Drawell DU-8800DS Series User manual

USER’S MANUAL
For DU-8800DS/RS Series
Spectrophotometers
Drawell Scientific

Contents
Safety ……..……………………………………………………………………. 1
General ………………………………………………………………………. 1
Electrical …………………………………………………………………….. 1
Warning ………………………………………………………………………….. 1
Performance ………………………………………………………………… 2
Radio Interference ………………………………………………………….. 2
Introduction ………………………………………………………………… 2
Working Principle ………………………………………………………….. 3
Unpacking Instructions ……………………………………………………. 4
Specifications ……………………………………………..………………… 4
Installation ……………………………………………………………………… .4
Operation ………………………………………………………..……….…………5
Prepare the Spectrophotometer …………………………………………… 5
Description of keys ………… ……………………………………… 5
Turn on spectrophotometer … …………………………………………….. 6
Basic operation………………………………………………………………. 7
Analyse Sample ………………………………………………………………..12
Basic Mode …………………………………………………………….........12
Quantitative ………………………………………………………………. 15
WL Scan ………………………………………………………………..........20
Kinetics …….………………………………………………………………….. 25
DNA/Protein …………………………………………………………………28
Multi Wavelength ………………………………………………………………30
Setting and Calibration …………………………………………………….. 33
Utility ………………………………………………………………………… 33
Instrument Maintenance….…………………………………………………… 43
Daily Maintenance………………………………………………………….. 43
Trouble Shooting…………………………………………………………… 43
Spare parts Replacement……………………………………..……………44
Appendix A ……………………………………………………………… 48
Appendix B ……………………………………………………………….49

1
Safety:
The safety statements in this manual comply with the requirements of the
HEALTH AND SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its
accessories. This instrument should be operated by appropriate laboratory
technicians.
General:
The apparatus described in this manual is designed to be used by properly
trained personnel in a suitable equipped laboratory. For the correct and safe
use of this apparatus it is essential that laboratory personnel follow generally
accepted safe procedures in addition to the safety precautions called for in this
manual.
The covers on this instrument may be removed for servicing. However, the
inside of the power supply unit is a hazardous area and its cover should not be
removed under any circumstances. There are no serviceable components
inside this power supply unit. For this instrument, avoid touching the high
voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or
inflammable and samples may be radioactive, toxic, or potentially infective.
Care should be taken to follow the normal laboratory procedures for handling
chemicals and samples.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the
local power supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth
contact. The protective action must not be negated by the use of an
extension cord without a protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall
be made inoperative and be secured against any unintended operation.
Note：NEVER touch or handle the power supply on this instrument due to the
high voltage！
The protection is likely to be impaired if, for example, the apparatus
Shows visible damage
Fails to perform the intended measurements

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Has been subjected to prolonged storage under unfavorable conditions
Has been subjected to severe transport stresses
Performance:
To ensure that the instrument is working within its specification, especially
when making measurements of an important nature，carry out performance
checks with particular reference to wavelength and absorbance accuracy.
Performance checks are detailed in this manual.
Radio Interference:
For compliance with the EMC standards referred to in the EC Declaration of
Conformity, it is necessary that only shielded cables supplied by us are used
when connecting the instrument to computers and accessories.
Introduction:
This instrument (Fig 1) is a double beam, general purpose instrument
designed to meet the needs of the Conventional Laboratory, This instrument is
ideal for various applications, such as: Chemistry, Biochemistry,
petrochemistry, Environmental Protection, Food and Beverage Labs, Water
and Waste Water Labs and other fields of quality control and research.
This instrument incorporates a 320×240 dot matrix LCD display for
photometric results, easy operation and wavelength range of 190nm to
1100nm. This instrument is ideal for measurements in the visible and
ultraviolet wavelength region of the electromagnetic spectrum.

3
Fig1
Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Monochromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) Two detectors to receive the
transmitted light and convert it to an electrical signal; and 5) A digital display to
indicate absorbance or transmittance. The block diagram (Fig 2) below
illustrates the relationship between these parts.
Block diagram for the Spectrophotometer
Fig 2
In your spectrophotometer, light from the lamp is focused on the entrance slit
of the monochromator where the collimating mirror directs the beam onto the
grating. The grating disperses the light beam to produce the spectrum, a
portion of which is focused on the exit slit of the monochromator by a
collimating mirror. From here the beam is passed to a sample compartment
through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment,
the beam is passed to the silicon photodiode detector and causes the detector
to produce an electrical signal that is displayed on the digital display.
Power switch Fuse
Fan
Power socket USB Port Parallel Port

4
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following
packing list to ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer........................................................ 1
Mains Lead...................................................................... 1
Glass Cuvettes………………………………………… 1 Set of 4
Quartz Cuvettes………………………………………. 1 Set of 2
Operation Manual........................................................... 1
Software Manual……………………………………… 1
Software Kit (Disc 1+ USB Lead 1+ Dongle 1)……. 1
Fuse……………………………………………………. 2
Note: The printer and auto-cell holder mentioned in this manual are all optional
accessories, they do not come standard with the instrument.
Specifications:
Wavelength Range: 190-1100nm
Spectral Bandpass: 0.5/1/2/4/5nm
Wavelength Accuracy: ±0.3nm
Wavelength Repeatability: 0.2nm
Baseline Flatness. ± 0.001A
Stray Light : 0.05%@220nm&360nm
Photometric Range: 0-200%T,-0.3-3.0A
Noise: 0.001A @ 500nm
Drift: < 0.002A/h @ 500nm
Power Requirements: AC90-250V, 50/60Hz
Dimensions(mm) 640L×530D×270H
Light Source: Tungsten Halogen/Deuterium
Net Weight: 26kg
Installation:
1. After carefully unpacking the contents, check the materials with the
packing list (page 4) to ensure that you have received everything in
good condition.
2. Place the instrument in a suitable location away from direct sunlight.
In order to have the best performance from your instrument, keep it as
far as possible from any strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields. Set the unit
up in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air
under and around the instrument.

5
4. Use the appropriate power cord and plug into a grounded outlet.
5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes
before taking any readings. We suggest you then do the Calibrate
System with the Search 656.1nm to set the wavelength to the
deuterium lamp emission line.
NOTE:
This symbol means Caution, Risk of Danger.
Operation:
Prepare the spectrophotometer
Fig 3 is the control panel. User can perform all operations by pressing the
keys and all the results and operation information are displayed on the LCD.
Fig 3
Description of keys
【LOAD】Load data or curve saved before;

6
【SAVE】Save data or curve;
【SETλ】Set wavelength;
【ZERO】Blank or scan the user base line;
【PRINT】Print test results or screen
【START】Start testing or scanning sample;
【ESC/STOP】Exit to previous screen or cancel the operation;
【ENTER】Confirm the inputted data or selected item; Go into next
setup or screen;
【F1】-【F4】Function based on the information on the screen;
【0】-【9】Input number or letter, consecutively press a numeric key
to select a character;
【+/-/.】Input +,- or dot;
【CE】Clear all characters when you are inputting or clear curve
displays on the screen;
【＜】,【＞】Change “x” scale; Search point after scan; 【＜】clear a
character;
【∧】,【∨】Change “y” scale; Search peak after scan; Scroll items for
selecting; Change capital/small letter last typed in; Browse
the items for selection;
【CELL】Set cell position (Only available when Auto Changer used).
【HELP】Reserved key for future Function Extending, not available now.
Turn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO)(see Fig1). The
instrument starts to initiate and the steps are as below:
1.The instrument will check memory first (Fig 4), please wait or press any
key to skip this step ,after positioning filter, auto-cell changer(if installed) and
D2/W lamps, the screen display as Fig 4A. 15 minutes pass or press【ESC】,
the screen display as Fig 5,Select “No” to skip to main menu( Fig 7) and select
“Yes”(recommended) to calibrate system (Fig 6).The calibrating process
include “get dark current”, “searching 656.1nm” and “check energy”. After
finish the calibration system, go to main menu too (Fig 7).
2. If the data in memory has been lost, the instrument will directly
calibrate system without any choice for you.
3. If no auto-cell changer installed “cell #1” will disappear in Fig7
Fig 4

7
Fig 4A
Fig 5
Fig 6
Fig 7
Basic operation
※Blank
There is a system baseline stored in the memory of the instrument. Usually
user may not rebuild system baseline before test. Only putting the sample into
the sample light path and the reference into the Reference Light Path, the
result can be obtained. As the system baseline always get a little change after

8
the instrument is powered on, it is necessary for the user to rebuild the system
baseline. There are a couple of ways to rebuild the system baseline. Select
“Yes” in Fig 5 or Press【0】In Fig 73 or Press【F4】In Fig41,
Regarding Blanking, important points list below:
A. Take measure in Basic Mode
a. Put the reference cuvette with reference solution into the Reference
Light Path and the sample cuvette with reference solution into the
Sample Light Path. Press the key 【ZERO】for blanking.
Note 1. If the reference solution is too thick, “Energy Low…”will appear
following the “Blanking…”on the screen (Fig 8).If “Energy too Low…” appears
following the “Blanking…”,the test will be paused and “Warning…”will appear
on the screen.(Fig 9).
2. If no automatic changer installed “cell #1” and “Max E” will disappear in
Fig8
Fig 8
3. DO NOT OPEN SAMPLE COMPARTMENT LID DURING
BLANKING.
4. The dark current don’t be taken after power on if you bypass the
calibrating system. It is recommended to take the dark current after
warm up.(See page 38).
Fig 9
b. Take out the sample cuvette, replace the reference solution with
sample solution after flushing the cuvette completely.Put the sample cuvette
into the Sample Light Path, then the result will display on the screen

9
automatically. However the【START】must be pressed in other measurements
such as DNA/Protein, Muli WL and Quantitative etc.
B. Take measure in WL Scan
a. After all scan parameters are entered, put the reference cuvette with
reference solution into the Reference Light Path and the sample cuvette with
sample solution into the sample light path,Press 【START】to scan.
b. (Recommended) After all scan parameters are entered, put the
reference cuvette with reference solution into the Reference Light Path and the
sample cuvette with reference solution into the Sample Light Path, Press
【ZERO】to obtain the user baseline.Then take out the sample cuvette,
replace the reference solution with sample solution after flushing the cuvette
completely. Put the sample cuvette into the Sample Light Path. Press
【START】to scan.
※Set wavelength (Example: set wavelength in “Basic mode”)
Press【SETλ】(Fig 10).
Fig 10
Use numeric keypad to input wavelength (Fig 11).
Fig 11
Press 【ENTER】to change the wavelength from 656.1nm to
450.0nm,and then blank; After blanking, the screen displays as Fig
12.

10
Fig 12
※Load or delete data or curve (Take the “WL scan” test For example)
Press 【3】in Fig.7 go into “WL scan”. After【LOAD】being pressed,
the first file (ABC.wav)in memory will appear on the bottom line of
screen .Showed as Fig 13. Press 【∧】or【∨】to browse the files stroed
in memory. Then if :
1. The key【ENTER】be pressed, the file selected will be loaded and
displays on the screen. Fig 14.
Note (1) The file selected must match “WL scan” test’s type. If not ， the
“file type error…” will appear on the Right of top line.
(2) Different test has different file type. Refer to table 1 on Page 12.
2. The key 【CE】be pressed, the file selected will be deleted by
selecting ”Yes”.
Fig 13
Fig 14

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Table 1
Test File Type
Quantitative Curve ***.fit
Quantitative Test Result ***.
q
ua
WL Scan ***.wav
Kinetics ***.kin
DNA/Protein ***.dna
Multi WL ***.mul
WL Validit
y
***.wlv
Accu. Validity ***.phv
※Save data or curve (Example: Save curve in “WL scan”)
Press the key 【SAVE】in Fig14 to save curve.
Name the curve by pressing the numeric keypad (Fig 15), press
the key【ENTER】to confirm.
.Note(1). Pressing numeric key continually to scroll characters
and pressing 【∧】,【∨】to alter capital letter to
miniscule. Table 2 shows all characters built in.
(2) If the name already exists in memory, the warning
“duplicated name, are you sure ？ ” will appear .
“Yes”for overwrite and“No”for Exit.
(3) The length of filename is less than 4.
Fig 15
Table 2
ke
y
representin
g
ke
y
representin
g
ke
y
representin
g
0 0,+,-,* ,/ 1 1,#,?,:,I 2 2,A,B,C,=
3 3,D,E,F,% 4 4,G,H,I,{5 5,J,K,L,}
6 6,M,N,O,~7 7,P,Q,R,S, 8 8,T,U,V,“
9 9,W,X,Y,Z +/-/. -,.,
※Print test report (For example: Print the report in “Basic mode”,Fig16)
Press the key【PRINT】to print the report (curve or data you have loaded or
tested, Fig 17).

12
Fig 16
Fig 17
Before measurement
Make a blank reference solution by filling a clean cuvette (or test tube) half
full with distilled or de-ionized water or other specified solvent. Wipe the
cuvette with tissue to remove the fingerprints and droplets of liquid.
Analyze Sample
For different user’s requirements, we provide different test methods.
Basic Mode
Push the blank cuvette into the Reference Light Path and Main Light Path. In
main menu (Fig7),press【1】to enter “Basic mode” test . After automatically
blanking, it will display as Fig 18 (automatic changer installed) or Fig 19
( automatic changer uninstalled) and wait for the operator. 【ESC/STOP】to
exit.
Note: If no automatic changer installed “cell #1” and “Max E” will disappear
in Fig18
Fig18

13
Fig19
Test
There are three modes (T%, Abs, conc/factor) for you to select by pressing
【F2】to make choice.
Fig 20
1. Abs mode
Push the blank cuvette into the Reference Light Path and Main Light Path.
Press 【F2】to select Abs mode ,Press 【ZERO】for Blanking , and then
Push the sample into Main Light Path to take reading(Fig 20)
2. T% mode
The operation is the same as Abs test mode but pressing【F2】to select
T% mode .
3. Conc/Factor mode
Press 【F1】to select a concentration unit (Fig 21). If no unit is
suitable for your test, please select the item “Other”, press【ENTER】and
input a new unit by pressing the numeric keypad (Fig 22).
Fig 21

14
Fig 22
4. Push the blank cuvette into the Reference Light Path and Main Light
Path and press 【ZERO】for Blanking. There are now two choices for
you to take:
4.1 Press【F3】to input known F value, Fig 23. Then push the sample into
Main Light Path to take reading of concentration
4.2 Push sample of known concentration into the Main Light Path
Press【F4】to input known Conc value, Fig 24. Then push the sample
into Main Light Path to take reading of concentration.
Note:1.You can select wavelength at any time by pressing
【
SET
λ】.
After your selection, instrument always blanks automatically.
2.If F value is more than 9999,the clue of “out of range” will display
on screen.
Fig 23
Fig 24

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Print Test Report
Press 【PRINT】to print test results (Fig 25).
Fig 25
Quantitative
Press【2】in Main Menu for “Quantitative” Test (Fig 26). Press【ESC/STOP】
to exit.
Note: .If no automatic changer installed “cell #1” will disappear in Fig26.
Fig 26
How to operate
1. Press 【F1】to select unit of concentration (Fig 27).
Fig 27
2. Press【SETλ】to select correction methods and enter the wavelength.
There are three correction methods (single, Isoabsorbance and 3 point,
Fig 28)
Note: Please refer to the Appendix B for the correction method.

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Fig 28
3. Press 【F2】in Fig 26 for more items to select .See Fig 29.
Fig 29
3.1 Press 【F1】in Fig 29 to select fitting method. There are 4 methods
for you to choose: Linear fit, linear fit through zero, square fit and
cubic fit.
3.2Press 【F2】in Fig 29 to enter directly a known standard
curve.Fig29A.
Fig 29A
The constants to be entered depend on which fitting method selected. The table
below lists their relation:
Fittin
g
Method Fittin
g
Equation constants
linear fit through zero C=K1×AK1, r*
Linear fit C=K0+K1×AK0,K1,r*
square fit C=K0+K1×A+K2×A2K0,K1,K2
cubic fit C=K0+K1×A+K2×A2+K3×A3K0,K1,K2,K3
* r : regression co-efficients, default=1