Drawell DV-8000 Series User manual

USER’S MANUAL
For DV-8000 Series
Spectrophotometers
Please read operating manual before installation and operation.
Drawell International Technology Limited
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1506,Lane581 XiuChuan Rd.,PuDong New Area,Shanghai,China
Tel: [phone]
Fax: [phone]
Web : www.drawell.com.cn
Email : [email protected]

DV-8000 USER’S MANUAL
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Contents
Safety ……….………………………………………………………………… 3
General ………………………………………………………………………. 3
Electrical …………………………………………………………………….. 3
Warning ………………………………………………………………………… 3
Working Principle ………………………………………………………… 4
Unpacking Instructions …………………………………………………… 4
Specifications ……………………………………………..……………… 5
Installation …………………………………………………………………… 5
Introduction……………………………………………………..……………… 6
Description of keys and operating method………………….….………… 7
Operating Procedures………………………………….……………………… 11
Maintenance……………………………………………………..….…………… 11
Calibration and trouble shooting…………………………………………… 12
Replace W lamp……………………………….………………….. 12
Calibration of Wavelength Accuracy………………………..… 13
Trouble Shooting………………………………………….….…… 14

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Safety:
The safety statements in this manual comply with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its accessories. The
DV-8000 should be operated by appropriate laboratory technicians.
General:
The apparatus described in this manual is designed to be used by properly trained
personnel in a suitable equipped laboratory. For the correct and safe use of this
apparatus it is essential that laboratory personnel follow generally accepted safe
procedures in addition to the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit.
For DV-8000, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometer are corrosive and/or inflammable and
samples may be radioactive, toxic, or potentially infective. Care should be taken to
follow the normal laboratory procedures for handling chemicals and samples.
Electrical:
The power cord shall be inserted in a socket provided with a protective earth contact.
The protective action must not be negated by the use of an extension cord without a
protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus dangerous.
Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
NEVER touch or handle the power supply on DV-8000 due to the high voltage!
The protection is likely to be impaired if, for example, the apparatus
Shows visible damage
Fails to perform the intended measurements
Has been subjected to prolonged storage under unfavorable conditions
Has been subjected to severe transport stresses

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Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps to supply the
light; 2) A Mono-chromator to isolate the wavelength of interest and eliminate the
unwanted second order radiation; 3) A sample compartment to accommodate the sample
solution; 4) A detector to receive the transmitted light and convert it to an electrical signal;
and 5) A digital display to indicate absorbance or transmittance. The block diagram
below illustrates the relationship between these parts.
Block diagram for the Spectrophotometer
Light Mono- Sample Detector Display
Source chromator Compartment
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
mono-chromator where the collimating mirror directs the beam onto the grating. The
grating disperses the light beam to produce the spectrum, a portion of which is focused on
the exit slit of the mono-chromator by a collimating mirror. From here the beam is
passed to a sample compartment through one of the filters, which helps to eliminate
unwanted second order radiation from the diffraction grating. Upon leaving the sample
compartment, the beam is passed to the silicon photodiode detector and causes the
detector to produce an electrical signal that is displayed on the digital display.
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer...................................................................... …. 1
Power Cord................................................................................... …. 1
10mm Glass Cuvette......................................................................... 1 Set of 4
Manual........................................................................................... …. 1
Black Block…………………………………………………………. 1
NOTE：If there is any change in this package, an Accessory & Spare Parts List
attached should be referred to as final.
100%T
0 Abs

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Specifications:
Installation:
1. After carefully unpacking the contents, check the materials with the packing list
(page 2) to ensure that you have received everything in good condition.
2. Place the instrument in a suitable location away from direct sunlight. In order to
have the best performance from your instrument, keep it as far as possible from
any strong magnetic or electrical fields or any electrical device that may generate
high-frequency fields. Set the unit up in an area that is free of dust, corrosive
gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air under and
around the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
5. Turn on your DV-8000 model spectrophotometer. Allow it to warm up for at least
20 minutes before taking any readings.
Model
DV-8000
Wavelength Range
325-1020nm
Spectral Bandwidth
4nm
Optical System
Single Beam, Grating 1200lines/mm
Wavelength Accuracy
±2nm
Wavelength Repeatability
1nm
Photometric Accuracy
±0.5%T
Photometric Repeatability
±0.3%T
Photometric Range
-0.097-1.999A 0-125%T
Stray Light
≤0.2%T @ 340nm
Stability
±0.002A/h @500nm
Display
Graphic LED (4 bits)
Keyboard
Membrane Keypad
Photometric Mode
T, A, C, F
Detector
Silicon Photodiode
Sample Compartment
Standard 10mm path length cuvette
Accommodates 100mm path length cuvette with optional holder
Light Source
Tungsten lamp
Output
RS232 Port
Power Requirement
AC 85-250V
Dimensions (W x D x H)
450 x340 x 180mm
Net Weight
8kg

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Introduction:
Visible spectrophotometer, an analytical instrument commonly used in physic-chemical
laboratories to make quantitative and qualitative analysis of specimen materials in the
near ultraviolet, visible spectral range finds much scope for its service in such fields as
medicine, clinical examination, biochemistry, quality control, petro-chemical industry and
environmental protection.
Description of keys & Operating Methods
The four keys on the keyboard are:
1.MODE 2. PRINT 3. ▽/0%T 4. △/100%T
MODE key used to select the Absorbance、Transmittance、Concentration and Factor
mode.
PRINT key has two functions:
a. To be used in Parallel Port and date transmission (one –way transmission from the
instrument to the Printer).
b. When in Fmode, to confirm the current Fvalue，and then the Mode was switched to
C automatically, and the C value will be calculated (C= F *A ).
▽/0%T key has two functions:
a. To set zero. It is effective only in Tmode. Insert the Black Block into the cuvette
holder, close the sample compartment cover then pull it into the light path. Press
this key, and it will show 0.0.
b. As the ascending key. when in Fmode , press the ▽/0%T key and the Fvalue will
decrease by 1，Holding the key will speed up the decrease. The Minimum Value of
F is 1.

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△/100%T key has the following two functions：
a. When in Aor Tmode, close the sample compartment, press the key and it will read
0.000ABS or 100.0 %T.
b. To be used as the increase key (effective only in F mode). Press this key and the F
value will increase by 1. A long press will speed up the increase. The Maximum
Value of F is 1999.
Example :
Let the F value be 1500.
Method I
a. Press MODE to set it at F mode.
b. If the current F value is 1000 , press the △/100%T key, until it goes up to 1500。
c. Press the PRINT key ，and the current F value shows 1500. Then C mode
automatically appears. Suppose the A value measures 0.125, then the C value
reads 187.5.
Method II
a. Press MODE key to set it at F mode 。
b. If the current F value is 2000 , press the ▽/0%T key，till it reaches 1500. Press
the MODE key again to go to C mode. If the A value measures 0.125, then the C
value reads 187.5.
Operation Procedures
1. Plug the instrument into a grounded outlet
2. Turn the instrument on. Allow the instrument to warm up for at least 20 minutes.
3. Set to desired wavelength with the Wavelength Knob, press the MODE key to
select T/A/C/F.
4. If you want to know T value, press MODE key to change to “T” mode，fill one of
matched cuvettes with Blank Solution.
5. Insert the Black Block into the first cuvette holder, close the sample
compartment cover, set 0.0%T by pressing the ▽/0%T key until display reads
“0.0.
6. Pull the holder to make the Blank in the light path, set 100%T by pressing
the△/100%T key until display reads 100.0.
7. Insert the sample solution to be measured into the cuvette holder. Close the
sample compartment cover. Then pull the sample to be measured into the light
path, read results directly on the digital display.

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Maintenance
1. To ensure the steady operation of the instrument, it is recommended to use an AC
voltage stabilized power source.
2. After operation , turn off the power and cut off the power supply.
3. Put some desiccants over the instrument when it is not in use to keep it clean and
dry.
4. After months of operation or after it is moved, the instrument should be checked for
its wavelength accuracy.
Calibration And Trouble-shooting
After a long time of use, the instrument needs calibrating or repairing, as its
performance index may have changed, here are some tips.
1. Replacement of tungsten-halogen lamp：
1) Turn off and unplug the instrument.
2) Remove the four screws on the sides of the spectrophotometer.
3) Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise.
4) Remove the WL. Setting Knob.
a) Use a Flat Head Screw Driver to pick the cover out from the knob.
b) Use a Spanner or Sleeve to loosen the screw in the knob, then remove the knob.
5) Remove the cover of the instrument very carefully and place it in the back of the
instrument.
BE SURE NOT TO PULL PANEL WIRING LOOSE!
6) Unplug and remove the lamp from ceramic base (the white connector). Insert the
new lamp; pushing it in as far as it will go.
CAUTION:
a) Do not handle the lamp with bare fingers. Use tissue or cloth when handling lamp.
b) There’s no difference in polarity of the two legs of Tungsten-halogen lamp.
7) Set the wavelength of the instrument at 500nm, Switch on the power, move the lamp
up and down and from side to side, until its focus falls exactly on the entrance slit.
Observe the reading in T mode without adjusting the △/100%T key，Readjust the
lamp to make the reading at its maximum. If it can not focus on the center of the
entrance slit, you have to adjust the four screws for the height of the light beam and
the horizontal adjustment.
8) Switch the instrument off, tighten the two screws. Reinstall the instrument cover. Be
sure to prevent all wires from being pinched in the process.
9) Reinstall the four screws. Reposition the Cuvette Holder Control Knob and the
Wavelength Setting Knob.
2. Calibration of wavelength accuracy
DV-8000 spectrophotometer is checked for its wavelength accuracy by a
point-to-point method against the two characteristic absorption peaks of a didymium
filter---529nm and 808nm.

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In case the point-to–point measurement shows a wavelength different from the peak
wavelength of the didymium filter (maximum permissible error is ±2nm), remove the
wavelength knob, loosen the three positioning screws on the wavelength dial, adjust
the dial pointer to the characteristic absorption peak wavelength value, and tighten the
screws。
① ② ③
3. Troubleshooting
Troubles
Causes
Remedies
1. No functioning after
power on.
1.Powersupply not connected
2.Power fuse melted
3.Loose contact of power
switch.
1. Check power line.
2. Replace fuse.
3. Replace power switch.
2.Unstable display
1.Insufficient warm-up time
2. Excessive vibration, strong air
current near light source or
strong external light.
3. Unstable voltage.
4. Poor grounding.
1. Ensure 20 mins warm-up
time.
2.Improve working
conditions
3. Use a Voltage Stabilizer.
4.Check ground connection.
3.Unable to set zero
1.black block in the light path
2.Amplifier failure.
1. Remove the block.
2. Repair amplifier.
4.Unable to set 100%T
1.Tungsten-halogen lamp not lit.
2. Inaccurate light path.
3. Amplifier failure.
1. Check or repair lamp
power circuit.
2. Readjust light path.
3. Repair or replace
amplifier.
5.Concentration
incorrectly calculated
1. Display board out of order.
1. Repair or replace display
board.

Drawell International Technology Limited
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1506,Lane581 XiuChuan Rd.,PuDong New Area,Shanghai,China
Tel: [phone]
Fax: [phone]
Web : www.drawell.com.cn
Email : [e-mail]