Dionex UltiMate 3000 Series User manual

UltiMate 3000 Series
SRD-3x00 Solvent Racks
Operating Instructions
(Original Operating Instructions)
Revision: 1.5
Date: September 2010
© 2010 Dionex
Doc. No. 4820.3550

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions

Operating Instructions Page I
Declaration of Conformity
Product: Solvent Rack
Types: SR-3000, SRD-3200,
SRD-3400, and SRD-3600
Dionex GmbH herewith declares conformity of the above products with the
respective requirements of the following regulations:
Machinery Directive 2006/42/EC
Low-Voltage Directive 2006/95/EC
EMC Directive 2004/108/EC
The safety of the machinery was evaluated based on the following standard:
EN-ISO-14121-1
Safety of machinery - risk assessment, part 1
The electrical safety of the products was evaluated based on the following
standard:
EN 61010-1: 2002
Safety requirements for electrical equipment for measurement, control and
laboratory use, Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated
based on the following standard:
DIN EN 61326: 2006
Electrical equipment for measurement, control and laboratory use
EMC Requirements
Person responsible for the technical CE documentation: Burkhard Seyferth
at Dionex Softron GmbH, Dornierstrasse 4, D-82110 Germering
This declaration is issued for the manufacturer
Dionex Softron GmbH
Dornierstraße 4
D-82110 Germering
by the President, Dr. Peter Jochum.
August 10, 2010

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page II Operating Instructions

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page i
Table of Content
1Introduction................................................................................................................... 1
1.1 How to Use this Manual ........................................................................................ 1
1.2 Safety Information .................................................................................................2
1.2.1 Symbols on the Instrument and in the Manual.................................................2
1.2.2 General Safety Precautions............................................................................... 3
1.2.3 Consignes Générales de Sécurité...................................................................... 4
1.3 Intended Use .......................................................................................................... 7
1.4 Federal Communications Commission (FCC) Note.............................................. 7
2Overview........................................................................................................................ 9
2.1 Unit Description..................................................................................................... 9
2.2 SRD Configurations............................................................................................... 9
2.3 Front Panel Elements...........................................................................................10
2.4 Rear Panel............................................................................................................ 11
2.5 Fluid Connections................................................................................................12
2.6 Leak Sensor.......................................................................................................... 13
2.7 Wellness...............................................................................................................13
3Installation................................................................................................................... 15
3.1 Facility Requirements.......................................................................................... 15
3.2 Unpacking............................................................................................................ 15
3.3 Positioning the SRD in the UltiMate 3000 System ............................................. 17
3.4 Connecting the SRD ............................................................................................ 19
3.4.1 Connecting the SRD to an UltiMate 3000 System Pump............................... 19
3.4.2 Connecting the SRD to the Main Power Supply (Optional) .......................... 19
4Preparation for Operation (Startup) ........................................................................ 21
4.1 Overview.............................................................................................................. 21
4.2 Connecting Drain Tubing .................................................................................... 21
4.3 Solvent Reservoirs ............................................................................................... 22
4.3.1 General Notes .................................................................................................22
4.3.2 Connecting the Solvent Reservoirs ................................................................23
4.3.3 Connecting the Solvent Lines from the Pump to the Degasser......................25
5Operation and Maintenance ...................................................................................... 27
5.1 Choosing the Solvents ......................................................................................... 27
5.2 General Notes for Degasser Operation................................................................ 29

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SRD-3x00 Solvent Racks
Page ii Operating Instructions
5.3 Operational Settings.............................................................................................30
5.3.1 Turning the Degasser on or off (from the UltiMate 3000 System Pump)......30
5.3.1.1 Turning the Degasser on and off from the Pump Display.......................30
5.3.1.2 Turning the Degasser on and off in Chromeleon ....................................31
5.3.2 Turning the Degasser On or Off (Stand-alone Operation) .............................32
5.4 Special Chromeleon Functions ............................................................................33
5.4.1 Leak and Vacuum Monitoring........................................................................33
5.4.2 SRD Diagnostics.............................................................................................33
5.5 Shutting Down the SRD.......................................................................................34
5.6 Routine and Preventive Maintenance ..................................................................35
6Troubleshooting ..........................................................................................................37
6.1 Overview..............................................................................................................37
6.2 Messages on the Pump Display ...........................................................................38
6.3 Chromeleon Diagnostics Message.......................................................................38
6.4 Operating Problems .............................................................................................39
7Service ..........................................................................................................................43
7.1 General Notes.......................................................................................................43
7.2 Rinsing the Degassing Channels..........................................................................44
7.3 Eliminating Leakage in the Solvent Rack............................................................45
8Technical Information................................................................................................ 47
9Accessories, Consumables, and Spare Parts ............................................................49
9.1 Standard Accessories ...........................................................................................49
9.2 Optional Accessories ...........................................................................................51
9.3 Consumables and Spare Parts ..............................................................................51
10 Pin Assignment............................................................................................................53
10.1 15-pin D-Sub Port................................................................................................53
10.2 DC Input...............................................................................................................53
11 Index.............................................................................................................................55

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 1
1 Introduction
1.1 How to Use this Manual
The layout of this manual is designed to provide quick reference to the sections of interest
to the reader. However, in order to obtain a full understanding of the Solvent Rack, Dionex
recommends that you review the manual thoroughly before beginning operation.
Almost all descriptions in the manual apply to all models in the UltiMate 3000 SRD-3x00
Solvent Rack series. Therefore, the term "the SRD" is used throughout the manual. If some
detail applies to only one model, the model is identified by name.
Note: The device configuration may vary; therefore, not all descriptions necessarily
apply to your particular instrument.
The software descriptions in this manual refer to Chromeleon 6.80 Service
Release 10.
The information contained in this manual is subject to change without notice and should
not be construed as a commitment by Dionex. Dionex assumes no responsibility for any
errors that may appear in this document. This document is believed to be complete and
accurate at the time of publication. In no event shall Dionex be liable for incidental or
consequential damages in connection with or arising from the use of this document.
The information contained in this document is subject to change without notice.
CHROMELEON®and UltiMate®are registered trademarks of Dionex Corporation in the
United States of America and/or other countries. Viper™ and nanoViper™ are trademarks of
Dionex Corporation in the United States of America and/or other countries. All other trade or
company names mentioned are subject to the copyright and the property and trademark rights
of the respective companies.
All rights reserved, including those for photomechanical reproduction and storage on
electronic media. No part of this publication may be copied or distributed, transmitted,
transcribed, stored in a retrieval system, or transmitted into any human or computer language,
in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or
disclosed to third parties without the express written permission of Dionex.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 2Operating Instructions
1.2 Safety Information
The CE Mark label and cTUVus Mark safety label on the instrument indicate that the
instrument is compliant with the related standards.
1.2.1 Symbols on the Instrument and in the Manual
The table shows the symbols used on the instrument:
Symbol Description
˜
Alternating current—Courant alternatif
Refer to the Operating Instructions to prevent risk of harm to the operator and to
protect the instrument against damage.
Référez-vous à ce manuel pour éviter tout risque de blessure à l'opérateur et/ou
protéger l'instrument contre tout dommage
Label according to the "Measures for Administration of the Pollution Control of
Electronic Information Products" (China RoHS) guideline
Étiquette "Measures for Administration of the Pollution Control of Electronic
Information Products" (China RoHS)
WEEE (Waste Electrical and Electronic Equipment) label—For more
information, see the WEEE Information section in the "Installation and
Qualification Documents for HPLC Systems" binder. Étiquette WEEE (Waste
Electrical and Electronic Equipment) —Pour plus d'informations, référez-vous
au chapitre WEEE Information dans le classeur "Installation and Qualification
Documents for HPLC Systems"
At various points throughout the manual, messages of particular importance are indicated
by certain symbols:
Tip: Indicates general information, as well as information intended to
optimize the performance of the instrument.
Important: Indicates that failure to take note of the accompanying information
could cause wrong results or may result in damage to the instrument.
Important: Indique que ne pas tenir compte de l'information jointe peut conduire
à de faux résultat ou endommager l'instrument.
Warning: Indicates that failure to take note of the accompanying information
may result in personal injury.
Avertissement: Indique que ne pas tenir compte de l'information jointe peut
entraîner des blessures corporelles.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 3
1.2.2 General Safety Precautions
When working with analytical instrumentation, you should know the potential hazards of
using chemical solvents.
Tips: Before initial operation of the SRD, make sure that you are familiar with the
contents of this manual.
Observe any warning labels on the device and see the related sections in these
Operating Instructions.
To avoid the possibility of personal injury or damage to the instrument, observe the
following general safety precautions when operating the instrument or carrying out any
maintenance work:
Install the HPLC system in a well-ventilated laboratory. If the mobile phase includes
volatile or flammable solvents, do not allow them to enter the workspace.
For minimum interference effects, all components of the analytical system should be
connected to the same mains output (same phase).
The front panel tilts upward. When lifting or moving the SRD, always lift by the bottom
or sides of the instrument. This is to avoid damage to the instrument.
For safety reasons, you should not lift the SRD with one or more solvent reservoirs being
in the tray.
The open front panel door is not designed to carry weight. Therefore, you should not
place any objects on the open front panel door.
Always set a lower pressure limit for the HPLC pump. This prevents damage resulting
from leakage or from running the pump dry over a longer period. Activate liquid level
monitoring for the solvent reservoirs (Pump Manual).
If the mobile phase includes volatile or flammable solvents, avoid open flames and
sparks.
If a leak occurs, turn off the SRD and remedy the situation immediately.
Replace faulty power cords and communication cables.
Many organic solvents and buffers are toxic. Know the toxicological properties of all
mobile phases that you are using.
The toxicological properties of many samples may not be well known. If you have any
doubt about a sample, treat it as if it contains a potentially harmful substance.
Wear goggles when handling mobile phases or operating the instrument. An eyewash
facility and a sink should be close to the unit. If any mobile phase splashes on the eyes or
skin, wash the affected area and seek medical attention.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 4Operating Instructions
Dispose of waste mobile phase in an environmentally safe manner that is consistent with
all local regulations. Do not allow flammable or toxic solvents to accumulate. Follow a
regulated, approved waste disposal program. Never dispose of flammable or toxic
solvents through the municipal sewage system.
Use only standard solvents (HPLC grade) and buffers that are compatible with all parts
that may be exposed to solvents (page 47).
In an UltiMate 3000 system, some components are made of PEEK™. While this polymer
has superb chemical resistance to most organic solvents, it tends to swell when in contact
with trichlormethane (CHCl3), dimethyl sulfoxide (DMSO), or tetrahydrofuran (THF). In
addition, it is attacked by concentrated acids, such as, sulfuric acid and nitric acid or a
mixture of hexane, ethyl acetate, and methanol. (Swelling or attack by concentrated acids
is not a problem with brief flushing procedures.)
Do not use PEEK tubing that is stressed, bent, or kinked.
Wear goggles when handling fused silica tubing (during installation, cutting, etc.)
Before interrupting operation for several days or more, observe the precautions on
page 34.
Use original Dionex spare parts only. Substituting non-Dionex parts or using non-Dionex
accessories may impair the performance of the SRD.
Do not use the SRD in ways other than those described in these operating instructions.
1.2.3 Consignes Générales de Sécurité
Veuillez noter: Avant de commencer à utiliser l'instrument, assurez-vous que vous
vous êtes familiarisés avec le contenu de ce manuel.
Observez les étiquettes d'avertissement sur l'appareil et référez-vous
aux sections correspondantes dans ce mode d'emploi.
Veuillez observer les consignes générales de sécurité suivantes lorsque vous utilisez
l'instrument ou que vous procédez à des opérations de maintenance:
Installez le système HPLC dans un laboratoire bien ventilé. Si la phase mobile contient
des solvants volatils ou inflammables, empêchez qu'ils ne pénètrent dans l'espace de
travail.
Afin d'éviter au maximum les interférences, tous les éléments du système analytique
doivent être raccordés à la même ligne secteur (même phase).
Lorsque vous soulevez ou l’instrument, tenez-le toujours par le dessous ou par les côtés
de l'unité. Soulever l’instrument par la partie avant inférieure ou par le panneau avant peut
endommager la porte.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 5
Pour raison de sécurité, ne soulevez pas l'instrument avec des réservoirs.
Ne placez aucun objet lourd sur la porte ouverte du panneau avant. Ceci pourrait
endommager la porte.
Réglez toujours une limite de pression minimum pour le système HPLC. Ceci prévient les
dommages résultant de fuites ou de long-terme fonctionnement à sec de la pompe.
Activez la surveillance de niveau liquide pour des réservoirs (manuel de pompe).
Si la phase mobile contient des solvants volatils ou inflammables, évitez les flammes nues
et les sources d’étincelles à proximité.
Si une fuite se produit, arrêtez immédiatement l’instrument et remédiez au problème.
Remplacez les cordons d'alimentation électrique et les câbles de communication
défectueux.
De nombreux solvants organiques et solutions salines sont toxiques. Informez-vous des
propriétés toxicologiques de toutes les phases mobiles que vous utilisez.
Les propriétés toxicologiques de nombreux échantillons peuvent être mal connues. Au
moindre doute concernant un échantillon, traitez-le comme s'il contenait une substance
potentiellement dangereuse.
Portez des lunettes de protection lorsque vous manipulez des phases mobiles ou que vous
utilisez l'instrument. Une installation permettant de se laver les yeux ainsi qu'un lavabo
doivent se trouver à proximité du système. Si une phase mobile, quelle qu'elle soit, entre
en contact avec vos yeux ou votre peau, rincez abondamment la zone affectée à l’eau,
puis.
Débarrassez-vous de tous les déchets de phase mobile de manière écologique,
conformément à la règlementation en vigueur au niveau local. Empêchez impérativement
l'accumulation de solvants inflammables et/ou toxiques. Suivez un programme
d'élimination des déchets règlementé et approuvé. Ne jetez jamais de solvants
inflammables et/ou toxiques dans le système municipal d'évacuation des eaux usées.
Utilisez uniquement des solvants (qualité HPLC) et des solutions salines compatibles
avec les matériaux exposés phase mobiles (page 47).
Dans un système UltiMate 3000, certaines composantes sont en PEEK. Bien que ce
polymère présente une excellente résistance chimique à la plupart des solvants
organiques, il a tendance à gonfler lorsqu'il est en contact prolongé avec du chloroforme
(CHCl3), du diméthyle sulfoxyde (DMSO) ou du tétrahydrofurane (THF). De plus, il est
attaqué par des acides concentrés tels que l'acide sulfurique et l'acide nitrique ou d'un
composé du hexane, éthyle acétate et méthanol. (Ces acides peuvent cependant être
utilisés dans le cadre de procédures de nettoyage, à condition que l’exposition soit brève.)

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 6Operating Instructions
N'utilisez pas de tubes PEEK écrasés, pliés ou abimés.
Portez des lunettes de protection lorsque vous manipulez des capillaires en silice fondue
(pendant l'installation, découpe, etc.).
Avant d'interrompre le fonctionnement pendant plusieurs jours ou plus, observez les
précautions figurant sur la page 34.
Utilisez des pièces de rechange d'origine Dionex. Effectuer des remplacements par des
pièces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex
peut affecter les performances de l'instrument.
N'utilisez pas l'instrument de manière autre que celles décrites dans ce manuel.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 7
1.3 Intended Use
The SRD has been developed as part of the UltiMate 3000 system and is a reliable module
for the secure and functional positioning of the solvent reservoirs and for automatic
degassing of typical HPLC (high performance liquid chromatography) solvents. The SRD
is controlled by the Chromeleon Chromatography Management System from the HPLC
pump of the UltiMate 3000 series. However, the SRD can be used with other HPLC
systems if adequate control inputs and outputs are available.
Being part of the UltiMate 3000 system, the SRD (and the pump) can also be operated with
other data systems, such as Analyst®(AB Sciex), Compass™/ HyStar™ (Bruker Daltonics),
or Xcalibur™ (Thermo Scientific). To do so, installation of the DCMSLink (Dionex
Chromatography Mass Spectrometry Link) software is required in addition to the installation
of the data system.
Operation in stand-alone mode is possible as well.
Please note that the SRD may be operated only by using Dionex accessories and spare parts
(page 49) and within its technical specifications (page 47).
If there is any question regarding appropriate usage, contact Dionex before proceeding.
Dionex cannot be held liable for any damage, material or otherwise, resulting from
inappropriate or improper use of the instrument.
1.4 Federal Communications Commission (FCC) Note
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference, in which case the user will be required to
correct the interference at his expense.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 8Operating Instructions

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 9
2 Overview
2.1 Unit Description
The SRD is a modern high-quality instrument designed for HPLC analysis, especially as
part of the UltiMate 3000 system. The SRD provides a secure location for installation of
the solvent reservoirs and, when mounted on top of the UltiMate 3000 system, saves
valuable bench space.
The SRD includes a reservoir tray, solvent reservoirs, and appropriate tubing. The
integrated analytical vacuum degasser continuously degasses solvents through special
polymer membranes that are permeable to gas, but not to liquids. Degassing solvents
guarantees reproducibility and reduces pulsation in the connected pump.
All parts in the flow path are made of Teflon®AF, PEEK, FEP, and Tefzel®(filter frit in the
inline filter: stainless steel or titanium) to provide optimum resistance to the most commonly
used HPLC solvents and buffer solutions.
2.2 SRD Configurations
The SRD is available in the following configurations:
SRD Description Part No.
SRD-3600 SRD with analytical 6-channel vacuum degasser
typically for use with the following pumps:
- one DGP-3600
- two HPG-3200 pumps in a two-stack system
- one HPG-3200 and one HPG-3400 in a two-stack system
- one HPG-3400 if you want to degas the solvents and the wash
liquid of the UltiMate 3000 autosampler.
5035.9230
SRD-3400 SRD with analytical 4-channel vacuum degasser
typically for use with the following pumps:
- one HPG-3400
- two HPG-3200 pumps in a two-stack system
- one HPG-3200 or ISO-3100 if you want to degas the solvents and
the wash liquid of an UltiMate 3000 series autosampler
- one NCS-3500RS
5035.9245
SRD-3200 SRD with analytical 2-channel vacuum degasser
typically for use with the following pumps:
- one HPG-3200
- one ISO-3100
5035.9250
In addition, a solvent rack without degasser channels is available (SR-3000, part no.
5035.9200). The SR-3000 is intended only for use as a solvent organizer, for example,
together with an LPG-3400 pump. For this pump type, the degasser is incorporated in the
pump.
Tip: An SRD with an analytical degasser cannot be used with a semipreparative
pump.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 10 Operating Instructions
2.3 Front Panel Elements
2
1
Fig. 1: Front panel view
No. Front Panel
Element
Description
1 LEDs
Power The LED is blue when the SRD power is on.
Vacuum The LED is green if the degasser is working properly.
The LED is red if the degasser vacuum is insufficient for proper degassing.
In this case, also the Status LED is red
Status The LED is green if the degasser is working properly and no leaks have
been detected.
The LED is red if the leak sensor reports a leak.
The Vacuum and Status LEDs are red if the degasser vacuum is
insufficient for proper degassing.
2 Standby button Switches the SRD to standby mode. The LED of the standby button is red
when the SRD is in standby mode.
To resume operation, press the standby button again (the LED is not
lighted).
Note: To allow the SRD to change the mode, press and hold the Standby
button for at least 1 second.
Important: If you switch a pump to which an SRD-3x00 Solvent Rack is
connected to the Standby mode, the Solvent Rack, too, will be set to
Standby mode.
Important: Si vous commutez une pompe à laquelle est raccordé un dégazeur
SRD, en mode Veille, le dégazeur passera également en mode
Veille.

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 11
2.4 Rear Panel
Fig. 2: Rear panel view
No. Description
1 15-pin D-Sub port: Connects the SRD to the pump (page 19)
2 DC Input (Power)
Only to be used if the SRD is not controlled by the UltiMate 3000 system pump.
In this case, connect an external power supply unit (part no. 6510.0004) from
here to the main power supply (page 19).
2 1

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 12 Operating Instructions
2.5 Fluid Connections
The front panel door tilts upward to provide easy access to the fluid connections in the
SRD. The open door locks in its topmost position.
Fig. 3: SRD with open front panel door (here SRD-3600)
No. Description
1 Exhaust air outlet
Optional: Connect tubing to vent vapors exiting the degassing chambers.
2 Tubing guides for the solvent lines
3 Leak sensor (page 13)
Warning: For safety reasons, you should not lift the SRD with one or more
solvent reservoirs being in the tray.
Avertissement: Pour raison de sécurité, ne soulevez pas l'instrument avec des
réservoirs.
Important: Do not lift the SRD by the front panel door. When lifting or moving
the SRD, always lift by the bottom or sides of the instrument. Lifting
the SRD by the front panel may damage the front panel door.
The open front panel door is not designed to carry weight. Therefore,
you should not place any objects on the open front panel door.
Important: Lorsque vous soulevez ou l’instrument, tenez-le toujours par le
dessous ou par les côtés de l'unité. Soulever l’instrument par la partie
avant inférieure ou par le panneau avant peut endommager la porte.
Ne lacez aucun objet sur la porte ouverte du panneau avant. Ceci
peut endommager la porte.
12
3

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Operating Instructions Page 13
2.6 Leak Sensor
A leak sensor is installed inside the SRD. If liquid collects in the drip tray under the fluid
connections, the leak sensor reports a leak, and the Status LED on the front panel door
changes to red.
If the SRD is operated from a pump of the UltiMate 3000 system and if the leak sensor is
exposed to liquid for at least 30 seconds, a message (Solvent Rack leak detected) appears on
the front panel display of the pump and a beep sounds. If the pump is operated with
Chromeleon, a message is also displayed in the Chromeleon Audit Trail.
When the leak sensor reports a leak, eliminate the cause for the leakage and dry the leak
sensor (page 45).
2.7 Wellness
System Wellness monitors the health of the SRD. Therefore, the SRD supports several
performance and reliability features that can help you detect small problems before they
turn into big ones:
Leak detection–Status LED on the front panel door
Degasser vacuum level monitoring–Status and Vacuum LEDs on the front panel door
When an error is detected, the Status LED or the Status and Vacuum LEDs on the front
panel door change to red. If the SRD is connected to an UltiMate 3000 system pump that is
operated with Chromeleon, the associated message appears on the pump display and also in
the Chromeleon Audit Trail (page 37).

UltiMate 3000 Series:
SRD-3x00 Solvent Racks
Page 14 Operating Instructions