Esco CelCulture CO2 User manual

CelCulture
User
Manual
Thank you for purchasing this Esco CO2Incubator. Please read
this manual thoroughly to familiarize yourself with the many
unique features and exciting innovations we have built into
your new equipment. Esco provides many other resources at
our website, www.escoglobal.com, to complement this manual
and help you enjoy many years of productive and safe use of
your Esco products.
Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USA
Toll-Free USA and Canada 888-375-ESCO
Tel [phone] • Fax [phone]
us.escoglobal.com • [e-mail]
Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel [phone] • Fax [phone]
www.escoglobal.com • [email protected]

Copyright Information
© Copyright 2011 Esco Micro Pte. Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and all rights are
reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify any
person or entity of such change.
Celculture® is a registered trademark of Esco.
“Material in this manual is provided for informational purposes only. The contents and the product described in
this manual (including any appendix, addendum, attachment or inclusion), are subject to change without
notice. Esco makes no representations or warranties as to the accuracy of the information contained in this
manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related
to the use of this manual.”

CO2Incubator
i
Introductory Page
i
Table of Contents
iii
Warranty Terms and Conditions
v
Introduction
v
1. Products Covered
v
2. Safety Warning
v
3. Limitation of Liability
v
4. European Union Directives on WEEE and RoHS
vii
Declaration of Conformity
1
Chapter 1 –Product Information
1
1.1 About CelCulture CO2Incubator
1
1.2 Labels
2
1.3 Quick View
2
1.3.1 Front Quick View
3
1.3.2 Back Quick View
5
Chapter 2 –Installation
5
2.1 General Requirements
5
2.1.1 Location Requirements
5
2.1.2 Environmental Requirements
6
2.1.3 Power Requirements
6
2.2 Installation
6
2.2.1 Unit Leveling
6
2.2.2 Unit Stacking
5
2.2.3 ULPA Filter Installation
7
2.2.4 Shelves System Installation
7
2.2.5 Connecting the Unit to Electrical Power
7
2.2.6 Connecting the Unit to CO2and N2Gas Supply
7
2.2.7 Connecting Alarm Contacts and RS485 Communication Port
8
2.3 Preliminary Cleaning
8
2.4 Filling the Humidity Pan
8
2.5 Filtered Air Exchange
8
2.6 First Run
9
2.7 Disclaimer
11
Chapter 3 –Control System and Operation
11
3.1 CelCulture Control System
12
3.2 Menu Options
13
3.2.1 Settings
13
3.2.2 Calibration
13
3.2.3 Admin
15
3.2.4 Datalogging
16
3.2.5 Service
16
3.2.6 Help
16
3.3 Calibration
16
3.3.1 Unit Calibration
17
3.3.2 Analog Output Calibration
18
3.4 Diagnostic Readout
19
3.5 Alarm

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21
Chapter 4 –General Maintenance
21
4.1 Scheduled Maintenance
21
4.1.1 Check CO2/N2Gas Tank Level
21
4.1.2 Check Water Level in the Humidity Pan
21
4.1.3 Cleaning and Decontamination
21
4.1.4 General Inspection
21
4.1.5 Calibration of Temperature, CO2, O2and Humidity
21
4.1.6 Change ULPA Filter
21
4.1.7 Change Inline Filter
22
4.2 Maintenance/Service Log
23
Chapter 5 –Cleaning and Decontamination
23
5.1 Cleaning Procedure
23
5.2 Decontamination
23
5.2.1 Decontamination Procedure
25
Chapter 6 –Troubleshooting
29
Chapter 7 –Engineering Details
29
7.1 Engineering Drawing
30
7.2 General Specifications
Appendix

CO2Incubator
iii
Esco products come with either a 1, 2 or 3 year limited warranty, depending on the product purchased,
beginning on the date of shipment from any Esco international warehousing location. To determine which
warranty applies to your product, refer to the appendix below.
Esco's limited warranty covers defects in materials and workmanship. Esco's liability under this limited
warranty shall be, at our option, to repair or replace any defective parts of the equipment, provided if proven
to the satisfaction of Esco that these parts were defective at the time of being sold, and that all defective parts
shall be returned, properly identified with a Return Authorization.
This limited warranty covers parts only, and not transportation/insurance charges.
This limited warranty does not cover:
Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you
must file a claim directly with the freight carrier.
Products with missing or defaced serial numbers.
Products for which Esco has not received payment.
Problems that result from:
oExternal causes such as accident, abuse, misuse, problems with electrical power, improper
operating environmental conditions.
oServicing not authorized by Esco.
oUsage that is not in accordance with product instructions.
oFailure to follow the product instructions.
oFailure to perform preventive maintenance.
oProblems caused by using accessories, parts, or components not supplied by Esco.
oDamage by fire, floods, or acts of God.
oCustomer modifications to the product
Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs.
Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment. If the
objects are highly valuable, user is advised to have in place independent external preventive
measures such as connection to a centralized alarm system.
Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by
the original manufacturer. The customer agrees that in relation to these products purchased through Esco, our
limited warranty shall not apply and the original manufacturer's warranty shall be the sole warranty in respect
of these products. The customer shall utilize that warranty for the support of such products and in any event
not look to Esco for such warranty support.
Esco encourages all users to register their equipment online at www.escoglobal.com/warranty or complete the
warranty registration form included with each product.
ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED
WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR
IMPLIED, WILL APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY
BEYOND THE REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT,
CONSEQUENTIAL OR INCIDENTAL DAMAGES, INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-
PARTY CLAIMS AGAINST YOU FOR DAMAGES, FOR PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST
WORK. ESCO'S LIABILITY WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE
SUBJECT OF A CLAIM. THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE.

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These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and
shall be subject to the exclusive jurisdiction of the courts of Singapore.
Technical Support, Warranty Service Contacts
USA: 1-877-479-3726
Singapore: [phone]
Global Email Helpdesk: supp[email protected]m
Visit http://www.escoglobal.com/ to talk to a Live Support Representative
Distributors are encouraged to visit the Distributor Intranet for self-help materials.
Product Appendix, Warranty Listings
Biological Safety Cabinets, Laminar Flow Cabinets,
HEPA-Filtered Cabinets (except Streamline brand)
The warranty periods for BSC may vary by country. Contact
your local distributor for specific warranty details.
Laboratory Fume Hoods
1 year limited.
Ductless Fume Hoods
3 years limited for Ascent Opti's, 5 years for Ascent Max's.
Cleanroom Equipment
1 year limited.
Laboratory Ovens and Incubators
1 year limited.
CO2Incubators
2 years limited.
Containment/Pharma Products
2 years limited.
Ultralow Temperature Freezer
3 years limited. 60 months on Compressor.
The warranty period starts two months from the date your equipment is shipped from Esco facility for
international distributors. This allows shipping time so the warranty will go into effect at approximately the
same time the equipment is delivered to the user. The warranty protection extends to any subsequent owner
during the warranty period. Distributors who stock Esco equipment are allowed an additional four months for
delivery and installation, providing the product is registered with Esco. User can register product online at
www.escoglobal.com/warranty or complete the warranty registration form included with each product.
Policy updated on 12th Apr 2011 (This limited warranty policy does not apply to products purchased before 12th
Apr 2011)

CO2Incubator
v
1. Products Covered
CelCulture CO2Incubator
Model
Size
Sensor
Control
Filter
Decontamination
Electrical Rating
CCL-170A-8
170 L
TC
CO2
ULPA
90oC Moist Heat
220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170B-8
170 L
IR
CO2
ULPA
90oC Moist Heat
220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170T-8*
170 L
IR
CO2/O2
ULPA
90oC Moist Heat
220 - 240 V, AC, 50/60 Hz, 1Φ
CCL-170A-9
170 L
TC
CO2
ULPA
90oC Moist Heat
110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170B-9
170 L
IR
CO2
ULPA
90oC Moist Heat
110 - 130 V, AC, 50/60 Hz, 1Φ
CCL-170T-9*
170 L
IR
CO2/O2
ULPA
90oC Moist Heat
110 - 130 V, AC, 50/60 Hz, 1Φ
*Please note that these units haven’t been evaluated to UL/CB 61010-1
2. Safety Warning
Anyone working with, on or around this equipment should read this manual. Failure to read,
understand and follow the instructions given in this documentation may result in damage to the unit,
injury to operating personnel, and / or poor equipment performance.
Any adjustment, modification or maintenance to this equipment must be undertaken by qualified
service personnel.
The use of any hazardous materials in this equipment must be monitored by an industrial hygienist,
safety officer or some other suitably qualified individual.
Before you proceed, you should thoroughly understand the installation procedures and take note of
the environmental / electrical requirements.
In this manual, important safety related points will be marked with the symbol.
If the equipment is used in a manner not specified by this manual, the protection provided by this
equipment may be impaired.
3. Limitation of Liability
The disposal and / or emission of substances used in connection with this equipment may be governed by
various local regulations. Familiarization and compliance with any such regulations are the sole responsibility
of the users. Esco’s liability is limited with respect to user compliance with such regulations.
4. European Union Directive on WEEE and RoHS
The European Union has issued two directives:
• Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE)
This product is required to comply with the European Union’s Waste Electrical &
Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following
symbol:
Esco sells products through distributors throughout Europe. Contact your local Esco
distributor for recycling/disposal.
• Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS)
With respect to the directive on RoHS, please note that this freezer falls under category 8 (medical devices)
and category 9 (monitoring and control instruments) and is therefore exempted from requirement to comply
with the provisions of this directive.

CelCulture
vi

CO2Incubator
vii
Declaration of Conformation
In accordance to EN ISO/IEC 17050-1:2010
We, Esco Micro Pte. Ltd.
of 21 Changi South Street 1
Singapore, 486777
Tel: [phone]
Fax: [phone]
declare on our sole responsibility that the product:
Category : CO2Incubator
Brand : CelCulture
Model : CCL-170A-8, CCL-170B-8, CCL-170T-8
in accordance with the following directives:
2006/95/EEC : The Low Voltage Directive and its amending directives
92/31/EEC : The Electromagnetic Compatibility Directive and its amending
directives
has been designed to comply with the requirement of the following Harmonized Standard:
Low Voltage : EN 61010-1:2010
EMC : EN 61326-1:2006 Class B
More information may be obtained from Esco’s authorized distributors located within the European Union. A
list of these parties and their contact information is available on request from Esco.
_______________________________
XQ Lin
Group CEO, Esco
This Declaration of Conformity is only applicable for 230V AC 50Hz units

CelCulture
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1
CO2Incubator
-
1.1 About CelCulture CO2Incubators
A CO2Incubator is a device for controlling the temperature, humidity, CO₂ level, and other conditions in which
a cell culture is being grown or maintained. Incubators are essential for experimental work in cell biology,
microbiology and molecular biology and are widely used in scientific research to grow and maintain cell
cultures. Other typical fields of application include tissue engineering, in vitro fertilization, neuroscience,
cancer research and other cell research.
The most common setting of the CO2Incubators is 37oC temperature, 5% CO2concentration and 90-95%
humidity, for culturing mammalian cells. Mammalian cells have very stringent requirements of the
environment, which can be closely monitored and maintained by the use of CO2Incubators. Other applications
such as hypoxic study or microorganism culture can also be carried out in CO2Incubators by adjusting the
settings of temperature from ambient + 3oC to 60oC, CO2concentration from 0% to 20%, O2concentration 1%
to 20.7% and humidity up to 97%.
Note: Given high-accurate temperature and CO2level measurement and high-precision control, it is especially suitable for
growing mammalian cells at 37oC and in the presence of 5% CO2.
1.2 Labels
Model –model of the unit
Serial –the unit’s serial number
Power –max power requirement and electrical specification
Interior Volume –the unit’s inner chamber volume
Manufactured in … - year of manufacture

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1.3 Quick View
1.3.1 Front Quick View
1. Top Cover
2. Door switch
3. Circulation fan
4. ULPA filter
5. Shelves
6. Sampling port
7. Glass door gasket
8. Humidity water pan
9. Leveling feet
10. Control panel
11. Outer door
12. Glass door
13. Pilaster shelf support
14. Glass door latch
15. Magnetic gasket
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15

3
CO2Incubator
1.3.2 Back Quick View
1. Main switch
2. Power inlet
3. RS485 communication
4. Analog output (optional)
5. Alarm contacts
6. CO2gas inlet
7. CO2gas inlet (CO2backup system)
8. N2gas inlet (for suppressed O2control)
9. N2gas inlet (for suppressed O2control - N2backup system)
10. Access port
1
2
3
4
5
6
7
8
9
10

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5
CO2Incubator
–
2.1 General Requirement
2.1.1 Location Requirements
The CelCulture CO2Incubator can be placed on the floor or working surface or floor stand. Do not
place equipment close to flammable materials or devices that produce excess heat.
Before moving or relocating the unit, make sure to:
oRemove the water in the humidity to avoid spillage.
oTransfer the sample cells to another incubator.
It is essential to ensure adequate air ventilation around the equipment.
When moving the equipment, do not lift using the door or door handle.
The spacing to the side wall should be at least 10 cm (3.9”).
The spacing at the back of the equipment should be at least 11 cm (4.3”).
2.1.2 Environmental Requirements
Indoor use
Altitudes of up to 2000 meters (6600’)
Up to 90% relative humidity –non condensing
Temperature between 18°C - 34°C (64°F - 93°F)
Note: 23°C - 27°C (73°F - 81°F) is the range of the best performance
Must be installed in room with sufficient ventilation. The room ventilation should be a technical
ventilation that is in accordance with the requirements of ZH 1/119 (Guidelines for laboratories) or
some other suited ventilation system with appropriate capacity.

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2.1.3 Power Requirements
The equipment is designed to work with an electrical supply of 220 VAC –240 VAC, 50/60 Hz or
110VAC –130 VAC, 50/60 Hz. If your available electrical supply is not within these parameters, then a
suitable power supply must be used, otherwise damage may be caused to the device or a hazardous
situation may result.
It is recommended that the equipment is connected to a dedicated power source with protective
grounding installed
There should be unobstructed access to the main power plug. The power plug is the main
disconnecting device on the unit
This equipment can sustain a maximum of ±10% nominal voltage fluctuation; otherwise a power
stabilizer is needed.
2.2 Installation
2.2.1 Unit Leveling
It is important that the equipment is properly leveled by positioning a bubble level on the center top shelf of
the incubator. The incubator has a built in leveling feet that can be adjusted to level the unit. Adjust the feet
until the unit sits level left to right and front to back.
2.2.2 Unit Stacking
If equipment is to be stacked on top of each other,
the equipment with the lower working temperature
must always be placed at the bottom.
1. Remove the screws from the back at top
left and top right of the incubator. These
screws will be used to secure the stacking
brackets later.
2. Position a bubble level on the center top
shelf of the incubator and adjust the
leveling feet of the lower incubator until
the incubator is level.
3. Lift the 2nd incubator onto the top the 1st
incubator and align them.
Note: The feet are NOT to be removed and they
should be adjusted to allow 40 mm to 50 mm
(1.5” to 2”) clearance between the incubators.
4. There are 4 pieces provided in the
accessory kit box, 2 for the front and 2 for
the back.
5. Secure the stacking brackets to the cabinet
using the M4 screw provided.
6. Tighten the nut on the foot to secure the
stacking brackets.
2.2.3 ULPA Filter Installation
To install the ULPA filter (2), push it upward in place –just beneath the top
duct (1). To uninstall, pull it downward.
1
2

7
CO2Incubator
2.2.4 Shelves System Installation
Pilasters are held against the chamber walls using thumbscrews.
There are 2 pilaster models –the shelf railing holders in the front
pilasters (2) is open upward, while the one in the back pilasters (1)
are open toward the back of the unit.
Shelf railings (3) are installed by sliding them in the shelf railing
holders in the back pilaster, then the front.
Slide in the shelves (4) in the openings of the shelf railings
2.2.5 Connecting the Unit to Electrical Power
Plug the provided power cord to the power inlet at the rear of the unit. Connect the power cord to the building
outlet and turn ON the power switch of the outlet if necessary.
Note: Refer to the serial number tag located on the right side of the cabinet for electrical specification. Ensure the cabinet is
connected to the correct power source.
2.2.6 Connecting the Unit to CO2and N2Gas Supply
CO2 gas supply cylinder and N₂ gas supply cylinder (for suppressed O2model) should be industrial grade 99.5%
pure and without siphon tube. Install a 2-stage pressure regulator to the tank outlet. The inlet pressure must
be regulated to 15 psig (103.4 kPa).
Use the Connection Hose Kit provided in the Accessories Kit Box. A 0.2 micron in-line filter (1) is
provided to remove any contaminants in the CO2 gas
supply. Check all fittings for leaks.
Connect one end of the tubing to the barb fitting at
the rear of the cabinet labeled CO2 Inlet #1 and install
the clamp (2) to properly secure the tubing. For
Suppressed O2model, connect another tube to the
N2Inlet #1.
Connect the other end of the tubing to the outlet of
the 2-stage pressure regulator and install the clamp
(2).
Note: Make sure that the pressure in the two stage gas is
regulated to 15 psi or 103.4 kPa.
If unit is equipped with a built-in gas backup system, there will be 2 gas inlets. Each of the inlets should be
connected to individual gas tanks as described above.
Note: Consult your facility safety officer to ensure that the equipment is installed in accordance to your local regulations
and code.
2.2.7 Connecting Alarm Contacts and RS485 Communication Port
A set of relay contacts located on the rear of the unit is provided to monitor for
temperature, humidity O2or CO2alarms. The terminals provided on the alarm contact allow
for a NO (normally open) output, a NC (normally closed) and COM (common) connection. In
the event of an alarm condition or power failure, the NO contact will close, and the NC
contact will open. Once the alarm is cleared, the contacts return to their normal condition.
To activate this function, see section 3.3.12. Relay Activation.
The RS485 provides serial communication port for PC. It can be daisy chained from product
to product and connected to PC.
1
2
To unit
To tank
2
1
3
4

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2.3 Preliminary Cleaning
Remove the protective coating on the shelf supports and air duct, if present.
Use a suitable laboratory disinfectant to disinfect all interior surfaces prior to using the product.
Note: Do not use chlorine based cleaner
See section 5.1 Cleaning Procedure for information on cleaning the unit
2.4 Filling the Humidity Pan
For best operation of the incubator, sterilized distilled, de-mineralized or de-ionized water should be used in
the humidity pan.
Chlorinated tap water is not to be used as chlorine can deteriorate the stainless steel. Tap water may also have
a high mineral content, which would produce a build-up of scale in the reservoir. Water should always be
sterilized or treated with a decontaminant, safe for use with stainless steel as well as safe for the product,
prior to being introduced into the humidity pan.
It is recommended to check the water level and refill the humidity pan once a week.
For normal operation, fill up water up to approximately 1000 ml and for decontamination process, fill water
only up to 400 ml. Place the pan directly on the incubator floor to ensure optimum humidity and temperature
response. The pan needs to be inserted completely to the back of the incubator, so that condensed water can
drip back into the pan.
For Suppressed O2model: ensure that the sparger is fully immersed in water.
2.5 Filtered Air Exchange
Filtered air exchange is an intentional ‘leak’ in the chamber to reduce the relative humidity to an acceptable
level and to ensure no condensation occurs within the chamber. A small amount of ambient air is being drawn
from outside of the incubator by the re-circulating fan, through a tubing and 1μm filter to prevent
contamination in the chamber. Air is being ‘pushed out’ through another tubing and 1μm filter installed on the
access port plug located at the back of incubator. Tubing installed inside the chamber for filtered air exchange
is silicon tubing which can withstand high decontamination temperature.
If high humidity level is required, the filtered air exchange can be removed and “Elevated Humidity” can be
activated in the control panel. (Call Esco or your distributor to activate this function)
2.6 First Run
After the incubator has been properly installed, and connected to the power supply, humidity pan filled, and
the unit connected to gas supply, follow the procedures for the unit’s initial start-up.
1. Switch ON the unit. A welcome message will appear on the display. Press SET to continue.
2. Keypad operation will appear on the display. Press SET to continue.
3. Set the date, time, temperature set point, %CO2 set point and %O2 set point:
a. Set Date. The digit will blink. Use UP/DOWN buttons to select the current date and press SET
to confirm each digit.
b. Set Time. The digit will blink. Use UP/DOWN buttons to select the current time and press SET
to confirm each digit.
c. Set Temperature Set Point. Use UP/DOWN buttons to select the temperature set point. Press
SET to confirm
d. Set %CO2 Set Point. Use UP/DOWN buttons to select the %CO2 set point. Press SET to
confirm.

9
CO2Incubator
e. Set %O2 Set Point. Use UP/DOWN buttons to select the %O2 set point. Press SET to confirm.
(For suppressed O2model only)
4. Incubator set-up is finish.
5. The unit will run a memory check.
6. Incubator will do initial start-up and will continue to the main menu.
The outer door has a magnetic closure, which can be opened by pulling on the rim of the door. The inner glass
door has a tight gasket seal, which prevents the chamber from being contaminated and the chamber
environment from being disturbed.
When the main door is opened, the blower fan and gas supply is automatically turned off.
Note: Make sure that the door switch will not be overridden or controlled manually or by any means when the main door is
opened.
Allow 2 hours for the incubator to stabilize before proceeding to calibration.
2.7 Disclaimer
The performance and safety of the incubator, while rigorously evaluated at the factory, cannot be guaranteed
once after transit and installation. Therefore the on-site testing is always recommended.

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