Drawell DU-8800DS Series User manual

USER’S MANUAL
For DU-8800DS/RS Series
Spectrophotometers
Drawell Scientific

Contents
Safety ……..……………………………………………………………………. 1
General ………………………………………………………………………. 1
Electrical …………………………………………………………………….. 1
Warning ………………………………………………………………………….. 1
Performance ………………………………………………………………… 2
Radio Interference ………………………………………………………….. 2
Introduction ………………………………………………………………… 2
Working Principle ………………………………………………………….. 3
Unpacking Instructions ……………………………………………………. 4
Specifications ……………………………………………..………………… 4
Installation ……………………………………………………………………… .4
Operation ………………………………………………………..……….…………5
Prepare the Spectrophotometer …………………………………………… 5
Description of keys ………… ……………………………………… 5
Turn on spectrophotometer … …………………………………………….. 6
Basic operation………………………………………………………………. 7
Analyse Sample ………………………………………………………………..12
Basic Mode …………………………………………………………….........12
Quantitative ………………………………………………………………. 15
WL Scan ………………………………………………………………..........20
Kinetics …….………………………………………………………………….. 25
DNA/Protein …………………………………………………………………28
Multi Wavelength ………………………………………………………………30
Setting and Calibration …………………………………………………….. 33
Utility ………………………………………………………………………… 33
Instrument Maintenance….…………………………………………………… 43
Daily Maintenance………………………………………………………….. 43
Trouble Shooting…………………………………………………………… 43
Spare parts Replacement……………………………………..……………44
Appendix A ……………………………………………………………… 48
Appendix B ……………………………………………………………….49

1
Safety:
The safety statements in this manual comply with the requirements of the
HEALTH AND SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its
accessories. This instrument should be operated by appropriate laboratory
technicians.
General:
The apparatus described in this manual is designed to be used by properly
trained personnel in a suitable equipped laboratory. For the correct and safe
use of this apparatus it is essential that laboratory personnel follow generally
accepted safe procedures in addition to the safety precautions called for in this
manual.
The covers on this instrument may be removed for servicing. However, the
inside of the power supply unit is a hazardous area and its cover should not be
removed under any circumstances. There are no serviceable components
inside this power supply unit. For this instrument, avoid touching the high
voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or
inflammable and samples may be radioactive, toxic, or potentially infective.
Care should be taken to follow the normal laboratory procedures for handling
chemicals and samples.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the
local power supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth
contact. The protective action must not be negated by the use of an
extension cord without a protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall
be made inoperative and be secured against any unintended operation.
Note：NEVER touch or handle the power supply on this instrument due to the
high voltage！
The protection is likely to be impaired if, for example, the apparatus
Shows visible damage
Fails to perform the intended measurements

2
Has been subjected to prolonged storage under unfavorable conditions
Has been subjected to severe transport stresses
Performance:
To ensure that the instrument is working within its specification, especially
when making measurements of an important nature，carry out performance
checks with particular reference to wavelength and absorbance accuracy.
Performance checks are detailed in this manual.
Radio Interference:
For compliance with the EMC standards referred to in the EC Declaration of
Conformity, it is necessary that only shielded cables supplied by us are used
when connecting the instrument to computers and accessories.
Introduction:
This instrument (Fig 1) is a double beam, general purpose instrument
designed to meet the needs of the Conventional Laboratory, This instrument is
ideal for various applications, such as: Chemistry, Biochemistry,
petrochemistry, Environmental Protection, Food and Beverage Labs, Water
and Waste Water Labs and other fields of quality control and research.
This instrument incorporates a 320×240 dot matrix LCD display for
photometric results, easy operation and wavelength range of 190nm to
1100nm. This instrument is ideal for measurements in the visible and
ultraviolet wavelength region of the electromagnetic spectrum.

3
Fig1
Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Monochromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) Two detectors to receive the
transmitted light and convert it to an electrical signal; and 5) A digital display to
indicate absorbance or transmittance. The block diagram (Fig 2) below
illustrates the relationship between these parts.
Block diagram for the Spectrophotometer
Fig 2
In your spectrophotometer, light from the lamp is focused on the entrance slit
of the monochromator where the collimating mirror directs the beam onto the
grating. The grating disperses the light beam to produce the spectrum, a
portion of which is focused on the exit slit of the monochromator by a
collimating mirror. From here the beam is passed to a sample compartment
through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment,
the beam is passed to the silicon photodiode detector and causes the detector
to produce an electrical signal that is displayed on the digital display.
Power switch Fuse
Fan
Power socket USB Port Parallel Port

4
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following
packing list to ensure that you have received everything in good condition.
Packing List
Description Quantity
Spectrophotometer........................................................ 1
Mains Lead...................................................................... 1
Glass Cuvettes………………………………………… 1 Set of 4
Quartz Cuvettes………………………………………. 1 Set of 2
Operation Manual........................................................... 1
Software Manual……………………………………… 1
Software Kit (Disc 1+ USB Lead 1+ Dongle 1)……. 1
Fuse……………………………………………………. 2
Note: The printer and auto-cell holder mentioned in this manual are all optional
accessories, they do not come standard with the instrument.
Specifications:
Wavelength Range: 190-1100nm
Spectral Bandpass: 0.5/1/2/4/5nm
Wavelength Accuracy: ±0.3nm
Wavelength Repeatability: 0.2nm
Baseline Flatness. ± 0.001A
Stray Light : 0.05%@220nm&360nm
Photometric Range: 0-200%T,-0.3-3.0A
Noise: 0.001A @ 500nm
Drift: < 0.002A/h @ 500nm
Power Requirements: AC90-250V, 50/60Hz
Dimensions(mm) 640L×530D×270H
Light Source: Tungsten Halogen/Deuterium
Net Weight: 26kg
Installation:
1. After carefully unpacking the contents, check the materials with the
packing list (page 4) to ensure that you have received everything in
good condition.
2. Place the instrument in a suitable location away from direct sunlight.
In order to have the best performance from your instrument, keep it as
far as possible from any strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields. Set the unit
up in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air
under and around the instrument.

5
4. Use the appropriate power cord and plug into a grounded outlet.
5. Turn on your spectrophotometer. Allow it to warm up for 15 minutes
before taking any readings. We suggest you then do the Calibrate
System with the Search 656.1nm to set the wavelength to the
deuterium lamp emission line.
NOTE:
This symbol means Caution, Risk of Danger.
Operation:
Prepare the spectrophotometer
Fig 3 is the control panel. User can perform all operations by pressing the
keys and all the results and operation information are displayed on the LCD.
Fig 3
Description of keys
【LOAD】Load data or curve saved before;

6
【SAVE】Save data or curve;
【SETλ】Set wavelength;
【ZERO】Blank or scan the user base line;
【PRINT】Print test results or screen
【START】Start testing or scanning sample;
【ESC/STOP】Exit to previous screen or cancel the operation;
【ENTER】Confirm the inputted data or selected item; Go into next
setup or screen;
【F1】-【F4】Function based on the information on the screen;
【0】-【9】Input number or letter, consecutively press a numeric key
to select a character;
【+/-/.】Input +,- or dot;
【CE】Clear all characters when you are inputting or clear curve
displays on the screen;
【＜】,【＞】Change “x” scale; Search point after scan; 【＜】clear a
character;
【∧】,【∨】Change “y” scale; Search peak after scan; Scroll items for
selecting; Change capital/small letter last typed in; Browse
the items for selection;
【CELL】Set cell position (Only available when Auto Changer used).
【HELP】Reserved key for future Function Extending, not available now.
Turn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO)(see Fig1). The
instrument starts to initiate and the steps are as below:
1.The instrument will check memory first (Fig 4), please wait or press any
key to skip this step ,after positioning filter, auto-cell changer(if installed) and
D2/W lamps, the screen display as Fig 4A. 15 minutes pass or press【ESC】,
the screen display as Fig 5,Select “No” to skip to main menu( Fig 7) and select
“Yes”(recommended) to calibrate system (Fig 6).The calibrating process
include “get dark current”, “searching 656.1nm” and “check energy”. After
finish the calibration system, go to main menu too (Fig 7).
2. If the data in memory has been lost, the instrument will directly
calibrate system without any choice for you.
3. If no auto-cell changer installed “cell #1” will disappear in Fig7
Fig 4