Hach LANGE LICO 500 User manual

DOC027.53.00792
LICO 500
USER MANUAL
March 2007 Edition 1
©Hach Lange GmbH, 2007. All rights reserved. Printed in Germany.
sd/sk

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1
Table of contents
Section 1 Specifications ........................................................................................................................................ 5
Section 2 General Information .............................................................................................................................. 7
2.1 Safety information .............................................................................................................................................. 7
2.1.1 Use of hazard information ......................................................................................................................... 7
2.1.2 Precautionary labels ................................................................................................................................. 7
2.1.3 Chemical and Biological Safety ................................................................................................................ 7
2.2 Overview of product ........................................................................................................................................... 8
Section 3 Installation ............................................................................................................................................. 9
3.1 Unpack the instrument ....................................................................................................................................... 9
3.2 Environment considerations ............................................................................................................................... 9
3.3 Power connections ........................................................................................................................................... 10
3.4 Interfaces ......................................................................................................................................................... 11
3.5 Cell compartments and cell adapter ................................................................................................................ 12
3.5.1 Cell compartments and adapter .............................................................................................................. 12
3.5.2 Installation of the cell adapter ................................................................................................................. 13
3.6 Beam path ........................................................................................................................................................ 14
Section 4 Start Up ................................................................................................................................................ 15
4.1 Power the instrument on and off ...................................................................................................................... 15
4.2 Language selection .......................................................................................................................................... 15
4.3 Self-Check ....................................................................................................................................................... 15
4.4 Characteristics in continuous operation ........................................................................................................... 16
Section 5 Standard Operations ........................................................................................................................... 17
5.1 Overview .......................................................................................................................................................... 17
5.1.1 Tips for the use of the touch screen ........................................................................................................ 17
5.1.2 Use of the alphanumeric keypad ............................................................................................................ 17
5.1.3 Main Menu .............................................................................................................................................. 18
5.2 Instrument Setup mode .................................................................................................................................... 19
5.2.1 Operator ID ............................................................................................................................................. 19
5.2.2 Sample ID ............................................................................................................................................... 20
5.2.3 Date and time .......................................................................................................................................... 22
5.2.4 Display and sound preferences .............................................................................................................. 22
5.2.5 Lamp control ........................................................................................................................................... 22
5.2.6 PC and printer ......................................................................................................................................... 24
5.2.6.1 Printer setup ............................................................................................................................... 24
5.2.6.2 PC setup ..................................................................................................................................... 26
5.2.6.3 Print data .................................................................................................................................... 27
5.2.7 Password ................................................................................................................................................ 28
5.2.7.1 Deactivate a password ............................................................................................................... 29
5.2.8 Select color ............................................................................................................................................. 30
5.3 Store, recall, send and delete data .................................................................................................................. 31
5.3.1 Store, recall, send and delete data from the color log and data log ........................................................ 31
5.3.1.1 Auto/manual data storage ........................................................................................................... 31
5.3.1.2 Recall stored data from the color log or the data log ..................................................................31
5.3.1.3 Send data from the color log or the data log ............................................................................... 33
5.3.1.4 Delete stored data from the color log or the data log ..................................................................35
5.3.2 Store, recall, send and delete data from wavelength scan and time course ........................................... 36
5.3.2.1 Data storage from wavelength scan or time course ................................................................... 36
5.3.2.2 Recall stored data from wavelength scan or time course ........................................................... 36
5.3.2.3 Send data from wavelength scan or time course ........................................................................ 37
5.3.2.4 Delete stored data from wavelength scan or time course ........................................................... 39
5.4 Sampling and sample preparation ................................................................................................................... 40

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Table of Contents
5.5 Color measurement ......................................................................................................................................... 41
5.5.1 Take a color measurement ..................................................................................................................... 42
5.5.1.1 Touch-sensitive areas on the measurement window .................................................................. 43
5.5.1.2 Parameter setup options ............................................................................................................. 44
5.5.1.3 Change the color scale after a measurement .............................................................................45
5.5.1.4 Change the measuring range after a measurement ................................................................... 46
5.5.2 Take a Pharm. Eur. color measurement ................................................................................................. 46
5.5.3 Take a US Pharmacopoeia color measurement ..................................................................................... 50
5.5.4 Determine the Iodine color value ............................................................................................................ 50
5.5.5 Determine the Hazen color value (Pt-Co or APHA-method) ...................................................................51
5.5.6 Determine the Gardner color value ......................................................................................................... 51
5.5.7 Determine the Klett color number ........................................................................................................... 51
5.5.8 Mineral oil color value (ASTM D 1500 and ISO 2049) ............................................................................ 51
5.5.9 Determine the Saybolt color number (ASTM D 156) .............................................................................. 52
5.5.10 AOCS Cc 13 e (Lovibond®) scale ........................................................................................................ 52
5.5.11 Determine the Yellowness-Index (ASTM D 1925) ................................................................................ 52
5.5.12 Hess-Ives color number ........................................................................................................................ 52
5.5.13 The ADMI color number ........................................................................................................................ 53
5.6 Color difference measurement ......................................................................................................................... 54
5.6.1 Take a color difference measurement .................................................................................................... 54
5.6.1.1 View graph/table/values .............................................................................................................. 56
5.6.2 Take a color difference measurement with stored reference values ...................................................... 57
5.6.3 Add a reference to the reference list ....................................................................................................... 58
5.7 Photometry ....................................................................................................................................................... 59
5.7.1 Single Wavelength (absorbance, concentration and transmittance measurements) .............................. 59
5.7.1.1 Set up single wavelength mode .................................................................................................. 60
5.7.1.2 Take single wavelength measurements (single reading) ............................................................ 62
5.7.1.3 Take single wavelength measurements (continuous readings) .................................................. 62
5.7.2 Multi-Wavelength mode – measurements at more than one wavelength ............................................... 62
5.7.2.1 Set the reading mode at different wavelengths ..........................................................................62
5.7.2.2 Complete a measurement in the multi wavelength mode ........................................................... 65
5.7.3 Wavelength scan mode – recording of absorbance and transmission spectrums .................................. 65
5.7.3.1 Set up the wavelength scan ........................................................................................................ 66
5.7.3.2 Wavelength scan reading ........................................................................................................... 68
5.7.3.3 Work with reference scans ......................................................................................................... 70
5.7.4 Time course of absorbance/transmittance .............................................................................................. 71
5.7.4.1 Time course setup parameters ................................................................................................... 71
5.7.4.2 Time course scan reading .......................................................................................................... 73
5.7.4.3 Analysis of time course data ....................................................................................................... 74
5.7.4.4 Navigation of a time scan or a time scan analysis ......................................................................74
Section 6 Advanced Operations ......................................................................................................................... 75
6.1 System checks ................................................................................................................................................. 75
6.1.1 Instrument information ............................................................................................................................ 75
6.1.2 Upgrade of the instrument software ........................................................................................................ 76
6.1.3 Optical checks ......................................................................................................................................... 76
6.1.3.1 Verification kit ............................................................................................................................. 76
6.1.4 Output checks ......................................................................................................................................... 78
6.1.5 Lamp history ........................................................................................................................................... 78
6.1.5.1 Factory service ........................................................................................................................... 79
6.1.6 Service time ............................................................................................................................................ 79
6.1.7 Instrument backup .................................................................................................................................. 81

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Table of Contents
Section 7 Maintenance ........................................................................................................................................ 85
7.1 Cleaning requirements ..................................................................................................................................... 85
7.1.1 Housing and cell compartment ............................................................................................................... 85
7.1.2 Display .................................................................................................................................................... 85
7.1.3 Cuvettes/sample cells ............................................................................................................................. 85
7.2 Lamp replacement ........................................................................................................................................... 86
7.3 Filter pad maintenance .................................................................................................................................... 88
7.3.1 Filter pad replacement ............................................................................................................................ 88
Section 8 Troubleshooting .................................................................................................................................. 91
Section 9 Replacement Parts .............................................................................................................................. 93
9.1 Replacement parts ........................................................................................................................................... 93
Section 10 Contact Information .......................................................................................................................... 95
Section 11 Warranty, liability and complaints ................................................................................................... 97
Index ...................................................................................................................................................................... 99

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5
Section 1 Specifications
Specifications are subject to change without notice.
Performance specifications
Operating mode Color measurement, color difference measurement, Absorbance and
Concentration
Colorimetric evaluation All color values calculated for standard illuminant C of ASTM E 308 and a
2°standard observer.
Source lamp Gas-filled Tungsten (visible)
Wavelength range 320–1100 nm
Wavelength accuracy ± 1.5 nm (wavelength range 340–900 nm)
Wavelength reproducibility ≤0.1 nm
Wavelength resolution 1 nm
Wavelength calibration Automatic
Wavelength range for color
measurement 380 to 720 nm step 10 nm
Scanning speed ≥ 12 nm/s (in steps of 1nm)
Spectral bandwidth 5 nm
Photometric measuring range ± 3.5 Abs (wavelength range 340–900 nm)
Photometric accuracy 5 m Abs at 0.0 to 0.5 Abs
1% at 0.50 to 2.0 Abs
Photometric linearity < 0.5% to 2 Abs
< = 1% at > 2 Abs with neutral glass at 546 nm
Stray light < 0.1% T at 340 nm with NaNO2
Data log 500 color measurements, 50 color reference values, 500 photometric
measurements, 20 wavelength scans, 20 time scans
Physical and environmental specifications
Width 368 mm (14.5 in.)
Height 144 mm (5.7 in.)
Depth 359 mm (14.1 in.)
Weight 6.4 kg (14.11 Ib)
Operating requirements 10–40 °C (50–104 °F), max. 80% relative humidity (non-condensing)
Storage requirements –40–60 °C (-40–140 °F) max. 80% relative humidity (non-condensing)
Additional technical data
Mains connection External power supply: 100-240V/50-60Hz (Input); 15V/30VA (output)
Interfaces
Use only screened cables with maximum length of 3 meters.
1 x USB type A
1 x USB type B
Enclosure rating IP3X
Protection Class Class II

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7
Section 2 General Information
2.1 Safety information
Please read this entire manual before unpacking, setting up or
operating this equipment. Pay attention to all danger, warning and
caution statements. Failure to do so could result in serious injury to
the operator or damage to the equipment.
To make sure that the protection provided by this equipment is not
impaired, do not use or install this equipment in any manner other
than that specified in this manual.
2.1.1 Use of hazard information
DANGER
Indicates a potentially or imminently hazardous situation
which, if not avoided, will result in death or serious injury.
WARNING
Indicates a potentially or imminently hazardous situation
which, if not avoided, could result in death or serious injury.
CAUTION
Indicates a potentially hazardous situation that may result in
minor or moderate injury.
Important Note: Indicates a situation which, if not avoided, may
cause damage to the instrument. Information that requires special
emphasis.
Note: Information that supplements points in the main text.
2.1.2 Precautionary labels
Read all labels and tags attached to the instrument. Personal injury
or damage to the instrument could occur if not observed. A symbol,
if noted on the instrument, will be included with a danger or caution
statement in the manual.
2.1.3 Chemical and Biological Safety
DANGER
Potential Chemical/ Biological Exposure Hazards. Handling
chemical samples, standards and reagents can be dangerous.
Users of this product are advised to familiarize themselves
with safety procedures and the correct use of chemicals, and
to carefully read all relevant Material Safety Data Sheets.
This symbol, if noted on the instrument, references the instruction manual for operation and/or safety information.
Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after
12 August of 2005. In conformity with European local and national regulations (EU Directive 2002/96/EC),
European electrical equipment users must now return old or end-of life equipment to the Producer for disposal at no
charge to the user.
Note: For return for recycling, please contact the equipment producer or supplier for instructions on how to return
end-of-life equipment, producer-supplied electrical accessories and all auxiliary items for proper disposal.

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General Information
Normal operation of this instrument may involve the use of
hazardous chemicals or biologically harmful samples.
• The user must observe all cautionary information printed on the
original solution containers and safety data sheet prior to their use.
• All waste solutions must be disposed in accordance with local and
national law.
• The type of protective equipment must be selected according to
the concentration and amount of the dangerous substance at the
specific workplace.
2.2 Overview of product
The LICO 500 is a VIS spectrophotometer with a wavelength range
of 320 to 1100 nm. The instrument comes with a complete set of
application programs and multi-language support.
The LICO 500 contains the following application modes: Color
scales (pre installed), Single Wavelength Mode, Multi-Wavelength
Mode, Wavelength Scan and Time Course Mode.
The LICO 500 provides digital readouts in direct concentration
units, absorbance or percent transmittance.
The LICO 500 can carry out an exact colorimetric evaluation in
conformity with ISO/ASTM standards with just a single
measurement and display the result in terms of traditional color
systems such as Iodine, Hazen or Gardner color numbers.
Besides the 20 color indexes, transmittance and absorbance can
be measured at individual wavelengths, so that the LICO 500 can
also be universally used for analytical purposes in the laboratory.

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Section 3 Installation
WARNING
Electrical and Fire Hazards. Use only the provided power
supply. Only qualified personnel should conduct the tasks
described in this section of the manual.
3.1 Unpack the instrument
The LICO 500 comes packaged with the following items:
•LICO 500
•Dust cover
•External power supply, including 4 adapter for EU, UK, USA
and AUS/China
•LICO 500 user manual
•Quick start guide LICO 500
Note: If any of these items are missing or damaged, contact the
manufacturer or a sales representative immediatly.
3.2 Environment considerations
The following conditions are necessary to make sure correct
instrument operation and accurate results:
•Place the instrument firmly on an even surface. Do not push
any objects under the instrument.
•Maintain an ambient temperature of 10 to 40 ºC (50 to 104 ºF)
for proper instrument operation.
•The relative humidity should be less than 80%; moisture should
not condense on the instrument.
•Leave at least a 15 cm (6 in.) clearance at the top and on all
sides for air circulation to avoid overheating of electrical parts.
•Do not operate or store the instrument in extremely dusty,
damp or wet locations.
•Keep the surface of the instrument, the cell compartment and
all accessories clean and dry at all times. Splashes or spills on
and in the instrument should be cleaned up immediately (see
section 7.1 on page 85).
•After a continuous operating time of more than 24 hour without
switching off the instrument we recommend to perform a new
calibration with a distilled water cuvette/sample cell.
•After a continuous operating time of more than 7 days without
switching off the instrument we recommend to switch off and on
the instrument to perform a new system check with filter
adjustment and lambda-calibration
Important Note: Protect the instrument from temperature
extremes, including heaters, direct sunlight and other heat sources.

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Installation
3.3 Power connections
Install the correct adapter plug on the supplied external power
supply (Figure 1). Slide the adapter on until it "clicks" into position.
Plug the external power supply cord into the connector on the back
panel of the instrument, then plug the supply into a power outlet
(100–240 V~ / 50–60 Hz). Press the power switch on the back of
the instrument to initialize power (Figure 2 on page 11).
Figure 1 Power adapter
1Power supply with EU adapter plug installed 3USA adapter plug
2UK adapter plug 4AUS/China adapter plug

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Installation
3.4 Interfaces
The LICO 500 has two USB interfaces as a standard feature,
located on the back of the instrument (Figure 2). The USB Type A
interface is used for communications with a printer, USB memory
stick or keyboard. A USB memory stick is used to update
instrument software.
The USB Type B interface is used for communications with a PC.
The optional Hach Data Trans software (see Section 9 on page 93)
must be installed on the PC for this use.
A USB hub may be used to connect several accessories at a time.
Note: USB cables must not be longer than 3 meters (10 feet).
These USB interfaces enable data and graphics to be output to a
Printer and a PC and upgrade instrument software (see
section 6.1.2 on page 76).
Figure 2 Interfaces
1USB type A 3Plug in power supply
2USB type B 4On/Off switch

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Installation
3.5 Cell compartments and cell adapter
3.5.1 Cell compartments and adapter
The LICO 500 has two cell compartments (Figure 3). Only one
cuvette/sample cell type at a time can be used for a measurement.
Cell compartment #1
•11-mm round cuvettes/sample cells
For measurements with 11mm round cuvettes/sample cells in
cell compartment #1 insert the adapter Z into cell compartment
#2.
Cell compartment #2
Cell compartment #2 uses adapters to accommodate different
cuvette/sample cell types.
•50-mm rectangular cuvettes/sample cells (can be inserted
directly into the cell compartment without using an adapter).
•Adapter Z: 10-mm square cuvettes/sample cells
Important Note: Be sure that the adapter Z is inserted proper into
the cell compartment. Press the adapter down until it snap into the
compartment.
Figure 3 Cell compartments
1Cell compartment #1 2Cell compartment #2

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Installation
3.5.2 Installation of the cell adapter
1. Open the cell compartment.
2. Insert the adapter for measurements with the round
cuvette/sample cell (11 mm) and/or 10 mm square
cuvette/sample cell so the arrow on top of the adapter points to
the left (Figure 4) and the orientation tab fits the groove in the
compartment opening.
Note: The arrow on top of the adapter indicates the direction of the light
beam path.
Figure 4 Cell adapter Z
1Adapter Z: 10 mm square cell adapter

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Installation
3.6 Beam path
Figure 5 shows the beam path of the LICO 500.
Figure 5 Beam path
1Tungsten lamp 9Filter wheel
2Entrance slit 10 Splitter mirror
3Heat-protection glass 11 Reference-element
4Grating 12 Cell compartment #2
5LED 13 Lens
6Chopper 14 Measurement element
7Exit slit 15 Cell compartment #1
8Lens

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Section 4 Start Up
4.1 Power the instrument on and off
1. Plug external power supply into an electrical outlet.
2. Press the power switch on the back of the instrument to
initialize power.
Note: Do not turn the instrument off and on in rapid succession. Always
wait about
20 seconds before turning the instrument on again, otherwise
the electronic and mechanical systems will be damaged.
4.2 Language selection
The LICO 500 software includes several language options. The first
time the instrument is turned on, the language selection screen will
appear.
1. Select the desired language.
2. Press OK to confirm. The self-check will start automatically.
Changing the language setting
The instrument functions in the selected language until the option is
changed.
1. While turning the instrument on, touch the screen at any point
until the list for selecting a language appears (about
30 seconds).
2. Select the required language.
3. Press OK to confirm. The self-check will start automatically.
4.3 Self-Check
Each time the instrument is powered, a series of diagnostic tests
are performed automatically to make sure operation of major
system components.
This procedure, which takes approximately two minutes, checks the
system, lamp, filter adjustment, wavelength calibration and voltage.
Each test which functions correctly is confirmed with a check mark.
Note: For further error messages during self-check, see Section 8 on page
91.

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Start Up
The Main Menu is displayed when diagnostics are completed.
See section 5.1.3 Main Menu on page 18 for a detailed description.
4.4 Characteristics in continuous operation
After a continuous operating time of more than 24 hour without
switching off the instrument we recommend to perform a new
calibration with a distilled water cuvette/sample cell.
After a continuous operating time of more than 7 days without
switching off the instrument we recommend to switch off and on the
instrument to perform a new system check with filter adjustment
and lambda-calibration.

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Section 5 Standard Operations
5.1 Overview
5.1.1 Tips for the use of the touch screen
The entire screen is touch-activated. To make a selection, press the
screen with a fingernail, fingertip, pencil eraser or a stylus. Do not
press the screen with a sharp object, such as the tip of a ball
point pen.
•Do not place anything on top of the screen, to prevent damage
or scratching on the screen.
•Press keys, words or icons to select them.
•Use scroll bars to move up and down long lists very quickly.
Press and hold the scroll bar, then move up or down to move
through the list.
•Highlight an item from a list by pressing it once. When the item
has been successfully selected, it will be displayed as reversed
text (light text on a dark background).
5.1.2 Use of the alphanumeric keypad
This display is used to enter letters, numbers and symbols as
needed when programming the instrument. Unavailable options are
disabled (grayed out). The icons on the right and left of the screen
are described in Table 1.
The central keypad changes to reflect the selected entry mode.
Press a key repeatedly until the desired character appears on the
screen. A space can be entered by using the underscore on the
YZ_ key.
Note: A USB keyboard (with US keyboard layout) or a USB Barcode
handset scanner can be used for input (see Section 9 on page 93).
Table 1 Alphanumeric keypad
Icon / key Description Function
ABC/abc Alphabetic When entering alphabetic characters (ex. user-entered units), this key allows to
toggle between upper and lower case letters.
# % Symbols Punctuation, symbols and numerical sub- and superscripts may be entered.
123 Numeric For entering regular numbers.
CE Clear Entry Clear the entry.
Left Arrow Backspace Moves back one position. This deletes the character previously entered in the new
position.
Right Arrow Advance Moves to the next space in an entry when two adjacent characters occur on the
same key.

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Standard Operations
5.1.3 Main Menu
A variety of modes may be selected from the Main Menu. Table 2
briefly describes each menu option.
Table 2 Main Menu options
Option Function
Color Measurement
The COLOR MEASUREMENT mode is used to determine visual color values like Hazen,
Gardner, Saybolt as well as three-dimentional absolute colorimetric values like CIE Lab, Hunter
Lab or European Pharmacopoeia skale.
Color Difference
Measurement
The COLOR DIFFERENCE MEASUREMENT mode is used to determine a quantitative color
difference between a reference (R) and a sample (S) in the three-dimensional color space (CIE
L*a*b* or Hunter Lab). In this mode, an additional reference memory for up to 50 references is
available.
Photometry
SINGLE WAVELENGTH
Single wavelength measurements are:
Absorbance measurements: The light absorbed by the sample is measured in absorbance
units.
Transmittance measurements (%): The percentage of the light that passes through the sample
and reaches the detector is measured.
Concentration measurements: A concentration factor can be entered to enable the measured
absorbance values to be converted into concentration values.
MULTI WAVELENGTH
In the multi-wavelength mode, absorbance (Abs) or percentage transmittance (%T) is measured
at up to four wavelengths and absorbance differences and absorbance relationships are
calculated. Simple conversions into concentrations can also be carried out.
TIME COURSE
The time scan records the absorbance or % transmittance at a wavelength over a defined time.
WAVELENGTH SCAN
A wavelength scan shows how the light from a sample is absorbed over a defined wavelength
spectrum.This function can be used to determine the wavelength at which the maximum
absorbance value can be measured.The absorbance behavior is displayed graphically during the
scan.
System Checks The system checks menu offers a number of options, including optical checks, output checks,
lamp history, instrument update, service time and instrument backup.
Recall Data Stored data can be recalled, filtered, sent to Printer, Memory stick or PC and deleted.
Instrument Setup In this mode, user-specific or method-specific settings can be entered: Operator-ID, Sample-ID,
Date & Time, Display & Sound, Lamp Control, PC & Printer, Password and Select Color.