To change the set point, use the UP and DOWN arrow
pads. If the arrow pads are not pressed for 5 seconds, the display will stop blinking and will read the
chamber temperature. After setting temperature, allow at least one hour for the chamber
temperature to stabilize and 24 hours for optimum stabilization.
Calibration: The 71L shaking incubator is calibrated at 37°C at the factory. The unit can be re-
calibrated after the chamber temperature has stabilized at the set point for several hours. Suspend
a certified reference thermometer in the chamber. Compare the unit’s display to the reference
thermometer. If there is an unacceptable difference, put the controller into calibration mode by
pressing both the UP and DOWN arrow pads at the same time until the two outside decimal points
begin to flash. While the decimal points are flashing, the display can be calibrated to match the
reference thermometer by pressing the UP or DOWN arrow pads until the display reads the correct
value. Allow the shaker incubator to stabilize again and recalibrate if necessary.
Heating Indicator:This indicator will illuminate GREEN when the controller is calling for heat from
the heater. This indicator will be on continuously while the oven heats up to the set temperature and
will then cycle on and off at the set temperature.
Safety Thermostat:The safety thermostat located on the back of the unit is manually set and
completely independent of the Main Temperature Controller. The safety guards against any failure
of the Main Controller that would allow temperature to rise past the safety set point. If the
temperature rises to the safety set point, the Safety takes control of the heating element and allows
continued use of the incubator until the problem can be resolved or service can be arranged.
Safety Indicator: This indicator will illuminate RED when the Safety Thermostat is activated.
Under normal operating conditions this indicator should never be on.
Chamber Light Switch: This switch turns the chamber light on and off when power is on.
SHAKER CONTROLS: The shaker controls are also microprocessor-based.
•RUN lamp: This lamp illuminates when the shaker is started and stays illuminated when the
shaker is running.
•Timer lamp: This lamp illuminates when the shaker display is in the time display mode
counting down the remaining run time or showing the set run time.
•RPM lamp: This lamp illuminates when the shaker display is speed display mode showing
the current shaking speed or showing the set shaking speed.
•STOP/START: This pad starts and stops the shaking mechanism and causes the RUN
lamp to be illuminated.
4

Push to Shift/Turn Set Knob: This encoder provides the following multiple functions:
•Increases and decreases the shaker speed setting in 1 rpm increments from 20 rpm to 300
rpm.
•Increases and decreases the run time setting from 30 seconds to 99 minutes and 50
seconds and HOLD (Hld) for continuous operation. Time is changed in 1-second increments
to 10 minutes and then 10-second increments thereafter.
•It shifts the shaker display from time display to speed display and back.
Shaker Display: This three digit display shows the shaker speed setting, shaker time setting, or
remaining shaker run time while counting down. It also shows:
•Hld:When the shaker is set for continuous un-timed running
•Opn:When the incubator door has been opened, automatically stopping the shaking motion
Note: the timer continues to count down but is not displayed
•End:When a timed shaking run has ended
•F60/F50: When the shaking incubator is first powered up indicating the line frequency of the
power line
Door Open Switch: This senses when the incubator shaker door is open and sends a signal to the
microprocessor which causes the shaker to stop running as a safety measure. However, in a timed
run, the timer will continue to count down when the door is open. Closing the door restarts the
shaking motion.
8.0 OPERATING THE CORNING 71L INCUBATOR SHAKER
As advised in the installation section, it is very important that the unit be level and placed on a very
solid surface. When loading the platforms, it is also important to load these in a balanced manner or
the unit can vibrate and possibly walk. Shaking unbalanced loads for extensive periods may also
damage the shaker.
Before powering up the unit, you should first set the mechanical Safety Thermostat on the back of
the unit to a temperature just above your intended operating temperature or at maximum (setting of
10) this safety is not needed.
After you first power up the unit you should notice the shaker display first showing dashes then the
power input line frequency (F60 or F50 for 60 Hz or 50Hz) and the temperature controller display
showing some random numbers and dots blinking.
The shaker display and temperature display should then settle down with the shaker display
showing the last time that was set (or Hld) for continuous operation, and the temperature display
showing the chamber temperature.
You may now set the desired operating temperature, the desired shaking speed, and the desired
shaking time (see Controls section of this manual). It is recommended that you test all loads to be
shaken at a slow speed under observation and then gradually raise the speed to ensure the load
and unit is stable. The unit should also be allowed to equilibrate to the set temperature before using.
5

Once the temperature, shaking speed, and shaking time is set, use the STOP/START pad to initiate
shaking. When shaking in the timed mode, the word End will be displayed on the shaker display
when the shaking is done. In timed mode the word Hld (hold) will be displayed on the shaker
display. The user may switch the shaker display to show the shaking speed by pressing the Push
to Shift knob.
The user can observe the load being shaken through the glass window and can also turn on the
chamber light for additional visibility. The chamber light should not be left on for an extended
period as it adds heat to the chamber and may cause the chamber temperature to rise above
the set point.
The user may open the chamber door at any time to gain access to the chamber. If the shaker is
running when the door is open, the shaker power will be immediately cut causing the shaker to slow
and stop. The shaker display will show Opn (open). Shaking will automatically resume when the
door is closed if there is still time remaining on the shaker run. Note: The clock continues to count
down when the door is open even though the shaker has stopped.
9.0 CARE AND MAINTENANCE
No routine maintenance is required for the electrical or mechanical components of the unit. The
incubator exterior, interior, and shaking platform should be wiped down periodically with a soft damp
cloth with mild soap. Do not use chlorine-based bleach or abrasives. Any spills in the incubator
and/or on the shaking platforms should be cleaned up immediately. Be sure to disconnect the
power cord before cleaning or decontaminating the oven.
6

10.0 TROUBLESHOOTING GUIDE
Symptom
Solution
Oven will not power up or not heat
Check power cord, outlet, and unit circuit
breaker.
Shaking platform will not operate
Check timer set to Hld or a time setting.
Check that nothing is blocking shaker
platform.
Press shaker STOP/START button.
Temperature too high
Check set point and re-adjust if necessary.
Check calibration.
Chamber temperature goes above set
door point and settles back to set point
Normal operation in initial heat up or if
opened for a long period.
Temperature will not remain stable or
display shows “LO”
Check that set point is at least 5°C above
ambient (or 10°C above ambient when
shaker is on) which is minimum operating
point.
Indicated temperature is unstable
A slight variation of +/-0.1°C is normal.
Larger fluctuations may be ambient
variations from drafts, door opening and
closing, a fan obstruction or failure, or
electrical noise from RFI (motors, etc.).
Temperature is too low
If door has opened, unit may not have
recovered yet.
Confirm temperature set point.
Safety thermostat set too low.
Unit will not heat above temperature
chamber that is below set point
Confirm set point.
Check temperature with a thermometer and
recalibrate if needed.
Temperature display and reference for
thermometer do not match
Be sure that unit has been allowed to
stabilize for 1 hour.
Thermometer should be suspended in the
chamber and not touching any surface.
Only certified reference thermometers should
be used.
Cannot adjust set point, calibration
problem, or continually displays call
service
Turn unit off for 5 seconds to reset.
If persists, contact Corning for service.
Unit calibrated at one temperature load
and not at another
This can be a normal condition if
temperatures vary widely.
For best accuracy, calibrate at set point.
7

11.0 TECHNICAL SUPPORT/SERVICE
If you have a question about the Corning®LSE™71L Shaking Incubator or have a service inquiry,
contact Corning Customer Service immediately at: 1.800.492.1110 (United States and Canada),
1.978.442.2200 (outside the United Stated), or Contact your local Corning sales office.
Before returning any unit for service, a Return Authorization (RA) number must first be obtained.
Equipment sent in without our prior authorization will be returned at the customer’s expense. When
returning a unit to Corning for service, it should be sent in the original packaging. If this is not
possible, be sure that the unit is sufficiently packed. Any damage resulting from improper packaging
is the responsibility of the customer. A written explanation should accompany the unit along with the
RA number.
12.0 SPARE PARTS AND ACCESSORIES
Description
Corning
Cat. No.
Flat shaker platform with non-slip rubber mat (39 x 33 cm)
480160
Shaking platform pre-drilled for flask clamps, clamps sold separately
480159
Platform with 4 x 2L flask clamps
480161
Platform with 6 x 1L flask clamps
480162
Platform with 8 x 500 mL flask clamps
480163
Platform with 12 x 250 mL flask clamps
480164
Platform with 20 x 125 mL flask clamps
480165
Platform with 30 x 50 mL flask clamps
480166
Flask clamp, 25 mL (max. 16)
480110
Flask clamp, 50 mL (max. 16)
480111
Flask clamp, 125 mL (max. 16)
480112
Flask clamp, 250 mL (max. 9)
480113
Flask clamp, 500 mL (max. 5)
480114
Flask clamp, 1,000 mL (max. 6)
480115
Flask clamp, 2,000 mL (max. 4)
480167
Test tube rack holder (for 15 mL test tube racks), fits I-5330 platform
480168
Test tube rack holder (for 50 mL test tube racks)
480169
Shelf, full
480170
8