Esco Isotherm IFC-110-8 User manual

Isotherm
User
Manual
Thank you for purchasing this Esco Low Temperature BOD
Incubator. Please read this manual thoroughly to familiarize
yourself with the many unique features and exciting
innovations we have built into your new equipment. Esco
provides many other resources at our website,
www.escoglobal.com, to complement this manual and help you
enjoy many years of productive and safe use of your Esco
products.
Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16 • Hatboro, PA 19040, USA
Toll-Free USA and Canada 888-375-ESCO
Tel [phone] • Fax [phone]
us.escoglobal.com • [e-mail]
Esco Micro Pte. Ltd.
21 Changi South Street 1 • Singapore 486 777
Tel [phone] • Fax [phone]
www.escoglobal.com • [e-mail]

Copyright Information
© Copyright 2011 Esco Micro Pte. Ltd. All rights reserved.
The information contained in this manual and the accompanying product is copyrighted and all rights are
reserved by Esco.
Esco reserves the right to make periodic minor design changes without obligation to notify any
person or entity of such change.
Isotherm®are registered trademarks of Esco.
“Material in this manual is provided for informational purposes only. The contents and the product described in
this manual (including any appendix, addendum, attachment or inclusion), are subject to change without
notice. Esco makes no representations or warranties as to the accuracy of the information contained in this
manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related
to the use of this manual.”

Low Temperature BOD Incubator
i
i
Table of Contents
iii
Warranty Terms and Conditions
v
Introduction
v
1. Products Covered
v
2. Safety Warning
v
3. Limitation of Liability
v
4. European Union Directives on WEEE and RoHS
vii
Declaration of Conformity
1
Chapter 1 –Product Information
1
1.1 About Isotherm Low Temperature BOD Incubator
1
1.2 Labels
2
1.3 Quick View
2
1.3.1 Front Quick View
3
1.3.2 Back Quick View
3
1.4 Applications
5
Chapter 2 –Installation
5
2.1 General Requirement
5
2.1.1 Location Requirements
5
2.1.2 Environmental Requirements
6
2.1.3 Power Requirements
6
2.2 Installation
6
2.2.1 Caster Wheel
6
2.2.2 Water Container
6
2.3 Disclaimer
7
Chapter 3 –Control System and Operation
7
3.1 Control System
7
3.1.1 Function Keys
7
3.1.2 LED Indicators
7
3.2 Unit Operation
7
3.2.1 Switching ON/OFF
7
3.2.2 Opening/Closing the Outer Door
8
3.2.3 Setting Temperature
8
3.2.4 Protection Devices
8
3.2.5 Auto-Defrosting
8
3.2.6 Placement of Loads
9
3.3 Menu & Options
10
3.3.1 Alarm Setting
10
3.3.2 Timer Setting
10
3.3.3 Password (PIN) Setting
11
3.3.4 Address Setting
11
3.3.5 UV Lamp
12
3.3.6 Defrost Setting
12
3.4 Calibration
12
3.6 Quick Keys
13
Chapter 4 –General Maintenance
13
4.1 Scheduled Maintenance
13
4.2 Cleaning
13
4.2.1 The Glass Door
14
4.2.2 Control Panel, Exterior & Interior Surfaces, Screen Frame, Temperature
Probe

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14
4.2.3 Bottom panel
14
4.2.4 Work Zone Decontamination
14
4.3 Physical Inspection
14
4.3.1 Re-Adjustment of Outer Door
15
4.4 Removal of Deposits
15
4.5 UV Lamp Replacement
15
4.6 Maintenance/Service Logs
17
Chapter 5 –Troubleshooting
Appendix

Low Temperature BOD Incubator
iii
Esco products come with either a 1, 2 or 3 year limited warranty, depending on the product purchased,
beginning on the date of shipment from any Esco international warehousing location. To determine which
warranty applies to your product, refer to the appendix below.
Esco's limited warranty covers defects in materials and workmanship. Esco's liability under this limited
warranty shall be, at our option, to repair or replace any defective parts of the equipment, provided if proven
to the satisfaction of Esco that these parts were defective at the time of being sold, and that all defective parts
shall be returned, properly identified with a Return Authorization.
This limited warranty covers parts only, and not transportation/insurance charges.
This limited warranty does not cover:
Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you
must file a claim directly with the freight carrier.
Products with missing or defaced serial numbers.
Products for which Esco has not received payment.
Problems that result from:
oExternal causes such as accident, abuse, misuse, problems with electrical power, improper
operating environmental conditions.
oServicing not authorized by Esco.
oUsage that is not in accordance with product instructions.
oFailure to follow the product instructions.
oFailure to perform preventive maintenance.
oProblems caused by using accessories, parts, or components not supplied by Esco.
oDamage by fire, floods, or acts of God.
oCustomer modifications to the product
Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs.
Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment. If the
objects are highly valuable, user is advised to have in place independent external preventive
measures such as connection to a centralized alarm system.
Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by
the original manufacturer. The customer agrees that in relation to these products purchased through Esco, our
limited warranty shall not apply and the original manufacturer's warranty shall be the sole warranty in respect
of these products. The customer shall utilize that warranty for the support of such products and in any event
not look to Esco for such warranty support.
Esco encourages all users to register their equipment online at www.escoglobal.com/warranty or complete the
warranty registration form included with each product.
ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED
WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR
IMPLIED, WILL APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY
BEYOND THE REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT,
CONSEQUENTIAL OR INCIDENTAL DAMAGES, INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-
PARTY CLAIMS AGAINST YOU FOR DAMAGES, FOR PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST
WORK. ESCO'S LIABILITY WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE
SUBJECT OF A CLAIM. THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE.

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Isotherm
These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and
shall be subject to the exclusive jurisdiction of the courts of Singapore.
Technical Support, Warranty Service Contacts
USA: 1-877-479-3726
Singapore: [phone]
Global Email Helpdesk: supp[email protected]m
Visit http://www.escoglobal.com/ to talk to a Live Support Representative
Distributors are encouraged to visit the Distributor Intranet for self-help materials.
Product Appendix, Warranty Listings
Biological Safety Cabinets, Laminar Flow Cabinets,
HEPA-Filtered Cabinets (except Streamline brand)
The warranty periods for BSC may vary by country. Contact
your local distributor for specific warranty details.
Laboratory Fume Hoods
1 year limited.
Ductless Fume Hoods
3 years limited for Ascent Opti's, 5 years for Ascent Max's.
Cleanroom Equipment
1 year limited.
Laboratory Ovens and Incubators
1 year limited.
CO2Incubators
2 years limited.
Containment/Pharma Products
2 years limited.
Ultralow Temperature Freezer
3 years limited. 60 months on Compressor.
The warranty period starts two months from the date your equipment is shipped from Esco facility for
international distributors. This allows shipping time so the warranty will go into effect at approximately the
same time the equipment is delivered to the user. The warranty protection extends to any subsequent owner
during the warranty period. Distributors who stock Esco equipment are allowed an additional four months for
delivery and installation, providing the product is registered with Esco. User can register product online at
www.escoglobal.com/warranty or complete the warranty registration form included with each product.
Policy updated on 12th Apr 2011 (This limited warranty policy does not apply to products purchased before 12th
Apr 2011)

Low Temperature BOD Incubator
v
1. Products Covered
Isotherm Low Temperature BOD Incubator (IFC)
Electrical
110L
240L
220-240 VAC 50/60 Hz 1 Φ
IFC-110-8
IFC-240-8
2. Safety Warning
Anyone working with, on or around this equipment should read this manual. Failure to read,
understand and follow the instructions given in this documentation may result in damage to the unit,
injury to operating personnel, and / or poor equipment performance.
Any internal adjustment, modification or maintenance to this equipment must be undertaken by
qualified service personnel.
The use of any hazardous materials in this equipment must be monitored by an industrial hygienist,
safety officer or some other suitably qualified individual.
Before you process, you should thoroughly understand the installation procedures and take note of
the environmental / electrical requirements.
In this manual, important safety related points will be marked with the symbol.
If the equipment is used in a manner not specified by this manual, the protection provided by this
equipment may be impaired.
3. Limitation of Liability
The disposal and / or emission of substances used in connection with this equipment may be governed by
various local regulations. Familiarization and compliance with any such regulations are the sole responsibility
of the users. Esco’s liability is limited with respect to user compliance with such regulations.
4. European Union Directive on WEEE and RoHS
The European Union has issued two directives:
• Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE)
This product is required to comply with the European Union’s Waste Electrical &
Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following
symbol:
Esco sells products through distributors throughout Europe. Contact your local Esco
distributor for recycling/disposal.
• Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS)
With respect to the directive on RoHS, please note that this hood falls under category 8 (medical devices) and
category 9 (monitoring and control instruments) and is therefore exempted from requirement to comply with
the provisions of this directive.

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Isotherm

Low Temperature BOD Incubator
vii
Declaration of Conformation
In accordance to EN ISO/IEC 17050-1:2010
We, Esco Micro Pte. Ltd.
of 21 Changi South Street 1
Singapore, 486777
Tel: [phone]
Fax: [phone]
declare on our sole responsibility that the product:
Category : Low Temperature BOD Incubator
Brand : Isotherm
Model : IFC-110-8, IFC-240-8
in accordance with the following directives:
2006/95/EEC : The Low Voltage Directive and its amending directives
92/31/EEC : The Electromagnetic Compatibility Directive and its amending
directives
has been designed to comply with the requirement of the following Harmonized Standard:
Low Voltage : EN 61010-1:2010
EMC : EN 61326-1:2006 Class B
More information may be obtained from Esco’s authorized distributors located within the European Union. A
list of these parties and their contact information is available on request from Esco.
_______________________________
XQ Lin
Group CEO, Esco
This Declaration of Conformity is only applicable for 230V AC 50/60Hz units

viii
Isotherm

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Low Temperature BOD Incubator
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1.1 About Isotherm Low Temperature BOD Incubator
Forced Convection
Convection is a method to transfer of heat energy that involves the movement of a fluid (gas or liquid). Fluid in
contact with the source of heat expands and tends to rise within the bulk of the fluid. Cooler fluid sinks to take
its place, setting up convection current. However, in a forced convection device, the fluid motion is generated
by an external source (like a pump, fan, suction device, etc.).
Forced Convection Laboratory Incubator
Laboratory Incubator is a device for controlling temperature and other condition in which a microbiological
culture is being grown. Typical incubator are insulated boxes with an adjustable heater, going up to 60˚C to
65˚C (140˚F to 150˚F), though some can go slightly higher (generally to no more than 100˚C
Incubator can vary in size from tabletop to units the size of small rooms. As for temperature, most commonly
used is approximately 36˚C to 37˚C. Most bacteria, especially the frequently used E. Coli, grow well under such
condition. For experimental organism, such as the budding yeast Saccharomyces cerevisiae, a growth
temperature of 30˚C is optimal.
Biochemical oxygen demand (BOD)
When organic matter decomposes, it is fed upon by aerobic bacteria. In this process, organic matter is broken
down and oxidized (combined with oxygen). Biochemical oxygen demand is a measure of the quantity of
oxygen used by these microorganisms in the aerobic oxidation of organic matter.
The BOD level is determined by subtracting this DO level from the DO level found in the original sample taken
five days previously:
BOD=mg/LDO(original sample)-mg/LDO(after incubation)
1.2 Labels
Model : The model of the unit
Serial : The serial number of the unit.
Power : The maximum power consumption and
the electrical requirement of the unit.
Manufacture in : The time of manufacture.

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Isotherm
1.3 Quick View
1.3.1 Front Quick View
1. Control panel.
2. Outer door - maintains temperature inside the equipment.
3. Outer door handle.
4. UV lamp switch -controls operation of UV lamp.
5. UV lamp - sterilizes inner chamber and loads.
6. Glass door - allows observation of loads and maintains temperature when the outer door is opened.
7. Glass door handle.
8. Inner chamber - maintains temperature and allows loads be put inside.
9. Castor wheel.
10. Water container -to collect the water from drain pan.
1
2
3
6
7
5
4
8
9
10

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Low Temperature BOD Incubator
1.3.2 Back Quick View
1. Electrical inlet -connects to power supply.
2. Main switch button -turns the equipment ON/OFF.
3. Outer door -maintains temperature inside unit.
4. Outer chamber - protects parts inside.
5. RS485 Port - RS485 connection.
1.4 Applications
It is commonly used as
Biochemical Oxygen Demand determinations of waste water and sewage (APHA method at 20oC)
Plant cell growth
Insect studies
Fermentation studies
Bacterial culturing
Mycology studies
Drug stability tests
1
2
3
4
5

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Isotherm

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Low Temperature BOD Incubator
–
2.1 General Requirement
2.1.1 Location Requirements
Isotherm Low Temperature BOD Incubator (IFC) must be placed on the solid and level surface. It is
important that the equipment is set up accurately horizontally and against abnormal vibration and
noise. Door may have to be adjusted. (See Chapter 4 “Maintenance and Cleaning”).
Lock the 2 front casters (by pushing down the pedals) to fix position of the equipment.
Do not place equipment outdoors, under sunlight, where be splashed onto the equipment, heavy
concentration of dust, moisture or close to inflammables!
NOTE: When the equipment is to be moved, release all the 2 front casters (by pull up the pedals) and fully support the
equipment while moving.
2.1.2 Environmental Requirements
Indoor use
Altitudes of up to 2000 meters (6600’)
Below 80% relative humidity –non condensing
Temperature between 15OC - 30OC (59OF - 86OF)
If ambient temperature fluctuation is more than 6oC, the control system will be reset according to its self-
inspection. Therefore control on temperature may be influenced.
Ensure stability of ambient temperature as temperature set point is close to ambient temperature. Especially
for temperature set point = ambient temperature+ 0~10oC, working in air-conditioning room is recommended;
otherwise non-erasable temperature fluctuation may be caused.
Technical specification for the equipment is specified for units with standard equipment at an ambient
temperature +25oC and a voltage fluctuation of ±10 %.
The temperature data are determined in accordance to factory standard following DIN 12 880.

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Isotherm
2.1.3 Power Requirements
Designed for 230VAC 50/60Hz 1Φelectrical rating
An unobstructed dedicated power outlet which is not shared with other appliances.
It is recommended that the voltage fluctuation doesn't exceed ±10% of the nominal voltage at all
times, or else an electrical power stabilizer can be installed.
UPS is recommended for better protection.
2.2 Installation
2.2.1 Caster Wheel
NOTE: It is recommended to lock the 2 front casters before starting the equipment. Otherwise, dangerous may be caused
due to unexpected force as closing glass door / outer door.
2.2.2 Water Container
2.3 Disclaimer
The performance and safety of the incubator, while rigorously evaluated at the factory, cannot be guaranteed
once after transit and installation. Therefore the on-site testing is always recommended.
References for qualified Certifiers:
Esco Distributor
Esco (www.escoglobal.com)
Front casters with
pedals
Back casters
without pedals
Drainage
Drain pipe
Water container

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Low Temperature BOD Incubator
-
3.1 Control System
3.1.1 Function Keys
MENU
Enters menu options.
Switches between submenus.
SET TEMP
Enters temperature and time setting option under basic operation mode.
Enters submenu/save set parameters and withdraw submenu.
UP
Parameter value plus key.
DOWN
Parameter value minus key.
MUTE
Mute alarm of ambient condition
3.1.2 LED Indicators
REFRIGERATING
Electrical refrigerating output indicator
AUTO-DEFROST
Auto-Defrost indicator.
UV LAMP
UV lamp indicator
ALARM
Alarm indicator
AMBIENT ALERT
Ambient condition indicator
3.2Unit Operation
3.2.1 Switching ON/OFF
The equipment is switched ON by pressing the main switch downward; pressing the main switch upward will
turn it OFF. The power supply will cut off and the operation will stop.
Actual
Display
Set Point
Display
LED Indicator
Function
Keys
Over Temperature
Indicator

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Isotherm
3.2.2 Opening/Closing Outer Door
Pull-down the handle to open the door, and pull-up to close the door.
NOTE: As there are 2 knobs at the top and bottom of the outer door, the outer chamber may be damaged by the knobs if
door handle is pulled-up during closing the door.
3.2.3 Setting Temperature (0oC –100oC)
Step
Description
Button
Display
1
Press SET TEMP button to start the setting.
SET TEMP

2
Press UP/DOWN button to set the temperature set point. Press SET
TEMP to save setting
UP/DOWN –
SET TEMP

NOTE:
If the ambient temperature does not meet requirement (15oC~30oC), the AMBIENT ALERT LED will light up and a
buzzer will sound every 20 sec as warning. The buzzer can be stopped by pressing the MUTE button.
Refrigerating system’s compressor will start working according to temperature set point and ambient
temperature. REFRIGERATING LED will light up when the equipment is in the refrigeration process.
UV LAMP LED will light up when the UV lamp is on.
3.2.4 Protection Devices
This is a non-adjustable temperature protection. The factory set value 110oC maximum. This electronic over-
temperature protection system is monitored by Protection Class 3.1 to DIN 12 880.If temperature in inner
chamber exceeds 110oC, heating within equipment will be stopped. As a warning, the OVER TEMPERATURE
LED will light up.
If this happens:
Switch off the equipment or even cut off the power supply and allow equipment to cool down.
Rectify the fault - contact customer service for assistance.
Re-start the device after the equipment is cooled down and fault has been rectified.
3.2.5 Auto-Defrosting
Auto-defrosting will be operated every 6 hours as temperature set point ≤ 11oC and every 24 hours as
temperature set point ≥ 11oC.Temperature will slightly fluctuate during defrosting.
Auto-defrosting will fail when the temperature in the inner chamber is or close to 0oC. If necessary,
use manual-defrost.
Every time user completes a test without refrigeration and next step wants to do the test with
refrigeration system running, please select heating-defrost program (or set 50oC) and open the door
for at least 30 minutes. Without this process, the auto-defrost performance may be affected
3.2.6 Placement of Loads
Full considerations must be given to physical and chemical properties of your load in order to prevent serious
damage to load, equipment and surroundings.
Note:
Isotherm Low Temperature BOD Incubator (IFC) is not explosion-proof, thus it is not suitable for
drying, evaporating and burning-in of paints, enamels or similar materials whose solvents may
produce an inflammable mixture with air.
There must be no possibility of the formation of inflammable gas / air mixtures either within the
equipment or in the immediate surroundings of the equipment.
Large amount of dust or corrosive fumes inside the equipment or in the surroundings of the
equipment may produce deposits within the equipment and lead to short circuits or damage of
electronics. Therefore it is important that adequate precautions are taken against excessive dust or
corrosive fumes.
In order to ensure good ventilation within equipment, the space for placing loads should not exceed
half volume of workroom (refer to “Technical Specification Summary Table”). Do not divide workroom
into several separate parts by the loads with larger area.

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Low Temperature BOD Incubator
There must be sufficient spacing of the load inside the equipment to ensure proper ventilation within
the equipment. Do not place any load on the floor, against the side wall or underneath the ceiling of
the equipment.
In order to ensure optimal ventilation, shelf should be inserted so that air spacing between shelves,
door, load and inner chamber wall is approximately equal.
Maximum quantity and loading of shelves can be seen in “Technical Specification Summary Table”.
Improper loading may increase the time on reaching the set temperature.
3.3 Menu & Options
Basic setting

Alarm setting


Timer setting


Password setting


Address setting


Timer setting of manual-operation on UV lamp


Timer setting of auto-operation on UV lamp


Defrosting setting


Program operation


Calibration


System Parameter
Display
(Diagnostic Mode)

Version number

.
AD sampling value


Present value

.
Set value

.
Upper limit of measuring range

.
Lower limit of measuring range

.
Historic record of temperature




Factory Settings




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Isotherm
3.3.1 Alarm Setting
The high point temperature alarm setting can be adjusted. Please note the alarm value is a relative setting.
Range for alarm setting: 2°C~10°C (If the refrigeration system is active, use 2oC). In case, actual temperature in
equipment is equal or higher than temperature set point + alarm value, buzzer will sound and red light of
"Alarm" indicator will flash.
Step
Description
Button
Display
1
Press MENU button until the display shows Setting. Press SET TEMP to
enter the menu.
MENU –
SET TEMP

2
Press MENU button until the display shows Alarm.
MENU

3
Press UP/DOWN button to choose the alarm value. Press SET TEMP to
save setting.
UP/DOWN –
SET TEMP
3.3.2 Timer Setting
3.3.2.1 Turning the timer ON and OFF
Step
Description
Button
Display
1
Press MENU button until the display shows Setting. Press SET TEMP to
enter the menu.
MENU –
SET TEMP

2
Press MENU button until the display shows Timer.
MENU

3
Press UP/DOWN button to set the timer ON/OFF. Press SET TEMP to save
setting.
UP/DOWN –
SET TEMP
3.3.2.2 Set timer (0000 –9999 seconds)
Time is set in normal operation, not in main menu. So exit the menu to set timer.
Step
Description
Button
Display
1
Press SET TEMP button to start the setting.
SET TEMP

2
Press MENU button until the display shows Timer.
MENU

3
Press UP/DOWN button to set required time. Press SET TEMP to save
setting.
UP/DOWN –
SET TEMP
NOTE:
The timer starts when the actual temperature is equal/larger than the set point temperature. Do not turn off the
timer, once it starts.
The remaining time can be checked using DOWN button during normal operation.
 is shown on “Actual” display and buzzer sounds 4 times as counting ends. All buttons are locked. Re-start the
device to reactivate the buttons.
Timer will not be saved and assumed to be  as equipment is re-started.
3.3.3 Password (PIN) Setting
The PIN consists of 4 alphanumeric digits. When set to 0000, the PIN is disabled; otherwise the MENU and SET
TEMP buttons functions require PIN.
3.3.3.1 Set PIN (0000 –9999; 0000 –pin disabled)
Step
Description
Button
Display
1
Press MENU button until the display shows Setting. Press SET TEMP to
enter the menu.
MENU –
SET TEMP

2
Press MENU button until the display shows PIN.
MENU

3
Press UP/DOWN button to set the PIN’s digit. Press SET TEMP to confirm
the number and move to the next digit.
UP/DOWN –
SET TEMP
4
Repeat step 3 until the fourth digit. Once the fourth digit is chosen,
pressing SET TEMP button will save the PIN.