Drawell DU-8200 Series User manual

USER MANUAL
DU-8200 Series
UV Visible Spectrophotometers
Drawell International Technology Limited
Chongqing Drawell Instrument CO,.Ltd
Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China
Tel: [phone]
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561XiuChuan Rd.,PuDong New Area,Shanghai,China
Web : www.drawell.com.cn
Email : [email protected]

Contents
Chapter 1 Preface……………………………………………………………………………1
1. Safety………………………………………………………………………………1
2. General……………………………………………………………………………………1
3. Electrical …………………………………………………………………………………1
4. Warning…………………………………………………………………………………………………….. 1
5. Performance……………………………………………………………………………………………….. 2
6. Radio Interference ………………………………………………………………………………………2
Chapter 2 Introduction of the Instrument.........................................................3
1. Brief Introduction of the instrument...................................................................3
2. Working Principle………………………………………………………………………4
3. Structure...........................................................................................4
4. Unpacking Instructions………………………………………………………………………………….5
5. Specifications……………………………………………………………………………………………….5
Chapter 3 Install Instrument……………………………………………………………6
1. Environment Required…………………………………………………………………6
2. Voltage Confirmation…………………………………………………………………6
3. Installation………………………………………………………………………………6
Chapter 4 Operation Introduction………………………………………………………7
1. Software Structure………………………………………………………………………7
2. Basic Operation…………………………………………………………………………8
2.1 Choose Photometric Mode……………………………………………………… 8
2.2 Set Wavelength …………………………………………………………………… 8
2.3 Set Parameters…………………………………………………………………… 8
2.4 Set the position of auto-cell holder……………………………………………… 8
2.5 Delete the Input Value…………………………………………………………… 8
2.6 Delete the test result and stored data……………………………………………8
2.7 Calibrate 100%T/0Abs…………………………………………………………… 8
2.8 Measure Samples………………………………………………………………… 8
2.9 Print the test result…………………………………………………………………9
3. Preparation before test………………………………………………………………9
3.1 Switch on the instrument and begin the system self-test………………………9
3.2 Pre-warm……………………………………………………………………………9
3.3 Check the cuvettes…………………………………………………………………9
4. Measurement……………………………………………………………………………9
4.1 Photometry…………………………………………………………………………9
4.2 Quantitative………………………………………………………………………11
4.3 Setup ……………………………………………………………………………17

1) Power On/Off D2 Lamp………………………………………………………18
2) Power On/Off W Lamp………………………………………………………18
3) Set Date and time……………………………………………………………19
4) Calibrate Dark Current………………………………………………………19
5) Wavelength Calibration………………………………………………………20
6) Input the lamps changing Wavelength Point………………………………20
7) Reload Default…………………………………………………………………21
8) Version…………………………………………………………………………21
Chapter 5 Instrument Maintenance……………………………………………………22
1. Daily Maintenance……………………………………………………………………………………….22
2. Trouble shooting………………………………………………………………………………………….22
3. Sparepart Replacement………………………………………………………………………………..23

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CHAPTER 1 PREFACE
1, Safety
The safety statements in this manual comply with the requirements of the HEALTH
AND SAFETYAT WORKACT, 1974.
Read the following before installing and using the instrument and its accessories.
This instrument should be operated by appropriate laboratory technicians.
2, General
The apparatus described in this manual is designed to be used by properly trained
personnel in a suitable equipped laboratory. For the correct and safe use of this
apparatus it is essential that laboratory personnel follow generally accepted safe
procedures in addition to the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of
the power supply unit is a hazardous area and its cover should not be removed under
any circumstances. There are no serviceable components inside this power supply
unit. For this instrument, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inflammable
and samples may be radioactive, toxic, or potentially infective. Care should be taken
to follow the normal laboratory procedures for handling chemicals and samples.
3, Electrical
Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth contact.
The protective action must not be negated by the use of an extension cord without a
protective conductor.
4, Warning
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be
made inoperative and be secured against any unintended operation.
Note：NEVER touch or handle the power supply on this instrument due to the high
voltage！
The protection is likely to be impaired if, for example, the apparatus

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▲Shows visible damage
▲Fails to perform the intended measurements
▲Has been subjected to prolonged storage under unfavorable conditions
▲Has been subjected to severe transport stresses
5, Performance
To ensure that the instrument is working within its specification, especially when
making measurements of an important nature，carry out performance checks with
particular reference to wavelength and absorbance accuracy. Performance checks are
detailed in this manual.
6, Radio Interference
For compliance with the EMC standards referred to in the EC Declaration of
Conformity, it is necessary that only shielded cables supplied by us are used when
connecting the instrument to computers and accessories.

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CHAPTER 2 INTRODUCTION OF THE INSTRUMENT
1. Brief Introduction of the Instrument
This instrument is a single beam, general purpose instrument designed to meet the
needs of the Conventional Laboratory, This instrument is ideal for various applications,
such as: Chemistry, Biochemistry, Petro-chemistry, Environmental Protection, Food
and Beverage Labs, Water and Waste Water Labs and other fields of quality control
and research.
This instrument incorporates a 128×64 dots matrix LCD display for photometric
results, easy operation and wavelength range of 190nm to 1100nm. This instrument is
ideal for measurements in the visible and ultraviolet wavelength region of the
electromagnetic spectrum.
Fig 2-1 Front View
Fig 2-2 Control Panel

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2. Working Principle
Different matter has different but special absorbance wavelength point. Also, when at
the fixed wavelength point, the absorbance has some relation to the substance’s
(Always transparent Solution) concentration and its thickness. The relation can be
concluded as the following Formula which is called Lambert-Beer Law.
T= I/IoA=KCL= -log I/Io
A Absorbance
C Concentration of the Solution
K Absorbance Coefficient of the Solution
L The length of the Solution in the light path
I The intensity of the light focused on the A/D after it permeate the
solution to be measured.
IoThe intensity of the light focused on the A/D after it permeate the
Solution.
Note: When test, the solvent is usually taken as the Reference Solution and its
Transmittance is considered as 100%T. While the Transmittance of the sample to
be tested is a relative value which is got comparing to that of the Reference.
3. Structure
The spectrophotometer consists of five parts:
1) Halogen or deuterium lamps to supply the light;
2) A Monochromator to isolate the wavelength of interest and eliminate the unwanted
second order radiation;
3) A sample compartment to accommodate the sample solution;
4) Detector to receive the transmitted light and convert it to an electrical signal; and
5) A digital display to indicate absorbance or transmittance. The block diagram (Fig
2-4) below illustrates the relationship between these parts.
Fig 2-4 Block diagram for the Spectrophotometer
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The
grating disperses the light beam to produce the spectrum, a portion of which is focused
on the exit slit of the monochromator by a collimating mirror. From here the beam is
passed to a sample compartment through one of the filters, which helps to eliminate
unwanted second order radiation from the diffraction grating. Upon leaving the
sample compartment, the beam is passed to the silicon photodiode detector and
causes the detector to produce an electrical signal that is displayed on the digital
display.

- 5 -
4. Unpacking Instructions
Carefully unpack the contents and check the materials against the following packing
list to ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer...............................................................…….…1
Power Cord...........................................................................…….…1
1cm Glass Cuvettes………………………………………..…………… 1 Set of 4
1cm Quartz Cuvettes………………………………………...…………. 1 Set of 2
Operation Manual.................................................................…….…. 1
5. Specifications
UV-VIS
VIS
Wavelength Range
190-1100nm
320-1100nm
Band Width
2nm
4nm
Wavelength Accuracy
±1nm
Wavelength Repeatability
0.3nm
Wavelength Setting
Auto
Photometric Accuracy
±0.5%T
Photometric Repeatability
0.3%T
Photometric Display Range
0-200%T, -0.3-3.0A
Stability
0.002A/h @ 500nm
Stray Light
≤0.2%T@220nm, 360nm
Data Output Port
USB
Display
128*64 Dots LCD
Lamps
Deuterium Lamp & Tungsten Halogen Lamp
Detector
Silicon Photodiode
Power Requirement
220V/50Hz or 110V/60Hz
Dimension (L*W*H)
431*318*195mm
Net Weight
12kg

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CHAPTER 3 INSTALL INSTRUMENT
1. Environment Required
To ensure the best performance, the following conditions are required:
The best working temperature range is 16-30℃and the humidity is 45-80%.
Keep it as far as possible away from the strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields.
Set the unit up in an area that is free of dust, corrosive gases and strong
vibrations.
Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
The power requirement is 220± 22V@50± 1Hz or 110± 11V@60± 1 Hz。
Use the appropriate power cord and plug into a grounded outlet.
If the local voltage is not stable enough, a manostat is required.
Be away from direct sunlight
2. Voltage Confirmation
Be sure to set the instrument’s Voltage Switch at your local power
supply, or severe damage may occur! The Voltage Switch is on the
bottom of the instrument. （The default value is 220V, you can see
the value on the Voltage Switch）
3. Installation
Step 1: Check the packing list
Unpack the contents, check the materials with the Packing List. Any damage or
Lost found, please contact us or the local dealer.
Step 2: Position
Place the instrument on the stable table carefully.
Step 3: Install printer (Optional)
Make sure the printer is power off; link the printer’s data cable to the
instrument’s parallel port.
Step 4: Link the power cord
Make sure the instrument’s power switch is in the Off condition, link the power
cord to the instrument and insert another end in the socket provided with a
protective earth contact.
Step 5: Switch on the power
Check again, make sure that all the links are right. Switch on the power. The
instrument can be used to measure after self-test and pre-warm.

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CHAPTER 4 OPERATION INTRODUCTION
1. Software Structure
1.1 Menu Tree
Main
Menu Basic Mode
Quantitative
Setup
Absorbance
Transmittance
Energy
Set
Measurement Measure
Print
Standard Curve
Set Wavelength
Coefficient Choose Equation
Coefficient K
Coefficient B
Conc. Unit
Set WL.
Measurement Measure
Print
Measurement Measure
Print
Conc. Unit
Qty. of Standards
Conc. Of Standards
Display the Curve
Select Curve
Set Parameters
Set Parameters
D2 Lamp
W Lamp
Time Set
Get Dark Current
WL. Calibration
Version
Load Default
Fig 4-1 Menu Tree
1.2 Firmware Functions
The firmware consists of 3 functions: Basic Mode, Quantitative, System Functions.
1) Basic Mode
Measure the Absorbance, Transmittance or Energy in a fixed wavelength point.
The test result can be stored in the RAM.
2) Quantitative
Standard Curve Method：Set up standard curve by standard samples; use the
new curve to measure the unknown sample’s concentration. The curves and the
test results con be stored in the RAM.

- 8 -
Coefficient Method: Input the values of the curve equation’s coefficient,
and then measure the unknown sample’s concentration.
3) Setup
Light source management; Get Dark Current; Calibrate Wavelength and Set time,
etc.
2. Basic Operation
2.1 Choose Photometric Mode
Press Arrow key to choose and
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to confirm.
2.2 Set Wavelength
In the measurement Interface，press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to set wavelength，use Arrow
keyboard to input the value，press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to confirm and set 100%T/0Abs
automatically.
2.3 Set Parameters
Press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to go into the parameter setting interface，press Arrow Key to
choose the corresponding menu and use Arrow key to input the parameter, press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to confirm，press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to return.
2.4 Set the position of auto-cell holder. （The Auto Cell Holder is optional）
In the measurement Interface, press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
first, then press the Arrow key of
1-8, and then the corresponding cell will be positioned at the light path.
2.5 Delete the Input Value
Press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to delete the last character of the input.
2.6 Delete the test result and stored data
In the measurement Interface，press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to delete the test result and
stored data.
2.7 Calibrate 100%T/0Abs
Put the reference in the light path, press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to calibrate 100%T/0Abs。
2.8 Measure the samples

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Put the samples in the light path and press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to measure.
2.9 Print the test result
In the Measurement Interface, press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to print the result..
3. Preparation before test
3.1 Switch on the instrument and begin the system self-test(Diagnostic
Program)
Make sure to remove all the blocks in the light path; Close the compartment lid
and switch on the power; Then the system will begin self-test.
Note: During the Diagnostic Program runs, please DON’T open the lid of
the compartment!
3.2 Pre-warm
When the diagnostic program finished, it goes into pre-warm condition. 20
minutes is required before measure.
3.3 Check the Cuvettes
The cuvette should be clear and there’s no solution remains on the transparent
surface. If the wavelength is below 340nm, Silicon Cuvette is a must.
4. Measure
4.1 Basic Mode
Step 1, Go into Basic Mode interface
In the main menu, use Arrow Key to choose “Baisc Mode”，press
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
to go into corresponding setting interface.（Fig. 4-2）；
Basic Mode
Quantitative
Setup 08:08
08/26
Basic Mode
0.000 Abs
500.0 nm
ESC
ENTER
Fig. 4-2
Step 2, Set Wavelength

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Press
Basic Mode
0.000 Abs
500.0 nm
Basic Mode
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
to set Wavelength，input the wavelength value by the Arrow
Keypad, then press
Basic Mode
0.000 Abs
500.0 nm
Basic Mode
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
to confirm.（Fig. 4-3）；
Basic Mode
0.000 Abs
500.0 nm
Basic Mode
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
Fig. 4-3
Step 3, Set Photometric Mode
Press
Basic Mode
0.000 Abs
500.0 nm
√Basic Mode
Abs √
%T
Energy
ESC
SET
to go into Parameter Setting Interface，press Array Key to
select “Abs”、“%T”or “Energy”mode，press
Basic Mode
0.000 Abs
500.0 nm
√Basic Mode
Abs √
%T
Energy
ESC
SET
to confirm. Press
Basic Mode
0.000 Abs
500.0 nm
√Basic Mode
Abs √
%T
Energy
ESC
SET
to return（Fig. 4-4）；
Basic Mode
0.000 Abs
500.0 nm
√Basic Mode
Abs √
%T
Energy
ESC
SET
Fig. 4-4
Step 4, Go into Continuous Measurement Interface
Press
Basic Mode
0.000 Abs
500.0 nm ESC
START
ENTER 500.0nm 0.000Abs
No. WL. Abs.
to go into continuous measurement interface（Fig. 4-5）；
Basic Mode
0.000 Abs
500.0 nm ESC
START
ENTER 500.0nm 0.000Abs
No. WL. Abs.
Fig. 4-5

- 11 -
Step 5, Calibrate 100%T/0Abs
Put the Reference Solution in the light path，press
ZERO
to calibrate
100%T/0Abs；
Step 6, Measure Sample
Put the unknown concentration sample in the light path, press
START
STOP
to
measure，and the result displays in the data sheet. At the same time, the test
result will be stored in the RAM automatically.
Repeat this step to finish all the samples measurement.
Step 7, Print the data
Press
PRINT
to print the test result.
Step 8, Delete the data
Press
ENTER
to delete the test data.
4.2 Quantitative
Step 1: Go into Quantitative Interface
In the main menu, press Arrow Key to choose “Quantitative”; Press
ENTER
to enter the Quantitative method selecting Interface.（Fig. 4-6）；
Basic Mode
Quantitative
Setup 12:00
05/26
√Quantitative
Standard Curve
Coefficient
Unit
ESC
ENTER
Fig. 4-6
Step 2: Choose Unit
Move the cursor on “Unit”, press
ENTER
to go into concentration unit
choosing interface. (Fig. 4-7) Use Arrow Key to move the cursor on the unit
you want, and press
ENTER
to confirm.

- 12 -
ESC
ENTER
√Quantitative
Standard Curve
Coefficient
Unit
1. %
2. ug/l
3. mg/l
4. g/l
5. ml/l
6. mg/ml
7. ug/ml
8. None
Fig. 4-7
Step 3: Choose Methods
There are two methods to be selected: Standard Curve and Coefficient.
I. Standard Curve：
(1) Go into “Standard Curve”Interface
In “Quantitative”menu, press Arrow Key to choose “Standard Curve”;
Press
ENTER
to go into sub menu.（Fig. 4-8）； Here you can create
a new curve, or you can load a saved curve. If you want to delete a
saved curve, just choose “Delete Curve”.
StandardCurve
ESC
ENTER
√Quantitative
StandardCurve
Coefficient
Unit
CreateCurve
LoadCurve
DeleteCurve
Fig. 4-8
(2) Set Wavelength
★Move the cursor on “Create Curve”, the press
ENTER
to go into
the sample quantity setting interface. (Fig. 4-9)
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
500.0nm 0.000Abs
Input Stds. No.
No.= 3
Fig. 4-9
★Press
Quantitative
WL=500.0nm
Please Input WL.
WL=
GOTOλ
500.0nm 0.000Abs
Input Stds No:
No.= 3
to go into the wavelength setting interface，input the
WL. Value by the Arrow Keypad and press
Quantitative
WL=500.0nm
Please Input WL.
WL=
GOTOλ
500.0nm 0.000Abs
Input Stds No:
No.= 3
to confirm.（Fig.
4-10）；

- 13 -
Quantitative
WL=500.0nm
Please Input WL.
WL=
GOTOλ
500.0nm 0.000Abs
Input Stds No:
No.= 3
Fig. 4-10
★Use the Arrow Key to input the quantity of the standard samples,
then press
ENTER
500.0nm 0.000Abs
Quantitative:
No.= 6
500.0nm 0.000Abs
Put 1# Standard,
Input 1# Conc.:
C=
to confirm. The system will go into the
following interface. (Fig. 4-11)
ENTER
500.0nm 0.000Abs
Quantitative:
No.= 6
500.0nm 0.000Abs
Put 1# Standard,
Input 1# Conc.:
C=
Fig. 4-11
(3) Calibrate 100%T/0Abs
Put the Reference Solution in the light path，then press
ENTER
500.0nm 0.100Abs
Put 2# Standard,
Input 2# Conc.:
C=
500.0nm 0.100Abs
Put 1# Standard,
Input 1# Conc.:
C= 0.020
to
calibrate 100%T/0Abs；
(4) Input Standard Samples’Concentration
★Pull the 1# Standard Sample in the light path and input the value of
its concentration by the Arrow keypad, then press
ENTER
500.0nm 0.100Abs
Put 2# Standard,
Input 2# Conc.:
C=
500.0nm 0.100Abs
Put 1# Standard,
Input 1# Conc.:
C= 0.020
to
confirm. The system will go into the next interface.
ENTER
500.0nm 0.100Abs
Put 2# Standard,
Input 2# Conc.:
C=
500.0nm 0.100Abs
Put 1# Standard,
Input 1# Conc.:
C= 0.020
Fig. 4-12
★Pull the 2# Standard Sample in the light path and input its
concentration, then press
ENTER
0.000 0.600
0.600
0.000
r=1.000
C=1.000XA+0.000
500.0nm 0.600Abs
Put 6# Standard,
Input 6# Conc.:
C=
to confirm.
★Repeat the above step till the last sample’s concentration is
finished, press
ENTER
0.000 0.600
0.600
0.000
r=1.000
C=1.000XA+0.000
500.0nm 0.600Abs
Put 6# Standard,
Input 6# Conc.:
C=
to confirm. Then the Standard Curve and its
equation will display on the screen automatically. (Fig. 4-13) At the

- 14 -
same time, the Curve Equation will be saved in the RAM
automatically.
ENTER
0.000 0.600
0.600
0.000
r=1.000
C=1.000XA+0.000
500.0nm 0.600Abs
Put 6# Standard,
Input 6# Conc.:
C=
Fig. 4-13
Note: If there’s some mistake during the operation, the system will beep
3 times and return the initial interface automatically. Of course, the
curve can not be displayed on the screen.
(5) Measure Samples
★Pull the Reference solution in the light path and press
ENTER
ESC
0.000 0.600
0.600
0.000
r=1.000
C=1.000XA+0.000
500.0nm 0.000Abs
No. Abs. Mg/ml
1 0.000 0.000
2 0.100 0.100
to
go into the continuous measurement interface. (Fig. 4-14)
ENTER
ESC
0.000 0.600
0.600
0.000
r=1.000
C=1.000XA+0.000
500.0nm 0.000Abs
No. Abs. Mg/ml
1 0.000 0.000
2 0.100 0.100
Fig. 4-14
★Pull the unknown sample in the light path, then press
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
to
measure. The result will be displayed on the screen one by one. At
the same time, the data will be saved in the RAM automatically. Total
200 groups of data can be saved.
(6) Load Curve
All the curve equations will be saved in the RAM automatically. If you
want to reload the saved curve, just follow the following steps:
★Move the cursor on “Load Curve”and press
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
to confirm.
Then the system will go into the curve equation record interface.(Fig.
4-15)
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
Fig. 4-15

- 15 -
★Move the cursor on the equation that you need and press
ESC
ENTER
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
0.000 0.800
28.00
0.000
r=1.000
C=1.852A+2.010
to
confirm. Then the corresponding curve will display on the screen.
(Fig. 4-16)
ESC
ENTER
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
0.000 0.800
28.00
0.000
r=1.000
C=1.852A+2.010
Fig. 4-16
★Press
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
to go into sample continuous measure interface and
measure samples. (Fig. 4-14)
Note: When you reload the curves, the wavelength will be set at the
point where you creat the Standard Curve automatically.
(7) Delete Curve
★Move the cursor on “Delete Curve”and press
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
to confirm.
Then the system goes into the curve equation choosing interface. (Fig.
4-17)
Standard Curve
ESC
ENTER
Create Curve
Load Curve
Delete Curve
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
Fig. 4-17
★Move the cursor on the curve equation that you don’t need again
and press
ESC
ENTER
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
Delete Curve
YES
NO
, then system will ask you to confirm again.(Fig.
4-18)
ESC
ENTER
No. Curve Data
1 C=1.852A+2.010
2 C=1.000A+0.000
Delete Curve
YES
NO
Fig. 4-18
★Move the cursor on “Yes”and press
√Quantitative
Standard Curve
Coefficient
Unit
Coefficient
0.000 Abs
0.000 mg/ml
500.0nm
ESC
ENTER Coefficient
ESC
SET
Coefficient K
Coefficient B
to confirm, then the
curve equation will be deleted. If you don’t want to delete it, just
choose “NO”or press
√Quantitative
Standard Curve
Coefficient
Unit
Coefficient
0.000 Abs
0.000 mg/ml
500.0nm
ESC
ENTER Coefficient
ESC
SET
Coefficient K
Coefficient B
to return.

- 16 -
II Coefficient Method：
(1) Go into “Coefficient”method.
Press Arrow Key to choose “Coefficient”, press
√Quantitative
Standard Curve
Coefficient
Unit
Coefficient
0.000 Abs
0.000 mg/ml
500.0nm
ESC
ENTER Coefficient
ESC
SET
Coefficient K
Coefficient B
to go into
pre-test interface, then press
√Quantitative
Standard Curve
Coefficient
Unit
Coefficient
0.000 Abs
0.000 mg/ml
500.0nm
ESC
ENTER Coefficient
ESC
SET
Coefficient K
Coefficient B
to go into the parameter setting
interface.（Fig. 4-19）；
√Quantitative
Standard Curve
Coefficient
Unit
Coefficient
0.000 Abs
0.000 mg/ml
500.0nm
ESC
ENTER Coefficient
ESC
SET
Coefficient K
Coefficient B
Fig. 4-19
(2) Parameter Setting
★Use Arrow Key to move the cursor on “Coefficient K”and press
√Coefficient
Coefficient K
Coefficient B
Coefficient K
C=K x A+B
K=0.000
K=
ESC
ENTER
to go into K setting interface.（Fig. 4-20）； Input K and press
√Coefficient
Coefficient K
Coefficient B
Coefficient K
C=K x A+B
K=0.000
K=
ESC
ENTER
to confirm ，the system will return the former interface
automatically.
√Coefficient
Coefficient K
Coefficient B
Coefficient K
C=K x A+B
K=0.000
K=
ESC
ENTER
Fig. 4-20
★Input the value of B in the same way, press
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm
Quantitative
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
to go back to
the pre-test interface. （Fig. 4-19）
(3) Set Wavelength
Press
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm
Quantitative
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
to go into wavelength setting interface，input the
wavelength value by the Arrow keypad and press
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm
Quantitative
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
to
confirm. (Fig. 4-21）

- 17 -
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm
Quantitative
WL=500.0nm
Please Input WL.
WL=
ESC
GOTOλ
Fig. 4-21
(4) Calibrate 100%T/0Abs
Press
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm ESC
START
STOP 500.0nm 0.000Abs
No. Abs. Mg/ml
to go into continuous measurement interface (Fig.
4-22). Pull the Reference Solution in the light path，then press
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm ESC
START
STOP 500.0nm 0.000Abs
No. Abs. Mg/ml
to calibrate 100%T/0Abs；
Coefficient
0.000 Abs
0.000 mg/ml
500.0 nm ESC
START
STOP 500.0nm 0.000Abs
No. Abs. Mg/ml
Fig. 4-22
(5) Measurement
Place the samples to be measured in the light path, press
Basic Mode
Quantitative
Setup 08:08
08/16 ESC
ENTER √Setup
D2 Lamp On
W Lamp On
Date & Time
to
measure，then the result will display on the screen . Repeat this step
to measure other samples. All the test results will be listed on the
screen and stored in the RAM automatically. They can be reload even
after a sudden power cut.
Step 4: Print the test result
In the continuous measurement interface, press
Basic Mode
Quantitative
Setup 08:08
08/16 ESC
ENTER √Setup
D2 Lamp On
W Lamp On
Date & Time
to print the test
result.
Step 5: Delete the test data
In the continuous measurement interface, press
Basic Mode
Quantitative
Setup 08:08
08/16 ESC
ENTER √Setup
D2 Lamp On
W Lamp On
Date & Time
to delete the test
result.
4.3 SETUP
In the main menu, press Arrow Key to choose “Setup”and press
Basic Mode
Quantitative
Setup 08:08
08/16 ESC
ENTER √Setup
D2 Lamp On
W Lamp On
Date & Time
to go
into utility setting interface（Fig. 4-23）