Hanna Instruments HI96101C User manual

INSTRUCTION MANUAL
HI96101C
pH, Chlorine, Cyanuric Acid,
Iodine, Bromine, Iron LR ISM

Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of
the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e‑mail us at
[email protected] or view our worldwide contact list at www.hannainst.com.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent
of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
Dear
Customer,

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PRELIMINARY EXAMINATION .............................................................................................
GENERAL DESCRIPTION .....................................................................................................
ABBREVIATIONS ...............................................................................................................
SPECIFICATIONS ...............................................................................................................
PRECISION AND ACCURACY ...............................................................................................
PRINCIPLE OF OPERATION ................................................................................................
FUNCTIONAL DESCRIPTION ...............................................................................................
ERRORS AND WARNINGS ..................................................................................................
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ..............................................................
STARTUP ..........................................................................................................................
RANGE SELECTION ............................................................................................................
MEASUREMENT PROCEDURE .............................................................................................
VALIDATION PROCEDURE ...................................................................................................
CALIBRATION PROCEDURE .................................................................................................
GLP .................................................................................................................................
BATTERY MANAGEMENT ....................................................................................................
BATTERY REPLACEMENT ....................................................................................................
ACCESSORIES ...................................................................................................................
TABLE OF CONTENTS
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Please examine this product carefully. Make sure that the instrument is not damaged. If any damage
occurred during shipment, please contact your local Hanna Instruments Office.
Each HI96101 Ion Selective Meter is supplied complete with:
• Sample Cuvettes and Caps (2 pcs.)
• CAL Check™ standard cuvettes
• 9V Battery
• Scissors
• Cloth for wiping cuvettes
• Instrument quality certificate
• Instruction Manual and Quick Reference Guide
• Rigid carrying case
Note: Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
PRELIMINARY EXAMINATION

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The HI96101 is an auto diagnostic portable microprocessor meter that benefits from Hanna
Instruments’ years of experience as a manufacturer of analytical instruments. It has the advanced
optical system based on a special tungsten lamp and a narrow band interference filter that allows
most accurate and repeatable readings. All instruments are factory calibrated and the electronic
and optical design minimizes the need of frequent calibration.
With the powerful CAL Check™ validation function, you are able to validate good performance
of your instrument at any time. The validation procedure is extremely user friendly. Just use the
exclusive Hanna Instruments ready‑made, NIST traceable standards to verify the performance of
the instrument and recalibrate if necessary.
All instruments are splash proof and the lamp and filter units are protected from dust or dirt by
a transparent cup. This makes the instruments fulfill field applications. Display messages aid the
user in routine operation. The meter has an auto‑shut off feature that will turn off the instrument
after 10 minutes of non use in measurement mode or after 1 hour if left in calibration mode.
The meter uses an exclusive positive‑locking system to ensure that the cuvette is in the same
position every time it is placed into the measurement cell. It is designed to fit a cuvette with a
larger neck making it easier to add both sample and reagents. The cuvette is made from special
optical glass to obtain best results.
The HI96101 meter measures pH, Free and Total Chlorine, Cyanuric Acid, Iodine, Bromine and Iron
Low Range content in water and wastewater samples in the following ranges:
The reagents are in liquid and powder form depending on the parameter and they are supplied
in dropper bottles and packets. The amount of reagent is precisely dosed to ensure the maximum
repeatability.
GENERAL DESCRIPTION
Parameter Range
pH 6.5 to 8.5 pH
Free Chlorine 0.00 to 5.00 mg/L
Total Chlorine 0.00 to 5.00 mg/L
Cyanuric Acid 0 to 80 mg/L
Iodine 0.0 to 12.5 mg/L
Bromine 0.00 to 10.00 mg/L
Iron LR 0.00 to 1.60 mg/L

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degree Celsius
degree Fahrenheit
milligrams per liter. mg/L is equivalent to ppm (parts per million)
milliliter
millivolts
US Environmental Protection Agency
°C:
°F:
mg/L:
mL:
mV:
USEPA:
ABBREVIATIONS
SPECIFICATIONS
Range pH
Free Chlorine
Total Chlorine
Cyanuric Acid
Iodine
Bromine
Iron LR
6.5 to 8.5 pH
0.00 to 5.00 mg/L
0.00 to 5.00 mg/L
0 to 80 mg/L
0.0 to 12.5 mg/L
0.00 to 10.00 mg/L
0.00 to 1.60 mg/L
Resolution 0.1 pH
0.01 mg/L under 3.50 mg/L Chlorine
0.10 mg/L above 3.50 mg/L Chlorine
1 mg/L Cyanuric Acid
0.1 mg/L Iodine
0.01 mg/L Bromine
0.01 mg/L Iron LR
Accuracy
@25 °C (77 °F)
pH
Free Chlorine
Total Chlorine
Cyanuric Acid
Iodine
Bromine
Iron LR
±0.1 pH
±0.03 mg/L ± 3% of reading
±0.03 mg/L ±3% of reading
±1 mg/L ± 15% of reading
±0.1 mg/L ± 5% of reading
±0.08 mg/L ± 3% of reading
±0.01 mg/L ± 8% of reading
Light Source Tungsten lamp
Light Detector Silicon Photocell with narrow band interference filter @525 nm

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Method For pH: Phenol red method. The reaction with the reagent causes a red tint
in the sample.
For Chlorine: Adaptation of the EPA, DPD method and Standard Method
4500‑Cl G. The reaction between chlorine and the reagent causes a pink tint
in the sample.
For Cyanuric Acid: Adaptation of the turbidimetric method. The reaction
between cyanuric acid and the reagent causes a white suspension in the
sample.
For Iodine: Adaptation of the EPA, DPD method. The reaction between iodine
and the reagent causes a pink tint in the sample.
For Bromine: Adaptation of the EPA, DPD method. The reaction between
bromine and the reagent causes a pink tint in the sample.
For Iron LR: Adaptation of the TPTZ method. The reaction between iron and
the reagent causes a violet tint in the sample.
Environment 0 to 50 °C (32 to 122 °F)
max 95 % RH non‑condensing
Battery Type 9V (1 pc.)
Auto‑Shut off After 10’ of non‑use in measurement mode;
after 1 hour of non‑use in calibration mode;
with last reading reminder
Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
Weight 320 g (11.3 oz.)
SPECIFICATIONS
REQUIRED REAGENTS
Code Parameter Description Quantity/test
HI93710‑0 pH Phenol red reagent 5 drops
HI93701‑0 Free Chlorine DPD Powder Reagent 1 packet
HI93711‑0 Total Chlorine DPD Powder Reagent 1 packet
HI93722‑0 Cyanuric Acid Powder Reagent 1 packet
HI93718‑0 Iodine DPD Powder Reagent 1 packet
HI93716‑0 Bromine DPD Powder Reagent 1 packet
HI93746‑0 Iron LR Powder Reagent 2 packet

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Precision is how closely repeated measurements
agree with each other. Precision is usually
expressed as standard deviation (SD).
Accuracy is defined as the nearness of a test
result to the true value.
Although good precision suggests good accuracy,
precise results can be inaccurate. The figure
explains these definitions.
In a laboratory using a standard solution of the
parameter and a representative lot of reagent
(for each parameter) the following standard
deviations were obtained:
PRECISION AND ACCURACY
Parameter Standard Solution Standard Deviation
pH 7.0 pH 0.1 pH units
Free Chlorine 1.00 mg/L 0.03 mg/L
Total Chlorine 1.00 mg/L 0.03 mg/L
Cyanuric Acid 20 mg/L 1 mg/L
Iodine 2.5 mg/L 0.1 mg/L
Bromine 2.00 mg/L 0.08 mg/L
Iron LR 0.80 mg/L 0.01 mg/L

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Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and
matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms,
molecules or crystal lattices.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
through the matter and on the physical‑chemical characteristics of the substance according to the
Lambert‑Beer Law:
‑log I/Io= ελc d
or
A = ελc d
Where:
‑log I/Io = Absorbance (A)
Io = intensity of incident light beam
I = intensity of light beam after absorption
ε
λ= molar extinction coefficient at wavelength λ
c = molar concentration of the substance
d = optical path through the substance
Therefore, the concentration “c” can be calculated from the absorbance of the substance as the
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound from a
specific chemical reaction between sample and reagents. Given that the absorption of a compound
strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should
be selected as well as a proper central wavelength to optimize measurements.
The optical system of Hanna Instruments’ HI96 series colorimeters is based on special subminiature
tungsten lamps and narrow‑band interference filters to guarantee both high performance and
reliable results.
HI96 block diagram (optical layout)
PRINCIPLE OF OPERATION

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A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned
and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of
the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light
beam of intensity Ioor I.
The photoelectric cell collects the radiation Ioor I that is not absorbed by the sample and converts
it into an electric current, producing a potential in the mV range.
The microprocessor uses this potential to convert the incoming value into the desired measuring
unit and to display it on the LCD.
The measurement process is carried out in two phases: first the meter is zeroed and then the actual
measurement is performed.
The cuvette has a very important role because it is an optical element and thus requires particular
attention. It is important that both, the measurement and the calibration (zeroing) cuvettes, are
optically identical to provide the same measurement conditions. Whenever possible use the same
cuvette for both. It is necessary that the surface of the cuvette is clean and not scratched. This to avoid
measurement interference due to unwanted reflection and absorption of light. It is recommended
not to touch the cuvette walls with hands.
Furthermore, in order to maintain the same conditions during the zeroing and the measuring phases,
it is necessary to close the cuvette to prevent any contamination.
PRINCIPLE OF OPERATION