Esco CelCulture User manual

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
1 HM
CelCulture CO2 Incubator ESCO
CCL-170B-8
CelCulture Control System
DECON Button & DECON LED
To activate 90oC moist heat decontamination procedure
During decontamination procedure, the yellow DECON LED will light up
MENU Button
When the main LCD shows normal display –MENU button will activate the
menu options
Within the menu display –MENU button will bring up the previous menu level
SET Button
Within the menu display –SET button will confirm a selection or value
UP/DOWN Buttons
Within the menu display –UP and DOWN buttons will scroll the display up and
down
When an input is required –UP and DOWN buttons will increase and decrease
a value
MUTE Button & ALARM LED
MUTE button will mute the audible alarm for a period of time
The red ALARM LED will light up whenever an alarm condition is triggered

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
2 HM
HEAT LED
HEAT LED will light up whenever a heating process is activated
INJECT LED
INJECT LED will light up whenever a gas injection process is activated
oC/%RH Display
When oC displayed, the display shows the temperature in the chamber
When %RH displayed, the display shows the relative humidity in the chamber
(optional)
%CO2/%O2 Display
When %CO2 displayed, the display shows CO2 concentration level in the
chamber
When %O2 displayed, the display shows O2 concentration level in the chamber
(suppressed O2 model)
Problems / Corrective Actions
Unit will not turn on
Possible Cause
Corrective Actions
Power failure
Is the cord set connected to the power inlet at rear of cabinet as defined
in the installation section of the manual?
Is there power at the electrical outlet which the unit is plugged into?
Use voltmeter or test pen to check power on the electrical socket.
Is the unit’s power switch turned on?
Check whether power cord has been connected properly into the
wall/building socket and also into the incubator.
Check whether the power cord is giving power, measure the AC voltage
between the live and the neutral terminal of the cord by using a

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
3 HM
voltmeter. If voltage is not within +/-2% of the socket voltage, replace the
power cord.
Check the fuse of the power cord.
CO2 level is below or above CO2 set-point
Possible Cause
Corrective Actions
Unit is not connected to the pressure
regulated CO₂ source.
Check gas hose connection at the rear of unit
to the CO2 source as defined in the
installation section of the manual.
CO2 inlet pressure not regulated correctly
The CO2 inlet gas pressure should be
regulated to approximate 15 psig.
CO2 gas tank is empty
Replace CO2 gas tank.
In-line filter is dirty / saturated
Replace in-line filter.
Gas hose is leaking
If hose is leaking, check the pressure regulator
to ensure CO2 inlet pressure is not more than
15 psig. Check for leakage at hose connection.
Gas hose is dirty or obstructed
Check physically for dirt accumulation or
obstruction, clean or replace it.
CO2 set-point was recently lowered or raised
Unit is still not stabilized after the set-point
was changed
Check CO₂ set-point. Allow 2 hours for the
unit to stabilize after adjustment of new set-
point.
If the CO2 set point is lowered, the outer and
inner doors must be opened.

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
4 HM
Inner door opened for an extended period of
time
Close the door and let the unit stabilize.
Access port stopper at the rear of cabinet
was removed or not install
Ensure access port stopper and filter are
installed.
Air leakage through Inner door gasket
Check installation of the gasket ensure it is
properly secure to the cabinet and that there
is no gap.
Check the joining ends of the gasket to make
sure there is no gap. Use silicon sealant to seal
the joins if there is a gap.
Check gasket profile is not deformed or torn.
Otherwise replace new door gasket.
Temperature is above or below temperature set-point
Possible Cause
Corrective Actions
Temperature set-point was recently lowered
or raised. Unit is still not stabilized after set-
point was changed.
Check temperature set-point. Allow 2 hours
for the unit to stabilize after adjustment of
new set-point.
If the temperature was lowered, open the
outer and inner doors.
Lowering the temperature without opening
the doors could lead to condensation.
Check if the glass door latch is properly
closed.
Inner door opened for an extended period of
time.
Close the door and let the unit to stabilize.

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
5 HM
Air leakage through Inner door gasket.
Check installation of the gasket ensure it is
properly secure to the cabinet and that there
is no gap.
Check the joining ends of the gasket to make
there is no gap. Use silicon sealant to seal the
joins if there is gap.
Check gasket profile is not deformed or torn.
Otherwise replace new door gasket.
Humidity level is below standard
humidity level (For units with RH display
options installed)
Possible Cause
Corrective Actions
There is no water or not enough water in
the humidity pan.
Ensure there is enough water in the
humidity pan. For normal operating
temperature, humidity pan should be half
filled.
Cleaning & Decontamination
Cleaning Procedure
1. Transfer all samples to another CO₂ incubator or store them in a safe place.
2. Turn off and unplug the device. If needed; mark that the unit is deactivated or being
serviced.
3. Strip the unit for cleaning:
Remove the ULPA filter. See section 2.2.3 ULPA Filter Installation.
Remove the shelf system. See section 2.2.4 Shelves System Installation.
Remove the sparger and tubing (for suppressed O2 model) and the humidity pan.
4. Start cleaning process:
Thoroughly remove dirt and residues onto the surfaces of the work space and on the
accessories.
Spray disinfectant onto work space surfaces and all dismantled parts or wipe with a
cloth dampened with disinfectant.

CTN
LABORATÓRIO DE ENSAIOS TECNOLÓGICOS EM ÁREAS
LIMPAS
ESTRADA NACIONAL 10,2695-066 BOBADELA
Edição: 01
Revisão: 00
Data: 2014
6 HM
Do not spray disinfectant directly onto the CO₂, O₂ or humidity sensor.
Allow disinfectant to react as specified by manufacturer.
Wipe all surfaces using a moistened clean cloth to remove disinfectant.
Wipe all surfaces thoroughly until dry.
Note: When cleaning and disinfecting, always observe the safety instructions and hygiene
regulations.
5. Re-assemble the unit by reversing s
Decontamination
Decontamination in CelCulture uses 90°C moist heat to kill common organisms that
contaminates workspace including all installed components. The entire program run of the
decontamination routine takes approximately 15 hours (overnight process. The chamber is clean
and dry at the end of the cycle. No further wipe down is necessary.