Hanna Instruments HI 98186 User manual

1
Dissolved Oxygen
BOD/OUR/SOUR
Meter
HI 98186
Instruction Manual
www.hannainst.com
HI 98186
Dissolved Oxygen Meter

2
WARRANTYWARRANTY
WARRANTYWARRANTY
WARRANTY
Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of
the instrument, as well as a precise idea of its versatility.
If you need additional technical information, do not hesitate to e-mail us at
[e-mail] or turn to the back cover for our worldwide contact list.
These instruments are in compliance with directives.
The HI 98186 instrument is guaranteed for two years against defects in
workmanship and materials when used for its intended purpose and maintained
according to instructions. Electrodes and probes are guaranteed for six months.
This warranty is limited to repair or replacement free of charge.
Damage due to accidents, misuse, tampering or lack of prescribed maintenance
is not covered.
If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of purchase,
serial number and the nature of the problem. If the repair is not covered by
the warranty, you will be notified of the charges incurred. If the instrument is
to be returned to Hanna Instruments, first obtain a Returned Goods Authori-
zation number from the Technical Service department and then send it with
shipping costs prepaid. When shipping any instrument, make sure it is
properly packed for complete protection.

3
TABLE OF CONTENTSTABLE OF CONTENTS
TABLE OF CONTENTSTABLE OF CONTENTS
TABLE OF CONTENTS
WARRANTY ......................................................................................... 2
PRELIMINARY EXAMINATION ................................................................ 4
GENERAL DESCRIPTION ........................................................................ 4
FUNCTIONAL DESCRIPTION .................................................................. 6
SPECIFICATIONS.................................................................................. 8
PROBE FUNCTIONAL DESCRIPTION ....................................................... 9
PROBE CONNECTION AND PREPARATION............................................. 10
OPERATIONAL GUIDE......................................................................... 11
OUR MEASUREMENT ......................................................................... 21
SOUR MEASUREMENT ....................................................................... 24
TEMPERATURE MEASUREMENT ............................................................ 27
DO CALIBRATION PROCEDURE ............................................................ 27
GOOD LABORATORY PRACTICE (GLP) ................................................... 32
SETUP .............................................................................................. 33
LOGGING .......................................................................................... 45
AutoEnd ........................................................................................... 48
PRESSURE CALIBRATION.................................................................... 49
TEMPERATURE CALIBRATION .............................................................. 51
PC INTERFACE ................................................................................... 54
BATTERY RECHARGER/REPLACEMENT .................................................... 62
PROBE MAINTENANCE ....................................................................... 64
TROUBLESHOOTING GUIDE ................................................................ 65
ACCESSORIES .................................................................................... 66

4
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
PRELIMINARY EXAMINATIONPRELIMINARY EXAMINATION
PRELIMINARY EXAMINATION
The HI 98186 is a state of art, microprocessor based, dissolved oxygen
instrument with many automated and specific application features, designed
to provide laboratory results and accuracy under harsh industrial conditions.
All measurements are automatically compensated for temperature. Salinity
compensation in water allows direct determination of dissolved oxygen in
saline waters. With its internal barometer, the instrument is able to automati-
cally compensate for changes in barometric pressure so there is no need for
charts, altitude information or external barometric pressure information.
The instrument contains built-in application software for the calculation of
Biochemical Oxygen Demand (BOD), Oxygen Uptake Rate (OUR) and Specific
Oxygen Uptake Rate (SOUR).
HI 98186’s calibration has been greatly simplified when compared to other
dissolved oxygen instruments. It is provided with a series of new features which
add entirely new dimensions to the measurement of DO, by allowing the user
to dramatically improve the reliability of the measurement:
• One or two points automatic dissolved oxygen calibration.
• One point manual dissolved oxygen calibration using a value in milligrams
per liter or percent saturation entered by the user.
• One or two points user temperature calibration.
• Messages on the graphic LCD for an easy and accurate calibration.
• User-selectable “calibration time out” to remind when a new calibration is
necessary.
Remove the instrument from the packing material and examine it carefully to
make sure that no damage has occurred during shipping. If there is any
damage, notify your Dealer or the nearest Hanna Customer Service Center.
Each instrument is supplied with:
• HI 76407/4F Polarographic probe with built-in temperature sensors and
4 m (13.4”) cable
• HI 76407A Membrane cap (2 pcs)
• HI 7041S Electrolyte solution (30 mL)
• 4 x 1.2V AA, 1300 mAh Rechargeable Batteries (inside the instrument)
• HI 710042 Inductive Recharger with power adaptor
• Instruction Manual
• Rugged carrying case
Note: Save all packing material until you are sure that the instrument
functions correctly. All defective items must be returned in the original
packing with the supplied accessories.
GENERAL DESCRIPTIONGENERAL DESCRIPTION
GENERAL DESCRIPTIONGENERAL DESCRIPTION
GENERAL DESCRIPTION

5
Moreover, the meter offers an extended temperature range from
–20 ºC to 120 ºC (–4 ºF to 248 ºF).
Other features include:
• Log on demand up to 400 samples.
• Auto Hold feature, to freeze first stable reading on the LCD.
• GLP feature, to view last calibration data.
• PC interface.
Hanna Instruments reserves the right to modify the design,
construction and appearance of its products without advance notice.

6
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
FUNCTIONAL DESCRIPTIONFUNCTIONAL DESCRIPTION
FUNCTIONAL DESCRIPTION
1) Liquid Crystal Display (LCD).
2) F1, F2, F3 functional keys.
3) / keys to manually increase/decrease the parameters or to scroll the pa-
rameter list.
4) ESC key to leave current mode, exit calibration, setup, help, etc.
5) CAL key, to enter/exit calibration mode.
6) RCL key, to enter/exit view logged data mode.
7) SETUP key, to enter/exit SETUP mode.
8) ON/OFF key, to turn the instrument ON and OFF.
9) GLP key, to display Good Laboratory Practice information.
10) LIGHT key, to toggle display backlighting.
11) MODE key to change DO measuring unit when in DO measure screen, or to
toggle between standard and pressure in DO calibration.
12) RANGE key, to switch between DO, BOD, OUR AND SOUR.
13) Help key to enter/exit contextual help.
Front viewFront view
Front viewFront view
Front view

7
Top viewTop view
Top viewTop view
Top view
DOUSB
14
15
14) Electrode DIN connector.
15) USB connector.

8
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SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS

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PROBE FUNCTIONAL DESCRIPTIONPROBE FUNCTIONAL DESCRIPTION
PROBE FUNCTIONAL DESCRIPTIONPROBE FUNCTIONAL DESCRIPTION
PROBE FUNCTIONAL DESCRIPTION
PROBE FUNCTIONAL DESCRIPTIONPROBE FUNCTIONAL DESCRIPTION
PROBE FUNCTIONAL DESCRIPTIONPROBE FUNCTIONAL DESCRIPTION
PROBE FUNCTIONAL DESCRIPTION
1. D.O. Probe
2. Protective Cap
3. Watertight Shielded Cable
4. Polypropylene Probe Body
5. Temperature Sensor
6. O-Ring Seal
7. Silver Chloride Anode
8. Platinum Cathode (sensor)
9. Oxygen Permeable Teflon®Membrane
10. Membrane Cap

10
To take measurements, connect the D.O. probe to the meter securely by
aligning the pins with the socket located on the top of the meter, pushing the
plug in and tightening the threaded ring.
Probes shipped from Hanna Instruments
are dry. To hydrate the probe and prepare
it for use, connect it to the meter and
proceed as follows:
1. Remove the red and black plastic cap.
This cap is for shipping purposes and can
be thrown away.
2. Wet the sensor by soaking the bottom
2½ cm (1") of the probe in electrolyte
(HI 7041S) for 5 minutes.
3. Rinse the membrane cap (HI 76407A)
supplied in the kit with the meter) with
electrolyte solution while shaking it gently.
Refill with clean electrolyte solution.
4. Tap gently the sides of the membrane
cap with your finger tip to ensure that no
air bubbles are trapped. To avoid damag-
ing the membrane, do not tap it directly
on the bottom.
5. Make sure that the rubber O-ring sits
properly inside the membrane cap.
6. With the sensor facing down, slowly
screw the cap clockwise. Some electrolyte
will overflow.
When not in use and during polarization
(see PROBE CONDITIONING page 11), use the protective transparent cap
supplied in the kit with the meter.
PROBE CONNECTION AND PREPARATIONPROBE CONNECTION AND PREPARATION
PROBE CONNECTION AND PREPARATIONPROBE CONNECTION AND PREPARATION
PROBE CONNECTION AND PREPARATION

11
INITIAL PREPARATION
The instrument is supplied complete with rechargeable batteries. Proceed with
a complete charging process before starting (see page 60).
To prepare the instrument for field measurements close the serial communica-
tion socket and all unused connector sockets with proper stopper (to ensure
waterproof protection).
Connect the DO probe to the 7-pin connector. Make sure that the probe sleeve
is properly inserted and tighten the threaded ring.
Turn the instrument ON by pressing ON/OFF key.
At start-up the display will show the Hanna logo for a few seconds , followed by
the percentage indication of the remaining battery charge, then enters the
probe conditioning mode. The probe will be conditioned for one minute, and
afterwards the instrument will enter measurement mode.
If the DO probe is not connected or is damaged, the conditioning period is
skipped.
To save battery life, the auto-off feature turns the instrument off after a set
period (default 30 min) if no button is pressed. To set another period or to
disable this feature, see SETUP menu on page 33.
The auto-off backlight feature turns the backlight off after a set period (default
1 min) with no buttons pressed. To set another period or to disable this
feature, see SETUP on page 33.
OPERATIONAL GUIDEOPERATIONAL GUIDE
OPERATIONAL GUIDEOPERATIONAL GUIDE
OPERATIONAL GUIDE
PROBE CONDITIONING
At startup, the probe is under polarization with a fixed voltage of approxi-
mately 800 mV for 1 minute.
Probe polarization is essential for stable measurements with the same recur-
ring degree of accuracy.
With the probe properly polarized, oxygen is continually consumed when it
passes through the sensitive diaphragm and dissolves in the electrolyte solu-
tion contained in the probe.
Whenever measurements are taken with a non-polarized probe, the oxygen
level revealed is both that of the tested solution, as well as that present in the
electrolyte solution. This reading is incorrect.
Keep the protective cap on during polarization time and remove it for calibra-
tion and measurements.

12
SALINITY COMPENSATION
If the sample contains significant concentration of salinity, the read out values
must be corrected, taking into account the lower degree of oxygen solubility in
this situation.
Before taking any DO measurements remember to set the salinity value from
the SETUP menu (page 33).
The salinity affects the D.O. concentration, decreasing its value. The table below
shows the maximum oxygen solubility at various temperatures and salinity levels.
Cº
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Fº
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006.4146.3147.2109.1105.110.23
218.3119.2170.2192.1119.015.63
490.3152.2174.1137.0183.012.93
644.2156.1119.0122.0198.98.24
838.1190.1104.0157.944.94.64
0182.1185.0139.923.930.90.05
2177.0111.0105.929.856.86.35
4192.0186.901.955.803.82.75
6168.982.937.812.879.78.06
8154.909.893.809.766.74.46
0280.965.870.806.783.70.86
2237.832.877.733.721.76.17
4204.839.794.770.778.62.57
5242.897.763.759.657.60.77
6290.856.732.738.646.68.87
8218.783.789.616.624.64.28
0345.741.757.693.622.60.68
2392.709.645.691.630.66.98
4350.786.633.610.658.52.39
6328.674.641.638.586.58.69
8316.682.669.566.515.54.001
0414.690.697.505.563.50.401
2422.639.536.553.522.56.701
4440.677.584.512.590.52.111
6478.516.533.570.579.48.411
8407.574.502.559.458.44.811
0545.533.570.538.457.40.221
NoteNote
NoteNote
Note:The relationship between salinity and chlorinity for sea water is given by the equation
below:
Salinity (g/l) = 1.80655 Chlorinity (g/l)

13
BAROMETRIC PRESSURE COMPENSATION
Cº
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m
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m
06.411.416.311.316.211.217.112.118.014.010.017.93.90.99.80.23
28.313.318.214.219.115.110.116.012.019.95.92.98.85.84.86.53
41.316.212.217.113.119.015.011.017.93.90.97.84.80.89.72.93
64.210.215.111.117.013.019.96.92.99.86.82.89.76.75.78.24
88.114.110.116.012.018.95.91.98.84.81.88.75.73.72.74.64
013.119.015.011.017.94.90.97.84.81.88.75.72.79.68.60.05
218.014.010.016.93.99.86.83.80.87.74.71.79.66.65.66.35
413.019.96.92.99.85.82.89.76.74.71.78.66.63.62.62.75
619.95.92.98.85.82.89.76.73.70.78.65.63.61.60.68.06
815.91.98.85.81.88.76.73.70.78.65.63.60.68.57.54.46
021.98.84.81.88.75.73.70.77.65.62.60.68.56.55.50.86
227.84.81.88.75.72.70.77.65.62.60.68.56.54.53.56.17
424.81.88.75.72.70.77.65.62.60.68.56.54.52.51.52.57
523.80.87.74.71.78.66.64.61.69.57.55.53.51.50.50.77
621.88.75.72.70.77.65.62.60.68.56.54.52.50.59.48.87
828.75.73.70.77.65.62.60.68.56.54.52.50.58.47.44.28
036.73.70.78.65.63.60.68.56.54.52.50.58.46.46.40.68
233.70.78.65.63.61.68.56.54.52.50.58.47.45.44.46.98
431.78.66.63.61.69.56.54.52.50.59.47.45.43.43.42.39
638.66.63.61.69.57.55.53.51.59.47.45.44.42.41.48.69
836.64.61.69.57.55.53.51.59.47.45.44.42.41.40.44.001
044.62.69.57.55.53.51.59.47.46.44.42.41.49.39.34.401
242.60.68.56.53.52.50.58.46.44.43.41.40.48.38.36.701
440.68.56.54.52.50.58.46.45.43.41.40.48.37.37.32.111
648.56.54.52.50.58.47.45.43.42.40.49.37.36.35.38.411
847.55.53.51.59.47.45.44.42.40.49.37.36.35.34.34.811
055.53.51.59.47.46.44.42.41.49.38.36.35.34.33.30.221
The dissolved oxygen saturation value varies with pressure, so it is important
to compensate the effect that pressure has on DO measurements.

14
The HI 98186 meter contains a built-in barometer, and it is able to automati-
cally compensate for changes in barometric pressure. If another pressure value
than the barometer’s reading is to be used, then the manual pressure feature
must be enabled from the SETUP menu (see page 33), and afterwards the
pressure value can be set using the ARROW keys.
The table below contains a conversion altitude (m) to pressure (mmHg) for the
altitude values from the previous table.
DO MEASUREMENTS
Make sure the probe’s protective cap has been removed.
In order to take accurate dissolved oxygen measurements make sure that the
instrument has been calibrated (see page 27 for details).
Press RANGE to access the DO measure screen. If necessary press MODE to
change the measuring unit.
Immerse the tip of the probe into the sample to be tested. Allow approxi-
mately one minute for the reading to stabilize (hourglass symbol turns off).
On the screen are displayed:
• Dissolved Oxygen reading in the selected unit (% saturation or mg/L)
• Temperature reading in the selected unit (°C or °F)
• Pressure reading in the selected unit (mmHg, inHg, atm, psi, kPa, mbar).
If the Manual Pressure option is enabled ( displayed in front of the pressure
value) the pressure value can be changed using the ARROW keys.
For accurate dissolved oxygen measurements, a water movement of
0.3 m/s is required. This is to ensure that the oxygen-depleted membrane
surface is constantly replenished. A moving stream will provide adequate
circulation.
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erusserP
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15
BOD MEASUREMENTS
Biochemical oxygen demand (BOD) is an indicator for the concentration of
biodegradable organic matter present in a sample of water. It can be used to
infer the general quality of the water and its degree of pollution. BOD
measures the rate of oxygen uptake by microorganisms in a sample of water
at a fixed temperature and over a given period of time. To ensure that all
other conditions are equal, a very small amount of microorganism seed is
added to each sample being tested. This seed is typically generated by
diluting activated sludge with deionized water. The samples are kept at 20 ºC
in the dark and tested for dissolved oxygen (DO) after five days. The loss of
dissolved oxygen in the sample, once correction have been made for the degree
of dilution, is called the BOD5
Before measuring BOD, remember to set the BOD configuration from the
SETUP menu (see page 33).
Press RANGE to access the BOD measure screen.
Press BOD Data to view the BOD initial data management screen.
Press Compute to evaluate the BOD for a specified sample (available only
when the measurement is stable and at least one initial BOD data record has
been memorized).
BOD initial data management screen
BOD Data is pressed while in BOD measurement screen.
Press Add new to add a new BOD initial data record (the key is available only
when the measurement is stable). A 200 records memory space is available
for BOD initial data.
Press View ID to view the saved BOD initial values (the key is available only

16
when at least one initial BOD data record has been memorized).
By pressing Add New the BOD Parameters screen will be displayed:
BOD Parameters:
•Bottle ID: a number used to identify a specific bottle.
Range: 0000 to 9999.
•The type of the Sample: Sample or Seed.
•Bottle Volume: the total volume of the BOD bottle.
Range: 0.1 to 300.0 mL.
•Sample Volume: the volume of sample in the BOD bottle.
Range: 0.1 to 300.0 mL (for a seed sample this value is 0.0 mL and
cannot be set).
•Seed Volume: the volume of seed in the BOD bottle.
Range: 0.0 to 300.0 mL.
Press Prev/Next to select a different parameter on the screen.
Press ARROW keys to modify the selected parameter’s value.
Press Save to save the BOD parameters and the initial DO, temperature,
pressure and salinity values for the specified bottle.
If a bottle with the same ID already exists, the instrument will ask for
replacement confirmation.
Press Replace to replace the existing record, or ESC to return to the previous
screen without replacing.
When a new record is saved the meter will display a message indicating the
remaining free BOD initial data space in %.

17
By pressing View ID a list of all the saved BOD initial data records will be
displayed. The seed records will have the symbol “*” displayed after the bottle
ID.
Use the ARROW keys to scroll the list of BOD initial data records.
Press More to view detailed information for the selected record.
Press PgUp/Pg/Down to view the next/previous screen of information.
Use the ARROW keys to view the detailed information about the
next/previous record.
If Delete is pressed.
Use ARROW keys to focus on the record to be deleted and then press CFM.
Press ESC to exit.
If Delete All is pressed the instrument asks for confirmation. Press CFM to
confirm or ESC to exit without deleting.

18
BOD evaluation
From the BOD measure screen press Compute to evaluate the BOD for a
specified sample. The following screen will be displayed.
If the date of the current measurement is previous to the date of the selected
measurement then the Eval. BOD key will not be displayed.
Press MORE to view detailed information for the selected record.
Use ARROW keys to select the bottle for BOD evaluation.
Press Eval. BOD to compute the BOD for the selected bottle. If the time
difference between the current reading and the selected reading is less than
1 day the instrument will ask for record replacement confirmation, and the
BOD can’t be evaluated.
Press CFM to replace the selected record’s DO, temperature, pressure and
salinity values with the current values.
Press ESC to return to the previous screen without replacing.
If the conditions regarding the time difference are met, after pressing the
Eval.BOD key, the instrument will display the computed BOD value.
Press LOG to save the BOD result.
Press ESC to return to the BOD measure screen.
If the final DO reading or DO loss don’t meet the criteria for BOD measure-
ments set from the instrument’s setup, a warning message will be displayed.

19
Press any key to clear the warning message from the display or press HELP to
view detailed information about the warning.
Note: If the Autodelete BOD start data option is enabled in SETUP
(see page 33), when the BOD result is saved (LOG key pressed) the
corresponding BOD initial data record will be automatically deleted
from the instrument’s memory.
Seed Correction
In case that the BOD was evaluated for a seeded sample and the list of the
saved seed BOD values is not empty, the Correct functional key will be
displayed.
Press Correct to view the list of the saved seed BOD values.
Select the desired seed BOD and then press Correct to compute the corrected
BOD value. The instrument will display the corrected BOD value.
If the information about the BOD of a certain seed doesn’t exist at the moment
of the BOD evaluation for a seeded sample, the sample’s BOD can be corrected
at a later time from the BOD recall menu (view logged BOD data).
In order to perform a seed correction from the BOD recall, press RCL key from
the BOD measurement screen to enter BOD recall, select the desired BOD
record and press More. The instrument will display a complete set of informa-
tion about the selected record.
Press Correct to view the list of the seed values.
Select the desired seed BOD and then press Correct to compute the corrected
BOD value. The new BOD value will be displayed.

20
Note: If the final DO value is greater than the initial DO value an error
message will be displayed.