SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
Instruction Manual
www.hannainst.com
DearCustomer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the
test kit. It will provide you with the necessary information
for correct use of the kit. If you need additional information,
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
• HI 93702-0 Reagent, packets (50 pcs);
• HI 3856-0 Reagent, packets (50 pcs);
• Deionized Water, 1 bottle (230 mL)
• 1 long path color comparator cube;
• 1 color comparator cube;
• 1 plastic test tube (14 mL) with screw cap;
• 1 plastic pipette (3 mL).
Note: Any damaged or defective item must be returned in
its original packing materials.
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
HI 38075
Copper
Low and High Range
Test Kit
ISTR38075 02/00 PRINTED IN ITALY
Range 0 to 0.25 mg/L (ppm)
0 to 6.0 mg/L (ppm)
Smallest Increment 0.05 ppm
1.2 ppm
AnalysisMethod Colorimetric,bicinchoninate
Sample Size 25 mL
2.5 mL
Number of Tests 100 (50 for each range)
CaseDimensions 230x59x70 mm (9.0x2.3x2.8")
Shipping Weight 555 g (19.6 oz.)
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USE
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTION
Copper salts react with bicinchoninate reagent to form a
purple product in a neutral buffered condition. The amount
of color developed is proportional to the concentration of
copper present in the aqueous sample.
Copper is an essential trace element in human diet (the
daily requirement is around 2.0 mg) and a factor in plant
metabolism. Copper salts are used in water supply systems
tocontrolbiologicalgrowthinreservoirs. High concentrations
on the other hand are toxic and corrosion of copper alloys in
pipe fittings may introduce considerable quantities into the
water supplies.
Note: mg/L is equivalent to ppm (parts per million).
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONS
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• To measure copper in the 0-0.25 range, fill the long
path comparator cube with sample up to the 25 mL
mark.
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Health and Safety
Data Sheet before performing this test.
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETY
REFERENCESREFERENCES
REFERENCESREFERENCES
REFERENCES
Adaptation of EPA approved method.
• Add 1 packet
of HI 3856-0
reagent.
• Replace the cap and mix by
invertingthe cube severaltimes.
• Wait 45 seconds to allow color to
develop.
• Determine which
colorbestmatches
thesolution inthe
cube and read
the result directly
in mg/L (ppm) of
Copper.
• It is better to
match the color
with a white
sheet placed at
about 10 cm (4") behind the comparator.
• To measure copper in the 0-6.0
range, use the pipette to fill the
plastic test tube with 2.5 mL of
sample, up to the mark.
• Add 3.5 mL of
DeionizedWater to
reach the 6 mL
mark. Replace the
cap and swirl to
mix. This is the
diluted sample.
• Pour then 5 mL of the di-
luted sample from the tube
to the color cube (up to the
mark).
• Add 1 packet of HI 93702-0, replace
the cap and shake well.
• Wait 45 seconds to
allowcolortodevelop.
• Determinewhich color matchesthesolution in thevessel.
Multiply the result by 2.4 to obtain mg/L (or ppm) of
Copper in the sample.
• It is better to match the color with a white sheet placed
at about 10 cm (4") behind the comparator.
2.5 mL
25 mL
